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Papers by Manjushree Pawar

International journal of biology, pharmacy and allied sciences, Oct 14, 2023

Background: For drug discovery and development of novel therapeutic agents against diseases, unde... more Background: For drug discovery and development of novel therapeutic agents against diseases, understanding phytochemistry in plants is very crucial. Objective: The aim of this research was to identify the phytochemical profile of ethanolic extract of Convolvulus pluricaulis (CP), Michelia champaca (MC), and Chromolaena odorata (CO) by using LC-MS technique. Materials and Methods: The extract of selected plants by soxhlet extraction technique was prepared by using ethanol as a solvent. The extract was subjected to LC-MS analysis and the identified constituents were further reviewed for their pharmacological potentials. Results: LC-MS findings has provided significant contribution in identification of phytoconstituents in herbs belonging to a) phenolic compounds-flavonoids, phenylpropanoids; b) alkaloids-amide, proline and quinoline and c) pentacyclic triterpenes.

World Journal of Pharmaceutical Science and Research, 2023

Background: Cancer immunology is a rapidly growing field of research that studies interactions be... more Background: Cancer immunology is a rapidly growing field of research that studies interactions between the immune system and cancer cells. The selected plants have proven their immunomodulatory effects via cellular and humoral mediated immunity in animal models in our previous research work. Objective: To screen the ethanolic extracts of Convolvulus pluricaulis (CP), Michelia champaca (MC), Chromolaena odorata (CO) and combination of above three plants (CP+MC+CO) for in-vitro anticancer activity using breast and colon human cancer cell lines-MCF-7 and HT-29. Methods and materials: The extracts of selected plants (whole plant extract) were prepared by using maceration technique using ethanol as a solvent. The extracts were subjected for in-vitro anticancer potential using sulforhodamine B (SRB) assay against breast and colon cancer cell lines at concentrations of 10, 20, 40 and 80 µg/ml. The standard drug Adriamycin was used. Results: All the extracts inhibited percent control growth in dose dependent manner for both breast and colon cancer cell line. GI50 (Concentration of drug causing 50% inhibition of cell growth) for breast cancer cell line in CP extract was more than 80 µg/ml, and in MC, CO, and CP+MC+CO extract it was found to be 77.6, 49.5 and 78.2 µg/ml. GI50 for colon cancer cell line in all the extracts was found to be more than 80 µg/ml. Optimization in experimental conditions or turning extracts into novel drug delivery systems can enhance the medicinal effects of the plants and can reduce the GI50 concentration. Conclusion: Optimization in experimental conditions or turning extracts into novel drug delivery systems can enhance the medicinal effects of the plants and can reduce the GI50 concentration to desired standard level considering the phenolic/flavonoid richness of these plants.

International Journal of Biology, Pharmacy and Allied Scinces, 2023

Background: For drug discovery and development of novel therapeutic agents against diseases, unde... more Background: For drug discovery and development of novel therapeutic agents against diseases, understanding phytochemistry in plants is very crucial. Objective: The aim of this research was to identify the phytochemical profile of ethanolic extract of Convolvulus pluricaulis (CP), Michelia champaca (MC), and Chromolaena odorata (CO) by using LC-MS technique. Materials and Methods: The extract of selected plants by soxhlet extraction technique was prepared by using ethanol as a solvent. The extract was subjected to LC-MS analysis and the identified constituents were further reviewed for their pharmacological potentials. Results: LC-MS findings has provided significant contribution in identification of phytoconstituents in herbs belonging to a) phenolic compounds-flavonoids, phenylpropanoids; b) alkaloids-amide, proline and quinoline and c) pentacyclic triterpenes.

INTERNATIONAL JOURNAL OF CURRENT ADVANCED RESEARCH, 2023

Background: Convolvulus Pluricaulis (CP) is a well-known ethnopharmacological herb in Ayurveda an... more Background: Convolvulus Pluricaulis (CP) is a well-known ethnopharmacological herb in Ayurveda and exhibits a comprehensive range of therapeutic potential. Objective: The main objective of this research was to study the phytochemical profile of ethanolic extract of CP by Gas Chromatography Liquid Chromatography (GC-MS) analysis and to review the pharmacological activities associated with the compounds identified in this study from the literature. Materials and Methods: Ethanolic extract of CP was prepared by soxhlet extraction and the extract was subjected to GC-MS analysis (Agilent 7890A GC System) for chemical characterization of the extract. Results: The constituents were analysed by matching mass spectra with MS libraries. Total 19 different compounds were identified for CP extract belonging to different chemical groups like fatty acids, alkaloid, triterpenoid, diterpenoids, tetracycline antibiotic, cardiac glycoside and long chain aldehyde chemical group. The constituents of pharmacological significance include demeclocycline; n-hexadecanoic acid; hexadecanoic acid, ethyl ester; phytol; squalene; digitoxin; ethyl iso-allocholate; 9,12octadecadienoic acid; 9,12,15-octadecatrienoic acid; Octadecanal 2-bromo; pentadecanoic acid, 14-methyl, methyl ester and 9,12,15-octadecatrienoic acid, 2-phenyl-1,3-dioxan-5-yl ester. Conclusion: The GC-MS analysis was successful in identifying the pharmacologically important phytochemicals and the eluted compounds could provide the researchers to work with different pharmacological activities related models and studies.

International Journal of Current Advance research , 2010

The plant is well known in the form of the herb as natural medicine. It aids in enhancing and str... more The plant is well known in the form of the herb as natural medicine. It aids in enhancing and strengthening memory and also prevents illness progression. In addition, it provides strength and immunity to the body. (Jalw B, Dahiya J, Khokhara S et al., 2016). believed to promote longevity, improve complexion and restore nervous functions, and is found to be alternative tonic, and bitter. It is known effectively in the

International Journal of Basic & Clinical Pharmacology, Dec 23, 2017

Medicinal plants having ethnopharmacological relevance are been used since decades for treatment ... more Medicinal plants having ethnopharmacological relevance are been used since decades for treatment due to their phytoconstituents. Synthetic molecules pose multiple problems even leading to death. 1 Witnessing the thrust area of immunology as the emerging field of research, the team has propagated the work in this paper. Secondary immunodeficiencies are consequences caused by cytotoxic drugs, malnutrition, infections and metabolic disorders resulting in specific and nonspecific immunity e.g. cancer, AIDS etc. 2 Cancer is a disease characterized by uncontrolled multiplication of normal cells. As per Indian population census, rate of mortality due to cancer was alarming with about 8,06,000 existing cases by the end of last century. Developed countries have now changed their paradigm towards exploring herbal medicine. 3 As one-third population actually lack access to essential ABSTRACT Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques. Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens. The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay. Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay. Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

Pharmacognosy Journal, 2016

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Ob... more Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed-Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10 9) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

Pharmacognosy Journal, Jul 1, 2016

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Ob... more Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed-Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10 9) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

International journal of biology, pharmacy and allied sciences, Oct 14, 2023

Background: For drug discovery and development of novel therapeutic agents against diseases, unde... more Background: For drug discovery and development of novel therapeutic agents against diseases, understanding phytochemistry in plants is very crucial. Objective: The aim of this research was to identify the phytochemical profile of ethanolic extract of Convolvulus pluricaulis (CP), Michelia champaca (MC), and Chromolaena odorata (CO) by using LC-MS technique. Materials and Methods: The extract of selected plants by soxhlet extraction technique was prepared by using ethanol as a solvent. The extract was subjected to LC-MS analysis and the identified constituents were further reviewed for their pharmacological potentials. Results: LC-MS findings has provided significant contribution in identification of phytoconstituents in herbs belonging to a) phenolic compounds-flavonoids, phenylpropanoids; b) alkaloids-amide, proline and quinoline and c) pentacyclic triterpenes.

World Journal of Pharmaceutical Science and Research, 2023

Background: Cancer immunology is a rapidly growing field of research that studies interactions be... more Background: Cancer immunology is a rapidly growing field of research that studies interactions between the immune system and cancer cells. The selected plants have proven their immunomodulatory effects via cellular and humoral mediated immunity in animal models in our previous research work. Objective: To screen the ethanolic extracts of Convolvulus pluricaulis (CP), Michelia champaca (MC), Chromolaena odorata (CO) and combination of above three plants (CP+MC+CO) for in-vitro anticancer activity using breast and colon human cancer cell lines-MCF-7 and HT-29. Methods and materials: The extracts of selected plants (whole plant extract) were prepared by using maceration technique using ethanol as a solvent. The extracts were subjected for in-vitro anticancer potential using sulforhodamine B (SRB) assay against breast and colon cancer cell lines at concentrations of 10, 20, 40 and 80 µg/ml. The standard drug Adriamycin was used. Results: All the extracts inhibited percent control growth in dose dependent manner for both breast and colon cancer cell line. GI50 (Concentration of drug causing 50% inhibition of cell growth) for breast cancer cell line in CP extract was more than 80 µg/ml, and in MC, CO, and CP+MC+CO extract it was found to be 77.6, 49.5 and 78.2 µg/ml. GI50 for colon cancer cell line in all the extracts was found to be more than 80 µg/ml. Optimization in experimental conditions or turning extracts into novel drug delivery systems can enhance the medicinal effects of the plants and can reduce the GI50 concentration. Conclusion: Optimization in experimental conditions or turning extracts into novel drug delivery systems can enhance the medicinal effects of the plants and can reduce the GI50 concentration to desired standard level considering the phenolic/flavonoid richness of these plants.

International Journal of Biology, Pharmacy and Allied Scinces, 2023

Background: For drug discovery and development of novel therapeutic agents against diseases, unde... more Background: For drug discovery and development of novel therapeutic agents against diseases, understanding phytochemistry in plants is very crucial. Objective: The aim of this research was to identify the phytochemical profile of ethanolic extract of Convolvulus pluricaulis (CP), Michelia champaca (MC), and Chromolaena odorata (CO) by using LC-MS technique. Materials and Methods: The extract of selected plants by soxhlet extraction technique was prepared by using ethanol as a solvent. The extract was subjected to LC-MS analysis and the identified constituents were further reviewed for their pharmacological potentials. Results: LC-MS findings has provided significant contribution in identification of phytoconstituents in herbs belonging to a) phenolic compounds-flavonoids, phenylpropanoids; b) alkaloids-amide, proline and quinoline and c) pentacyclic triterpenes.

INTERNATIONAL JOURNAL OF CURRENT ADVANCED RESEARCH, 2023

Background: Convolvulus Pluricaulis (CP) is a well-known ethnopharmacological herb in Ayurveda an... more Background: Convolvulus Pluricaulis (CP) is a well-known ethnopharmacological herb in Ayurveda and exhibits a comprehensive range of therapeutic potential. Objective: The main objective of this research was to study the phytochemical profile of ethanolic extract of CP by Gas Chromatography Liquid Chromatography (GC-MS) analysis and to review the pharmacological activities associated with the compounds identified in this study from the literature. Materials and Methods: Ethanolic extract of CP was prepared by soxhlet extraction and the extract was subjected to GC-MS analysis (Agilent 7890A GC System) for chemical characterization of the extract. Results: The constituents were analysed by matching mass spectra with MS libraries. Total 19 different compounds were identified for CP extract belonging to different chemical groups like fatty acids, alkaloid, triterpenoid, diterpenoids, tetracycline antibiotic, cardiac glycoside and long chain aldehyde chemical group. The constituents of pharmacological significance include demeclocycline; n-hexadecanoic acid; hexadecanoic acid, ethyl ester; phytol; squalene; digitoxin; ethyl iso-allocholate; 9,12octadecadienoic acid; 9,12,15-octadecatrienoic acid; Octadecanal 2-bromo; pentadecanoic acid, 14-methyl, methyl ester and 9,12,15-octadecatrienoic acid, 2-phenyl-1,3-dioxan-5-yl ester. Conclusion: The GC-MS analysis was successful in identifying the pharmacologically important phytochemicals and the eluted compounds could provide the researchers to work with different pharmacological activities related models and studies.

International Journal of Current Advance research , 2010

The plant is well known in the form of the herb as natural medicine. It aids in enhancing and str... more The plant is well known in the form of the herb as natural medicine. It aids in enhancing and strengthening memory and also prevents illness progression. In addition, it provides strength and immunity to the body. (Jalw B, Dahiya J, Khokhara S et al., 2016). believed to promote longevity, improve complexion and restore nervous functions, and is found to be alternative tonic, and bitter. It is known effectively in the

International Journal of Basic & Clinical Pharmacology, Dec 23, 2017

Medicinal plants having ethnopharmacological relevance are been used since decades for treatment ... more Medicinal plants having ethnopharmacological relevance are been used since decades for treatment due to their phytoconstituents. Synthetic molecules pose multiple problems even leading to death. 1 Witnessing the thrust area of immunology as the emerging field of research, the team has propagated the work in this paper. Secondary immunodeficiencies are consequences caused by cytotoxic drugs, malnutrition, infections and metabolic disorders resulting in specific and nonspecific immunity e.g. cancer, AIDS etc. 2 Cancer is a disease characterized by uncontrolled multiplication of normal cells. As per Indian population census, rate of mortality due to cancer was alarming with about 8,06,000 existing cases by the end of last century. Developed countries have now changed their paradigm towards exploring herbal medicine. 3 As one-third population actually lack access to essential ABSTRACT Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques. Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens. The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay. Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay. Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

Pharmacognosy Journal, 2016

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Ob... more Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed-Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10 9) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

Pharmacognosy Journal, Jul 1, 2016

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Ob... more Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed-Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10 9) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.