Madiha Khan - Academia.edu (original) (raw)

Papers by Madiha Khan

iRASD Journal of Energy & Environment

Globalization has substantial influence on the environmental issues and consequently on the econo... more Globalization has substantial influence on the environmental issues and consequently on the economic activities of a country. Environmental problems are more prominent in developing countries. With the growing development, the issue of environmental degradation is also enhanced. The non-renewable energy consumption is the main cause of environmental degradation and this problem can’t be ignored. Therefore, the main objective of this research is to analyze the connection among energy consumption, growth of an economy, and emission of carbon dioxide gas in Pakistan. The data is collected from World development Indicators. Autoregressive Distributed-Lag Model (ARDL) is used for the analysis. The outcomes of ARDL technique stipulate that energy consumption and growth of an economy rise the emission of CO2 in Pakistan. On the basis of these results, it is suggested that policy makers in Pakistan must implement and encourage different sources of energy, which are renewable and will suppor...

Open Life Sciences

Lactobacilli (n= 24), isolated from human infants and yogurt, showed variablein vitroactivity aga... more Lactobacilli (n= 24), isolated from human infants and yogurt, showed variablein vitroactivity againstSalmonella enteritidis(8.0 ± 1.0 to 16.6 ± 0.5 mm) and other gut pathogens (9.0 ± 1.0 to 15.3 ± 0.5 mm), as determined by a well diffusion assay. The isolates were identified asLimosilactobacillus fermentum(FY1, FY3, FY4, IL2, and IL5),Lactobacillus delbrueckii(FY6 and FY7),Lactobacillussp. (IL7), andLactobacillus gasseri(IL12). All isolates showed variablein vitrotolerance to acidic pH for 3 h and visible growth at pH 4 and in the presence of 0.3% ox-bile. The antibiotic susceptibility profile ofLactobacillusisolates indicated resistance against vancomycin, ciprofloxacin, streptomycin, and lincomycin. Isolates had variable auto-aggregation and showed variable capabilities to co-aggregate withS. enteritidis. Based on all tested parameters,L. fermentumIL2,L. fermentumIL5, andL. gasseriIL12 were selected for co-culture experiments, followed byin vivoevaluation in Balb/c mice. All the s...

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: The aim of the present study is to evaluate various pharmacognostic standards like mac... more Objective: The aim of the present study is to evaluate various pharmacognostic standards like macroscopy, microscopy, fluorescence analysis, preliminary phytochemical screening and thin layer chromatography of Moringa oleifera (Lamk.) and Jasminum sambac (L.) Ait. Methods: Cellular arrangement of the flower of M. oleifera and J. sambac were examined under electronic microscope. Fine powder of both flowers was used for powder microscopy. The phytochemical screening of the aqueous and ethyl acetate extracts of flowers of M. oleifera and J. sambac was performed for carbohydrates, terpenoids, alkaloids, flavonoids, saponins, tannins, steroids, lignin and fixed oil. The color intensity or the precipitate formation was used as analytical responses to these tests. The aqueous and ethyl acetate extracts of both plants were gone through TLC monitoring by reconstituting each extracts with 5 ml solvent respectively. Results: Under microscopic observation the dried powder of M. oleifera reveale...

Paul Tabb: constructed and characterized bop1 bop2 pny pnf, knat6 pny pnf, ath1-3 pny pnf lines a... more Paul Tabb: constructed and characterized bop1 bop2 pny pnf, knat6 pny pnf, ath1-3 pny pnf lines and associated control lines, analyzed BOP2:GUS expression in the pny pnf IM, and made the PRXR9:GUS reporter construct. Alexander Edwards: constructed 35S:BOP1-GR lines with the help of Paul Tabb.

Pakistan Veterinary Journal, 2019

A feeding trial of indigenous probiotic lactobacilli strains including Lactobacillus gallinarum P... more A feeding trial of indigenous probiotic lactobacilli strains including Lactobacillus gallinarum PL 53, L. paracasei PL 120 and L. gallinarum PL 149 was conducted to monitor the effect on intestinal absorption capacity and histological changes in broiler chicks challenged with Campylobacter jejuni. A total of 45, day old chicks were randomly assigned to nine experimental groups (5 birds/ group). Group A was negative and B positive control while group C, D and E prevention model, F, G and H treatment model and group I antibiotic control. Groups other than negative control received Campylobacter jejuni (10 6 CFUs/bird) challenge on day 14 by oral gavage. The groups of prevention model received lactobacilli (~10 8 CFUs/bird) strains from day 1-35 and treatment model received lactobacilli from day 15-35. The absorption capacity of intestine was monitored by concentration of D-xylose in plasma (0.5 and 1 hour post administration). The probiotic group L. gallinarum PL 53 (group C) significantly enhanced D-xylose absorption capacity as compared to control groups (59.69±2.07mg/dL) in both the prevention (72.83±1.20mg/dL) and treatment (71.11±2.27mg/dL) models. Intestinal morphology was observed by measuring villi length, width, crypt depth and surface area of the small intestine. PL 53 increased the villi length of ileum (930±4.00µm), jejunum (890±8.00µm) and duodenum (1350±4.00µm). The group which received L. gallinarum PL 53 strain had maximum surface area of the intestinal segments with 0.41±0.03mm 2 , 0.45±0.03mm 2 and 0.72±0.02mm 2 of ileum, jejunum and duodenum, respectively. In conclusion, the PL 53 L. gallinarum may improve the gut performance and absorption capacity of broilers.

Solid State Sciences, 2018

Studies on magnetocapacitance, dielectric, ferroelectric, and magnetic properties of microwave si... more Studies on magnetocapacitance, dielectric, ferroelectric, and magnetic properties of microwave sintered (1-x) (Ba 0.8 Sr 0.2 TiO 3)-x (Co 0.9 Ni 0.1 Fe 2 O 4) multiferroic composite

Pakistan Journal of Zoology, 2019

Pure and Applied Biology, 2017

A filed experiment on "Morpho-agronomic and qualitative performance of various FCV tobacco exotic... more A filed experiment on "Morpho-agronomic and qualitative performance of various FCV tobacco exotic hybrids" was conducted at the Tobacco Research Station Khan Garhi Mardan during the year of 2014-2015. The experiment was designed in randomized complete block design with three replications. Ten (10) FCV tobacco hybrids (

Journal of Food Science and Technology, 2014

A total of 300 meat samples comprising mutton, beef, and chicken meat (n=100) collected from eith... more A total of 300 meat samples comprising mutton, beef, and chicken meat (n=100) collected from either local butcher shops or large meat outlets situated at various areas of Lahore City located in Punjab province of Pakistan were tested for the isolation of Clostridium perfringens. Prevalence of the organism was highest in the chicken (6 %) followed by mutton (5 %) and beef (1 %). Contamination level was high (10/150) in the samples collected from local butcher shops in comparison to the samples collected from large meat outlets (2/150). All of the raw meat samples were negative for the presence of alpha, beta and epsilon toxins of C. perfringens as detected through ELISA. Out of a total number of 12 isolates only half were capable of producing enterotoxins when cultured in trypticase glucose yeast (TGY) broth. Toxinotyping of the isolates showed that 3 were of type A while one each of the remaining three belonged to type B, C, and D. Antibiotic susceptibility testing of the toxin producing isolates revealed that C. perfringens were susceptible to chloramphenicol, ciprofloxacin, metronidazole, and ceftriaxone. All of the other drugs were relatively less effective with a least activity of amoxicillin against the isolates.

Journal of entomology and zoology studies, 2017

A field experiment was conducted to find out and compare the efficacy of Pyriproxyfen, Nitenpyram... more A field experiment was conducted to find out and compare the efficacy of Pyriproxyfen, Nitenpyram, Carbosulfan and Chlorpyrifos against aphid on Brassica juncea variety (Bahawalpur Raya) during Rabi 2016-17 at Regional Agricultural Research Institute, Bahawalpur, Pakistan. After 72 hours of treatment carbosulfan, nitenpyram and pyriproxyfen showed statistically similar results. Aphid population reduction of 96.7% was observed on the brassica plants treated with Carbosulfan, followed by 96.4% and 91.6% control of aphid infestation caused by Nitenpyram and Pryriproxyfen respectively. Chlorpyrifos showed 67.8% results.

Plant Protection, 2019

The present study was designed to determine the LC50 of some insecticides commonly used against H... more The present study was designed to determine the LC50 of some insecticides commonly used against Helicoverpa armigera and their comparative efficacy against the insect pest. The second instar larvae of H. armigera reared in the laboratory were selected for leaf dip bioassay. Two types of insecticides viz. conventional (deltamethrin and bifenthrin) and new chemistry (spinosad and indoxacarb) were assessed in the present studies. The results revealed that bifenthrin was more toxic to the second instar larvae of H. armigera at all the doses with lower LC50 value of 120.007 ppm as compared to deltamethrin with the highest LC50 value of 292.404 ppm. Among the new chemistry insecticides, indoxacarb proved to be more toxic than spinosad with LC50 of 5.592 ppm. LC50 of spinosad was 8.201 ppm showing 1.46 times less toxicity than indoxacarb.

Journal of Scientific Research, 2009

The conventional method for designing a fixed bed adsorption unit has been discussed. The method ... more The conventional method for designing a fixed bed adsorption unit has been discussed. The method is based on the data obtained from an adsorption column test. The characterization of an adsorption system, however, is performed in a laboratory batch experiment. It is shown that the conventional method does not make proper use of the physico-chemical parameters of an adsorption system determined by batch test. Also the method fails to predict the performance of an adsorption unit, if the operating condition differs from that under which the column test has been conducted for design purposes. New design equation has been proposed for both ‘Constantly Stirred Tank Reactor (CSTR)’ and ‘Plug Flow Reactor (PFR)’ type adsorption units. The equation predicts the performance of a reactor type adsorption unit under varying operating conditions. The proposed method is based only on the data obtained in batch experiment.Keywords: Adsorption; Unit design; Reactor; Optimal Operation, Dosage; Coeff...

Journal of Veterinary Advances, 2014

Foot and Mouth Disease (FMD) is one of the highly contagious diseases of domestic and wild animal... more Foot and Mouth Disease (FMD) is one of the highly contagious diseases of domestic and wild animals of many countries of the world including Bangladesh. The present study was undertaken to detect currently circulating FMD virus (FMDV) in Bangladesh using BHK-21 cell line, and the FMDV were typed using RT-PCR. The viruses induced characteristics cytopathic effects in BHK-21 cell lines, for examples, rounding, swelling, breaking down of intercellular bridge and cell death. Out of 151 clinical samples collected from different districts of Bangladesh, 85(56.29%) were successfully adapted in BHK-21 cell line, and were analyzed using FMDV type specific primers. Among these 85 BHK-21 adopted virus, 71(83.52%) were found as positive for FMD virus by RT-PCR; of which, 31 (36.47%) were positive for type A, 26 (30.58%) for type O, and 10 (11.76%) for type Asia 1. Only 4 (4.70%) samples were found as positive for mixed infection having Type A and Asia 1 FMDV. All the FMDV originated from Tangail (n=7) and Rajshahi (n=6) districts were found as positive for FMDV while 71.42% (n=14/36) samples of Mymensingh district were positive for FMDV. The FMDV identified in this study could now be used for effective vaccine development to control the disease in Bangladesh.

Scientific Reports, 2018

Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of p... more Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.

Plant Science, 2014

BLADE-ON-PETIOLE (BOP) genes encode an ancient and conserved subclade of BTB-ankryin transcriptio... more BLADE-ON-PETIOLE (BOP) genes encode an ancient and conserved subclade of BTB-ankryin transcriptional co-activators, divergent in the NPR1 family of plant defense regulators. Arabidopsis BOP1/2 were originally characterized as regulators of leaf and floral patterning. Recent investigation of BOP activity in a variety of land plants provides a more complete picture of their conserved functions at lateral organ boundaries in the determination of leaf, flower, inflorescence, and root nodule architecture. BOPs exert their function in part through promotion of lateral organ boundary genes including ASYMMET-RIC LEAVES2, KNOTTED1-LIKE FROM ARABIDOPSIS6, and ARABIDOPSIS THALIANA HOMEOBOX GENE1 whose products restrict growth, promote differentiation, and antagonize meristem activity in various developmental contexts. Mutually antagonistic interactions between BOP and meristem factors are important in maintaining a border between meristem-organ compartments and in controlling irreversible transitions in cell fate associated with differentiation. We also examine intriguing new evidence for BOP function in plant defense. Comparisons to NPR1 highlight previously unexplored mechanisms for coordination of development and defense in land plants.

Plant Signaling & Behavior, 2012

Inflorescence architecture varies widely among flowering plants, serving to optimize the display ... more Inflorescence architecture varies widely among flowering plants, serving to optimize the display of flowers for reproductive success. In Arabidopsis thaliana, internode elongation begins at the floral transition, generating a regular spiral arrangement of upwardly-oriented flowers on the primary stem. Post-elongation, differentiation of lignified interfascicular fibers in the stem provides mechanical support. Correct inflorescence patterning requires two interacting homeodomain transcription factors: the KNOTTED1-like protein BREVIPEDICELLUS (BP) and its BEL1-like interaction partner PENNYWISE (PNY). Mutations in BP and PNY cause short internodes, irregular spacing and/or orientation of lateral organs, and altered lignin deposition in stems. Recently, we showed that these defects are caused by the misexpression of lateral organ boundary genes, BLADE-ON-PETIOLE1 (BOP1) and BOP2, which function downstream of BP-PNY in an antagonistic fashion. BOP1/2 gain-of-function in stems promotes expression of the boundary gene KNOTTED1-LIKE FROM ARABIDOPSIS THALIANA6 (KNAT6) and shown here, ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1), providing KNAT6 with a BEL1-like co-factor. Our further analyses show that defects caused by BOP1/2 gain-of-function require both KNAT6 and ATH1. These data reveal how BOP1/2-dependent activation of a boundary module in stems exerts changes in inflorescence architecture.

Background: Limited data suggest that physical activity increases postexercise blood pressure in ... more Background: Limited data suggest that physical activity increases postexercise blood pressure in African-American women. The purpose of this study was to evaluate the postexercise blood pressure response to acute exercise in normotensive young adult African-American women. Methods: Eight healthy women (age 22.56.9 years) performed a cycle ergometer bout of 30 minutes at 60% of peak ventilatory oxygen uptake (VO 2 peak). Control arterial blood pressure, heart rate, lower leg blood flow, cardiac output, spectral analysis of blood pressure, heart rate variability, and baroreceptor sensitivity were measured for 5 minutes before exercise and were compared to postexercise measurements performed at rest intervals of 15-20, 35-40 and 55-60 minutes after exercise. Results: Exercise performed at 60% VO 2 peak produced an arterial pressure of 172610/ 70.164.0 mm Hg. Postexercise recovery values were not significantly different than the baseline control values. Conclusion: These results do not support the hypothesis that acute physical activity exerts an adverse effect on postexercise blood pressure in African American women. (Ethn Dis.

PLANT PHYSIOLOGY, 2011

The transition to flowering in many plant species, including Arabidopsis (Arabidopsis thaliana), ... more The transition to flowering in many plant species, including Arabidopsis (Arabidopsis thaliana), is marked by the elongation of internodes to make an inflorescence upon which lateral branches and flowers are arranged in a characteristic pattern. Inflorescence patterning relies in part on the activities of two three-amino-acid loop-extension homeodomain transcription factors: BREVIPEDICELLUS (BP) and PENNYWISE (PNY) whose interacting products also promote meristem function. We examine here the genetic interactions between BP-PNY whose expression is up-regulated in stems at the floral transition, and the lateral organ boundary genes BLADE-ON-PETIOLE1 (BOP1) and BOP2, whose expression is restricted to pedicel axils. Our data show that bp and pny inflorescence defects are caused by BOP1/2 gain of function in stems and pedicels. Compatible with this, inactivation of BOP1/2 rescues these defects. BOP expression domains are differentially enlarged in bp and pny mutants, corresponding to th...

Background: The purpose of this study was to determine the effect of smoking on speed and coordin... more Background: The purpose of this study was to determine the effect of smoking on speed and coordination of upper-limb movement. Methods: A total of 62 healthy, young individuals were randomly selected for participation in this study. Out of total subjects, 31 were smokers with a mean age 20.84 years and 31 were non-smokers with a mean age 19.97 years and placed in group A and group B, respectively. Plate taping test was administered to all the subjects to determine their speed and coordination of upper limb. Findings: Student’s t-test was applied between both groups to see the difference in their plate taping test’s time; its t-value was 5.03. Conclusion: Statistical significant difference was seen between both the groups in the result of plate taping test, which suggests that smoking can affect the speed and coordination of upper limb in a negative way and cause its deterioration. Therefore, immediate quitting from smoking is of high value and requirements to have a good speed and c...