Manjurul Haque - Academia.edu (original) (raw)

Papers by Manjurul Haque

Research paper thumbnail of A gastrointestinal nematode in pregnant and lactating mice alters maternal and neonatal microbiomes

International Journal for Parasitology

Research paper thumbnail of Maternal Gastrointestinal Nematode Infection Up-regulates Expression of Genes Associated with Long-Term Potentiation in Perinatal Brains of Uninfected Developing Pups

Research paper thumbnail of Bovine Neonatal Monocytes Display Phenotypic Differences Compared With Adults After Challenge With the Infectious Abortifacient Agent Neospora caninum

Frontiers in Immunology

The neonatal period represents a window of susceptibility for ruminants given the abundance of in... more The neonatal period represents a window of susceptibility for ruminants given the abundance of infectious challenges in their environment. Maternal transfer of immunity does not occur in utero but post-parturition, however this does not compensate for potential deficits in the cellular compartment. Here we present a cellular and transcriptomic study to investigate if there is an age-related difference in the monocyte response in cattle during intra-cellular protozoan infection. We utilized Neospora caninum, an obligate intracellular protozoan parasite that causes abortion and negative economic impacts in cattle worldwide, to study these responses. We found neonatal animals had a significant greater percentage of CD14 + monocytes with higher CD80 cell surface expression. Adult monocytes harbored more parasites compared to neonatal monocytes; additionally greater secretion of IL-1β was observed in neonates. Microarray analysis revealed neonates have 535 genes significantly upregulated compared to adult with 23 upregulated genes. Biological pathways involved in immune response were evaluated and both age groups showed changes in the upregulation of tyrosine phosphorylation of STAT protein and JAK-STAT cascade pathways. However, the extent to which these pathways were upregulated in neonates was much greater. Our findings suggest that neonates are more resistant to cellular invasion with protozoan parasites and that the magnitude of the responses is related to significant changes in the JAK-STAT network.

Research paper thumbnail of Differential expression of genes in fetal brain as a consequence of maternal protein deficiency and nematode infection

International Journal for Parasitology

Research paper thumbnail of Concurrent detection of Theileria organism from definitive host and vector tick

The Indian veterinary journal

Research paper thumbnail of Incidence of gastrointestinal parasites in dairy animals of western plains of Punjab. J Vet Parasitol

Journal of Veterinary Parasitology

A total of 628 faecal samples were collected from cattle and buffaloes reared in western plains o... more A total of 628 faecal samples were collected from cattle and buffaloes reared in western plains of Punjab. The prevalence of gastrointestinal (GI) parasitic infections in adult animals was 37.97%. Strongyle (18.99%) the most prevalent GI parasite followed by Eimeria sp. (13.42%), Moniezia expansa (5.57%), amphistomes (3.80%), Strongyloides papillosus (1.01%), Trichuris sp. (0.76%) and Fasciola sp. (0.51%). In calves the prevalence of Toxocara vitulorum, strongyle, S. papillosus, Eimeria sp. and M. expansa was 6.01, 21.89, 20.17, 53.22 and 3.43%, respectively. In calves, the overall prevalence of GI parasitic infections was found to be more as compared to adults and T. vitulorum was recorded only from calves of less than six months of age. The baseline data on the prevalence of various GI parasites of bovids generated in the current study would be of immense help in formulating control strategies against these parasites in the western plains of the state. Keywords: Buffalo, Calves, C...

Research paper thumbnail of Prevalance of gastrointestinal Parasites in Organized Dairy Farms

The Indian veterinary journal

Research paper thumbnail of Population dynamics of ticks (acari: ixodidae) infesting dairy animals of Ludhiana, Punjab

The Indian veterinary journal

Research paper thumbnail of Epidemiology and transmission factors of gastrointestinal parasites in cow calves in different agro-climatic zones of Punjab. J Vet Parasitol

Journal of Veterinary Parasitology

Epidemiology of gastrointestinal (GI) parasitic infections in cow calves, aged upto 6 months, fro... more Epidemiology of gastrointestinal (GI) parasitic infections in cow calves, aged upto 6 months, from 13 districts falling in 4 major agro-climatic zones of Punjab state of India was studied during the period from May, 2008 to April, 2009. A total of 627 faecal samples were examined and the overall prevalence of GI parasitic infection was 61.40%. The prevalence of Toxocara vitulorum, strongyle, Strongyloides spp., Eimeria spp. and Moniezia spp. Was 2.71, 23.29, 14.51, 44.18 and 3.67 per cent, respectively. The overall prevalence of parasitic infections was almost comparable and no significant difference (P≥0.05) was recorded among the two sexes of cow calves. However, strongyle and Eimeria spp. infection showed a positive correlation with age. The prevalence of various parasitic infections was found to be highest in rainy season with significant differences (P<0.01) as compared to summer and winter seasons. Among the various agro-climatic zones, highest prevalence of T. vitulorum, S...

Research paper thumbnail of Molluscicidal Effects of Methanolic Extract of Azadirachta Indica (Neem) on Snails Lymnaea Auricularia and Indoplanorbis Exustus*

The molluscicidal effect of methanolic extract of different components of neem plant (leaf, seed,... more The molluscicidal effect of methanolic extract of different components of neem plant (leaf, seed, bark and whole plant) was evaluated against snails Lymnaea auricularia and Indoplanorbis exustus. The extracts were prepared by cold extraction method by standard protocols. The concentrations of extracts used were 1:10, 1:15, 1:20, 1:25, 1:30 and 1:35 and the results were recorded at different period of exposure time (6 h, 12 h, 24 h, 48 h, 72 h and 96 h). The different concentrations of methanolic extract of neem showed variable effect on adult stages of L. auricularia and I. exustus. A total of 15 snails were used in triplicate in each treated and control group, respectively. It showed positive correlation between exposure time and mortality and negative correlation between dilution and mortality. Among the different extracts, the whole plant extract was most effective followed by seed, leaf and bark against snails L. auricularia and I. exustus. I. exustus was found to be more suscep...

Research paper thumbnail of Molluscicidal effect of ether extract of Azadirachta indica (neem) on experimentally reared snails Lymnaea auricularia and Indoplanorbis exustus

Research paper thumbnail of Canine Ehrlichiosis- an overview

Research paper thumbnail of Occurrence of parasitic infections in dogs in and around Ludhiana, Punjab (India)

A total of 634 canines were sampled for both haematological (460) and coprological (174) investig... more A total of 634 canines were sampled for both haematological (460) and coprological (174) investigations at Small Animal Clinics, GADVASU, Ludhiana, India during the year 2010. Examination of Giemsa stained peripheral blood smears exhibited 10.21% (47/460) haemoprotozoan comprizing of Babesia gibsoni (8.26%), Hepatozoon canis (1.08%), B. canis (0.65%) and Trypanosoma evansi (0.21%). B. gibsoni infection was significantly higher (p<0.05) in male dogs. Coprological examination revealed that the prevalence of gastro-intestinal parasitic infection was 24.71% (43/174). Among these Isospora spp. and Ancylostoma spp. were 2.29 and 22.41%, respectively. The present work emphasized that B. gibsoni and hookworms were most prevalent blood- and gastro-intestinal parasites in canines in and around Ludhiana, Punjab (India). Keywords: Canine, gastro-intestinal parasites, haemoprotozoa, Punjab

Research paper thumbnail of Prevalence of canine parasitic infections in and around Ludhiana, Punjab

Journal of Veterinary Parasitology

A total of 829 samples encompassing 488 blood, 207 faecal and 134 skin scrapings were examined fr... more A total of 829 samples encompassing 488 blood, 207 faecal and 134 skin scrapings were examined from dogs presented at Small Animal Clinics, GADVASU, Ludhiana. Examination of Giemsa stained peripheral blood smears revealed an overall prevalence of haemoprotozoan infections as 8.60% that comprised Trypanosoma evansi, Babesia gibsoni, B. canis, Ehrlichia canis and Hepatozoon canis as 0.40, 4.51, 1.43, 1.43 and 0.82%, respectively. Faecal floatation test revealed that the overall prevalence of GI parasitic infections was 26.09%. The prevalence of Isospora sp., Dipylidium caninum, Ancylostoma sp., Taenia sp. and Trichuris vulpis was 3.86, 0.97, 19.32, 1.45 and 0.48%, respectively. The hookworms were the most prevalent GI parasites of dogs. Sex wise and age wise no significant differences were recorded between various groups in the prevalence of GI parasitic infections. Out of a total of 134 skin scrapings examined the prevalence of mange mites was recorded to be 28.36%. The prevalence of...

Research paper thumbnail of Studies on acaricide resistance in Rhipicephalus (Boophilus) microplus against synthetic prethroids by adult immersion test with a discriminating dose

Journal of Veterinary Parasitology

Ticks and tick-borne diseases cause major economic losses affecting many domestic animals in trop... more Ticks and tick-borne diseases cause major economic losses affecting many domestic animals in tropical and subtropical countries including India. Synthetic pyrethroids particularly deltamethrin and cypermethrin have been used extensively to control Rhipicephalus (Boophilus) microplus in most parts of Punjab, India for the past decade leading to tick populations resistant against both the acaricides. Ticks (R. microplus) were collected from dairy farms at Haibbowal Dairy Complex, Ludhiana, Punjab and were subjected to adult immersion test with a discriminating dose (AITDD) against deltamethrin and cypermethrin. Results revealed a very high degree of resistance against deltamethrin (96.67%) and cypermethrin (93.33%) indicating need for adoption of alternative tick control strategy.

Research paper thumbnail of Epidemiology and seasonal dynamics of Ixodid ticks of dairy animals of Punjab state, India

The Indian journal of animal sciences

Cattle (890) and buffaloes (636) were examined from 8 districts of Punjab state, India with an ov... more Cattle (890) and buffaloes (636) were examined from 8 districts of Punjab state, India with an overall prevalence of 85.28 and 57.10% Rhipicephalus (Boophilus) microplus, 20.45 and 52.20% Hyalomma anatolicum anatolicum and 5.73 and 9.90% mixed infestation in cattle and buffalo population, respectively. R. microplus was the predominant tick in cattle population (85.28%), whereas in buffalo population R. microplus and H. a. anatolicum were almost evenly distributed. Among the different agro-climatic zones a significantly higher prevalence of H. a. anatolicum was recorded in both cattle and buffalo population of Western Plain and Western regions with comparison to other regions i.e. Central Plain and Undulating Plains of the state. H. a. anatolicum was recorded more in summer (23.1%) and monsoon (21.0%) seasons compared to winter (6.10%). In cattle, the tick infestation was similar in both the sexes whereas in buffalo, R. microplus was predominating in males and H. a. anatolicum was pr...

Research paper thumbnail of PCR-based detection of cryptic Trypanosoma Evansi Infection in cattle

The Indian veterinary journal

A PCR based assay was standardized for the detection of Trypanosoma evansi infection in blood sam... more A PCR based assay was standardized for the detection of Trypanosoma evansi infection in blood samples of apparently healthy carrier cattle from different areas of Punjab state, India. An amplicon of 237 bp size specific for T.evansi was detected in 4.8% of blood samples against the routine blood smear examination, which did not reveal the parasite in any of the samples. These results demonstrated that parasitic infection by T. evansi was present in cryptic form and PCR could detect such cases.

Research paper thumbnail of Epidemiology of gastro-intestinal parasites in naturally infected dairy animals of central plain zone, Punjab

Research paper thumbnail of Studies on the efficacy of some anticoccidial drugs in broiler chicks against Hisar isolates of Eimeria tenella

Journal of Veterinary Parasitology

Research paper thumbnail of Detection of Latent Theileria Annulata Infection in Cattle

The Indian veterinary journal

A PCR assay was standardized for the detection of Theileria annulata from blood samples of health... more A PCR assay was standardized for the detection of Theileria annulata from blood samples of healthy carrier cattle. Blood smears examination of 104 healthy carrier cow calves and adults revealed 16 animals positive for T. annulata whereas, amplification product of 721 bp size specific for T. annulata was detected from 40 samples. The results demonstrated that the PCR test was a valuable technique fordetection of carrier or low grade infection of T. annulata under field conditions.

Research paper thumbnail of A gastrointestinal nematode in pregnant and lactating mice alters maternal and neonatal microbiomes

International Journal for Parasitology

Research paper thumbnail of Maternal Gastrointestinal Nematode Infection Up-regulates Expression of Genes Associated with Long-Term Potentiation in Perinatal Brains of Uninfected Developing Pups

Research paper thumbnail of Bovine Neonatal Monocytes Display Phenotypic Differences Compared With Adults After Challenge With the Infectious Abortifacient Agent Neospora caninum

Frontiers in Immunology

The neonatal period represents a window of susceptibility for ruminants given the abundance of in... more The neonatal period represents a window of susceptibility for ruminants given the abundance of infectious challenges in their environment. Maternal transfer of immunity does not occur in utero but post-parturition, however this does not compensate for potential deficits in the cellular compartment. Here we present a cellular and transcriptomic study to investigate if there is an age-related difference in the monocyte response in cattle during intra-cellular protozoan infection. We utilized Neospora caninum, an obligate intracellular protozoan parasite that causes abortion and negative economic impacts in cattle worldwide, to study these responses. We found neonatal animals had a significant greater percentage of CD14 + monocytes with higher CD80 cell surface expression. Adult monocytes harbored more parasites compared to neonatal monocytes; additionally greater secretion of IL-1β was observed in neonates. Microarray analysis revealed neonates have 535 genes significantly upregulated compared to adult with 23 upregulated genes. Biological pathways involved in immune response were evaluated and both age groups showed changes in the upregulation of tyrosine phosphorylation of STAT protein and JAK-STAT cascade pathways. However, the extent to which these pathways were upregulated in neonates was much greater. Our findings suggest that neonates are more resistant to cellular invasion with protozoan parasites and that the magnitude of the responses is related to significant changes in the JAK-STAT network.

Research paper thumbnail of Differential expression of genes in fetal brain as a consequence of maternal protein deficiency and nematode infection

International Journal for Parasitology

Research paper thumbnail of Concurrent detection of Theileria organism from definitive host and vector tick

The Indian veterinary journal

Research paper thumbnail of Incidence of gastrointestinal parasites in dairy animals of western plains of Punjab. J Vet Parasitol

Journal of Veterinary Parasitology

A total of 628 faecal samples were collected from cattle and buffaloes reared in western plains o... more A total of 628 faecal samples were collected from cattle and buffaloes reared in western plains of Punjab. The prevalence of gastrointestinal (GI) parasitic infections in adult animals was 37.97%. Strongyle (18.99%) the most prevalent GI parasite followed by Eimeria sp. (13.42%), Moniezia expansa (5.57%), amphistomes (3.80%), Strongyloides papillosus (1.01%), Trichuris sp. (0.76%) and Fasciola sp. (0.51%). In calves the prevalence of Toxocara vitulorum, strongyle, S. papillosus, Eimeria sp. and M. expansa was 6.01, 21.89, 20.17, 53.22 and 3.43%, respectively. In calves, the overall prevalence of GI parasitic infections was found to be more as compared to adults and T. vitulorum was recorded only from calves of less than six months of age. The baseline data on the prevalence of various GI parasites of bovids generated in the current study would be of immense help in formulating control strategies against these parasites in the western plains of the state. Keywords: Buffalo, Calves, C...

Research paper thumbnail of Prevalance of gastrointestinal Parasites in Organized Dairy Farms

The Indian veterinary journal

Research paper thumbnail of Population dynamics of ticks (acari: ixodidae) infesting dairy animals of Ludhiana, Punjab

The Indian veterinary journal

Research paper thumbnail of Epidemiology and transmission factors of gastrointestinal parasites in cow calves in different agro-climatic zones of Punjab. J Vet Parasitol

Journal of Veterinary Parasitology

Epidemiology of gastrointestinal (GI) parasitic infections in cow calves, aged upto 6 months, fro... more Epidemiology of gastrointestinal (GI) parasitic infections in cow calves, aged upto 6 months, from 13 districts falling in 4 major agro-climatic zones of Punjab state of India was studied during the period from May, 2008 to April, 2009. A total of 627 faecal samples were examined and the overall prevalence of GI parasitic infection was 61.40%. The prevalence of Toxocara vitulorum, strongyle, Strongyloides spp., Eimeria spp. and Moniezia spp. Was 2.71, 23.29, 14.51, 44.18 and 3.67 per cent, respectively. The overall prevalence of parasitic infections was almost comparable and no significant difference (P≥0.05) was recorded among the two sexes of cow calves. However, strongyle and Eimeria spp. infection showed a positive correlation with age. The prevalence of various parasitic infections was found to be highest in rainy season with significant differences (P<0.01) as compared to summer and winter seasons. Among the various agro-climatic zones, highest prevalence of T. vitulorum, S...

Research paper thumbnail of Molluscicidal Effects of Methanolic Extract of Azadirachta Indica (Neem) on Snails Lymnaea Auricularia and Indoplanorbis Exustus*

The molluscicidal effect of methanolic extract of different components of neem plant (leaf, seed,... more The molluscicidal effect of methanolic extract of different components of neem plant (leaf, seed, bark and whole plant) was evaluated against snails Lymnaea auricularia and Indoplanorbis exustus. The extracts were prepared by cold extraction method by standard protocols. The concentrations of extracts used were 1:10, 1:15, 1:20, 1:25, 1:30 and 1:35 and the results were recorded at different period of exposure time (6 h, 12 h, 24 h, 48 h, 72 h and 96 h). The different concentrations of methanolic extract of neem showed variable effect on adult stages of L. auricularia and I. exustus. A total of 15 snails were used in triplicate in each treated and control group, respectively. It showed positive correlation between exposure time and mortality and negative correlation between dilution and mortality. Among the different extracts, the whole plant extract was most effective followed by seed, leaf and bark against snails L. auricularia and I. exustus. I. exustus was found to be more suscep...

Research paper thumbnail of Molluscicidal effect of ether extract of Azadirachta indica (neem) on experimentally reared snails Lymnaea auricularia and Indoplanorbis exustus

Research paper thumbnail of Canine Ehrlichiosis- an overview

Research paper thumbnail of Occurrence of parasitic infections in dogs in and around Ludhiana, Punjab (India)

A total of 634 canines were sampled for both haematological (460) and coprological (174) investig... more A total of 634 canines were sampled for both haematological (460) and coprological (174) investigations at Small Animal Clinics, GADVASU, Ludhiana, India during the year 2010. Examination of Giemsa stained peripheral blood smears exhibited 10.21% (47/460) haemoprotozoan comprizing of Babesia gibsoni (8.26%), Hepatozoon canis (1.08%), B. canis (0.65%) and Trypanosoma evansi (0.21%). B. gibsoni infection was significantly higher (p<0.05) in male dogs. Coprological examination revealed that the prevalence of gastro-intestinal parasitic infection was 24.71% (43/174). Among these Isospora spp. and Ancylostoma spp. were 2.29 and 22.41%, respectively. The present work emphasized that B. gibsoni and hookworms were most prevalent blood- and gastro-intestinal parasites in canines in and around Ludhiana, Punjab (India). Keywords: Canine, gastro-intestinal parasites, haemoprotozoa, Punjab

Research paper thumbnail of Prevalence of canine parasitic infections in and around Ludhiana, Punjab

Journal of Veterinary Parasitology

A total of 829 samples encompassing 488 blood, 207 faecal and 134 skin scrapings were examined fr... more A total of 829 samples encompassing 488 blood, 207 faecal and 134 skin scrapings were examined from dogs presented at Small Animal Clinics, GADVASU, Ludhiana. Examination of Giemsa stained peripheral blood smears revealed an overall prevalence of haemoprotozoan infections as 8.60% that comprised Trypanosoma evansi, Babesia gibsoni, B. canis, Ehrlichia canis and Hepatozoon canis as 0.40, 4.51, 1.43, 1.43 and 0.82%, respectively. Faecal floatation test revealed that the overall prevalence of GI parasitic infections was 26.09%. The prevalence of Isospora sp., Dipylidium caninum, Ancylostoma sp., Taenia sp. and Trichuris vulpis was 3.86, 0.97, 19.32, 1.45 and 0.48%, respectively. The hookworms were the most prevalent GI parasites of dogs. Sex wise and age wise no significant differences were recorded between various groups in the prevalence of GI parasitic infections. Out of a total of 134 skin scrapings examined the prevalence of mange mites was recorded to be 28.36%. The prevalence of...

Research paper thumbnail of Studies on acaricide resistance in Rhipicephalus (Boophilus) microplus against synthetic prethroids by adult immersion test with a discriminating dose

Journal of Veterinary Parasitology

Ticks and tick-borne diseases cause major economic losses affecting many domestic animals in trop... more Ticks and tick-borne diseases cause major economic losses affecting many domestic animals in tropical and subtropical countries including India. Synthetic pyrethroids particularly deltamethrin and cypermethrin have been used extensively to control Rhipicephalus (Boophilus) microplus in most parts of Punjab, India for the past decade leading to tick populations resistant against both the acaricides. Ticks (R. microplus) were collected from dairy farms at Haibbowal Dairy Complex, Ludhiana, Punjab and were subjected to adult immersion test with a discriminating dose (AITDD) against deltamethrin and cypermethrin. Results revealed a very high degree of resistance against deltamethrin (96.67%) and cypermethrin (93.33%) indicating need for adoption of alternative tick control strategy.

Research paper thumbnail of Epidemiology and seasonal dynamics of Ixodid ticks of dairy animals of Punjab state, India

The Indian journal of animal sciences

Cattle (890) and buffaloes (636) were examined from 8 districts of Punjab state, India with an ov... more Cattle (890) and buffaloes (636) were examined from 8 districts of Punjab state, India with an overall prevalence of 85.28 and 57.10% Rhipicephalus (Boophilus) microplus, 20.45 and 52.20% Hyalomma anatolicum anatolicum and 5.73 and 9.90% mixed infestation in cattle and buffalo population, respectively. R. microplus was the predominant tick in cattle population (85.28%), whereas in buffalo population R. microplus and H. a. anatolicum were almost evenly distributed. Among the different agro-climatic zones a significantly higher prevalence of H. a. anatolicum was recorded in both cattle and buffalo population of Western Plain and Western regions with comparison to other regions i.e. Central Plain and Undulating Plains of the state. H. a. anatolicum was recorded more in summer (23.1%) and monsoon (21.0%) seasons compared to winter (6.10%). In cattle, the tick infestation was similar in both the sexes whereas in buffalo, R. microplus was predominating in males and H. a. anatolicum was pr...

Research paper thumbnail of PCR-based detection of cryptic Trypanosoma Evansi Infection in cattle

The Indian veterinary journal

A PCR based assay was standardized for the detection of Trypanosoma evansi infection in blood sam... more A PCR based assay was standardized for the detection of Trypanosoma evansi infection in blood samples of apparently healthy carrier cattle from different areas of Punjab state, India. An amplicon of 237 bp size specific for T.evansi was detected in 4.8% of blood samples against the routine blood smear examination, which did not reveal the parasite in any of the samples. These results demonstrated that parasitic infection by T. evansi was present in cryptic form and PCR could detect such cases.

Research paper thumbnail of Epidemiology of gastro-intestinal parasites in naturally infected dairy animals of central plain zone, Punjab

Research paper thumbnail of Studies on the efficacy of some anticoccidial drugs in broiler chicks against Hisar isolates of Eimeria tenella

Journal of Veterinary Parasitology

Research paper thumbnail of Detection of Latent Theileria Annulata Infection in Cattle

The Indian veterinary journal

A PCR assay was standardized for the detection of Theileria annulata from blood samples of health... more A PCR assay was standardized for the detection of Theileria annulata from blood samples of healthy carrier cattle. Blood smears examination of 104 healthy carrier cow calves and adults revealed 16 animals positive for T. annulata whereas, amplification product of 721 bp size specific for T. annulata was detected from 40 samples. The results demonstrated that the PCR test was a valuable technique fordetection of carrier or low grade infection of T. annulata under field conditions.