Magno Rodrigues de Carvalho Filho (original) (raw)
Papers by Magno Rodrigues de Carvalho Filho
Symbiosis, Jan 3, 2020
Trichoderma exhibits great ecological and agricultural relevance because it improves plant growth... more Trichoderma exhibits great ecological and agricultural relevance because it improves plant growth, development and productivity. In this study, Trichoderma isolates (T01, T02, T04, T74, T76 and T96 of T. asperelloides; T77 of T. asperellum; or T44, T78 and T92 of T. harzianum) were evaluated in vitro for their ability to solubilize phosphate and produce indole-3-acetic acid (IAA). Afterwards, a completely randomized experimental design with 12 treatments was used to investigate physiological changes in cowpea plants inoculated with Bradyrhizobium sp. BR 3267 (bradyrhizobia) or coinoculated with bradyrhizobia and those Trichoderma isolates in the greenhouse conditions. All Trichoderma isolates showed the ability to solubilize phosphate and to produce IAA. Cowpeas were positively influenced by coinoculation with bradyrhizobia and Trichoderma, with the highlight being cowpea plants coinoculated with bradyrhizobia and T. asperelloides T02. These plants display significant increases in height; relative growth rate; stem diameter; dry weight of shoots, roots and nodules; total dry weight; and specific root length in relation to other symbiotic pairs and absolute control. In contrast, negative responses were registered in cowpea plants coinoculated with bradyrhizobia and T. asperelloides T04, bradyrhizobia and T. harzianum T44 or bradyrhizobia and T. harzianum T92. These plants, together with the absolute control, display lower values of stem diameter, total dry weight, specific root length, and total chlorophyll and carotenoids; the absence of root nodules; and a higher root length and anthocyanin content in relation to other treatments. Our hypothesis is that the increase in root length may be related to IAA produced by Trichoderma, while anthocyanin accumulation is associated with nitrogen deficiency, suggesting that these plants are under stress. To our knowledge, this is the first evidence of antagonistic relationships between bradyrhizobia and Trichoderma in the Highlights • Adequate microbial consortium with Trichoderma may increase legume productivity. • T. asperelloides T02 induces positive responses on cowpea nodulated by bradyrhizobia. • The in vivo synergism among bradyrhizobia and Trichoderma is reported.
Comunicata Scientiae, Apr 6, 2017
The present study aimed to evaluate the Trichoderma asperellum inoculation efficiency in agronomi... more The present study aimed to evaluate the Trichoderma asperellum inoculation efficiency in agronomic performance of soybean plants under field conditions. Two experiments were conducted in a savanna in Gurupi, Tocantins, Brazil, during the 2013/2014 and 2014/2015 crop years, being realized soil chemical analysis and recording the climatic conditions. For the experiment, seeds from the cultivar Monsoy 9144 RR were used and the inoculation with T. asperellum were carried out with a powdered product with active ingredient based on Trichoderma (Trichoderma asperellum-UFT201), formulated with minimum viable concentration of conidia of 2x10 9 g-1 , applied directly on the seeds (20 g/kg seed), in one of the treatments. Were evaluated the biomass, relative efficiency, initial and final stand, effectiveness or efficiency in the use of T. asperellum in maintaining the stand and yield. It was observed that with the use of the treatment with T. asperellum inoculation, the yield of both harvests were 8.5 and 9.1% higher, respectively, when compared to the treatment with no inoculation.
Brazilian Journal of Botany, Jan 9, 2016
The aim of this study was to determine the efficiency of Trichoderma spp. in promoting the growth... more The aim of this study was to determine the efficiency of Trichoderma spp. in promoting the growth of cowpea plants. Strains were isolated from Tocantins savannah soils. Twenty-one isolates were evaluated in vitro for their ability to solubilize calcium phosphate and synthesize indole acetic acid (IAA). The soil samples were characterized physicochemically before planting. Eleven isolates were selected to evaluate the promotion of cowpea plant growth in the greenhouse in soil fertilized with phosphorite in 1.7-L pots. Then, at 32 and 45 days after planting (DAP), we determined the plant height (PH), root length (RL), dry matter of the aerial part (DMAP), root dry matter, total dry matter (TDM), relative efficiency (RE), and phosphate utilization efficiency (P-UEF) of the cowpea plants. The Trichoderma isolates showed a greater ability to synthesize IAA and solubilize phosphate than the controls. The best isolates for solubilizing phosphate were UFT 63, UFT 79, UFT 85, and UFT 201. The isolates UFT 25, UFT 79, UFT 110, and UFT 201 were more efficient in synthesizing IAA using tryptophan. The Trichoderma isolates exhibited P-UEF, especially isolates UFT 57, UFT 201, and UFT 204, with values of 430, 282, and 359 %, respectively, compared to controls. Consequently, the isolate UFT 201 (T. asperelloides GJS 04-217) showed greater potential as a growth promoter for cowpea, with a 38 cm PH, 29 cm RL, 4.5 g DMAP, and 5.8 g TDM at 45 DAP and a 256 % phosphorus content and 269 % RE compared to the control without inoculation.
AGRI-ENVIRONMENTAL SCIENCES
Fungos do gênero Trichoderma spp. são capazes de atuar como agentes de controle do crescimento de... more Fungos do gênero Trichoderma spp. são capazes de atuar como agentes de controle do crescimento de fungos patogênicos em várias plantas cultivadas. Diante disso, este trabalho teve como objetivo testar e selecionar isolados de Trichoderma spp. oriundos de áreas do Cerrado tocantinense, para o controle biológico, in vitro, de patógenos de solo da mesma região. Foram coletadas 116 amostras de solos de plantas de feijão e soja, das quais 50 apresentaram isolados de Trichoderma spp. e foram testadas contra os fungos patogênicos Fusarium sp., Rhizoctonia solani e Sclerotium rolfsii. Para testar a ação de Trichoderma spp. sobre esses fitopatógenos, foi utilizada a técnica de pareamento de culturas in vitro com resultados em escala de notas, adaptações e percentagem de colonização. Pela escala de notas, os isolados UFT 06, UFT 28, UFT 63 e UFT 85 foram eficientes antagonistas para todos os fitopatógenos. Na percentagem de inibição do crescimento dos fitopatógenos, 25 isolados com mais de 60...
Brazilian Journal of Development
This work aimed to evaluate the efficiency of the product TrichoPlus (Trichoderma asperellum) as ... more This work aimed to evaluate the efficiency of the product TrichoPlus (Trichoderma asperellum) as a plant growth promoter in soybean and the productive performance at the field in a floodplain in the municipality of Formoso do Araguaia, Tocantins, Brazil. Four treatments with different doses of the TrichoPlus product (2, 4, 6 and 8 g per kg of seeds) were used in the experiment, plus two treatments being a positive control with a commercial product based on Trichoderma asperellum and an absolute control (without inoculation). Biomass, stand and productivity data were evaluated. For the treatment with the TrichoPlus product, a powder formulation was used, with active ingredient based on Trichoderma asperellum 201, formulated with a minimum concentration of 2 x108 UFC g-1, having graphite in the composition as inert, being applied directly to the seeds before planting, aiming better adherence and protection of the Trichoderma in direct contact with the seed and protection right after p...
Revista Brasileira de Biociências, 2016
The use of antagonists in the control of plant pathogens has been extensively studied in recent d... more The use of antagonists in the control of plant pathogens has been extensively studied in recent decades, especially fungi from genus Trichoderma spp., due to their ability to act as biocontrolling agents and inducers of plant resistance against diseases. Thus, we aimed to select Trichoderma spp. with potential to control growth of pathogenic fungi. We used 56 Trichoderma strains from the isolate bank of the microbiology laboratory of Federal University of Tocantins. The strains were isolated from soil samples collected in southern Tocantins state, Brazil. The studied pathogens of foliar diseases were Colletotrichum cliviae and Colletotrichum truncatum, both of which were isolated from soybean plants showing disease symptoms. To evaluate the antagonistic potential of Trichoderma spp., we used the dual culture assay and expressed the results as percentage of colonization and grade scale. Grade evaluation revealed that 46 Trichoderma strains were effective antagonists of the two patho...
Considerando as perdas causadas por doencas em diversas culturas, as rizobacterias se apresentam ... more Considerando as perdas causadas por doencas em diversas culturas, as rizobacterias se apresentam como uma alternativa para manejar esses patogenos. O trabalho teve por finalidade avaliar a potencialidade antagonista de isolados de Bacillus subitilis aos fungos fitopatogenicos Fusarium subglutinans , Curvularia lunata e Bipolaris spp.. Foram estudados sete isolados de B. subtilis e seus efeitos antagonicos em fungos fitopatogenicos utilizando quatro metodos: tecnica de cultura fungica sobre cultura antagonista, pareamento direto, pareamento com risco no centro da placa e tecnica de circulo. Tambem foram avaliados os efeitos de metabolitos volateis e termoestaveis desses isolados. O delineamento experimental utilizado foi inteiramente casualizado atraves de tres repeticoes. As avaliacoes foram feitas aos tres, seis, nove e doze dias para os quatro metodos e aos seis e doze dias para avaliacao de metabolitos volateis e termoestaveis, onde foram medidos o diâmetro da colonia e calculado...
Journal of Scientific Research and Reports, 2021
Trichoderma spp. are fungus considered one of the most attractive for biological control due to i... more Trichoderma spp. are fungus considered one of the most attractive for biological control due to its different mechanisms action against plant pathogens and action to promote plant growth and productivity. The molecular characterization of this group of antagonists is of great importance for conducting studies in the area of biodiversity and for identifying and selecting the possibility of their use in biological control. Objective of this work was to identify 29 Trichoderma isolates based on molecular profiles and phylogenetic analysis sequence the rRNA gene ITS1-5-8S-ITS2, as well as to select the isolates that presented the highest levels of antagonism against S. sclerotiorum and that promoted growth in common bean using experiments in vitro and in a greenhouse. Among the sequences obtained, a total of five different Trichoderma species were identified: T. asperellum, T. harzianum, T. koningiopsis, T. brevicompactum and T. tomentosum. The Trichoderma isolates used showed a variati...
AGRI-ENVIRONMENTAL SCIENCES
Bactérias e fungos são capazes de promover o crescimento vegetal. Dessa forma, o presente trabalh... more Bactérias e fungos são capazes de promover o crescimento vegetal. Dessa forma, o presente trabalho teve como objetivo avaliar o efeito de Bacillus subtilis (UFT BS10) e Trichoderma asperellum (UFT 201), quanto ao incremento de biomassa em plantas de soja, arroz, feijão-caupi e milho em condições de casa de vegetação. Cada cultura foi plantada em vasos utilizando-se solo de área cultivada, caracterizado como Latossolo Vermelho Amarelo Distrófico. Os tratamentos foram inoculações simples e combinadas de B. subtilis e T. asperellum nas sementes e no solo, mais um tratamento testemunha sem inoculação. A estirpe de Bacillus subtilis foi inoculada nas sementes em quantidade de 200 mL para 50 kg de sementes, em concentração de 1 x 108 UFC mL-1. Trichoderma asperelum UFT 201, cultivado em arroz, foi formulado com concentração mínima de 2 x 108 UFC g-1, sendo utilizada a quantidade referente a 2 kg ha-1 do inoculante, misturado ao solo antes do plantio. As avaliações foram aos 20 e 40 dias a...
Symbiosis, Jan 3, 2020
Trichoderma exhibits great ecological and agricultural relevance because it improves plant growth... more Trichoderma exhibits great ecological and agricultural relevance because it improves plant growth, development and productivity. In this study, Trichoderma isolates (T01, T02, T04, T74, T76 and T96 of T. asperelloides; T77 of T. asperellum; or T44, T78 and T92 of T. harzianum) were evaluated in vitro for their ability to solubilize phosphate and produce indole-3-acetic acid (IAA). Afterwards, a completely randomized experimental design with 12 treatments was used to investigate physiological changes in cowpea plants inoculated with Bradyrhizobium sp. BR 3267 (bradyrhizobia) or coinoculated with bradyrhizobia and those Trichoderma isolates in the greenhouse conditions. All Trichoderma isolates showed the ability to solubilize phosphate and to produce IAA. Cowpeas were positively influenced by coinoculation with bradyrhizobia and Trichoderma, with the highlight being cowpea plants coinoculated with bradyrhizobia and T. asperelloides T02. These plants display significant increases in height; relative growth rate; stem diameter; dry weight of shoots, roots and nodules; total dry weight; and specific root length in relation to other symbiotic pairs and absolute control. In contrast, negative responses were registered in cowpea plants coinoculated with bradyrhizobia and T. asperelloides T04, bradyrhizobia and T. harzianum T44 or bradyrhizobia and T. harzianum T92. These plants, together with the absolute control, display lower values of stem diameter, total dry weight, specific root length, and total chlorophyll and carotenoids; the absence of root nodules; and a higher root length and anthocyanin content in relation to other treatments. Our hypothesis is that the increase in root length may be related to IAA produced by Trichoderma, while anthocyanin accumulation is associated with nitrogen deficiency, suggesting that these plants are under stress. To our knowledge, this is the first evidence of antagonistic relationships between bradyrhizobia and Trichoderma in the Highlights • Adequate microbial consortium with Trichoderma may increase legume productivity. • T. asperelloides T02 induces positive responses on cowpea nodulated by bradyrhizobia. • The in vivo synergism among bradyrhizobia and Trichoderma is reported.
Comunicata Scientiae, Apr 6, 2017
The present study aimed to evaluate the Trichoderma asperellum inoculation efficiency in agronomi... more The present study aimed to evaluate the Trichoderma asperellum inoculation efficiency in agronomic performance of soybean plants under field conditions. Two experiments were conducted in a savanna in Gurupi, Tocantins, Brazil, during the 2013/2014 and 2014/2015 crop years, being realized soil chemical analysis and recording the climatic conditions. For the experiment, seeds from the cultivar Monsoy 9144 RR were used and the inoculation with T. asperellum were carried out with a powdered product with active ingredient based on Trichoderma (Trichoderma asperellum-UFT201), formulated with minimum viable concentration of conidia of 2x10 9 g-1 , applied directly on the seeds (20 g/kg seed), in one of the treatments. Were evaluated the biomass, relative efficiency, initial and final stand, effectiveness or efficiency in the use of T. asperellum in maintaining the stand and yield. It was observed that with the use of the treatment with T. asperellum inoculation, the yield of both harvests were 8.5 and 9.1% higher, respectively, when compared to the treatment with no inoculation.
Brazilian Journal of Botany, Jan 9, 2016
The aim of this study was to determine the efficiency of Trichoderma spp. in promoting the growth... more The aim of this study was to determine the efficiency of Trichoderma spp. in promoting the growth of cowpea plants. Strains were isolated from Tocantins savannah soils. Twenty-one isolates were evaluated in vitro for their ability to solubilize calcium phosphate and synthesize indole acetic acid (IAA). The soil samples were characterized physicochemically before planting. Eleven isolates were selected to evaluate the promotion of cowpea plant growth in the greenhouse in soil fertilized with phosphorite in 1.7-L pots. Then, at 32 and 45 days after planting (DAP), we determined the plant height (PH), root length (RL), dry matter of the aerial part (DMAP), root dry matter, total dry matter (TDM), relative efficiency (RE), and phosphate utilization efficiency (P-UEF) of the cowpea plants. The Trichoderma isolates showed a greater ability to synthesize IAA and solubilize phosphate than the controls. The best isolates for solubilizing phosphate were UFT 63, UFT 79, UFT 85, and UFT 201. The isolates UFT 25, UFT 79, UFT 110, and UFT 201 were more efficient in synthesizing IAA using tryptophan. The Trichoderma isolates exhibited P-UEF, especially isolates UFT 57, UFT 201, and UFT 204, with values of 430, 282, and 359 %, respectively, compared to controls. Consequently, the isolate UFT 201 (T. asperelloides GJS 04-217) showed greater potential as a growth promoter for cowpea, with a 38 cm PH, 29 cm RL, 4.5 g DMAP, and 5.8 g TDM at 45 DAP and a 256 % phosphorus content and 269 % RE compared to the control without inoculation.
AGRI-ENVIRONMENTAL SCIENCES
Fungos do gênero Trichoderma spp. são capazes de atuar como agentes de controle do crescimento de... more Fungos do gênero Trichoderma spp. são capazes de atuar como agentes de controle do crescimento de fungos patogênicos em várias plantas cultivadas. Diante disso, este trabalho teve como objetivo testar e selecionar isolados de Trichoderma spp. oriundos de áreas do Cerrado tocantinense, para o controle biológico, in vitro, de patógenos de solo da mesma região. Foram coletadas 116 amostras de solos de plantas de feijão e soja, das quais 50 apresentaram isolados de Trichoderma spp. e foram testadas contra os fungos patogênicos Fusarium sp., Rhizoctonia solani e Sclerotium rolfsii. Para testar a ação de Trichoderma spp. sobre esses fitopatógenos, foi utilizada a técnica de pareamento de culturas in vitro com resultados em escala de notas, adaptações e percentagem de colonização. Pela escala de notas, os isolados UFT 06, UFT 28, UFT 63 e UFT 85 foram eficientes antagonistas para todos os fitopatógenos. Na percentagem de inibição do crescimento dos fitopatógenos, 25 isolados com mais de 60...
Brazilian Journal of Development
This work aimed to evaluate the efficiency of the product TrichoPlus (Trichoderma asperellum) as ... more This work aimed to evaluate the efficiency of the product TrichoPlus (Trichoderma asperellum) as a plant growth promoter in soybean and the productive performance at the field in a floodplain in the municipality of Formoso do Araguaia, Tocantins, Brazil. Four treatments with different doses of the TrichoPlus product (2, 4, 6 and 8 g per kg of seeds) were used in the experiment, plus two treatments being a positive control with a commercial product based on Trichoderma asperellum and an absolute control (without inoculation). Biomass, stand and productivity data were evaluated. For the treatment with the TrichoPlus product, a powder formulation was used, with active ingredient based on Trichoderma asperellum 201, formulated with a minimum concentration of 2 x108 UFC g-1, having graphite in the composition as inert, being applied directly to the seeds before planting, aiming better adherence and protection of the Trichoderma in direct contact with the seed and protection right after p...
Revista Brasileira de Biociências, 2016
The use of antagonists in the control of plant pathogens has been extensively studied in recent d... more The use of antagonists in the control of plant pathogens has been extensively studied in recent decades, especially fungi from genus Trichoderma spp., due to their ability to act as biocontrolling agents and inducers of plant resistance against diseases. Thus, we aimed to select Trichoderma spp. with potential to control growth of pathogenic fungi. We used 56 Trichoderma strains from the isolate bank of the microbiology laboratory of Federal University of Tocantins. The strains were isolated from soil samples collected in southern Tocantins state, Brazil. The studied pathogens of foliar diseases were Colletotrichum cliviae and Colletotrichum truncatum, both of which were isolated from soybean plants showing disease symptoms. To evaluate the antagonistic potential of Trichoderma spp., we used the dual culture assay and expressed the results as percentage of colonization and grade scale. Grade evaluation revealed that 46 Trichoderma strains were effective antagonists of the two patho...
Considerando as perdas causadas por doencas em diversas culturas, as rizobacterias se apresentam ... more Considerando as perdas causadas por doencas em diversas culturas, as rizobacterias se apresentam como uma alternativa para manejar esses patogenos. O trabalho teve por finalidade avaliar a potencialidade antagonista de isolados de Bacillus subitilis aos fungos fitopatogenicos Fusarium subglutinans , Curvularia lunata e Bipolaris spp.. Foram estudados sete isolados de B. subtilis e seus efeitos antagonicos em fungos fitopatogenicos utilizando quatro metodos: tecnica de cultura fungica sobre cultura antagonista, pareamento direto, pareamento com risco no centro da placa e tecnica de circulo. Tambem foram avaliados os efeitos de metabolitos volateis e termoestaveis desses isolados. O delineamento experimental utilizado foi inteiramente casualizado atraves de tres repeticoes. As avaliacoes foram feitas aos tres, seis, nove e doze dias para os quatro metodos e aos seis e doze dias para avaliacao de metabolitos volateis e termoestaveis, onde foram medidos o diâmetro da colonia e calculado...
Journal of Scientific Research and Reports, 2021
Trichoderma spp. are fungus considered one of the most attractive for biological control due to i... more Trichoderma spp. are fungus considered one of the most attractive for biological control due to its different mechanisms action against plant pathogens and action to promote plant growth and productivity. The molecular characterization of this group of antagonists is of great importance for conducting studies in the area of biodiversity and for identifying and selecting the possibility of their use in biological control. Objective of this work was to identify 29 Trichoderma isolates based on molecular profiles and phylogenetic analysis sequence the rRNA gene ITS1-5-8S-ITS2, as well as to select the isolates that presented the highest levels of antagonism against S. sclerotiorum and that promoted growth in common bean using experiments in vitro and in a greenhouse. Among the sequences obtained, a total of five different Trichoderma species were identified: T. asperellum, T. harzianum, T. koningiopsis, T. brevicompactum and T. tomentosum. The Trichoderma isolates used showed a variati...
AGRI-ENVIRONMENTAL SCIENCES
Bactérias e fungos são capazes de promover o crescimento vegetal. Dessa forma, o presente trabalh... more Bactérias e fungos são capazes de promover o crescimento vegetal. Dessa forma, o presente trabalho teve como objetivo avaliar o efeito de Bacillus subtilis (UFT BS10) e Trichoderma asperellum (UFT 201), quanto ao incremento de biomassa em plantas de soja, arroz, feijão-caupi e milho em condições de casa de vegetação. Cada cultura foi plantada em vasos utilizando-se solo de área cultivada, caracterizado como Latossolo Vermelho Amarelo Distrófico. Os tratamentos foram inoculações simples e combinadas de B. subtilis e T. asperellum nas sementes e no solo, mais um tratamento testemunha sem inoculação. A estirpe de Bacillus subtilis foi inoculada nas sementes em quantidade de 200 mL para 50 kg de sementes, em concentração de 1 x 108 UFC mL-1. Trichoderma asperelum UFT 201, cultivado em arroz, foi formulado com concentração mínima de 2 x 108 UFC g-1, sendo utilizada a quantidade referente a 2 kg ha-1 do inoculante, misturado ao solo antes do plantio. As avaliações foram aos 20 e 40 dias a...