Nizar Abd Manan - Academia.edu (original) (raw)

Uploads

Papers by Nizar Abd Manan

Research paper thumbnail of Low dose of tocotrienols protects osteoblasts against oxidative stress

La Clinica terapeutica, 2011

Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. ... more Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of a-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Toxicity tests were carried out on hydrogen peroxide (H(2)O(2)), a-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC(50)) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of a-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC50 of H(2)O(2) for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H(2)O(2). The IC(50) after 2 hours and 24 hours incubation time for H(2)O(2) were 490 µM and 280 µM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC(50) of 290 µM after 24 hours incubation time while a-tocopherol was not ...

Research paper thumbnail of Inhibitory and Apoptosis-Inducing Effects of Newcastle Disease Virus Strain AF2240 on Mammary Carcinoma Cell Line

BioMed Research International, 2015

Breast cancer is the malignant tumour that developed from cells of the breast and is the first le... more Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women worldwide. Surgery, radiotherapy, and chemotherapy are the available treatments for breast cancer, but these were reported to have side effects. Newcastle disease virus (NDV) known as Avian paramyxovirus type-1 (APMV1) belongs to the genus Avulavirus in a family Paramyxoviridae. NDV is shown to be a promising anticancer agent, killing tumour cells while sparing normal cells unharmed. In this study, the oncolytic and cytotoxic activities of NDV AF2240 strain were evaluated on MDA-MB-231, human mammary carcinoma cell line, using MTT assay, and its inhibitory effects were further studied using proliferation and migration assays. Morphological and apoptotic-inducing effects of NDV on MD-MB-231 cells were observed using phase contrast and fluorescence microscopes. Detection of DNA fragmentation was done following terminal deoxyribonucleotide transferasemediated Br-dUTP nick end labeling staining (TUNEL) assay, which confirmed that the mode of death was through apoptosis and was quantified by flow cytometry. Furthermore, analysis of cellular DNA content demonstrated that the virus caused an increase in the sub-G1 phase (apoptotic peak) of the cell cycle. It appears that NDV AF2240 strain is a potent anticancer agent that induced apoptosis in time-dependent manner.

Research paper thumbnail of Detection and Quantification of Fragmented Dna in Breast Cancer Cell Line Treated with Newcastle Disease Virus (NDV)

Introduction: Breast cancer is the malignant tumour that developed from cells of the breast. This... more Introduction: Breast cancer is the malignant tumour that developed from cells of the breast. This disease, is the first leading cause of cancer death among women globally and the most prevalent cancer in the world. Surgery, radiotherapy, and chemotherapy are the available treatment for breast cancer, but these were reported to have some side effects. Newcastle disease virus (NDV), also known as avian paramyxovirus type-1, APMV-1 is belonging to the genus Avulavirus in a family Paramyxoviridae. The virus is of economic important in that, it result in significant loss to the poultry industry, due to its effect on their brain, gastrointestinal and respiratory tracts. However, exposure to human results in a mild conjunctivitis, laryngitis and influenza-like symptoms. NDV showed promising as an anti-cancer agent as it kill tumour cells while leaving the normal cells unharm. Several strains of NDV such as 73-T, MTH68 and HUJ have been shown to posses the ability to kill cancer cells. Addi...

Research paper thumbnail of Umar et al Poster NPS-UPM

Breast cancer is the malignant tumour that developed from cells of the breast and is the first le... more Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women globally. Surgery, radiotherapy, and chemotherapy are the available treatment for breast cancer, but these were reported to have some side effects. Newcastle disease virus (NDV), also known as avian paramyxovirus type-1,is belong to the genus Avulavirus in a family Paramyxoviridae. The virus is of economic important to the poultry industry as it results on its significant damages. However, exposure to human results in a mild conjunctivitis, laryngitis and influenza-like symptoms. NDV showed promising as anti-cancer agent, killing tumour cells while sparing normal cells unharm. Several strains of NDV such as 73-T, MTH68 and HUJ have been shown to posses the ability to kill cancer cells. Additionally, three (3) Malaysian strains, AF2240, S and V4-UPM, have also been studied on different cancer cell lines. Therefore, our objective was to investigate w...

Research paper thumbnail of Effects of Low-Dose versus High-Dose γ-Tocotrienol on the Bone Cells Exposed to the Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis

Evidence-Based Complementary and Alternative Medicine, 2012

Oxidative stress and apoptosis can disrupt the bone formation activity of osteoblasts which can l... more Oxidative stress and apoptosis can disrupt the bone formation activity of osteoblasts which can lead to osteoporosis. This study was conducted to investigate the effects of γ-tocotrienol on lipid peroxidation, antioxidant enzymes activities, and apoptosis of osteoblast exposed to hydrogen peroxide (H 2 O 2 ). Osteoblasts were treated with 1, 10, and 100 μM of γ-tocotrienol for 24 hours before being exposed to 490 μM (IC 50 ) H 2 O 2 for 2 hours. Results showed that γ-tocotrienol prevented the malondialdehyde (MDA) elevation induced by H 2 O 2 in a dose-dependent manner. As for the antioxidant enzymes assays, all doses of γ-tocotrienol were able to prevent the reduction in SOD and CAT activities, but only the dose of 1 μM of GTT was able to prevent the reduction in GPx. As for the apoptosis assays, γ-tocotrienol was able to reduce apoptosis at the dose of 1 and 10 μM. However, the dose of 100 μM of γ-tocotrienol induced an even higher apoptosis than H 2 O 2 . In conclusion, low doses of γ-tocotrienol offered protection for osteoblasts against H 2 O 2 toxicity, but itself caused toxicity at the high doses.

Research paper thumbnail of Induction of Nitric Oxide and TNF-Α in Newcastle Disease Virus (NDV) AF2240 Infected RAW 264.7 Macrophages and their Cytotoxic Activity on MDA-MB-231 Breast Cancer Cell Line

Journal of Cancer Science and Therapy

Background: It is well known that Newcastle Disease Virus (NDV) AF2240 has antitumor activity aga... more Background: It is well known that Newcastle Disease Virus (NDV) AF2240 has antitumor activity against breast cancer cell lines. Several studies have thought this activity depend on direct viral oncolysis, which may not be the only factor that play a role in the antitumor effect of the virus. However, the mechanism underlying its antitumor effect is largely unresolved. In line with this observation, we postulated that NDV AF2240 may induce an in vitro production of Nitric Oxide (NO) and Tumor Necrosis Factor (TNF-α) in RAW 264.7 macrophages for cytotoxic activity on breast cancer cells. Methods: NDV AF2240 were subjected to different experimental conditions (Live, UV-inactivated and Heatinactivated), different Heamagglutination (HA) titer of the virus were co-cultured with RAW 264.7 cells to get the cell-free supernatant. Levels of TNF-α, and NO production were measured by ELISA and Griess assay respectively. MTT assay was later used to determine their cytotoxicity on MDA-MB-231 brea...

Research paper thumbnail of Low dose of tocotrienols protects osteoblasts against oxidative stress

La Clinica terapeutica, 2011

Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. ... more Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of a-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Toxicity tests were carried out on hydrogen peroxide (H(2)O(2)), a-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC(50)) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of a-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC50 of H(2)O(2) for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H(2)O(2). The IC(50) after 2 hours and 24 hours incubation time for H(2)O(2) were 490 µM and 280 µM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC(50) of 290 µM after 24 hours incubation time while a-tocopherol was not ...

Research paper thumbnail of Inhibitory and Apoptosis-Inducing Effects of Newcastle Disease Virus Strain AF2240 on Mammary Carcinoma Cell Line

BioMed Research International, 2015

Breast cancer is the malignant tumour that developed from cells of the breast and is the first le... more Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women worldwide. Surgery, radiotherapy, and chemotherapy are the available treatments for breast cancer, but these were reported to have side effects. Newcastle disease virus (NDV) known as Avian paramyxovirus type-1 (APMV1) belongs to the genus Avulavirus in a family Paramyxoviridae. NDV is shown to be a promising anticancer agent, killing tumour cells while sparing normal cells unharmed. In this study, the oncolytic and cytotoxic activities of NDV AF2240 strain were evaluated on MDA-MB-231, human mammary carcinoma cell line, using MTT assay, and its inhibitory effects were further studied using proliferation and migration assays. Morphological and apoptotic-inducing effects of NDV on MD-MB-231 cells were observed using phase contrast and fluorescence microscopes. Detection of DNA fragmentation was done following terminal deoxyribonucleotide transferasemediated Br-dUTP nick end labeling staining (TUNEL) assay, which confirmed that the mode of death was through apoptosis and was quantified by flow cytometry. Furthermore, analysis of cellular DNA content demonstrated that the virus caused an increase in the sub-G1 phase (apoptotic peak) of the cell cycle. It appears that NDV AF2240 strain is a potent anticancer agent that induced apoptosis in time-dependent manner.

Research paper thumbnail of Detection and Quantification of Fragmented Dna in Breast Cancer Cell Line Treated with Newcastle Disease Virus (NDV)

Introduction: Breast cancer is the malignant tumour that developed from cells of the breast. This... more Introduction: Breast cancer is the malignant tumour that developed from cells of the breast. This disease, is the first leading cause of cancer death among women globally and the most prevalent cancer in the world. Surgery, radiotherapy, and chemotherapy are the available treatment for breast cancer, but these were reported to have some side effects. Newcastle disease virus (NDV), also known as avian paramyxovirus type-1, APMV-1 is belonging to the genus Avulavirus in a family Paramyxoviridae. The virus is of economic important in that, it result in significant loss to the poultry industry, due to its effect on their brain, gastrointestinal and respiratory tracts. However, exposure to human results in a mild conjunctivitis, laryngitis and influenza-like symptoms. NDV showed promising as an anti-cancer agent as it kill tumour cells while leaving the normal cells unharm. Several strains of NDV such as 73-T, MTH68 and HUJ have been shown to posses the ability to kill cancer cells. Addi...

Research paper thumbnail of Umar et al Poster NPS-UPM

Breast cancer is the malignant tumour that developed from cells of the breast and is the first le... more Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women globally. Surgery, radiotherapy, and chemotherapy are the available treatment for breast cancer, but these were reported to have some side effects. Newcastle disease virus (NDV), also known as avian paramyxovirus type-1,is belong to the genus Avulavirus in a family Paramyxoviridae. The virus is of economic important to the poultry industry as it results on its significant damages. However, exposure to human results in a mild conjunctivitis, laryngitis and influenza-like symptoms. NDV showed promising as anti-cancer agent, killing tumour cells while sparing normal cells unharm. Several strains of NDV such as 73-T, MTH68 and HUJ have been shown to posses the ability to kill cancer cells. Additionally, three (3) Malaysian strains, AF2240, S and V4-UPM, have also been studied on different cancer cell lines. Therefore, our objective was to investigate w...

Research paper thumbnail of Effects of Low-Dose versus High-Dose γ-Tocotrienol on the Bone Cells Exposed to the Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis

Evidence-Based Complementary and Alternative Medicine, 2012

Oxidative stress and apoptosis can disrupt the bone formation activity of osteoblasts which can l... more Oxidative stress and apoptosis can disrupt the bone formation activity of osteoblasts which can lead to osteoporosis. This study was conducted to investigate the effects of γ-tocotrienol on lipid peroxidation, antioxidant enzymes activities, and apoptosis of osteoblast exposed to hydrogen peroxide (H 2 O 2 ). Osteoblasts were treated with 1, 10, and 100 μM of γ-tocotrienol for 24 hours before being exposed to 490 μM (IC 50 ) H 2 O 2 for 2 hours. Results showed that γ-tocotrienol prevented the malondialdehyde (MDA) elevation induced by H 2 O 2 in a dose-dependent manner. As for the antioxidant enzymes assays, all doses of γ-tocotrienol were able to prevent the reduction in SOD and CAT activities, but only the dose of 1 μM of GTT was able to prevent the reduction in GPx. As for the apoptosis assays, γ-tocotrienol was able to reduce apoptosis at the dose of 1 and 10 μM. However, the dose of 100 μM of γ-tocotrienol induced an even higher apoptosis than H 2 O 2 . In conclusion, low doses of γ-tocotrienol offered protection for osteoblasts against H 2 O 2 toxicity, but itself caused toxicity at the high doses.

Research paper thumbnail of Induction of Nitric Oxide and TNF-Α in Newcastle Disease Virus (NDV) AF2240 Infected RAW 264.7 Macrophages and their Cytotoxic Activity on MDA-MB-231 Breast Cancer Cell Line

Journal of Cancer Science and Therapy

Background: It is well known that Newcastle Disease Virus (NDV) AF2240 has antitumor activity aga... more Background: It is well known that Newcastle Disease Virus (NDV) AF2240 has antitumor activity against breast cancer cell lines. Several studies have thought this activity depend on direct viral oncolysis, which may not be the only factor that play a role in the antitumor effect of the virus. However, the mechanism underlying its antitumor effect is largely unresolved. In line with this observation, we postulated that NDV AF2240 may induce an in vitro production of Nitric Oxide (NO) and Tumor Necrosis Factor (TNF-α) in RAW 264.7 macrophages for cytotoxic activity on breast cancer cells. Methods: NDV AF2240 were subjected to different experimental conditions (Live, UV-inactivated and Heatinactivated), different Heamagglutination (HA) titer of the virus were co-cultured with RAW 264.7 cells to get the cell-free supernatant. Levels of TNF-α, and NO production were measured by ELISA and Griess assay respectively. MTT assay was later used to determine their cytotoxicity on MDA-MB-231 brea...