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Papers by Maneekarn Namsa-aid
World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences, 2019
Trends in Sciences
Among soil isolated species of actinomycetes bacteria that affected the growth of aflatoxin produ... more Among soil isolated species of actinomycetes bacteria that affected the growth of aflatoxin producing mold; Aspergillus flavus TISTR 3041, the isolate SP-O2 showed a promising inhibitory activity on the dual culture assay. This isolated bacterium was characterized on various International Streptomyces Project media. The macroscopic morphological analysis showed mycelium, sporogenesis, and orangish pink of colonial pigment. The scanning electron microscope (SEM) photograph showed that the sporogenesis has occurred by shrinking of both sides of aerial mycelium. After the sporogenesis has been completed, the fragmentation of altered mycelium started from the tips of aerial mycelium to produce 0.8-1 micrometer fragmented spores. The 16S rRNA sequences analysis revealed that isolate SP-O2 shared a 99.93 % (1 mismatch/1436 nt) similarity with Streptomyces spectabilis NBRC 13424 AB184393. Co-culture liquid assay was analyzed for the aflatoxin produced by A. flavus in the presence of Strept...
Terrein is a fungal metabolite that has already reported with anticancer properties. However, the... more Terrein is a fungal metabolite that has already reported with anticancer properties. However, the effect on the aggressive phenotype of cancer cells has not elucidated yet. In the present study, the cytotoxicity of terrein was firstly determined against lung cancer cells (A549) model and comparing with several normal cell lines (Vero, L6, and H9C2 cells). The data demonstrated that terrein had a specific effect on A549 cells relative to normal cells with high selectivity index values. Then, the hypoxic model that recognized to induce aggressive abilities was established in A549 cells by cobalt chloride (CoCl2) stimulation. With this model, terrein could reduce HIF-1α, a marker of hypoxia, and inhibit both migration and invasion of which the effect on invasion are more explicit. Then, our results demonstrated that terrein has a potential new role as the anti-aggressive phenotype by inhibiting cancer cell migration and invasion through HIF-1α reduction.
Trends in Sciences
The macrolide brefeldin A (BFA, 1) exhibited high cytotoxicity against KB cells. However, it was ... more The macrolide brefeldin A (BFA, 1) exhibited high cytotoxicity against KB cells. However, it was also toxic against non-cancerous cells. In order to lower toxicity against normal cells while maintaining the cytotoxic potency to the cancer cells, structural modification of this compound was undertaken. Starting from compound 1, the analogues 2-13 were synthesized and evaluated for cytotoxicity against KB cells. The analogue 2 exhibited the most potent cytotoxicity against KB cells, with an IC50 value of 0.034 nM, 67-fold more active than its parent compound 1. It was 41764 and 8235 fold more active than the standard drugs ellipticine and doxorubicin, respectively. The higher cytotoxicity against KB cells and lower toxicity against Vero cells of analogue 2 than those of the parent compound 1 contributed to its exceptionally high selectivity index of 9117. The results suggested that this analogue might be utilized to develop a new candidate for an anticancer drug. HIGHLIGHTS Oral cance...
World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences, 2019
Trends in Sciences
Among soil isolated species of actinomycetes bacteria that affected the growth of aflatoxin produ... more Among soil isolated species of actinomycetes bacteria that affected the growth of aflatoxin producing mold; Aspergillus flavus TISTR 3041, the isolate SP-O2 showed a promising inhibitory activity on the dual culture assay. This isolated bacterium was characterized on various International Streptomyces Project media. The macroscopic morphological analysis showed mycelium, sporogenesis, and orangish pink of colonial pigment. The scanning electron microscope (SEM) photograph showed that the sporogenesis has occurred by shrinking of both sides of aerial mycelium. After the sporogenesis has been completed, the fragmentation of altered mycelium started from the tips of aerial mycelium to produce 0.8-1 micrometer fragmented spores. The 16S rRNA sequences analysis revealed that isolate SP-O2 shared a 99.93 % (1 mismatch/1436 nt) similarity with Streptomyces spectabilis NBRC 13424 AB184393. Co-culture liquid assay was analyzed for the aflatoxin produced by A. flavus in the presence of Strept...
Terrein is a fungal metabolite that has already reported with anticancer properties. However, the... more Terrein is a fungal metabolite that has already reported with anticancer properties. However, the effect on the aggressive phenotype of cancer cells has not elucidated yet. In the present study, the cytotoxicity of terrein was firstly determined against lung cancer cells (A549) model and comparing with several normal cell lines (Vero, L6, and H9C2 cells). The data demonstrated that terrein had a specific effect on A549 cells relative to normal cells with high selectivity index values. Then, the hypoxic model that recognized to induce aggressive abilities was established in A549 cells by cobalt chloride (CoCl2) stimulation. With this model, terrein could reduce HIF-1α, a marker of hypoxia, and inhibit both migration and invasion of which the effect on invasion are more explicit. Then, our results demonstrated that terrein has a potential new role as the anti-aggressive phenotype by inhibiting cancer cell migration and invasion through HIF-1α reduction.
Trends in Sciences
The macrolide brefeldin A (BFA, 1) exhibited high cytotoxicity against KB cells. However, it was ... more The macrolide brefeldin A (BFA, 1) exhibited high cytotoxicity against KB cells. However, it was also toxic against non-cancerous cells. In order to lower toxicity against normal cells while maintaining the cytotoxic potency to the cancer cells, structural modification of this compound was undertaken. Starting from compound 1, the analogues 2-13 were synthesized and evaluated for cytotoxicity against KB cells. The analogue 2 exhibited the most potent cytotoxicity against KB cells, with an IC50 value of 0.034 nM, 67-fold more active than its parent compound 1. It was 41764 and 8235 fold more active than the standard drugs ellipticine and doxorubicin, respectively. The higher cytotoxicity against KB cells and lower toxicity against Vero cells of analogue 2 than those of the parent compound 1 contributed to its exceptionally high selectivity index of 9117. The results suggested that this analogue might be utilized to develop a new candidate for an anticancer drug. HIGHLIGHTS Oral cance...