Andreas Manz - Academia.edu (original) (raw)

Papers by Andreas Manz

Research paper thumbnail of A Circular AC Electroosmotic Micropump for Chromatographic Applications

Micro Total Analysis Systems 2002, 2002

Flow rates of up to 50 µm s -1 have been successfully achieved in a circular AC electroosmotic pu... more Flow rates of up to 50 µm s -1 have been successfully achieved in a circular AC electroosmotic pump. The flow rate was found to increase linearly with the applied voltage and to decrease linearly with the applied frequency. The pump is expected to be suitable for circular chromatography.

Research paper thumbnail of PDMS Electrospray Devices Fabricated by PDMS-Diamond-Coated SU-8 Masters

Micro Total Analysis Systems 2002, 2002

Research paper thumbnail of Multiplexed probing of cytochromes P450 using inductively coupled plasma mass spectrometry (ICP-MS)

Research paper thumbnail of Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye

Scientific Reports, 2015

Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established met... more Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating dyes (such as SYBR Green) into the PCR mixture allowed real-time reaction monitoring and extraction of quantitative information (qPCR). Probes with different excitation spectra enable multiplex qPCR of several DNA segments using multi-channel optical detection systems. Here we show multiplex qPCR using an economical EvaGreen-based system with single optical channel detection. Previously reported non quantitative multiplex realtime PCR techniques based on intercalating dyes were conducted once the PCR is completed by performing melting curve analysis (MCA). The technique presented in this paper is both qualitative and quantitative as it provides information about the presence of multiple DNA strands as well as the number of starting copies in the tested sample. Besides important internal control, multiplex qPCR also allows detecting concentrations of more than one DNA strand within the same sample. Detection of the avian influenza virus H7N9 by PCR is a well established method. Multiplex qPCR greatly enhances its specificity as it is capable of distinguishing both haemagglutinin (HA) and neuraminidase (NA) genes as well as their ratio.

Research paper thumbnail of Integration Of A Micro Liquid Chromatograph Onto A Silicon Chip

Proceedings of the International Solid-State Sensors and Actuators Conference - TRANSDUCERS '95, 1995

** SUMMARY A novel concept for small volume liquid chromatography is presented. Using micromachin... more ** SUMMARY A novel concept for small volume liquid chromatography is presented. Using micromachining techniques, a p€anar silicon structure containing the channel system has been fabricated. A split injector, a packed small-bore column, a frit and an optical detector cell were integrated onto a silicon chip, covered by a glass chip and another silicon optical fibre positioning unit. The overall

Research paper thumbnail of Why use a Delicate Biosensor for Monitoring? Alternative Routes by Miniaturizing and Speeding up the Classic Analytical Techniques

Biosensors '92 Proceedings, 1992

Research paper thumbnail of Trends in Microfluidic Devices for Analytical Chemistry

Research paper thumbnail of Characterization of electrophoretic sample injection and separation in a gel‐filled cyclic planar microstructure

Journal of Microcolumn Separations, 1996

ABSTRACT

Research paper thumbnail of Microsystems for Analysis in Flowing Solutions

Micro Total Analysis Systems, 1995

Research paper thumbnail of An Atmospheric Pressure Plasma on a Chip Applied as a Molecular Emission Detector in Gas Chromatography

Micro Total Analysis Systems 2000, 2000

Research paper thumbnail of Towards Evaporation-Driven HPLC on a Chip: An Alternative Transport Process for Micro Analysis Systems

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Optical Emission Detection of Liquid Analytes Using a Micro-Machined D.C. Glow-Discharge Device at Atmospheric Pressure

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Micro Total Analysis Systems

Research paper thumbnail of 3D-Flow Confinement for Efficient Sample Delivery — Application to Immunoassays on Planar Optical Waveguides

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device

Fresenius' journal of analytical chemistry, 2000

A micromachined capillary electrophoresis system has been fabricated on a glass device for the se... more A micromachined capillary electrophoresis system has been fabricated on a glass device for the separation and indirect fluorescence detection of phenols. Using this device two phenols viz., 2,4-dichlorophenol and pentachlorophenol, were separated within 12 s compared to under 19 min on a conventional capillary electrophoresis system using direct ultraviolet detection. The precision of the glass device ranged from 12.7%-16.7% compared to 0.42%-4.9% for the conventional system. Both systems showed good linearity in the concentration range of 0.8-6.38 mM for the glass device and 5-130 microM for the conventional system. The relationship between temperature and high voltage with baseline drift was also investigated. These results provide a foundation for the development of a miniaturised chemical analysis system for the on-line analysis of phenols in water.

Research paper thumbnail of Ultimate speed and sample volumes in electrophoresis

Biochemical Society transactions, 1997

Research paper thumbnail of A novel optical detector cell for use in miniaturized total chemical analysis systems

TRANSDUCERS '91: 1991 International Conference on Solid-State Sensors and Actuators. Digest of Technical Papers, 1991

ABSTRACT

Research paper thumbnail of Towards Organic Synthesis in Microfluidic Devices: Multicomponent Reactions for the Construction of Compound Libraries

Micro Total Analysis Systems 2000, 2000

Research paper thumbnail of Miniaturization of separation techniques using planar chip technology

Journal of High Resolution Chromatography, 1993

... Andreas Manz')*, Elisabeth Verpoorte'), Carlo S. Effenhauser'), Norbert Burggr... more ... Andreas Manz')*, Elisabeth Verpoorte'), Carlo S. Effenhauser'), Norbert Burggraf'), Daniel E. Raymond'), D. Jed Harrison') and H. Michael Widmer') 'kiba ... cell Figure 1 gives an example of one possible expenmental configuration, in whch hght from an external source is directed ...

Research paper thumbnail of A Miniaturized Glow Discharge Optical Emission Detector for Aqueous Analytes

Micro Total Analysis Systems 2002, 2002

ABSTRACT

Research paper thumbnail of A Circular AC Electroosmotic Micropump for Chromatographic Applications

Micro Total Analysis Systems 2002, 2002

Flow rates of up to 50 µm s -1 have been successfully achieved in a circular AC electroosmotic pu... more Flow rates of up to 50 µm s -1 have been successfully achieved in a circular AC electroosmotic pump. The flow rate was found to increase linearly with the applied voltage and to decrease linearly with the applied frequency. The pump is expected to be suitable for circular chromatography.

Research paper thumbnail of PDMS Electrospray Devices Fabricated by PDMS-Diamond-Coated SU-8 Masters

Micro Total Analysis Systems 2002, 2002

Research paper thumbnail of Multiplexed probing of cytochromes P450 using inductively coupled plasma mass spectrometry (ICP-MS)

Research paper thumbnail of Single Fluorescence Channel-based Multiplex Detection of Avian Influenza Virus by Quantitative PCR with Intercalating Dye

Scientific Reports, 2015

Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established met... more Since its invention in 1985 the polymerase chain reaction (PCR) has become a well-established method for amplification and detection of segments of double-stranded DNA. Incorporation of fluorogenic probe or DNA intercalating dyes (such as SYBR Green) into the PCR mixture allowed real-time reaction monitoring and extraction of quantitative information (qPCR). Probes with different excitation spectra enable multiplex qPCR of several DNA segments using multi-channel optical detection systems. Here we show multiplex qPCR using an economical EvaGreen-based system with single optical channel detection. Previously reported non quantitative multiplex realtime PCR techniques based on intercalating dyes were conducted once the PCR is completed by performing melting curve analysis (MCA). The technique presented in this paper is both qualitative and quantitative as it provides information about the presence of multiple DNA strands as well as the number of starting copies in the tested sample. Besides important internal control, multiplex qPCR also allows detecting concentrations of more than one DNA strand within the same sample. Detection of the avian influenza virus H7N9 by PCR is a well established method. Multiplex qPCR greatly enhances its specificity as it is capable of distinguishing both haemagglutinin (HA) and neuraminidase (NA) genes as well as their ratio.

Research paper thumbnail of Integration Of A Micro Liquid Chromatograph Onto A Silicon Chip

Proceedings of the International Solid-State Sensors and Actuators Conference - TRANSDUCERS '95, 1995

** SUMMARY A novel concept for small volume liquid chromatography is presented. Using micromachin... more ** SUMMARY A novel concept for small volume liquid chromatography is presented. Using micromachining techniques, a p€anar silicon structure containing the channel system has been fabricated. A split injector, a packed small-bore column, a frit and an optical detector cell were integrated onto a silicon chip, covered by a glass chip and another silicon optical fibre positioning unit. The overall

Research paper thumbnail of Why use a Delicate Biosensor for Monitoring? Alternative Routes by Miniaturizing and Speeding up the Classic Analytical Techniques

Biosensors '92 Proceedings, 1992

Research paper thumbnail of Trends in Microfluidic Devices for Analytical Chemistry

Research paper thumbnail of Characterization of electrophoretic sample injection and separation in a gel‐filled cyclic planar microstructure

Journal of Microcolumn Separations, 1996

ABSTRACT

Research paper thumbnail of Microsystems for Analysis in Flowing Solutions

Micro Total Analysis Systems, 1995

Research paper thumbnail of An Atmospheric Pressure Plasma on a Chip Applied as a Molecular Emission Detector in Gas Chromatography

Micro Total Analysis Systems 2000, 2000

Research paper thumbnail of Towards Evaporation-Driven HPLC on a Chip: An Alternative Transport Process for Micro Analysis Systems

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Optical Emission Detection of Liquid Analytes Using a Micro-Machined D.C. Glow-Discharge Device at Atmospheric Pressure

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Micro Total Analysis Systems

Research paper thumbnail of 3D-Flow Confinement for Efficient Sample Delivery — Application to Immunoassays on Planar Optical Waveguides

Micro Total Analysis Systems 2001, 2001

Research paper thumbnail of Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device

Fresenius' journal of analytical chemistry, 2000

A micromachined capillary electrophoresis system has been fabricated on a glass device for the se... more A micromachined capillary electrophoresis system has been fabricated on a glass device for the separation and indirect fluorescence detection of phenols. Using this device two phenols viz., 2,4-dichlorophenol and pentachlorophenol, were separated within 12 s compared to under 19 min on a conventional capillary electrophoresis system using direct ultraviolet detection. The precision of the glass device ranged from 12.7%-16.7% compared to 0.42%-4.9% for the conventional system. Both systems showed good linearity in the concentration range of 0.8-6.38 mM for the glass device and 5-130 microM for the conventional system. The relationship between temperature and high voltage with baseline drift was also investigated. These results provide a foundation for the development of a miniaturised chemical analysis system for the on-line analysis of phenols in water.

Research paper thumbnail of Ultimate speed and sample volumes in electrophoresis

Biochemical Society transactions, 1997

Research paper thumbnail of A novel optical detector cell for use in miniaturized total chemical analysis systems

TRANSDUCERS '91: 1991 International Conference on Solid-State Sensors and Actuators. Digest of Technical Papers, 1991

ABSTRACT

Research paper thumbnail of Towards Organic Synthesis in Microfluidic Devices: Multicomponent Reactions for the Construction of Compound Libraries

Micro Total Analysis Systems 2000, 2000

Research paper thumbnail of Miniaturization of separation techniques using planar chip technology

Journal of High Resolution Chromatography, 1993

... Andreas Manz')*, Elisabeth Verpoorte'), Carlo S. Effenhauser'), Norbert Burggr... more ... Andreas Manz')*, Elisabeth Verpoorte'), Carlo S. Effenhauser'), Norbert Burggraf'), Daniel E. Raymond'), D. Jed Harrison') and H. Michael Widmer') 'kiba ... cell Figure 1 gives an example of one possible expenmental configuration, in whch hght from an external source is directed ...

Research paper thumbnail of A Miniaturized Glow Discharge Optical Emission Detector for Aqueous Analytes

Micro Total Analysis Systems 2002, 2002

ABSTRACT