Mana Oloomi - Academia.edu (original) (raw)

Papers by Mana Oloomi

New Cellular and Molecular Biotechnology Journal, 2016

The Medical Journal of The Islamic Republic of Iran, Feb 10, 1999

The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 2... more The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a(+) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred intoE. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by immunoaffinity chromatography. The dot blot positive fractions were assayed for biological activity and it was shown that the expressed GM-CSF is active.

Cancer reports, Oct 23, 2022

The delay in diagnosis and treatment of breast cancer results in low survival rates and high mort... more The delay in diagnosis and treatment of breast cancer results in low survival rates and high mortality. Thus, it is essential to characterize new therapeutic targets and prognostic breast cancer biomarkers. The rising evidence suggested that long non-coding RNAs (lncRNAs) expression levels are deregulated in human cancers and can use as biomarkers for the rapid diagnosis of breast cancer. In the present study, a Quantitative Real-time polymerase chain reaction (qRT-PCR) technique was used to measure twenty oncogenic and tumor suppressor lncRNAs expression levels in whole blood samples of breast cancer patients and normal controls. Blood samples from 30 healthy women and 30 female breast cancer patients were collected. Then cDNA was synthesized from the extracted RNA blood. The expression level of lncRNAs measured and analyzed by LinReg PCR and REST software and the correla tion between lncRNAs dysregulation and clinical characteristics and prognosis were also analyzed by SPSS software. The comparison between the expression levels of lncRNAs in the blood samples of breast cancer patients compared with healthy individuals revealed that some lncRNAs (MEG3, NBAT1, NKILA, GAS5, EPB41L4A-AS2, ZFAS1, MVIH, Z38, and BC040587) were down regulated. In contrast, other lncRNAs (H19, SPRY4-IT1, UCA1, AC026904.1, CCAT1) were up-regulated, signi cantly. It was shown that the expression levels of NKILA, NBAT1, and ZFAS1 lncRNAs were related to tumor size, and BC040587expression level related to age, node metastasis, tumor size, and grade (P<0.05). The association between H19 and SPRY4-IT1 lncRNAs with HER-2 was con rmed statistically (P<0.05). Our data highlighted the correlation of BC040587, H19, and SPRY4-IT1 lncRNAs with clinicopathological traits in breast cancer patients suggesting their future applications as novel biomarkers and therapeutic targets in breast cancer. In conclusion, circulating lncRNAs could consider as the prognostic and predictive markers in breast cancer.

Journal of medical signals and sensors, 2022

Background: Escherichia coli produces Shiga toxin (Stx), a pentamer composed of one A subunit and... more Background: Escherichia coli produces Shiga toxin (Stx), a pentamer composed of one A subunit and four B subunits. The B subunit of Stx (StxB) mediated the attachment of the holotoxin to the cell surface while the A subunit (StxA) has N-glycosidase activity, resulting in protein synthesis and cell death inhibition. Stx-induced cytotoxicity and apoptosis have been observed in various cell lines, although the signaling effectors are not precisely defined. Activated by protein kinases (PK), the signaling pathway in human tumors plays an oncogenic role. Tumor proliferation, survival, and metastasis are promoted by kinase receptors. In this regard, PK regulatory effects on the cellular constituents of the tumor microenvironment can affect immunosuppressive purposes. Methods: In this study, kinase inhibitors were used to evaluate the influence of Stx and its subunits on HeLa and Vero cells. Selective inhibitors of protein kinase C (PKC), CaM kinase (calmodulin kinase), protein kinase A (PKA), and protein kinase G (PKG) were used to compare the signaling activity of each subunit. Results: The ribotoxic activity in the target cells will lead to rapid protein synthesis inhibition and cell death in the mammalian host. The expression of Bcl2 family members was also assessed. Protein kinase signaling by Stx and its A and B subunits was induced by PKA, PKG, and PKC in HeLa cells. CaM kinase induction was significant in Vero cells. StxB significantly induced the pro-apoptotic Bax signaling factor in HeLa cells. Conclusion: The assessment of different signaling pathways utilized by Stx and its subunits could help in a better understanding of various cell death responses. The use of inhibitors can block cell damage and disease progression and create therapeutic compounds for targeted cancer therapy. Inhibition of these pathways is the primary clinical goal.

Biochemical Society Transactions, Oct 1, 2002

In the present study we have investigated the interaction of AA and PAF in human platelets. We fo... more In the present study we have investigated the interaction of AA and PAF in human platelets. We found that this interaction involves a synergism of a number of signalling mechanisms. Platelet aggregation was studied using human blood. Aggregation was recorded using Dual-channel Chronolog aggregometer. The results demonstrated that AA (0.1-2.0 mM) and PAF (5-800 nM) induced a concentration-dependent increase in aggregation. However, when low doses of AA (0.1-0.2 mM) and PAF (5-40 nM) were added together a marked potentiation of aggregation was observed. This effect was blocked by PAF-receptor antagonist (WEB2086), calcium channel blockers (diltiazem and verapamil), PLC inhibitor (U73122) and a MAP kinase inhibitor (I' D 98059). We also tested the effects of cyclooxygenase inhibitors, indomethacin, flurbiprofen, aspirin, nimesulide and NS-398 which inhibited AA and PAF mediated aggregation with IC50 values of 18, 3, 100, 35 and 28uM respectively. Herbimycin-A, a specific inhibitor of tyrosine kinase also inhibited PAF and AA induced aggregation with an IC50 value of 15uM. Chelerythrine(a PKC inhibitor) had no effect. These data suggest that AA and PAF mediated aggregation involves the activation of multiple signalling pathways.

International Journal of Preventive Medicine, 2020

Background: It has been proven that probiotic Lactobacillus bacteria have inhibitory effects on h... more Background: It has been proven that probiotic Lactobacillus bacteria have inhibitory effects on human cancer cell lines. The aim of this study is to isolate and characterize the antioxidant probiotic Lactobacillus and determine the possible anticancer activities of the selected strain. Methods: One of the Lactobacillus strain isolated from camel doogh sample showed the high antioxidant activity by using of different methods such as resistance to hydrogen peroxide, hydroxyl radical and superoxide anions. The antioxidant strain was characterized by sequencing of 16S rRNA V2-V3 regions and the 16S-23S intergenic spacer (ITS). The methanol extract of this strain supernatant was fractionated using thin layer chromatography (TLC) and antioxidant activity of fractions was detected by 0.1% of DPPH through TLC-DPPH bioautography. In vitro anticancer activity of each fraction was investigated by using MTT and flow cytometry methods. Results: According to the phylogenetic results, the antioxidant Lactobacillus strain was closely related to Lactobacillus hilgardii strain E91 (Accession No. EF536365). After fractionation and anti-proliferation assessments of Lactobacillus hilgardii strain AG12a extracellular materials, one of the antioxidant fraction (F4) showed maximum DPPH radical scavenging activity (IC50 of 535.27 μg/mL). MTT assay of the F4 fraction demonstrated cytotoxic activity against Caco-2 with the IC50 value of 299.05 μg/mL. The cell death activity of the fraction was confirmed by flow cytometry with 30.925. Conclusions: In this study, the anticancer and apoptotic properties of Lactobacillus hilgardii against Caco-2 cell line was reported for the first time. The isolated bioactive fraction from the extracellular methanol extract needs to be further investigated in human studies of cancer therapy.

PubMed, Mar 1, 2010

Background and objectives: Enteropathogenic Escherichia coli (EPEC) strains can be detected by se... more Background and objectives: Enteropathogenic Escherichia coli (EPEC) strains can be detected by serogrouping and the presence of enterocyte attaching- effacing (eae) gene. Most EPEC strains belong to a certain O antigenic group. Locus of enterocyte effacement (LEE) Pathogenicity Island contains the eae gene and secretory proteins (ESPs) that introduce the attaching-effacing lesion. LEE inserted in tRNA genes include the SelC, PheU and PheV sites. The aim of the present study was to genetically characterize EPEC strains isolated from children with diarrhea. Materials and methods: Serogrouping was performed by EPEC antisera in 321 E. coli isolates. The presence of eae, stx, espB, and eaf genes and detection of insertion sites of LEE was done by PCR using specific primers. Results: Seventeen (5.3%) isolates belonging to 7 EPEC serogroups were identified among the whole material and all carried the eae gene. None of the 321 isolates showed presence of stx gene indicating that all 17 isolates classified as EPEC by O serogrouping did not belong to the enterohaemorrhagic E. coli (EHEC) group. Of these, 8 (53%) isolates carried the eaf and 16 (94.1%) carried the espB gene. The insertion sites of LEE in serogrouped isolates were selC (in 6 isolates), pheU (in 7 isolates) and pheV (in 2 isolates). The insertion site in 2 isolates was not determined by PCR. Conclusion: Serogrouping and detection of the eae gene showed to be reliable for detection of EPEC strains. No Shigatoxin- producing E. coli (STEC) strain was found among the isolates. Detection of the insertion site of LEE showed that selC, pheU or PheV are insertion sites of LEE in the EPEC strains.

Jundishapur Journal of Microbiology, Sep 8, 2015

Background: Several studies performed in developed and developing countries have identified enter... more Background: Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea. Objectives: This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children with diarrhea between 2007-2008 in Tehran, Iran. Materials and Methods: EAEC strains were examined for virulence plasmid genes (aap, aggR, and aatA), biofilm formation, and drug resistance. In addition, pulsed-field gel electrophoresis (PFGE) profiles of these strains were determined. Results: Significant percentage of local EAEC strains carried the virulence plasmid genes and formed biofilms. In addition, these strains showed high resistance to ampicillin (100%), tetracycline (65.7%), streptomycin (58.7%), chloramphenicol (52.6%), and trimethoprim/ sulfamethoxazole (51.7%) and had different PFGE patterns. Conclusions: These results indicated that EAEC strains isolated from Iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries.

Probiotics and Antimicrobial Proteins

Frontiers in Cellular and Infection Microbiology

Hypervirulent Klebsiella pneumoniae (hvKp) pathotype is emerging worldwide in pyogenic liver absc... more Hypervirulent Klebsiella pneumoniae (hvKp) pathotype is emerging worldwide in pyogenic liver abscesses (PLAs). However, the role of virulence factors in pathogenicity remains unclear. On the other hand, the epidemiology of PLAs in Iran is unknown. From July 2020 to April 2022, bacterial species were isolated and identified from the drainage samples of 54 patients with PLAs. K. pneumoniae as the most common pathogen of pyogenic liver abscesses was identified in 20 (37%) of the 54 patients. We analyzed the clinical and microbiological characteristics of K. pneumoniae-related pyogenic liver abscesses. Antibiotic susceptibility testes and string test were performed. 16S rRNA, antibiotic resistance, and virulence genes were determined by polymerase chain reaction amplification. Clonal relatedness of isolates was identified by multilocus sequence typing. Virulence levels were assessed in the Galleria mellonella larval infection model. Four hvKp isolates (K1/K2) were found to be responsibl...

Journal of Biomolecular Structure and Dynamics, 2020

The development of bacterial resistance toward antibiotics has been led to pay attention to the a... more The development of bacterial resistance toward antibiotics has been led to pay attention to the antimicrobial peptides (AMPs). The common mechanism of AMPs is disrupting the integrity of the bacterial membrane. One of the most accessible targets for a-defensins human neutrophil peptide-1 (HNP-1) is lipid II. In the present study, we performed homology modeling and geometrical validation of human neutrophil defensin 1. Then, the conformational and physicochemical properties of HNP-1 derived peptides 2Abz 14 S 29 , 2Abz 23 S 29 , and HNP1DC18A, as well as their interaction with lipid II were studied computationally. The overall quality of the predicted model of full protein was À5.14, where over 90% of residues were in the most favored and allowed regions in the Ramachandran plot. Although HNP-1 and HNP1DC18A were classified as unstable peptides, 2Abz 14 S 29 and 2Abz 23 S 29 were stable, based on the instability index values. Molecular docking showed similar interaction pattern of peptides and HNP-1 to lipid II. Molecular dynamic simulations revealed the overall stability of conformations, though the fluctuations of amino acids in the modified peptides were relatively higher than HNP-1. Further, the binding affinity constant (Kd) of HNP-1 and 2Abz 23 S 29 in complex with lipid II was 10 times stronger than 2Abz 14 S 29 and HNP1DC18A. Overall, computational studies of conformational and interaction patterns have signified how derived peptides could have displayed relatively similar antimicrobial results compared to HNP-1 in the reported experimental studies. Chemical modifications not only have improved the physicochemical properties of derived peptides compared to HNP-1, but also they have retained the similar pattern and binding affinity of peptides.

Rising evidence proved the existence of dormant blood microbiota in healthy individuals, yet ther... more Rising evidence proved the existence of dormant blood microbiota in healthy individuals, yet there is no information on the circulating microbiome of Iranian healthy women. The High-throughput Next-Generation Sequencing (NGS) provides a powerful method for the characterization of the blood microbiome. We investigated circulating bacterial composition in healthy individuals by 16S rDNA-based next-generation sequencing technique targeting the V3-V4 hypervariable region on the Illumina platform. We identi ed Operational Taxonomic Units (OTUs) similarity with various phyla, predominated by Proteobacteria (83%), Firmicutes (8%), Bacteroidetes (6%), and Actinobacteria (2%) phyla in healthy subjects. Based on the sequencing data, a diverse bacterial family was found in the whole blood of the healthy subjects, belongs to Burkholderiaceae (69%) and Enterobacteriaceae (13%) families on average. At the genus level, we illustrated the abundance of Ralstonia, Rhizobium, Ignatzchineria, Lactococcus, Stenotrophomonas, and Ideonella. Given the dominance of the putative blood microbiota within the different niches, it may be of considerable clinical signi cance and therapeutic strategies potential in the future. The blood microbiota can facilitate both the diagnosis and improved understanding of the onset of numerous human diseases as innovative biomarkers.

Iranian biomedical journal, Nov 15, 2010

Recombinant hybrid protein, Shiga toxin and granulocyte macrophage colony stimulating factor effe... more Recombinant hybrid protein, Shiga toxin and granulocyte macrophage colony stimulating factor effectively induce apoptosis of colon cancer cells

Journal of Medicine and Life

Cytokeratin19 (CK19) was detected as the most related marker for circulating tumor cells, which w... more Cytokeratin19 (CK19) was detected as the most related marker for circulating tumor cells, which was assessed in specific cell lines. MCF7, SKBR3, T47D, and MDA-MB-231, and HeLa cell line as negative control were used. CK19 expression was confirmed by using mouse monoclonal anti-human CK19 antibody. CK19 detection in MDA-MB-231 was not observed. CK19 marker expression was compared in T47D, MCF7, and SKBR3 cell lines. T47D and MCF7 belonged to the luminal subtype of breast cancer (BC) that CK19 expression regulated with an ER marker. SKBR3 belonged to the HER2 positive subtype of BC. However, MDA-MB-231 belonged to the claudin-low subtype of BC that lack of CK19 expression strongly is related to negative ER, PR, and HER2. Therefore, there are not only quantitative differences in CK19 expression, but its expression could also link to the other markers of BC that should be considered in the molecular classification of breast carcinoma. Different expression levels related to cell classif...

Iranian Biomedical Journal

Background: The presence of microbiome in the blood samples of healthy individuals has been addre... more Background: The presence of microbiome in the blood samples of healthy individuals has been addressed. However, no information can be found on the healthy human blood microbiome of Iranian subjects. The current study is thus aimed to investigate the existence of bacteria or bacterial DNA in healthy individuals. Methods: Blood samples of healthy subjects were incubated in BHI broth at 37 °C for 72 h. The 16S rRNA PCR and sequencing were performed to analyze bacterial isolates. The 16S rRNA PCR was directly carried out on DNA samples extracted from the blood of healthy individuals. NGS was conducted on blood samples with culture-positive results. Results: Fifty blood samples were tested, and six samples were positive by culture as confirmed by Gram staining and microscopy. The obtained 16S rRNA sequences of cultured bacterial isolates revealed the presence of Bacilli and Staphylococcus species by clustering in the GeneBank database (≥97% identity). The 16S rRNA gene sequencing results of one non-cultured blood specimen showed the presence of Burkholderia. NGS results illustrated the presence of Romboutsia, Lactobacillus, Streptococcus, Bacteroides, and Staphylococcus in the blood samples of positive cultures. Conclusion: The dormant blood microbiome of healthy individuals may give the idea that the steady transfer of bacteria into the blood does not necessarily lead to sepsis. However, the origins and identities of bloodassociated bacterial rDNA sequences need more evaluation in the healthy population.