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Papers by Mahfuja Begam

Research paper thumbnail of Detection of Metallo b-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in a Tertiary Care Hospital

Sir Salimullah Medical College Journal, Apr 27, 2022

Research paper thumbnail of Comparison of diagnostic efficacy of ultrasound, ultrasound elastography and magnetic resonance imaging for breast lesions

International Journal of Radiology and Diagnostic Imaging, 2021

Background: Rickettsial infections are re-emerging arthropods born worldwide zoonotic disease cau... more Background: Rickettsial infections are re-emerging arthropods born worldwide zoonotic disease caused by Rickettsia, which is responsible for spotted fever and typhus fever. The diagnosis of a rickettsial illness is important for appropriate antibiotic treatment. Aims: The study aimed to determine the diagnostic accuracy and clinical usefulness of using nested polymerase chain reaction (PCR) by comparing nested PCR, ELISA, and Weil-Felix (WF) tests.

Research paper thumbnail of Serological Evidence of Rickettsial Infection by Weil-Felix Test in Tertiary Care Hospital in Bangladesh

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2022

Introduction: Rickettsial infections are re-emerging diseases and are major causes of febrile ill... more Introduction: Rickettsial infections are re-emerging diseases and are major causes of febrile illnesses throughout the Asia-Pacific region. It is difficult to diagnose due to the non-specific clinical manifestations, absence of reliable and affordable diagnostic tests thereby contributes to increasing the acute febrile burden and preventive illness in many populations. Undiagnosed or late-diagnosed cases are associated with high morbidity and mortality. Objectives: The study aimed to determine rickettsial disease by Weil-Felix test and to know the frequency of rickettsial diseases in febrile patients presenting to tertiary care hospitals in Dhaka, Bangladesh. Methods: In this study, a total of 135 peripheral blood samples were taken and tested by Weil Felix test from clinically suspected patients of rickettsial fever. Results: Weil- Felix test was positive in 33((24.4%) cases. Of Weil- Felix test-positive cases, OX-2 was positive in 87.87% cases, followed by OX-K (6.06%), OX-19 (3.0...

Research paper thumbnail of Diagnosis of leprosy by PCR targeting gene encoding 36 kDa antigen of Mycobacterium leprae

Bangladesh Journal of Medical Microbiology

Background & Objective: This cross sectional study was carried out to assess the diagnostic value... more Background & Objective: This cross sectional study was carried out to assess the diagnostic value of PCR in different forms of leprosy. For the detection of Mycobacterium leprae, DNA amplification by polymerase chain reaction of a 531-bp fragment of the Mycobacterium leprae specific gene encoding the 36 kDA antigen. Methodology: It was done on different clinical specimens (slit smear of skin, ear lobule smear and nasal smear) from 50 leprosy patients attending the Leprosy Hospital, Mohakhali, Dhaka. Patients were divided into two groups; paucibacillary (70%) group and multibacillary (30%) group. PCR showed 100% positivity in skin and ear lobule and 73.4% positivity in nasal smear of multibacillary group. PCR was positive in 40%, 25.7% and 11.4% in skin lesion, ear lobule and nasal swab in paucibacillry group respectively. Result: Compared with other diagnostic procedures, PCR showed clear advantages over both modified Z-N stain and auramine-phenol stain especially in paucibacillary ...

Research paper thumbnail of Detection of Metallo b-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in a Tertiary Care Hospital

Sir Salimullah Medical College Journal, Apr 27, 2022

Research paper thumbnail of Comparison of diagnostic efficacy of ultrasound, ultrasound elastography and magnetic resonance imaging for breast lesions

International Journal of Radiology and Diagnostic Imaging, 2021

Background: Rickettsial infections are re-emerging arthropods born worldwide zoonotic disease cau... more Background: Rickettsial infections are re-emerging arthropods born worldwide zoonotic disease caused by Rickettsia, which is responsible for spotted fever and typhus fever. The diagnosis of a rickettsial illness is important for appropriate antibiotic treatment. Aims: The study aimed to determine the diagnostic accuracy and clinical usefulness of using nested polymerase chain reaction (PCR) by comparing nested PCR, ELISA, and Weil-Felix (WF) tests.

Research paper thumbnail of Serological Evidence of Rickettsial Infection by Weil-Felix Test in Tertiary Care Hospital in Bangladesh

Asian Journal of Research in Medical and Pharmaceutical Sciences, 2022

Introduction: Rickettsial infections are re-emerging diseases and are major causes of febrile ill... more Introduction: Rickettsial infections are re-emerging diseases and are major causes of febrile illnesses throughout the Asia-Pacific region. It is difficult to diagnose due to the non-specific clinical manifestations, absence of reliable and affordable diagnostic tests thereby contributes to increasing the acute febrile burden and preventive illness in many populations. Undiagnosed or late-diagnosed cases are associated with high morbidity and mortality. Objectives: The study aimed to determine rickettsial disease by Weil-Felix test and to know the frequency of rickettsial diseases in febrile patients presenting to tertiary care hospitals in Dhaka, Bangladesh. Methods: In this study, a total of 135 peripheral blood samples were taken and tested by Weil Felix test from clinically suspected patients of rickettsial fever. Results: Weil- Felix test was positive in 33((24.4%) cases. Of Weil- Felix test-positive cases, OX-2 was positive in 87.87% cases, followed by OX-K (6.06%), OX-19 (3.0...

Research paper thumbnail of Diagnosis of leprosy by PCR targeting gene encoding 36 kDa antigen of Mycobacterium leprae

Bangladesh Journal of Medical Microbiology

Background & Objective: This cross sectional study was carried out to assess the diagnostic value... more Background & Objective: This cross sectional study was carried out to assess the diagnostic value of PCR in different forms of leprosy. For the detection of Mycobacterium leprae, DNA amplification by polymerase chain reaction of a 531-bp fragment of the Mycobacterium leprae specific gene encoding the 36 kDA antigen. Methodology: It was done on different clinical specimens (slit smear of skin, ear lobule smear and nasal smear) from 50 leprosy patients attending the Leprosy Hospital, Mohakhali, Dhaka. Patients were divided into two groups; paucibacillary (70%) group and multibacillary (30%) group. PCR showed 100% positivity in skin and ear lobule and 73.4% positivity in nasal smear of multibacillary group. PCR was positive in 40%, 25.7% and 11.4% in skin lesion, ear lobule and nasal swab in paucibacillry group respectively. Result: Compared with other diagnostic procedures, PCR showed clear advantages over both modified Z-N stain and auramine-phenol stain especially in paucibacillary ...

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