Wafaa Mansour - Academia.edu (original) (raw)

Papers by Wafaa Mansour

This is a supplementary data for a manuscript submitted to JAP

Author contributions: Ghanem LY, Dahmen U and Dirsch O were responsible for construction of the p... more Author contributions: Ghanem LY, Dahmen U and Dirsch O were responsible for construction of the plan and design of work, discussion of results, writing the manuscript and financial support; Nosseir MMF for acquisition, interpretation, analysis and discussion of histopathological results, providing histopa thological figures and revising the text critically; Mahmoud SS performed parasitological investigations and collected data; and Mansour WAF helped in discussion of the results and perfor mance of laboratory investigations.

Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELIS... more Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/ secretory antigens in both serum and stool

Experimental and Clinical Transplantation, 2021

OBJECTIVES Hepatocellular carcinoma is the fourth leading cause of cancer deaths in the world. Co... more OBJECTIVES Hepatocellular carcinoma is the fourth leading cause of cancer deaths in the world. Conventional methods of cancer therapy are either invasive or have undesirable side effects. Therefore, exploring new therapeutic strategies to control the progression of hepatocellular carcinoma, such as cell-based therapies, is a key issue for prolonging patient survival. In this study, we aimed to evaluate tumor suppressive effects of mesenchymal stem cells on the in vivo progression of hepatocellular carcinoma in murine model. MATERIALS AND METHODS Hepatocellular carcinoma was induced in 40 rats with diethylnitrosamine. Rats were divided into 4 groups: 1 group injected with diethylnitrosamine only, 1 group injected with diethylnitrosamine and 1 dose of rat bone marrowderived mesenchymal stem cells, 1 group injected with diethylnitrosamine and 2 doses of rat bone marrowderived mesenchymal stem cells, and 1 group was injected with diethylnitrosamine and 3 doses of rat bone marrow-derived mesenchymal stem cells. Rats were killed after 1 month of dose 3. Liver specimens were histopathologically examined, and serum samples were examined for liver function and cytokines. RESULTS Histopathological examination revealed that mesenchymal stem cell transplant induced liver regeneration. It also improved liver function as revealed by decreased levels of alanine and aspartate aminotransferase. Mesenchymal stem cells also repaired the immunopathology of the liver environment, as it decreased levels of interleukin 2 and 10, tumor necrosis factor α, and interferon γ. CONCLUSIONS Mesenchymal stem cell infusion significantly enhanced hepatic structure and function of livers in a rat hepatocellular carcinoma model.

The Japan Society of Applied Physics, 2013

Journal of Global Pharma Technology, 2018

The aim of this study is investigate the polymorphisms of Candida guilliermondii based on ITS4 ge... more The aim of this study is investigate the polymorphisms of Candida guilliermondii based on ITS4 gene sequencing. To achieve this goal, samples were collected from patients with dermal infection symptoms, were applied for microbiological analysis using SDA media DNA was isolated from Candida guilliermondii and the ITS4 gene were amplified by using specific primer, then sequencing of nucleic acid of genes was performed by machine is AB13730XL, Applied Biosystem, Macro gen company, the DNA sequencing results of flank sense of ITS4 gene from 5 strains of Candida guilliermondii was found to be compatible 99% and score 1517 and expect 0.0 of the ITS4 gene of Candida guilliermondii strain BO418 from the Gene Bank, The expected substitution were found after the analysis of the sequences there were 5 Transversion and one transition in the 5 Candida guilliermondii with Sequence ID emb|AJ3tf10144.1| location at range of nucleotide from 5128 to 5408, compared with data obtained from Gene B...

O presente artigo e fruto de um projeto intitulado como "Direito em Atividade" desenvol... more O presente artigo e fruto de um projeto intitulado como "Direito em Atividade" desenvolvido pelo curso de Direito da FACIG no segundo semestre do ano de 2017, que teve como objetivo a discussao de temas relevantes para a comunidade academica. O presente artigo, realizado pela colaboracao da liga academica "Direitoria", tendo como objetivo a analise da legislacao acerca da condicao analoga a de escravo no Brasil, percorrendo um contexto historico, e nao obstante, sua observacao perante o caso concreto da idosa que foi mantida como escrava no estado de Minas Gerais. Sendo realizada tambem uma analise utilizando dados para validar de forma estatistica as afirmacoes dispostas. Deste modo, o trabalho realiza uma analise predominantemente qualitativa, atraves do metodo hipotetico-dedutivo, por meio da revisao bibliografica, bem como dos construtos legislativos.

Journal of the American College of Cardiology, 2021

Coal Processing and Comprehensive Utilization, 2002

With Datong coal as feedstock,addition of appropriate chemical solution and employing Stepple fur... more With Datong coal as feedstock,addition of appropriate chemical solution and employing Stepple furnace,obtained was an activated carbon with predominant macro and meso pore and improved absorbability.

Acute myelogenous leukemia (AML) is a class of malignancy derived from the myeloid line of aberra... more Acute myelogenous leukemia (AML) is a class of malignancy derived from the myeloid line of aberrant progenitor/stem cells from bone marrow. AML is incurable and the five year rate of the event free survival (EFS) remains less than 20% even with the intensive chemotherapy in combination with autologous hematopoietic stem cell transplantation. Therefore, it is urgent to find novel drugs to improve the outcome of AML patients. Paris forrestii (Takht.) H. Li is a traditional herb medicine specifically produced in the Yu9nan and Tibet regions of China and it is widely used for the inflammation and coagulation among local people. In the study to identify natural products for cancer treatment, we isolated and evaluated the active ingredients from Paris forrestii (Takht.) H. Li and found that PCT3, a combination of steroidal saponins, displayed potent anti-AML activity. The high performance liquid chromatography/mass spectrometry (HPLC/MS) assay isolated and identified 10 steroidal saponins...

21 Abstract: A simple and rapid dot-immunogold filtration assay (DIGFA) technique was used in the... more 21 Abstract: A simple and rapid dot-immunogold filtration assay (DIGFA) technique was used in the present study for rapid detection of antigenaemia in the sera of schistosomiasis patients, using a pair of monoclonal antibodies, the first as antigen capture and the second labeled with red color colloidal gold as antigen detector. Sandwich - ELISA technique, dot-ELISA and dipstick ELISA were used in comparison with the DIGFA technique to evaluate its sensitivity and specificity. The results showed that the percentage positivity of antigenaemia in scistosomiasis patients' sera, using sandwich-ELISA technique was 100%, while in dot-ELISA and dipstick-ELISA the positivity rate was 89.7% and 91.2% respectively. The DIGFA technique showed 97.1% positivity, while the sensitivity and specificity of DIGFA were 95.8% and 95% respectively. In sandwich-ELISA, dot-ELISA and dipstick-ELISA the sensitivity was 100%, 90.7% and 91.9% respectively, and the specificity was 96.6%, 91.9% and 91.9% re...

Este artigo e o resultado de um da projeto de extensao realizado entre alunos do curso de Direito... more Este artigo e o resultado de um da projeto de extensao realizado entre alunos do curso de Direito da FACIG, atraves do Nucleo de Pratica Juridica (NPJ) e do Conselho de ensino, pesquisa e extensao (CONSEPE) ambos da FACIG com pequenos e medios produtores e trabalhadores rurais na Comunidade do Coqueiro Rural de Manhuacu/MG, objetivando levar conhecimento juridico aos referidos agricultores e contribuir com a formacao dos alunos do curso de direito da FACIG, preparando-os para melhor atender as demandas da comunidade rural. Como metodologia de trabalho foram utilizadas reunioes e apresentacoes do tema com a comunidade, para que essas possam expressar suas duvidas, e que fosse possivel levar o conhecimento juridico adequado aos produtores, esclarecendo seus direitos e deveres, para que estes nao deixassem de conquistar seus direitos e descumprir com seus deveres, devido a falta de informacao. Ao final, a comunidade pode discutir sobre a falta de informacao e a importância de levar con...

Journal of Biotechnology Research Center, 2017

The present study aimed to identification of P. vanoranjei based on microscopical and cultural me... more The present study aimed to identification of P. vanoranjei based on microscopical and cultural method as a new record isolate in Iraq. Penicillium vanoranjei is a new species in Iraq that produce an orang production pigment after compelet growth on the culure medium. Location, the spesmen collected from women patient admitted to Al Jimhory Hospital in Samaraa city and lived in Al Jilam township during December/ /2016 in Salah Al Deen Governorate- Samaraa City. Morphological and microscopichal charecterstic appeared that the top view of the culture slightly raised in the center; mycelia was white near the margins, the reversed view appeared Dark brown. Microscopic examination illustrates that the Conidiophores were monoverticillate (unbranched) Penicillium vanoranjei .

This is a supplementary data for a manuscript submitted to JAP

Author contributions: Ghanem LY, Dahmen U and Dirsch O were responsible for construction of the p... more Author contributions: Ghanem LY, Dahmen U and Dirsch O were responsible for construction of the plan and design of work, discussion of results, writing the manuscript and financial support; Nosseir MMF for acquisition, interpretation, analysis and discussion of histopathological results, providing histopa thological figures and revising the text critically; Mahmoud SS performed parasitological investigations and collected data; and Mansour WAF helped in discussion of the results and perfor mance of laboratory investigations.

Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELIS... more Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/ secretory antigens in both serum and stool

Experimental and Clinical Transplantation, 2021

OBJECTIVES Hepatocellular carcinoma is the fourth leading cause of cancer deaths in the world. Co... more OBJECTIVES Hepatocellular carcinoma is the fourth leading cause of cancer deaths in the world. Conventional methods of cancer therapy are either invasive or have undesirable side effects. Therefore, exploring new therapeutic strategies to control the progression of hepatocellular carcinoma, such as cell-based therapies, is a key issue for prolonging patient survival. In this study, we aimed to evaluate tumor suppressive effects of mesenchymal stem cells on the in vivo progression of hepatocellular carcinoma in murine model. MATERIALS AND METHODS Hepatocellular carcinoma was induced in 40 rats with diethylnitrosamine. Rats were divided into 4 groups: 1 group injected with diethylnitrosamine only, 1 group injected with diethylnitrosamine and 1 dose of rat bone marrowderived mesenchymal stem cells, 1 group injected with diethylnitrosamine and 2 doses of rat bone marrowderived mesenchymal stem cells, and 1 group was injected with diethylnitrosamine and 3 doses of rat bone marrow-derived mesenchymal stem cells. Rats were killed after 1 month of dose 3. Liver specimens were histopathologically examined, and serum samples were examined for liver function and cytokines. RESULTS Histopathological examination revealed that mesenchymal stem cell transplant induced liver regeneration. It also improved liver function as revealed by decreased levels of alanine and aspartate aminotransferase. Mesenchymal stem cells also repaired the immunopathology of the liver environment, as it decreased levels of interleukin 2 and 10, tumor necrosis factor α, and interferon γ. CONCLUSIONS Mesenchymal stem cell infusion significantly enhanced hepatic structure and function of livers in a rat hepatocellular carcinoma model.

The Japan Society of Applied Physics, 2013

Journal of Global Pharma Technology, 2018

The aim of this study is investigate the polymorphisms of Candida guilliermondii based on ITS4 ge... more The aim of this study is investigate the polymorphisms of Candida guilliermondii based on ITS4 gene sequencing. To achieve this goal, samples were collected from patients with dermal infection symptoms, were applied for microbiological analysis using SDA media DNA was isolated from Candida guilliermondii and the ITS4 gene were amplified by using specific primer, then sequencing of nucleic acid of genes was performed by machine is AB13730XL, Applied Biosystem, Macro gen company, the DNA sequencing results of flank sense of ITS4 gene from 5 strains of Candida guilliermondii was found to be compatible 99% and score 1517 and expect 0.0 of the ITS4 gene of Candida guilliermondii strain BO418 from the Gene Bank, The expected substitution were found after the analysis of the sequences there were 5 Transversion and one transition in the 5 Candida guilliermondii with Sequence ID emb|AJ3tf10144.1| location at range of nucleotide from 5128 to 5408, compared with data obtained from Gene B...

O presente artigo e fruto de um projeto intitulado como "Direito em Atividade" desenvol... more O presente artigo e fruto de um projeto intitulado como "Direito em Atividade" desenvolvido pelo curso de Direito da FACIG no segundo semestre do ano de 2017, que teve como objetivo a discussao de temas relevantes para a comunidade academica. O presente artigo, realizado pela colaboracao da liga academica "Direitoria", tendo como objetivo a analise da legislacao acerca da condicao analoga a de escravo no Brasil, percorrendo um contexto historico, e nao obstante, sua observacao perante o caso concreto da idosa que foi mantida como escrava no estado de Minas Gerais. Sendo realizada tambem uma analise utilizando dados para validar de forma estatistica as afirmacoes dispostas. Deste modo, o trabalho realiza uma analise predominantemente qualitativa, atraves do metodo hipotetico-dedutivo, por meio da revisao bibliografica, bem como dos construtos legislativos.

Journal of the American College of Cardiology, 2021

Coal Processing and Comprehensive Utilization, 2002

With Datong coal as feedstock,addition of appropriate chemical solution and employing Stepple fur... more With Datong coal as feedstock,addition of appropriate chemical solution and employing Stepple furnace,obtained was an activated carbon with predominant macro and meso pore and improved absorbability.

Acute myelogenous leukemia (AML) is a class of malignancy derived from the myeloid line of aberra... more Acute myelogenous leukemia (AML) is a class of malignancy derived from the myeloid line of aberrant progenitor/stem cells from bone marrow. AML is incurable and the five year rate of the event free survival (EFS) remains less than 20% even with the intensive chemotherapy in combination with autologous hematopoietic stem cell transplantation. Therefore, it is urgent to find novel drugs to improve the outcome of AML patients. Paris forrestii (Takht.) H. Li is a traditional herb medicine specifically produced in the Yu9nan and Tibet regions of China and it is widely used for the inflammation and coagulation among local people. In the study to identify natural products for cancer treatment, we isolated and evaluated the active ingredients from Paris forrestii (Takht.) H. Li and found that PCT3, a combination of steroidal saponins, displayed potent anti-AML activity. The high performance liquid chromatography/mass spectrometry (HPLC/MS) assay isolated and identified 10 steroidal saponins...

21 Abstract: A simple and rapid dot-immunogold filtration assay (DIGFA) technique was used in the... more 21 Abstract: A simple and rapid dot-immunogold filtration assay (DIGFA) technique was used in the present study for rapid detection of antigenaemia in the sera of schistosomiasis patients, using a pair of monoclonal antibodies, the first as antigen capture and the second labeled with red color colloidal gold as antigen detector. Sandwich - ELISA technique, dot-ELISA and dipstick ELISA were used in comparison with the DIGFA technique to evaluate its sensitivity and specificity. The results showed that the percentage positivity of antigenaemia in scistosomiasis patients' sera, using sandwich-ELISA technique was 100%, while in dot-ELISA and dipstick-ELISA the positivity rate was 89.7% and 91.2% respectively. The DIGFA technique showed 97.1% positivity, while the sensitivity and specificity of DIGFA were 95.8% and 95% respectively. In sandwich-ELISA, dot-ELISA and dipstick-ELISA the sensitivity was 100%, 90.7% and 91.9% respectively, and the specificity was 96.6%, 91.9% and 91.9% re...

Este artigo e o resultado de um da projeto de extensao realizado entre alunos do curso de Direito... more Este artigo e o resultado de um da projeto de extensao realizado entre alunos do curso de Direito da FACIG, atraves do Nucleo de Pratica Juridica (NPJ) e do Conselho de ensino, pesquisa e extensao (CONSEPE) ambos da FACIG com pequenos e medios produtores e trabalhadores rurais na Comunidade do Coqueiro Rural de Manhuacu/MG, objetivando levar conhecimento juridico aos referidos agricultores e contribuir com a formacao dos alunos do curso de direito da FACIG, preparando-os para melhor atender as demandas da comunidade rural. Como metodologia de trabalho foram utilizadas reunioes e apresentacoes do tema com a comunidade, para que essas possam expressar suas duvidas, e que fosse possivel levar o conhecimento juridico adequado aos produtores, esclarecendo seus direitos e deveres, para que estes nao deixassem de conquistar seus direitos e descumprir com seus deveres, devido a falta de informacao. Ao final, a comunidade pode discutir sobre a falta de informacao e a importância de levar con...

Journal of Biotechnology Research Center, 2017

The present study aimed to identification of P. vanoranjei based on microscopical and cultural me... more The present study aimed to identification of P. vanoranjei based on microscopical and cultural method as a new record isolate in Iraq. Penicillium vanoranjei is a new species in Iraq that produce an orang production pigment after compelet growth on the culure medium. Location, the spesmen collected from women patient admitted to Al Jimhory Hospital in Samaraa city and lived in Al Jilam township during December/ /2016 in Salah Al Deen Governorate- Samaraa City. Morphological and microscopichal charecterstic appeared that the top view of the culture slightly raised in the center; mycelia was white near the margins, the reversed view appeared Dark brown. Microscopic examination illustrates that the Conidiophores were monoverticillate (unbranched) Penicillium vanoranjei .