Manal abdel-rahman - Academia.edu (original) (raw)

Uploads

Papers by Manal abdel-rahman

Scientific Journal of Flowers and Ornamental Plants, 2020

Ocimum basilicum L. sweet basil is native to India and Iran, an aromatic herb and perennial belon... more Ocimum basilicum L. sweet basil is native to India and Iran, an aromatic herb and perennial belong to the Lamiaceae family. This study was done at the Genetic Engineering and Biotechnology Laboratory, Faculty of Agriculture, Damanhour University, Egypt from 2019 to 2020. Developing stress tolerant plants using in vitro selection is an effective method; therefore, the purpose of this study was to detect the best concentration of phytohormones to produce embryogenic callus of Ocimum basilicum L. and study the effect of poly ethylene glycol (PEG) on embryogenic callus of basil. Leaf specimens of basil were cultured on MS medium (Murashige and Skoog) with concentration of auxin and cytokinin. A1 (2, 4-D at 0.5 mg/l), A2 (2, 4-D at 1.0 mg/l) and A3 (2, 4-D and BAP at 1.0 mg/l and 0.2 mg/l). Embryogenic callus of basil was sub cultured under normal and drought stress media containing different concentration of polyethylene glycol (PEG; M.W 4000) at 2, 5 and 10%. Results showed that medium A3 was the best medium of embryogenic callus induction. Also, the concentration of PEG at 5% was shown maximized callus survivability compared with the other PEG concentrations with no significant effect. This study adds new information on the effect of 5% PEG which showed the highest (positive) survivability of callus. The morphogenic of calluses on 5% PEG were yellowish, friable while the morphogenic of calluses on 10% PEG turned into brown.

Egyptian Journal of Botany, 2021

Silicon (Si) is beneficial for plant growth and has the potential to alleviate the deleterious ef... more Silicon (Si) is beneficial for plant growth and has the potential to alleviate the deleterious effects of heavy metals in plants grown on contaminated soils. This study aimed to evaluate the adaptive mechanisms induced by Si application (1 mM sodium meta-silicate, Na2O3Si.9H2Ox) in Triticum aestivum L. plants subjected to cadmium (Cd) stress (100 and 200 µM CdSO4). Under Cd stress, Si application significantly increased plant biomass, relative water content, nutrient uptake and allocation as well as Si content while it decreased Cd accumulation compared to Cd-stressed plants. Si application also induced lignin content, mainly in roots, in presence or absence of Cd in comparison to controls. Cd stress significantly increased the accumulation of oxalate, malate and citrate contents in the roots in comparison to control, whereas Si supplementation increased malate and citrate in shoots. Additionally, Cd-induced oxidative stress designated by increment of malondialdehyde, H2O2 contents ...

Tissue and cell culture systems are vital to manyareas of maize research and improvement. In the ... more Tissue and cell culture systems are vital to manyareas of maize research and improvement. In the present research two inbred lines i.e. SD7 and SD34 were used to obtian callus induction. The highest values for alive callus were 260 and 180 in SD7 and 34, respectively after six selections, while, the lowest values were 137 and 120 in respect. The overall of alive callus was 190 and 148 and dead callus was 76 and 2.17 for SD7 and 34.The percentage of dead callus was 2% compared with the other variety was 40%.

Maize is one of the main food crops worldwide. In this work two single cross varieties 168and SC1... more Maize is one of the main food crops worldwide. In this work two single cross varieties 168and SC10 were used to get friable callus. Results showed that variety 168 is more efficient than SC10 in callus regeneration and response for sub-culture. The higher alive calluses were 88.17 and 41.0 in 168 and SC10 varieties, respectively. The number of dead calluses in variety SC10 was 26.67 compared with variety 168 was 7.17. The main conclusions from these results that variety 168 could be used for transformation more efficiency SC10 based on their callus morphological characteristics.

In this study, many findings were observed by monitoring seasonal variations of physical, chemica... more In this study, many findings were observed by monitoring seasonal variations of physical, chemical and microbiological characteristics of raw water of seven main canals used by Water Purification Plants in Kafr El-Sheikh governorate; those findings elucidated the quality of these resources and how would they become affected by pollutants especially during winter season and low demand period of Nile River. This study discussed nitrification process occurrence and how would this process and those characteristics affect each other. These seasonal changes during winter season are affecting and altering physicochemical and microbiological parameters of raw water in a bad way causing many problems especially in drinking water purification plants located on Nile River and main canals. Identification of ammonia oxidation and degradation responsible bacteria revealed that Nitrosomonas europaea and Bacillus amyloliquefaciens were involved in ammonia removal and nitrification process.

American Journal of Bioscience and Bioengineering, 2019

The agricultural plans in Egypt aim to increase maize production and yield to reduce the importat... more The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.

Biofuels, 2016

ABSTRACT Lignin is an aromatic biopolymer, an integral cell wall constituent in all vascular plan... more ABSTRACT Lignin is an aromatic biopolymer, an integral cell wall constituent in all vascular plants including the herbaceous varies. Genetic engineering using an antisense system offers the way to modulate enzymes in the lignin pathway and leads to reduced lignin content. The Hi II, a highly embryogenic genotype of maize, has been extensively used in transformation protocols. The plasmid pZMAS-C4H3 10,907bp has C4H3 antisense (AS) to down regulate O-methyltransferase and reduce lignin level in the maize and bar gene as selectable marker that confers glufosinate herbicide resistance under 35s promoter. Eleven transgenic lines exhibited detectable levels of bar gene activity. Three lines of AS plants, C4H3-AS, displayed a significant reduction in lignin content (14–17%). The results of this study pointed to possible use of the genetic transformation approach to minimize lignin content and consequently enhancing biofuel production from stover.

In Vitro Cellular & Developmental Biology - Plant, 2010

Type II maize callus of the HiII genotype can be separated into regenerable and non-regenerable t... more Type II maize callus of the HiII genotype can be separated into regenerable and non-regenerable types based on the visible morphology of the callus. When the non-regenerable morphotype of callus initiated 6 mo or a yr earlier was treated with from 2% to 20% polyethylene glycol (PEG; 3,350 molecular weight) for three subculture periods of 21 d each, the morphotype changed to

Phytochemistry, 2012

Metabolic changes were studied, which accompanied the conversion of 6 month old HiII maize nonreg... more Metabolic changes were studied, which accompanied the conversion of 6 month old HiII maize nonregenerable (NR) calli into regenerable (R) calli when cultured for 63 days with 10% polyethylene glycol (PEG) (3350 MW) in culture medium. The conversion of 6 month old NR to R callus morphotype caused by PEG application decreased cell wall contents in callus dry mass and changed cell wall phenolics making their profile similar to that of R callus by reduction of lignin and ester-and ether-bound phenolic concentrations, including p-coumaric acid and ester-and ether-bound diferulates and by increase of the ratios of ester-and ether-bound ferulic acid/coumaric acid and ferulic acid/diferulic acid in cell walls of NR callus. Some similar changes of cell wall phenolics caused by PEG application were also found in 48 month old NR callus, that changed the morphology, but did not regenerate plants. However, there were no changes in the old callus in levels of total ester and ether-bound cell wall phenolics and substantially smaller decreases were found in ratios of ester-and ether-bound ferulic acid/coumaric acid and ferulic acid/diferulic acid, as well as in diferulate concentrations compared to young NR callus cultured with PEG. Remarkably, application of PEG also changed the primary metabolism of young NR callus tissues, so that they acquired metabolic features of highly regenerable callus. These data clearly suggest that PEG alters metabolism of NR calli, so they acquire biochemical characteristics of R calli, and that adaptive osmotic adjustments vary in different types of callus tissues.

Asian Journal of Poultry Science, Apr 1, 2015

The present study aimed to measure the direct response of selection for high egg weight of the fi... more The present study aimed to measure the direct response of selection for high egg weight of the first 10 eggs laid at the beginning of egg production period in Japanese quail through three generations of selection. The egg weight of the first 10 eggs (EW 10) as direct response increased in the selected line when compared with control line from 10.43 g in the base generation to 12.52 g in the second generation after selection with rate of 0.69 g per generation. The Age at Sexual Maturity (ASM) trait increased in the selected line from 49.47 days in the base generation to 52.63 days in the second generation after selection for EW 10 with rate of 1.05 days per generation. The Body Weight at Sexual Maturity (BWSM) trait increased, after selection in the selected line from 240.09 g in the base generation to 251.45 and 243.67 g in the first and second generation, respectively. Egg number during the first 45 days of production was significantly higher in selected line than the control line throughout the 3 generations of selection. The overall mean of egg weight through the first 45 days of laying period were11.27, 12.01 and 12.29 g for the base, 1st and 2nd generation while it was 11.51 and 12.13 g for control and selected lines, respectively, with a significant difference among generations and between line. Average feed consumption was 31.50 and 29.03 g/female/day for the control and selected line, respectively. The females in selected line had better Feed Conversion Ratio (FCR) than the control females. Selection for EW 10 in Japanese quail improved egg number, egg weight and FCR during the first 45 days of laying.

Molecular analysis is an easier means to identify and isolate a specific gene which has imperativ... more Molecular analysis is an easier means to identify and isolate a specific gene which has imperative function for growth, body composition , fat deposition, metabolic and skeletal traits as well as the molecular genetics selection on individual genes is a very efficient method to genetically improve economically important traits in chickens. Insulin- like growth factor 1 ( IGF-1)is a member of a heterogeneous group of peptides with important growth.Transforming growth factorβ ( TGF-β) belongs to a large family of growth and differentiation factors that play a pivotal role in a great variety of biological activities including morphogenesis, development and differentiation. DNA was extracted from 48 chickens sampled from three strains Lohman (17) , Sinai (24) and Gimmizah (7) IGF-1 gene and TGFβ2 gene were amplified using PCR protocol. Electrophoresis was carried out on the products of PCR , bands viewed on transilluminator. The size of IGF-1 gene was 675 bp while the size of TGFβ2 gene...

Scientific Journal of Flowers and Ornamental Plants, 2020

Ocimum basilicum L. sweet basil is native to India and Iran, an aromatic herb and perennial belon... more Ocimum basilicum L. sweet basil is native to India and Iran, an aromatic herb and perennial belong to the Lamiaceae family. This study was done at the Genetic Engineering and Biotechnology Laboratory, Faculty of Agriculture, Damanhour University, Egypt from 2019 to 2020. Developing stress tolerant plants using in vitro selection is an effective method; therefore, the purpose of this study was to detect the best concentration of phytohormones to produce embryogenic callus of Ocimum basilicum L. and study the effect of poly ethylene glycol (PEG) on embryogenic callus of basil. Leaf specimens of basil were cultured on MS medium (Murashige and Skoog) with concentration of auxin and cytokinin. A1 (2, 4-D at 0.5 mg/l), A2 (2, 4-D at 1.0 mg/l) and A3 (2, 4-D and BAP at 1.0 mg/l and 0.2 mg/l). Embryogenic callus of basil was sub cultured under normal and drought stress media containing different concentration of polyethylene glycol (PEG; M.W 4000) at 2, 5 and 10%. Results showed that medium A3 was the best medium of embryogenic callus induction. Also, the concentration of PEG at 5% was shown maximized callus survivability compared with the other PEG concentrations with no significant effect. This study adds new information on the effect of 5% PEG which showed the highest (positive) survivability of callus. The morphogenic of calluses on 5% PEG were yellowish, friable while the morphogenic of calluses on 10% PEG turned into brown.

Egyptian Journal of Botany, 2021

Silicon (Si) is beneficial for plant growth and has the potential to alleviate the deleterious ef... more Silicon (Si) is beneficial for plant growth and has the potential to alleviate the deleterious effects of heavy metals in plants grown on contaminated soils. This study aimed to evaluate the adaptive mechanisms induced by Si application (1 mM sodium meta-silicate, Na2O3Si.9H2Ox) in Triticum aestivum L. plants subjected to cadmium (Cd) stress (100 and 200 µM CdSO4). Under Cd stress, Si application significantly increased plant biomass, relative water content, nutrient uptake and allocation as well as Si content while it decreased Cd accumulation compared to Cd-stressed plants. Si application also induced lignin content, mainly in roots, in presence or absence of Cd in comparison to controls. Cd stress significantly increased the accumulation of oxalate, malate and citrate contents in the roots in comparison to control, whereas Si supplementation increased malate and citrate in shoots. Additionally, Cd-induced oxidative stress designated by increment of malondialdehyde, H2O2 contents ...

Tissue and cell culture systems are vital to manyareas of maize research and improvement. In the ... more Tissue and cell culture systems are vital to manyareas of maize research and improvement. In the present research two inbred lines i.e. SD7 and SD34 were used to obtian callus induction. The highest values for alive callus were 260 and 180 in SD7 and 34, respectively after six selections, while, the lowest values were 137 and 120 in respect. The overall of alive callus was 190 and 148 and dead callus was 76 and 2.17 for SD7 and 34.The percentage of dead callus was 2% compared with the other variety was 40%.

Maize is one of the main food crops worldwide. In this work two single cross varieties 168and SC1... more Maize is one of the main food crops worldwide. In this work two single cross varieties 168and SC10 were used to get friable callus. Results showed that variety 168 is more efficient than SC10 in callus regeneration and response for sub-culture. The higher alive calluses were 88.17 and 41.0 in 168 and SC10 varieties, respectively. The number of dead calluses in variety SC10 was 26.67 compared with variety 168 was 7.17. The main conclusions from these results that variety 168 could be used for transformation more efficiency SC10 based on their callus morphological characteristics.

In this study, many findings were observed by monitoring seasonal variations of physical, chemica... more In this study, many findings were observed by monitoring seasonal variations of physical, chemical and microbiological characteristics of raw water of seven main canals used by Water Purification Plants in Kafr El-Sheikh governorate; those findings elucidated the quality of these resources and how would they become affected by pollutants especially during winter season and low demand period of Nile River. This study discussed nitrification process occurrence and how would this process and those characteristics affect each other. These seasonal changes during winter season are affecting and altering physicochemical and microbiological parameters of raw water in a bad way causing many problems especially in drinking water purification plants located on Nile River and main canals. Identification of ammonia oxidation and degradation responsible bacteria revealed that Nitrosomonas europaea and Bacillus amyloliquefaciens were involved in ammonia removal and nitrification process.

American Journal of Bioscience and Bioengineering, 2019

The agricultural plans in Egypt aim to increase maize production and yield to reduce the importat... more The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.

Biofuels, 2016

ABSTRACT Lignin is an aromatic biopolymer, an integral cell wall constituent in all vascular plan... more ABSTRACT Lignin is an aromatic biopolymer, an integral cell wall constituent in all vascular plants including the herbaceous varies. Genetic engineering using an antisense system offers the way to modulate enzymes in the lignin pathway and leads to reduced lignin content. The Hi II, a highly embryogenic genotype of maize, has been extensively used in transformation protocols. The plasmid pZMAS-C4H3 10,907bp has C4H3 antisense (AS) to down regulate O-methyltransferase and reduce lignin level in the maize and bar gene as selectable marker that confers glufosinate herbicide resistance under 35s promoter. Eleven transgenic lines exhibited detectable levels of bar gene activity. Three lines of AS plants, C4H3-AS, displayed a significant reduction in lignin content (14–17%). The results of this study pointed to possible use of the genetic transformation approach to minimize lignin content and consequently enhancing biofuel production from stover.

In Vitro Cellular & Developmental Biology - Plant, 2010

Type II maize callus of the HiII genotype can be separated into regenerable and non-regenerable t... more Type II maize callus of the HiII genotype can be separated into regenerable and non-regenerable types based on the visible morphology of the callus. When the non-regenerable morphotype of callus initiated 6 mo or a yr earlier was treated with from 2% to 20% polyethylene glycol (PEG; 3,350 molecular weight) for three subculture periods of 21 d each, the morphotype changed to

Phytochemistry, 2012

Metabolic changes were studied, which accompanied the conversion of 6 month old HiII maize nonreg... more Metabolic changes were studied, which accompanied the conversion of 6 month old HiII maize nonregenerable (NR) calli into regenerable (R) calli when cultured for 63 days with 10% polyethylene glycol (PEG) (3350 MW) in culture medium. The conversion of 6 month old NR to R callus morphotype caused by PEG application decreased cell wall contents in callus dry mass and changed cell wall phenolics making their profile similar to that of R callus by reduction of lignin and ester-and ether-bound phenolic concentrations, including p-coumaric acid and ester-and ether-bound diferulates and by increase of the ratios of ester-and ether-bound ferulic acid/coumaric acid and ferulic acid/diferulic acid in cell walls of NR callus. Some similar changes of cell wall phenolics caused by PEG application were also found in 48 month old NR callus, that changed the morphology, but did not regenerate plants. However, there were no changes in the old callus in levels of total ester and ether-bound cell wall phenolics and substantially smaller decreases were found in ratios of ester-and ether-bound ferulic acid/coumaric acid and ferulic acid/diferulic acid, as well as in diferulate concentrations compared to young NR callus cultured with PEG. Remarkably, application of PEG also changed the primary metabolism of young NR callus tissues, so that they acquired metabolic features of highly regenerable callus. These data clearly suggest that PEG alters metabolism of NR calli, so they acquire biochemical characteristics of R calli, and that adaptive osmotic adjustments vary in different types of callus tissues.

Asian Journal of Poultry Science, Apr 1, 2015

The present study aimed to measure the direct response of selection for high egg weight of the fi... more The present study aimed to measure the direct response of selection for high egg weight of the first 10 eggs laid at the beginning of egg production period in Japanese quail through three generations of selection. The egg weight of the first 10 eggs (EW 10) as direct response increased in the selected line when compared with control line from 10.43 g in the base generation to 12.52 g in the second generation after selection with rate of 0.69 g per generation. The Age at Sexual Maturity (ASM) trait increased in the selected line from 49.47 days in the base generation to 52.63 days in the second generation after selection for EW 10 with rate of 1.05 days per generation. The Body Weight at Sexual Maturity (BWSM) trait increased, after selection in the selected line from 240.09 g in the base generation to 251.45 and 243.67 g in the first and second generation, respectively. Egg number during the first 45 days of production was significantly higher in selected line than the control line throughout the 3 generations of selection. The overall mean of egg weight through the first 45 days of laying period were11.27, 12.01 and 12.29 g for the base, 1st and 2nd generation while it was 11.51 and 12.13 g for control and selected lines, respectively, with a significant difference among generations and between line. Average feed consumption was 31.50 and 29.03 g/female/day for the control and selected line, respectively. The females in selected line had better Feed Conversion Ratio (FCR) than the control females. Selection for EW 10 in Japanese quail improved egg number, egg weight and FCR during the first 45 days of laying.

Molecular analysis is an easier means to identify and isolate a specific gene which has imperativ... more Molecular analysis is an easier means to identify and isolate a specific gene which has imperative function for growth, body composition , fat deposition, metabolic and skeletal traits as well as the molecular genetics selection on individual genes is a very efficient method to genetically improve economically important traits in chickens. Insulin- like growth factor 1 ( IGF-1)is a member of a heterogeneous group of peptides with important growth.Transforming growth factorβ ( TGF-β) belongs to a large family of growth and differentiation factors that play a pivotal role in a great variety of biological activities including morphogenesis, development and differentiation. DNA was extracted from 48 chickens sampled from three strains Lohman (17) , Sinai (24) and Gimmizah (7) IGF-1 gene and TGFβ2 gene were amplified using PCR protocol. Electrophoresis was carried out on the products of PCR , bands viewed on transilluminator. The size of IGF-1 gene was 675 bp while the size of TGFβ2 gene...