Manolis Liatsis - Academia.edu (original) (raw)

Papers by Manolis Liatsis

Research paper thumbnail of 1093. Multiparametric Investigation Of Spike-Protein Specific T-cell Cytokine Expression Profile In Children With Symptomatic COVID-19 Or Multisystem Inflammatory Syndrome

Open Forum Infectious Diseases

Background Data regarding cell-mediated immunological differences in children across COVID-19 cli... more Background Data regarding cell-mediated immunological differences in children across COVID-19 clinical spectrum are limited. We prospectively investigated Spike-protein specific cellular immunity in children with symptomatic COVID-19 or MIS-C, by single cell cytokine expression profiling. Methods PBMCs from children with MIS-C, symptomatic COVID-19 (one month after hospitalization) and healthy controls were isolated prospectively. Cell suspensions were divided into two quantitatively equal samples (a negative control-unstimulated and a positive-stimulated). Cells stimulation was performed using SARS-CoV-2 Spike antigenic peptides mix (Peptivator SARS-CoV-2 Prot_S). Cells of each sample were stained with fluorochrome monoclonal antibodies against 8 surface markers (CD3, CD4, CD8, CD14, CD19, CD137, CD197, CD45RA) and 6 intracellular markers (IL-4, IL-2, IL-17, IFN-γ, TNF-α, Granzyme B). Viability was assessed by 7AAD. Stained cell preparations were analysed using 13 colour Flow Cytom...

Research paper thumbnail of P650 Integrin expression patterns of patients with IBD treated with vedolizumab

Journal of Crohn's and Colitis

Background Anti-α4β7 integrin monoclinic antibody vedolizumab is an effective treatment for moder... more Background Anti-α4β7 integrin monoclinic antibody vedolizumab is an effective treatment for moderate and severe Ulcerative Colitis and Crohn’s Disease. Occasionally vedolizumab-treated patients experience articular extraintestinal manifestations, in the form of arthralgias, which could be attributed to lymphocyte tracking modification caused by vedolizumab. This study aims to describe α4β7 andα4β1 integrin expression on circulating lymphocytes and the effect of vedolizumab on integrin expression, as well asto identify expression patterns associated with arthralgias under vedolizumab. Methods A cross-sectional study was conducted. Peripheral blood was collected in EDTA-coated bottles before a scheduled drug administration. Expression of α4β7 and α4β1 integrins on CD4+T, CD8+T, B and plasma cells was studied by flow cytometry (DX Flex, BC), using CD3, CD4, CD8, CD19,CD138, CD49d (α4), β7, CD29 (β1). Kaluza C software and SPSS23 were used for analysis. Results In total, 40 IBD patients...

Research paper thumbnail of 131 Basophil activation test for the early diagnosis of allergic bronchopulmonary aspergillosis (ABPA)

Journal of Cystic Fibrosis, 2007

Background: supplementation with n-3 polyunsaturated fatty acids (PUFA) leads to production of le... more Background: supplementation with n-3 polyunsaturated fatty acids (PUFA) leads to production of leukotriene (LT) B5, which is biologically less active than LTB4. LTB4 is important chemoattractant in neutrophilic inflammation in CF lung disease. Methods: nine F508del homozygous CF patients (5 females) aged 25.6±3.3 (mean±SD) years were treated for 6 weeks with n-3 PUFA (dosis corresponding to 1.3% of energy intake). Exhaled breath condensates (EBC) were collected before and after treatment. pH and LTB4 concentration (pg/ml) were measured in EBC samples. Results: pH was increased (from 6.08±0.39 to 6.32±0.18; p = 0.013) and LTB4 concentration was decreased (from 227.3±195.0 to 134.9±67.3 pg/ml; p = 0.075) in EBC after n-3 PUFA treatment. Conclusion: LTB4 concentration in EBC tend to be lower after n-3 PUFA treatment and EBC is significantly less acidified, which may reflect less intense neutrophilic inflammation.

Research paper thumbnail of Chronic Granulomatous Disease: A 25-Year Patient Registry Based on a Multistep Diagnostic Procedure, from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2013

Chronic Granulomatous Disease (CGD) is an uncommon primary immunodeficiency caused by the absence... more Chronic Granulomatous Disease (CGD) is an uncommon primary immunodeficiency caused by the absence or dysfunction of one of NADPH oxidase subunits, with heterogeneous genetic aetiologies. The aim of this study was the CGD patient registry in Greece, the identification of the responsible genotype and the potential correlation with the patient's clinical phenotype. Medical charts of 24 CGD patients, investigated by NBT test or DHR for NADPH oxidase activity, Western blot analysis for NADPH oxidase component expression and DNA sequencing (pyro- and cycle sequencing) for mutation analysis, were reviewed. All patients, but one, were classified into the different types of CGD. Sixteen from 14 unrelated families had X-linked CGD (66.7 %), four patients had mutations in the NCF1 gene (16.7 %), and three, from two unrelated families, had mutations in NCF2 (12.5 %). Fifteen mutations were detected in the CYBB gene, including nonsense (53.8 %), splice site (30.8 %) and missense mutations (7.7 %), and deletions (7.7 %). Two novel mutations were identified; one in CYBB and one in NCF1. Carrier detection for X-CGD revealed that the de novo mutation rate was about 7 %. Prenatal diagnosis identified one affected male in three male fetuses tested. In both the X-linked and the autosomal recessive (AR-CGD) group, the gastrointestinal and respiratory manifestations were more common, followed by lympadenopathy in X-CGD and skin infections in the AR-CGD group. The patients with a mutation in CYBB had a wider variability of clinical manifestations and earlier diagnosis (4.6 years) compared to the AR-CGD group (12.9 years). The incidence of CGD in Greece is estimated at 0.90 (95 % CI 0.89-0.91) per 100,000 live births for the last decade.

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of Greek patients with digeorge syndrome cytogenetics and immunological studies

Molecular Immunology, 1998

Research paper thumbnail of Listeria Multiple Brain Abscesses in an Immunocompromised 12-Year-Old Greek Boy

Infectious Diseases in Clinical Practice, 2007

... Address correspondence and reprint requests to Sophia Delicou, MD, 21-21 Embedokleous str, Pa... more ... Address correspondence and reprint requests to Sophia Delicou, MD, 21-21 Embedokleous str, Pagrati, 116.36, Athens, Greece. E-mail: sophiadelicou@gmail. com. Collapse Box Abstract. ... Hauf N, Goebel W, Fiedler F, et al. ...

Research paper thumbnail of Serum immunoglobulin G subclasses in healthy infants and children in Greece

European journal of epidemiology, 1997

Serum concentrations of immunoglobulin IgG1, IgG2, IgG3 and IgG4 were determined by radial immuno... more Serum concentrations of immunoglobulin IgG1, IgG2, IgG3 and IgG4 were determined by radial immunodiffusion in a sample of 414 healthy Greek children, who were admitted to the major Teaching Hospital for Children in Athens for minor surgical operations. Statistical analysis was performed by multiple regression after logarithmic transformation of the immunoglobulin values. There was a statistically significant increase of IgG3 with age, whereas IgG1, IgG2 and IgG4 levels reached a turning point at the age of five years. Older than 5 years male children were found to have marginally higher IgG4 levels than females. Low socioeconomic class was positively and significantly associated (9% increase) with IgG1 levels only among the older age group. History of frequent infections was associated with a 16% increase of IgG1 levels in the younger (less than 5 years) (p = 0.01) and with a 47% increase of IgG4 among the older age group (p = 0.03). Atopic history was associated with a 16% increase...

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of ANTINUCLEAR ANTIBODIES IN GREEK PAEDIATRIC POPULATION 64

Research paper thumbnail of ANTIBODY RESPONSE TO CAPSULAR POLYSACCHARIDES OF STREPTOCOCCUS PNEUMONIAE IN SPLENECTOMIZED CHILDREN WITH B-THALASSEMIA. PERSISTENSE AND RESPONSE TO REVACCINATION. 63

Research paper thumbnail of Association of allergic sensitization with infectious diseases burden in Roma and non-Roma children

Pediatric Allergy and Immunology, 2011

To cite this article: Michos A, Terzidis A, Kanariou M, Kalampoki V, Koilia C, Giannaki M, Liatsi... more To cite this article: Michos A, Terzidis A, Kanariou M, Kalampoki V, Koilia C, Giannaki M, Liatsis M, Pangalis A, Petridou E. Association of allergic sensitization with infectious diseases burden in Roma and non-Roma children. Pediatr Allergy Immunol 2011; 22: 243-248.

Research paper thumbnail of Children with frequent infections: A proposal for a stepwise assessment and investigation of the immune system

Pediatric Allergy and Immunology, 2009

Research paper thumbnail of Age patterns of immunoglobulins G, A & M in healthy children and the influence of breast feeding and vaccination status

Pediatric Allergy and Immunology, 1995

Research paper thumbnail of Experience on bone marrow transplantation (BMT) for primary immunodeficiencies (PID) in ?Aghia Sophia? children's hospital

Molecular Immunology, 1998

Research paper thumbnail of 230 ABPA is not rare in CF patients under 6 years old: Epidemiological data in Greek CF patients for the period 1990–2005

Journal of Cystic Fibrosis, 2006

Research paper thumbnail of Factors influencing serum immunoglobulin E levels in Greek children

Allergy, 1995

Serum levels of immunoglobulin E (IgE) were determined by enzyme immunoassay in 414 Greek infants... more Serum levels of immunoglobulin E (IgE) were determined by enzyme immunoassay in 414 Greek infants and children of both sexes, 1 month to 14 years old. The children were admitted to the "Aghia Sophia" Teaching Hospital for Children (Athens, Greece) for surgical corrections of minor anatomic abnormalities, but they were otherwise healthy. Statistical analysis was performed through multiple regression after logarithmic transformation of the immunoglobulin values. IgE levels increased significantly by about 80% per year up to the age of 5 years, without noticeable impact of age on these levels thereafter. History of allergic disease showed a significant positive association with serum levels of IgE in both younger (less than 5 years) and older children. History of frequent infections was positively associated with IgE levels, although the relation was statistically significant only in the older age group, IgE levels in Greek children appear to be higher than the corresponding levels of children living in northern Europe, but much lower than those of children in Southeast Asia, a fact that may reflect different exposure level during childhood to infections known to influence IgE levels.

Research paper thumbnail of Erratum to: Chronic Granulomatous Disease: A 25-Year Patient Registry Based on a Multistep Diagnostic Procedure, from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Research paper thumbnail of 1093. Multiparametric Investigation Of Spike-Protein Specific T-cell Cytokine Expression Profile In Children With Symptomatic COVID-19 Or Multisystem Inflammatory Syndrome

Open Forum Infectious Diseases

Background Data regarding cell-mediated immunological differences in children across COVID-19 cli... more Background Data regarding cell-mediated immunological differences in children across COVID-19 clinical spectrum are limited. We prospectively investigated Spike-protein specific cellular immunity in children with symptomatic COVID-19 or MIS-C, by single cell cytokine expression profiling. Methods PBMCs from children with MIS-C, symptomatic COVID-19 (one month after hospitalization) and healthy controls were isolated prospectively. Cell suspensions were divided into two quantitatively equal samples (a negative control-unstimulated and a positive-stimulated). Cells stimulation was performed using SARS-CoV-2 Spike antigenic peptides mix (Peptivator SARS-CoV-2 Prot_S). Cells of each sample were stained with fluorochrome monoclonal antibodies against 8 surface markers (CD3, CD4, CD8, CD14, CD19, CD137, CD197, CD45RA) and 6 intracellular markers (IL-4, IL-2, IL-17, IFN-γ, TNF-α, Granzyme B). Viability was assessed by 7AAD. Stained cell preparations were analysed using 13 colour Flow Cytom...

Research paper thumbnail of P650 Integrin expression patterns of patients with IBD treated with vedolizumab

Journal of Crohn's and Colitis

Background Anti-α4β7 integrin monoclinic antibody vedolizumab is an effective treatment for moder... more Background Anti-α4β7 integrin monoclinic antibody vedolizumab is an effective treatment for moderate and severe Ulcerative Colitis and Crohn’s Disease. Occasionally vedolizumab-treated patients experience articular extraintestinal manifestations, in the form of arthralgias, which could be attributed to lymphocyte tracking modification caused by vedolizumab. This study aims to describe α4β7 andα4β1 integrin expression on circulating lymphocytes and the effect of vedolizumab on integrin expression, as well asto identify expression patterns associated with arthralgias under vedolizumab. Methods A cross-sectional study was conducted. Peripheral blood was collected in EDTA-coated bottles before a scheduled drug administration. Expression of α4β7 and α4β1 integrins on CD4+T, CD8+T, B and plasma cells was studied by flow cytometry (DX Flex, BC), using CD3, CD4, CD8, CD19,CD138, CD49d (α4), β7, CD29 (β1). Kaluza C software and SPSS23 were used for analysis. Results In total, 40 IBD patients...

Research paper thumbnail of 131 Basophil activation test for the early diagnosis of allergic bronchopulmonary aspergillosis (ABPA)

Journal of Cystic Fibrosis, 2007

Background: supplementation with n-3 polyunsaturated fatty acids (PUFA) leads to production of le... more Background: supplementation with n-3 polyunsaturated fatty acids (PUFA) leads to production of leukotriene (LT) B5, which is biologically less active than LTB4. LTB4 is important chemoattractant in neutrophilic inflammation in CF lung disease. Methods: nine F508del homozygous CF patients (5 females) aged 25.6±3.3 (mean±SD) years were treated for 6 weeks with n-3 PUFA (dosis corresponding to 1.3% of energy intake). Exhaled breath condensates (EBC) were collected before and after treatment. pH and LTB4 concentration (pg/ml) were measured in EBC samples. Results: pH was increased (from 6.08±0.39 to 6.32±0.18; p = 0.013) and LTB4 concentration was decreased (from 227.3±195.0 to 134.9±67.3 pg/ml; p = 0.075) in EBC after n-3 PUFA treatment. Conclusion: LTB4 concentration in EBC tend to be lower after n-3 PUFA treatment and EBC is significantly less acidified, which may reflect less intense neutrophilic inflammation.

Research paper thumbnail of Chronic Granulomatous Disease: A 25-Year Patient Registry Based on a Multistep Diagnostic Procedure, from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2013

Chronic Granulomatous Disease (CGD) is an uncommon primary immunodeficiency caused by the absence... more Chronic Granulomatous Disease (CGD) is an uncommon primary immunodeficiency caused by the absence or dysfunction of one of NADPH oxidase subunits, with heterogeneous genetic aetiologies. The aim of this study was the CGD patient registry in Greece, the identification of the responsible genotype and the potential correlation with the patient's clinical phenotype. Medical charts of 24 CGD patients, investigated by NBT test or DHR for NADPH oxidase activity, Western blot analysis for NADPH oxidase component expression and DNA sequencing (pyro- and cycle sequencing) for mutation analysis, were reviewed. All patients, but one, were classified into the different types of CGD. Sixteen from 14 unrelated families had X-linked CGD (66.7 %), four patients had mutations in the NCF1 gene (16.7 %), and three, from two unrelated families, had mutations in NCF2 (12.5 %). Fifteen mutations were detected in the CYBB gene, including nonsense (53.8 %), splice site (30.8 %) and missense mutations (7.7 %), and deletions (7.7 %). Two novel mutations were identified; one in CYBB and one in NCF1. Carrier detection for X-CGD revealed that the de novo mutation rate was about 7 %. Prenatal diagnosis identified one affected male in three male fetuses tested. In both the X-linked and the autosomal recessive (AR-CGD) group, the gastrointestinal and respiratory manifestations were more common, followed by lympadenopathy in X-CGD and skin infections in the AR-CGD group. The patients with a mutation in CYBB had a wider variability of clinical manifestations and earlier diagnosis (4.6 years) compared to the AR-CGD group (12.9 years). The incidence of CGD in Greece is estimated at 0.90 (95 % CI 0.89-0.91) per 100,000 live births for the last decade.

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of Greek patients with digeorge syndrome cytogenetics and immunological studies

Molecular Immunology, 1998

Research paper thumbnail of Listeria Multiple Brain Abscesses in an Immunocompromised 12-Year-Old Greek Boy

Infectious Diseases in Clinical Practice, 2007

... Address correspondence and reprint requests to Sophia Delicou, MD, 21-21 Embedokleous str, Pa... more ... Address correspondence and reprint requests to Sophia Delicou, MD, 21-21 Embedokleous str, Pagrati, 116.36, Athens, Greece. E-mail: sophiadelicou@gmail. com. Collapse Box Abstract. ... Hauf N, Goebel W, Fiedler F, et al. ...

Research paper thumbnail of Serum immunoglobulin G subclasses in healthy infants and children in Greece

European journal of epidemiology, 1997

Serum concentrations of immunoglobulin IgG1, IgG2, IgG3 and IgG4 were determined by radial immuno... more Serum concentrations of immunoglobulin IgG1, IgG2, IgG3 and IgG4 were determined by radial immunodiffusion in a sample of 414 healthy Greek children, who were admitted to the major Teaching Hospital for Children in Athens for minor surgical operations. Statistical analysis was performed by multiple regression after logarithmic transformation of the immunoglobulin values. There was a statistically significant increase of IgG3 with age, whereas IgG1, IgG2 and IgG4 levels reached a turning point at the age of five years. Older than 5 years male children were found to have marginally higher IgG4 levels than females. Low socioeconomic class was positively and significantly associated (9% increase) with IgG1 levels only among the older age group. History of frequent infections was associated with a 16% increase of IgG1 levels in the younger (less than 5 years) (p = 0.01) and with a 47% increase of IgG4 among the older age group (p = 0.03). Atopic history was associated with a 16% increase...

Research paper thumbnail of Primary Immunodeficiency Diseases: A 30-year Patient Registry from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014

Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with importan... more Primary Immunodeficiencies (PID) represent a group of heterogeneous immune diseases with important biological significance. We reviewed the records of children diagnosed with PID in the Referral Center for PID in our country in order to describe the epidemiological, clinical and laboratory characteristics of immunodeficient patients. During a 30-year period, 147 patients (101 males, 68.7 %), with a mean age of 6.5 years at the time of diagnosis, were diagnosed with PID. The most prevalent diagnoses of PID were: "Combined Immunodeficiency" in 46 (31.3 %) patients, "Well-defined immunodeficiency syndrome" in 35 (23.1 %) patients, "Predominantly antibody deficiency" in 30 (20.4 %) patients and "Congenital defect of phagocyte function or both" in 28 (19 %) patients. There was a higher prevalence of males with "Combined immunodeficiency" (p < 0.033) and "Predominantly antibody deficiency" (p < 0.02) compared to females. The median age of children at the onset of symptoms and at the time of diagnosis was 0.5y (IQR: 0.1-2.5) and 2y (IQR: 0.6-7.2), respectively. The median diagnostic delay was 0.9y (IQR: 0.2-4.8). This period was shorter for patients with "Combined immunodeficiency" [median 0.3y (IQR: 0.1-1)], and longer for those with "Predominantly antibody deficiency" [median 3.2y (IQR: 0.2-5.9) or "Disease of immune dysregulation" [median 3.2y (IQR: 0.1-6.6)]. Comparing the rates in our population with those of the European Registry (ESID), the rates of "Combined immunodeficiencies", "Well-defined syndromes" and "Congenital birth defects and/or function of phagocytes" were significantly higher in this study (p <0,001). PID registry analysis improves knowledge in the field of Immunology and enhances awareness, early detection, diagnosis, and management of this rare but significant group of diseases.

Research paper thumbnail of ANTINUCLEAR ANTIBODIES IN GREEK PAEDIATRIC POPULATION 64

Research paper thumbnail of ANTIBODY RESPONSE TO CAPSULAR POLYSACCHARIDES OF STREPTOCOCCUS PNEUMONIAE IN SPLENECTOMIZED CHILDREN WITH B-THALASSEMIA. PERSISTENSE AND RESPONSE TO REVACCINATION. 63

Research paper thumbnail of Association of allergic sensitization with infectious diseases burden in Roma and non-Roma children

Pediatric Allergy and Immunology, 2011

To cite this article: Michos A, Terzidis A, Kanariou M, Kalampoki V, Koilia C, Giannaki M, Liatsi... more To cite this article: Michos A, Terzidis A, Kanariou M, Kalampoki V, Koilia C, Giannaki M, Liatsis M, Pangalis A, Petridou E. Association of allergic sensitization with infectious diseases burden in Roma and non-Roma children. Pediatr Allergy Immunol 2011; 22: 243-248.

Research paper thumbnail of Children with frequent infections: A proposal for a stepwise assessment and investigation of the immune system

Pediatric Allergy and Immunology, 2009

Research paper thumbnail of Age patterns of immunoglobulins G, A & M in healthy children and the influence of breast feeding and vaccination status

Pediatric Allergy and Immunology, 1995

Research paper thumbnail of Experience on bone marrow transplantation (BMT) for primary immunodeficiencies (PID) in ?Aghia Sophia? children's hospital

Molecular Immunology, 1998

Research paper thumbnail of 230 ABPA is not rare in CF patients under 6 years old: Epidemiological data in Greek CF patients for the period 1990–2005

Journal of Cystic Fibrosis, 2006

Research paper thumbnail of Factors influencing serum immunoglobulin E levels in Greek children

Allergy, 1995

Serum levels of immunoglobulin E (IgE) were determined by enzyme immunoassay in 414 Greek infants... more Serum levels of immunoglobulin E (IgE) were determined by enzyme immunoassay in 414 Greek infants and children of both sexes, 1 month to 14 years old. The children were admitted to the "Aghia Sophia" Teaching Hospital for Children (Athens, Greece) for surgical corrections of minor anatomic abnormalities, but they were otherwise healthy. Statistical analysis was performed through multiple regression after logarithmic transformation of the immunoglobulin values. IgE levels increased significantly by about 80% per year up to the age of 5 years, without noticeable impact of age on these levels thereafter. History of allergic disease showed a significant positive association with serum levels of IgE in both younger (less than 5 years) and older children. History of frequent infections was positively associated with IgE levels, although the relation was statistically significant only in the older age group, IgE levels in Greek children appear to be higher than the corresponding levels of children living in northern Europe, but much lower than those of children in Southeast Asia, a fact that may reflect different exposure level during childhood to infections known to influence IgE levels.

Research paper thumbnail of Erratum to: Chronic Granulomatous Disease: A 25-Year Patient Registry Based on a Multistep Diagnostic Procedure, from the Referral Center for Primary Immunodeficiencies in Greece

Journal of Clinical Immunology, 2014