Dana Manda - Academia.edu (original) (raw)

Papers by Dana Manda

Research paper thumbnail of Effect of Using Calcium Fructoborate on Weaned Piglet Bone Mineralization

The purpose of the study was to evaluate the effect of two calcium sources (calcium carbonate and... more The purpose of the study was to evaluate the effect of two calcium sources (calcium carbonate and calcium fructoborate) on bone mineralization in weaned piglets, starting from the hypothesis that boron might improve calcium (Ca) absorption in the bone tissue. Two groups of weaned piglets (4 piglets per group) with an initial average weight of 15.5 ± 0.8 kg were fed for 35 days on a corn-soybean mealbased diet. The diets differed by the calcium source: calcium carbonate for the control, C group and calcium fructoborate for the experimental, E group. Serum Ca (mg/dL), determined in the end of the experiment didn't differ significantly (p≤ 0.05): 10.70 ± 0.6 (C) and 11.81 ± 0.8 (E). Femur and tibia dry matter, fat and ash didn't differ significantly (p≤ 0.05) between the two groups. The conclusion of the experiment is that calcium fructoborate is an alternative source of calcium, but it didn't improve calcium absorption in the bone. The experiment was conducted within CEEX project 110-NUTRIMINEROS.

Research paper thumbnail of Enos, P22phox and apoE gene polymorphism associations with circulating hormone levels in the elderly

10th European Congress of Endocrinology, May 1, 2008

... Olga Ianas 1 , Dana Manda 1 , Constantina Heltianu 2 , Anca Sima 2 , Monica Raicu 2 , Marcela... more ... Olga Ianas 1 , Dana Manda 1 , Constantina Heltianu 2 , Anca Sima 2 , Monica Raicu 2 , Marcela Covic 3 & Constantin Dumitrache 1. 1 CI Parhon Institute of Endocrinology, Bucharest, Romania; 2 N. Simionescu Institute of Cellular Biology and Pathology, Bucharest, Romania; 3 ...

Research paper thumbnail of Does serum Galectin-3 add value in thyroid cancer diagnosis?

Endocrine Abstracts, May 13, 2016

Research paper thumbnail of Sex specific association between estrogen receptor alpha PvuII and XbaI gene polymorphisms and hormones

12th European Congress of Endocrinology, Apr 1, 2010

Objective: To explore if the polymorphisms of the estrogen receptor alpha gene, XbaI (IVS1-351 A/... more Objective: To explore if the polymorphisms of the estrogen receptor alpha gene, XbaI (IVS1-351 A/G) and PvuII (IVS1-397 T/C) are associated with age-dependent changes in hormone levels for a better understanding of the biological actions of estrogens.

Research paper thumbnail of The effect of polyunsaturated fatty acids enriched eggs on lipid profile and inflammatory markers

10th European Congress of Endocrinology, May 1, 2008

Research paper thumbnail of Matrix metalloproteinase-9 (MMP-9) promoter -1562C/T functional polymorphism is associated with an increased risk to develop micropapillary thyroid carcinoma

Cancer Biomarkers, Jul 18, 2022

BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of tumor initiation and p... more BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of tumor initiation and progression. The MMP-9 promoter -1562C/T functional polymorphism increases gene expression and was identified as a susceptibility factor for various cancers. OBJECTIVE: To evaluate the influence of the MMP-9 promoter genotype on the risk of developing papillary thyroid cancer (PTC) and to correlate cancer patient genotype with the clinical and pathological phenotype. METHODS: We evaluated 236 patients with nodular thyroid disease pre-thyroidectomy (119 benign disease, 117 PTC). Genomic DNA was isolated from whole blood and the MMP-9 -1562C/T genotype was evaluated by PCR-RFLP analysis. RESULTS: Genotype frequencies were in Hardy-Weinberg equilibrium for all groups. The T allele was significantly more frequent in cancer compared to benign disease (17.5% vs 10.1%), p= 0.019. Patients with the CT or CT+TT genotype had an increased risk of developing PTC, specifically micropapillary thyroid carcinoma (MPTC) (CT genotype: OR = 6.467, p= 0.00006; CT+TT: OR = 6.859, p= 0.00002), but not more advanced stages (CT: p= 0.094; CT+TT: p= 0.157). The -1562C/T genotype did not significantly correlate with tumor histological subtype, invasion or TNM stage. CONCLUSION: The MMP-9 -1562C/T functional polymorphism may indicate susceptibility to develop thyroid cancer, specifically intrathyroidal clinically non-relevant MPTC. This suggests that although this genotype might be a predisposing factor, other genetic/epigenetic events are needed for cancer progression.

Research paper thumbnail of MMP-9 1562 C/T polymorphism may be associated with an increased susceptibility to develop micropapillary thyroid cancer but not more advanced tumours

Endocrine Abstracts, Aug 21, 2020

Research paper thumbnail of Prevalence of BRAF V600E mutation in Romanian thyroid tumors patients

Endocrine Abstracts, Feb 11, 2016

Research paper thumbnail of Analytical validation of a thyroglobulin imunoassay in fine-needle aspirate washout of suspicious lymph nodes in patients with differentiated thyroid cancer

Clinica Chimica Acta, Jun 1, 2019

Background-aim Free testosterone accounts for approximately 2% of the total testosterone count an... more Background-aim Free testosterone accounts for approximately 2% of the total testosterone count and is important in the diagnosis of many diseases including hypogonadism in men and polycystic ovary syndrome or hirsutism in women. The detection of the free forms of testosterone in serum is rather challenging and several methods are available. The purpose of this study was to evaluate an automated competitive chemiluminescent immunoassay (IDS-iSYS) as compared to a radioimmunoassay (RIA) method.

Research paper thumbnail of Association between preoperative serum MMP-9 and histopathological features of thyroid tumors

Endocrine Abstracts, May 3, 2017

Research paper thumbnail of The Relationship between Advanced Oxidation Protein Products, Vascular Calcifications and Arterial Stiffness in Predialysis Chronic Kidney Disease Patients

Medicina-lithuania, May 6, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Carriers of a pathological variant in CYP21A2 gene- clinical and hormonal status

Research paper thumbnail of Prevalence and clinico-pathological correlations of BRAF V600E and TERT promoter mutations in differentiated thyroid cancer in Romania

Research paper thumbnail of Serum Matrix metalloproteinase-9 (MMP-9) can help identify patients with papillary thyroid cancer at high risk of persistent disease: Value and limitations of a potential marker of neoplasia

Cancer Biomarkers

BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of invasion and metastasi... more BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of invasion and metastasis in neoplasia. In thyroid cancer expression levels correlate with aggressiveness but data on peripheral MMP-9 levels are less definitive. OBJECTIVE: Prospective study evaluating serum MMP-9 in the diagnosis and prognosis of papillary thyroid cancer. METHODS: Serum samples of MMP-9 were drawn before surgery in 185 consecutively enrolled patients with nodular thyroid disease, stratified on pathology as benign disease (N= 88) and papillary thyroid cancer (N= 97). Serum MMP-9 was measured by an immunometric assay. RESULTS: MMP-9 levels were not different between benign vs malignant pathology (p= 0.3). In papillary thyroid cancer there was no significant difference in MMP-9 levels between histologies, TNM stage and invasive/non-invasive cancers. High-risk patients with multiple features of aggressiveness had significantly higher MMP-9 levels compared to low-intermediate risk patients (767.5 ...

Research paper thumbnail of The spectrum of CYP21A2 copy number variations and gene mutations by MLPA in a pediatric Romanian population with 21-hydroxylase deficiency

Research paper thumbnail of Study of CYP17 and PSA G158A polymorphisms in prostate cancer

Endocrine Abstracts, 2017

Research paper thumbnail of Alteration of melatonin and cortisol rhythms in primary epilepsy

The present study has been performed in response to the increasing interest shown in involvement ... more The present study has been performed in response to the increasing interest shown in involvement of melatonin in neuropsychiatric disorders as a circadian effector. The study evaluated the day-night variations in urine both 6-sulfatoximelatonin (aMLT6S) and cortisol in healthy children and children with primary epilepsy in order to clarify the implications of circadian neuroendocrine axis. Study groups included 19 children with primary epilepsy and 17 healthy children. As expected a marked 24 h variation in hormone levels was found in the healthy group. In patients with epilepsy diurnal variation of hormone secretions were altered from the loss of circadian rhythm to an inversed one. In healthy children, 6-sulfatoximelatonin exhibited distinct diurnal variation with the nocturnal aMLT6s mean concentration of eight-time higher than the daytime mean. In children with primary epilepsy, there was a great individual variation in nocturnal aMLT6s excretion, from 9 to 218 ng/ml: six childr...

Research paper thumbnail of Effect of melatonin on gene expression and mineralization in mouse osteoblast culture

Research paper thumbnail of Interrelation between Histamine and Serotonin, Dopamine, GABA, IGF-1 in a Growth Hormone (GH) Deficient Group under rh-GH Replacement Therapy

Aim: To evaluate relationship between histamine (HIST), serotonin (5-HT), dopamine (DA), gamma-am... more Aim: To evaluate relationship between histamine (HIST), serotonin (5-HT), dopamine (DA), gamma-amino-butyric acid (GABA) and IGF-1 in 20 GH-deficient boys. Research design and methods: This study included 20 boys (5-14 years) with GH deficit clinically established and a 10 matched normal group with no endocrine dysfunction. All of GH deficient patients underwent GH replacement therapy. In 2017, all subjects were tested by analytical methods for blood: HIST,GABA,DA,5-HT,IGF-1. Results: We divided this study group into a low HIST lot 1 (10 subjects): HIST median: 3.48 nM/L and a high HIST lot 2 (10 subjects): HIST median: 11nM/L. Median parameters in lot 1 vs. lot 2 were: 5-HT: 212.5 vs. 370ng/mL, DA: 30 vs. 45pg/ml, GABA: 30 vs. 56.5ng/mL, IGF-1: 373.5 vs. 200ng/mL. Median values in normal subjects were as it follows: HIST: 5.55nM/L; 5-HT: 235.5ng/mL; DA: 31.5pg/mL; GABA: 81ng /mL. T-Test rev...

Research paper thumbnail of Prolactinoma cell culture: experimental model for tumor growth

Research paper thumbnail of Effect of Using Calcium Fructoborate on Weaned Piglet Bone Mineralization

The purpose of the study was to evaluate the effect of two calcium sources (calcium carbonate and... more The purpose of the study was to evaluate the effect of two calcium sources (calcium carbonate and calcium fructoborate) on bone mineralization in weaned piglets, starting from the hypothesis that boron might improve calcium (Ca) absorption in the bone tissue. Two groups of weaned piglets (4 piglets per group) with an initial average weight of 15.5 ± 0.8 kg were fed for 35 days on a corn-soybean mealbased diet. The diets differed by the calcium source: calcium carbonate for the control, C group and calcium fructoborate for the experimental, E group. Serum Ca (mg/dL), determined in the end of the experiment didn't differ significantly (p≤ 0.05): 10.70 ± 0.6 (C) and 11.81 ± 0.8 (E). Femur and tibia dry matter, fat and ash didn't differ significantly (p≤ 0.05) between the two groups. The conclusion of the experiment is that calcium fructoborate is an alternative source of calcium, but it didn't improve calcium absorption in the bone. The experiment was conducted within CEEX project 110-NUTRIMINEROS.

Research paper thumbnail of Enos, P22phox and apoE gene polymorphism associations with circulating hormone levels in the elderly

10th European Congress of Endocrinology, May 1, 2008

... Olga Ianas 1 , Dana Manda 1 , Constantina Heltianu 2 , Anca Sima 2 , Monica Raicu 2 , Marcela... more ... Olga Ianas 1 , Dana Manda 1 , Constantina Heltianu 2 , Anca Sima 2 , Monica Raicu 2 , Marcela Covic 3 & Constantin Dumitrache 1. 1 CI Parhon Institute of Endocrinology, Bucharest, Romania; 2 N. Simionescu Institute of Cellular Biology and Pathology, Bucharest, Romania; 3 ...

Research paper thumbnail of Does serum Galectin-3 add value in thyroid cancer diagnosis?

Endocrine Abstracts, May 13, 2016

Research paper thumbnail of Sex specific association between estrogen receptor alpha PvuII and XbaI gene polymorphisms and hormones

12th European Congress of Endocrinology, Apr 1, 2010

Objective: To explore if the polymorphisms of the estrogen receptor alpha gene, XbaI (IVS1-351 A/... more Objective: To explore if the polymorphisms of the estrogen receptor alpha gene, XbaI (IVS1-351 A/G) and PvuII (IVS1-397 T/C) are associated with age-dependent changes in hormone levels for a better understanding of the biological actions of estrogens.

Research paper thumbnail of The effect of polyunsaturated fatty acids enriched eggs on lipid profile and inflammatory markers

10th European Congress of Endocrinology, May 1, 2008

Research paper thumbnail of Matrix metalloproteinase-9 (MMP-9) promoter -1562C/T functional polymorphism is associated with an increased risk to develop micropapillary thyroid carcinoma

Cancer Biomarkers, Jul 18, 2022

BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of tumor initiation and p... more BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of tumor initiation and progression. The MMP-9 promoter -1562C/T functional polymorphism increases gene expression and was identified as a susceptibility factor for various cancers. OBJECTIVE: To evaluate the influence of the MMP-9 promoter genotype on the risk of developing papillary thyroid cancer (PTC) and to correlate cancer patient genotype with the clinical and pathological phenotype. METHODS: We evaluated 236 patients with nodular thyroid disease pre-thyroidectomy (119 benign disease, 117 PTC). Genomic DNA was isolated from whole blood and the MMP-9 -1562C/T genotype was evaluated by PCR-RFLP analysis. RESULTS: Genotype frequencies were in Hardy-Weinberg equilibrium for all groups. The T allele was significantly more frequent in cancer compared to benign disease (17.5% vs 10.1%), p= 0.019. Patients with the CT or CT+TT genotype had an increased risk of developing PTC, specifically micropapillary thyroid carcinoma (MPTC) (CT genotype: OR = 6.467, p= 0.00006; CT+TT: OR = 6.859, p= 0.00002), but not more advanced stages (CT: p= 0.094; CT+TT: p= 0.157). The -1562C/T genotype did not significantly correlate with tumor histological subtype, invasion or TNM stage. CONCLUSION: The MMP-9 -1562C/T functional polymorphism may indicate susceptibility to develop thyroid cancer, specifically intrathyroidal clinically non-relevant MPTC. This suggests that although this genotype might be a predisposing factor, other genetic/epigenetic events are needed for cancer progression.

Research paper thumbnail of MMP-9 1562 C/T polymorphism may be associated with an increased susceptibility to develop micropapillary thyroid cancer but not more advanced tumours

Endocrine Abstracts, Aug 21, 2020

Research paper thumbnail of Prevalence of BRAF V600E mutation in Romanian thyroid tumors patients

Endocrine Abstracts, Feb 11, 2016

Research paper thumbnail of Analytical validation of a thyroglobulin imunoassay in fine-needle aspirate washout of suspicious lymph nodes in patients with differentiated thyroid cancer

Clinica Chimica Acta, Jun 1, 2019

Background-aim Free testosterone accounts for approximately 2% of the total testosterone count an... more Background-aim Free testosterone accounts for approximately 2% of the total testosterone count and is important in the diagnosis of many diseases including hypogonadism in men and polycystic ovary syndrome or hirsutism in women. The detection of the free forms of testosterone in serum is rather challenging and several methods are available. The purpose of this study was to evaluate an automated competitive chemiluminescent immunoassay (IDS-iSYS) as compared to a radioimmunoassay (RIA) method.

Research paper thumbnail of Association between preoperative serum MMP-9 and histopathological features of thyroid tumors

Endocrine Abstracts, May 3, 2017

Research paper thumbnail of The Relationship between Advanced Oxidation Protein Products, Vascular Calcifications and Arterial Stiffness in Predialysis Chronic Kidney Disease Patients

Medicina-lithuania, May 6, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Carriers of a pathological variant in CYP21A2 gene- clinical and hormonal status

Research paper thumbnail of Prevalence and clinico-pathological correlations of BRAF V600E and TERT promoter mutations in differentiated thyroid cancer in Romania

Research paper thumbnail of Serum Matrix metalloproteinase-9 (MMP-9) can help identify patients with papillary thyroid cancer at high risk of persistent disease: Value and limitations of a potential marker of neoplasia

Cancer Biomarkers

BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of invasion and metastasi... more BACKGROUND: Matrix metalloproteinase-9 (MMP-9) is an important mediator of invasion and metastasis in neoplasia. In thyroid cancer expression levels correlate with aggressiveness but data on peripheral MMP-9 levels are less definitive. OBJECTIVE: Prospective study evaluating serum MMP-9 in the diagnosis and prognosis of papillary thyroid cancer. METHODS: Serum samples of MMP-9 were drawn before surgery in 185 consecutively enrolled patients with nodular thyroid disease, stratified on pathology as benign disease (N= 88) and papillary thyroid cancer (N= 97). Serum MMP-9 was measured by an immunometric assay. RESULTS: MMP-9 levels were not different between benign vs malignant pathology (p= 0.3). In papillary thyroid cancer there was no significant difference in MMP-9 levels between histologies, TNM stage and invasive/non-invasive cancers. High-risk patients with multiple features of aggressiveness had significantly higher MMP-9 levels compared to low-intermediate risk patients (767.5 ...

Research paper thumbnail of The spectrum of CYP21A2 copy number variations and gene mutations by MLPA in a pediatric Romanian population with 21-hydroxylase deficiency

Research paper thumbnail of Study of CYP17 and PSA G158A polymorphisms in prostate cancer

Endocrine Abstracts, 2017

Research paper thumbnail of Alteration of melatonin and cortisol rhythms in primary epilepsy

The present study has been performed in response to the increasing interest shown in involvement ... more The present study has been performed in response to the increasing interest shown in involvement of melatonin in neuropsychiatric disorders as a circadian effector. The study evaluated the day-night variations in urine both 6-sulfatoximelatonin (aMLT6S) and cortisol in healthy children and children with primary epilepsy in order to clarify the implications of circadian neuroendocrine axis. Study groups included 19 children with primary epilepsy and 17 healthy children. As expected a marked 24 h variation in hormone levels was found in the healthy group. In patients with epilepsy diurnal variation of hormone secretions were altered from the loss of circadian rhythm to an inversed one. In healthy children, 6-sulfatoximelatonin exhibited distinct diurnal variation with the nocturnal aMLT6s mean concentration of eight-time higher than the daytime mean. In children with primary epilepsy, there was a great individual variation in nocturnal aMLT6s excretion, from 9 to 218 ng/ml: six childr...

Research paper thumbnail of Effect of melatonin on gene expression and mineralization in mouse osteoblast culture

Research paper thumbnail of Interrelation between Histamine and Serotonin, Dopamine, GABA, IGF-1 in a Growth Hormone (GH) Deficient Group under rh-GH Replacement Therapy

Aim: To evaluate relationship between histamine (HIST), serotonin (5-HT), dopamine (DA), gamma-am... more Aim: To evaluate relationship between histamine (HIST), serotonin (5-HT), dopamine (DA), gamma-amino-butyric acid (GABA) and IGF-1 in 20 GH-deficient boys. Research design and methods: This study included 20 boys (5-14 years) with GH deficit clinically established and a 10 matched normal group with no endocrine dysfunction. All of GH deficient patients underwent GH replacement therapy. In 2017, all subjects were tested by analytical methods for blood: HIST,GABA,DA,5-HT,IGF-1. Results: We divided this study group into a low HIST lot 1 (10 subjects): HIST median: 3.48 nM/L and a high HIST lot 2 (10 subjects): HIST median: 11nM/L. Median parameters in lot 1 vs. lot 2 were: 5-HT: 212.5 vs. 370ng/mL, DA: 30 vs. 45pg/ml, GABA: 30 vs. 56.5ng/mL, IGF-1: 373.5 vs. 200ng/mL. Median values in normal subjects were as it follows: HIST: 5.55nM/L; 5-HT: 235.5ng/mL; DA: 31.5pg/mL; GABA: 81ng /mL. T-Test rev...

Research paper thumbnail of Prolactinoma cell culture: experimental model for tumor growth