Magdiel Lainez - Academia.edu (original) (raw)
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Papers by Magdiel Lainez
Renewable Energy, Aug 1, 2019
This work reports an improved process of enzymatic saccharification on Agave salmiana leaves and ... more This work reports an improved process of enzymatic saccharification on Agave salmiana leaves and its conversion into bioethanol comparing strains of Saccharomyces cerevisiae ethanol RED and Kluyveromyces marxianus OFF1. A. salmiana leaves were pretreated with acid-alkaline sequential process before the application of several treatments of enzymatic saccharification. In the results, the best enzymatic treatment reached conversion of 94.49%, releasing sugar concentrations of 50 g L À1. During fermentation, glucose consumption efficiencies were similar for both strains with values of 98%, while the consumption rates were higher for S. cerevisiae. Fermentative efficiencies were higher for K. marxianus with values of 92.88 ± 3.24% while for S. cerevisiae they were 87.63 ± 2.23%. These findings show a saccharification enzymatic process efficiently applied on A. salmiana leaves. K. marxianus, a non-Saccharomyces strain, converted all the sugars released from this plant structure into bioethanol with similar values or even higher than a Saccharomyces strain.
Biomass and Bioenergy, 2018
In this work, series of sequential acid-alkaline and enzymatic saccharification treatments were a... more In this work, series of sequential acid-alkaline and enzymatic saccharification treatments were applied on lignocellulosic biomass of Agave salmiana leaves in order to evaluate its conversion into simple sugars. The effects of a catalytic reagent and residence time were evaluated on lignocellulosic biomass. Also, several levels of FPU and cellulose loadings on the treated biomass were applied in the enzymatic saccharification. Upon completion of an acid-alkaline pretreatment, the resulting hemicellulose was completely hydrolyzed, whereas that lignin removed and the remaining cellulose reached values of 91 and 84% (w/w) respectively. The liquid by-product after acid pretreatment had a content of 0.50 g/L of glucose, 1.87 ± 0.045 g/L of arabinose, 21.35 g/L of xylose, and 39.15 g/L of reducing sugars. The saccharification process reached yields of 93.11% of conversion and glucose concentrations of 49.76 g/L. The sequential process was highly efficient in releasing simple sugars with concentrations of up to 88.91 g/L. This study provides data to improve the conversion processes of lignocellulosic components A. salmiana leaves into simple sugars, and illustrates the potential of using this crop's residue as a feedstock for biofuel production.
PLOS Computational Biology
Renewable Energy, Aug 1, 2019
This work reports an improved process of enzymatic saccharification on Agave salmiana leaves and ... more This work reports an improved process of enzymatic saccharification on Agave salmiana leaves and its conversion into bioethanol comparing strains of Saccharomyces cerevisiae ethanol RED and Kluyveromyces marxianus OFF1. A. salmiana leaves were pretreated with acid-alkaline sequential process before the application of several treatments of enzymatic saccharification. In the results, the best enzymatic treatment reached conversion of 94.49%, releasing sugar concentrations of 50 g L À1. During fermentation, glucose consumption efficiencies were similar for both strains with values of 98%, while the consumption rates were higher for S. cerevisiae. Fermentative efficiencies were higher for K. marxianus with values of 92.88 ± 3.24% while for S. cerevisiae they were 87.63 ± 2.23%. These findings show a saccharification enzymatic process efficiently applied on A. salmiana leaves. K. marxianus, a non-Saccharomyces strain, converted all the sugars released from this plant structure into bioethanol with similar values or even higher than a Saccharomyces strain.
Biomass and Bioenergy, 2018
In this work, series of sequential acid-alkaline and enzymatic saccharification treatments were a... more In this work, series of sequential acid-alkaline and enzymatic saccharification treatments were applied on lignocellulosic biomass of Agave salmiana leaves in order to evaluate its conversion into simple sugars. The effects of a catalytic reagent and residence time were evaluated on lignocellulosic biomass. Also, several levels of FPU and cellulose loadings on the treated biomass were applied in the enzymatic saccharification. Upon completion of an acid-alkaline pretreatment, the resulting hemicellulose was completely hydrolyzed, whereas that lignin removed and the remaining cellulose reached values of 91 and 84% (w/w) respectively. The liquid by-product after acid pretreatment had a content of 0.50 g/L of glucose, 1.87 ± 0.045 g/L of arabinose, 21.35 g/L of xylose, and 39.15 g/L of reducing sugars. The saccharification process reached yields of 93.11% of conversion and glucose concentrations of 49.76 g/L. The sequential process was highly efficient in releasing simple sugars with concentrations of up to 88.91 g/L. This study provides data to improve the conversion processes of lignocellulosic components A. salmiana leaves into simple sugars, and illustrates the potential of using this crop's residue as a feedstock for biofuel production.
PLOS Computational Biology