María Olivera - Academia.edu (original) (raw)
Papers by María Olivera
The development of new treatments capable of controlling infections and pain related to burns con... more The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells.In this study, antibiotic-anesthetic smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as film and hydrogel. The complexation of ciprofloxacin with natural polyelectrolytes efficiently contributed to increasing biocompatibility in a primary culture of isolated fibroblasts. In addition, the relative levels of the proteins integrin β1 and p-FAK involved in cell migration were increased with no modifications in cell mobility. Their evaluation in a deep second-degree burn model revealed fast reepithelization, with appendage conservation and complete dermis organization. Encouragingly, we found that both the film and the hydrogel showed a significantly superior performance compared to the reference treatment of silver sul...
Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Arge... more Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina.
Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are ... more Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are preventing and reducing the complications which cause more than 70% of deaths. Health programs with drug supply at public health facilities in Alta Gracia City were analized: Hospital Arturo Umberto Illia (HAUI), depending on the Health Ministry of the Province of Cordoba, and 12 dispensaries, depending on the Direccion de Salud Publica (DSP) of the Municipality of Alta Gracia. A total of 540 patients were identified in the merged data base and 49 were found in both settings. Drug consumptions, expressed in DDD per 100 patient under program by day, were: 25.2 in HAUI and 24.6 in DSP for Glibenclamide; 17.6 in HAUI and 13.1 in DSP for Metformin; 59.0 in HAUI for Insulin. The information obtained from the Pharmacy Services let the development of a diabetic patient data base and a consumption drug utilization study.
Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el s... more Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el subsector publico de salud de la ciudad de Alta Gracia. Analizar el uso de la cantidad de dispensaciones mensuales como indicador de adherencia al tratamiento, comparando resultados entre establecimientos sanitarios. Valorar la coordinacion de efectores publicos en la atencion de pacientes. Materiales y metodos: Diseno: estudio observacional, descriptivo-analitico. Lugar: Hospital Arturo Umberto Illia (HAUI) y Direccion de Salud Publica (DSP). Alta Gracia (Provincia de Cordoba, Argentina). Poblacion: Pacientes diabeticos pertenecientes a programas de salud de efectores publicos. Mediciones principales: Variables demograficas y epidemiologicas, cantidad de dispensaciones mensuales, adherencia global porcentual y coordinacion de efectores. Resultados: Del total de pacientes diabeticos (n=540): 52% se atendian en el HAUI, 39% en la DSP y 9% en ambos centros; 55% eran mujeres y la edad promed...
Drug delivery and translational research, Feb 1, 2018
This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were de... more This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were developed based on a ciprofloxacin-based swellable drug polyelectrolyte matrix (SDPM-CIP). The matrix contains a molecular dispersion of ciprofloxacin ionically bonded to the acidic groups of carbomer, forming the polyelectrolyte-drug complex CB-CIP. This formulation showed that the release profile of the ciprofloxacin bilayer tablets currently commercialised can be achieved with a simpler strategy. Thus, since ciprofloxacin urine concentrations are associated with the clinical cure of urinary tract infections, the goal of this work was to compare the urinary excretion of SDPM-CIP tablets with those of the CIPRO XR® bilayer tablets. A batch of SDPM-CIP tablets was manufactured by the wet granulation method and the CB-CIP ionic complex was obtained in situ. Fasted healthy volunteers received a single oral dose of 500 mg ciprofloxacin of either formulation in a randomised crossover study. Ur...
Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina), Dec 26, 2018
Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment ... more Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extrac...
Latin American Journal of Pharmacy, 2008
Journal of Pharmacy and Pharmacology, 2015
Objectives Recent evidence associates omega-3 fatty acids (O3) with pain reduction. The aim of th... more Objectives Recent evidence associates omega-3 fatty acids (O3) with pain reduction. The aim of this work was to evaluate the antinociceptive effect of O3, either alone or in combination with morphine after acute and chronic administration in rats. As well, a new pharmaceutical mixture that allows the concomitant administration of O3 and morphine as an oral solution was developed. Methods Animals were fed on a control or an experimental diet supplemented with O3. They were subjected to the hot-plate test to assess analgesic effect and tolerance to the analgesic effect of morphine. The open-field test was carried out to determine if the differences in the response latency can be related to non-specific sedative effects. Key findings O3 dietary supplementation increased the response latency compared with the control group. Acute treatment with morphine in these groups resulted in an additive antinociceptive effect not related to locomotor activity. Chronic coadministration of morphine ...
International journal of pharmaceutics, Jan 17, 2009
The hydrochlorides of the 1:3 aluminum:norfloxacin and aluminum:ciprofloxacin complexes were char... more The hydrochlorides of the 1:3 aluminum:norfloxacin and aluminum:ciprofloxacin complexes were characterized according to the Biopharmaceutics Classification System (BCS) premises in comparison with their parent compounds. The pH-solubility profiles of the complexes were experimentally determined at 25 and 37 degrees C in the range of pH 1-8 and compared to that of uncomplexed norfloxacin and ciprofloxacin. Both complexes are clearly more soluble than the antibiotics themselves, even at the lowest solubility pHs. The increase in solubility was ascribed to the species controlling solubility, which were analyzed in the solid phases at equilibrium at selected pHs. Additionally, permeability was set as low, based on data reported in the scientific literature regarding oral bioavailability, intestinal and cell cultures permeabilities and also considering the influence of stoichiometric amounts of aluminum. The complexes fulfill the BCS criterion to be classified as class 3 compounds (high ...
![Research paper thumbnail of [Importance of the information systems in public health programs: diabetic patients databases]](https://attachments.academia-assets.com/84565023/thumbnails/1.jpg)
](Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2012
To describe the characteristics of diabetic patients' population attended in the public healt... more To describe the characteristics of diabetic patients' population attended in the public health sector of Alta Gracia city. To analyze the use of amount of monthly dispensations as indicator of adherence to treatment, by comparing the results between settings. To assess the coordination of public facilities for patients attention. observational descriptive-analytical study. Hospital Arturo Umberto Illia (HAUI) and Dirección de Salud Pública (DSP). Alta Gracia (province of Córdoba, Argentina). diabetic patients belonging to health programs at public facilities. demographic and epidemiological variables, amount of monthly dispensations, percentage of global adherence and coordination of settings. From diabetic patients' total (n=540): 52% were attended at HAUI, 39% at DSP, and 9% at both settings; 55% were female, and the average age was about 56 years old; 81% were type 2 Diabetes Mellitus (DM), 1% type 2 insulin-requiring DM, and 17% type 1 DM; the general mean of amount of m...
European Journal of Pharmaceutical Sciences, 2003
A set of potential Class III antiarrhythmic agents of structure p-HOOC-R-CO-NH-C(6)H(4)-CO-X-C(2)... more A set of potential Class III antiarrhythmic agents of structure p-HOOC-R-CO-NH-C(6)H(4)-CO-X-C(2)H(5)-N(C(2)H(5))(2) were isolated as crystalline solids of the amide and ester derivatives, I: succinylprocainamide (X=-NH-, R=-C(2)H(4)-); II: succinylprocaine (X=-O-, R=-C(2)H(4)-); III: maleylprocainamide (X=-NH-, R=-C(2)H(2)-) and IV: maleylprocaine (X=-O-, R=-C(2)H(2)-). Although compounds I-IV exhibit similar solution properties (i.e. acid-base speciation, with zwitterionic (+-) to neutral (00) form ratios higher than 10(4)), aqueous solubility of -NH- derivatives is significantly higher than that of -O- derivatives and also, solvent effects on solubility (i.e. the change of water by ethanol) is clearly different in both series. Solution and solid-state properties of I-IV were characterized to account for the observed differences. Results indicate that procainamide derivatives I and III crystallizes as (+-)(s) but procaine derivatives II and IV as (00)(s). Besides, I is anhydrous but II-IV are hydrates. Aqueous solubility and solvent effect on solubility are controlled by the intrinsic solubility of the species (+-) in I and III and (00) in II and IV. The rise of hydrophilicity of species (00) due to the structural change from -O- to -NH- would determine the change in the structure of the precipitating crystals from (00)(s) to (+-)(s). Solid structure (zwitterionic or neutral), as well as composition (anhydrous or hydrated) may be recognized as the main factors in determining the rank of aqueous solubility of the set: (+-)>(+-.H(2)O)>(00.H(2)O).
European Journal of Pharmaceutical Sciences, 2008
acta farmacéutica …, 2004
Farmacia hospitalaria : órgano oficial de expresión científica de la Sociedad Española de Farmacia Hospitalaria
To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under differen... more To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1 °C) and the rest of the samples were stored in the fridge (5 ± 2 °C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. At least 98% of the initial chloral hydrate concentration remained throughou...
SUMMARY. To assess the inpatients consumption of omeprazole and ranitidine in a provincial public... more SUMMARY. To assess the inpatients consumption of omeprazole and ranitidine in a provincial public hospital in Córdoba, Argentina, a transversal observational study was carried out showing the consumption of these drugs from June to November in 2004 and 2005, and checking out diagnosis of inpatients using such drugs during August, September and October 2006. Total consumption of antiulcer drugs was 3,261.33 defined daily dosed (DDD) in 2004 and 3,154.17 in 2005, corresponding to ranitidine mostly. DDD/100 bed-days per clinical services were calculated at Intensive Care Unit, Internal Medicine, and General Surgery. Non clinical protocols had been established to promote and improve rational use of drugs. Most of inpatients had no explicit diagnosis to justify the prescription of antiulcer drugs. An antiulcer drug utilization study was carried out collecting useful information from pharmacy service by hand. RESUMEN. “Uso de Ranitidina y Omeprazol en un Hospital Público de Argentina”. Co...
Pharmacy Education, 2018
The need to know the current state of professional practice in Argentina in different areas, and ... more The need to know the current state of professional practice in Argentina in different areas, and the absence of systematic information about it, led to the organisation of a workshop with pharmacists from several different practice settings. The workshop provided a space for reflection and dialogue for the participants committed to improving pharmacy education and professional practice in Argentina. The objective of this report was to document the main points from this workshop; which allowed the participants to reinforce their identity as pharmacists, review health policies in Argentina and identify the strengths and weaknesses of pharmacy education and professional practice in order to work on the gaps observed. A list of future actions to be implemented in the work setting was drawn up and the representatives who would allow the implementation of necessary action plans and who expressed their commitment to act upon them were identified. This experience highlighted the need to str...
Drug Delivery and Translational Research
The in vivo release segregation of rifampicin (RIF) and isoniazid (INH) has been proposed as a st... more The in vivo release segregation of rifampicin (RIF) and isoniazid (INH) has been proposed as a strategy to avoid RIF acid degradation, which is known as one of the main factors for reduced RIF bioavailability and can result in drug-resistant tuberculosis. So far, this strategy has been scarcely explored. The aims of this study were to investigate the stability and bioavailability of RIF after combination of a very fast release matrix of RIF with a sustained delivery system of INH. A series of INH-alginic acid complexes (AA-INH) was obtained and characterized. Independent and sequential release profile of AA-INH at biorrelevant media of pH 1.20 and 6.80 was explored. In addition, AA-INH was combined with a RIF-carboxymethylcellulose very fast release complex (CMC-RIF) obtained previously and subjected to acid dissolution assays to evaluate RIF acid stability and determine RIF and INH dissolution efficiencies. Finally, a pharmacokinetic study in dogs was carried out. The AA-INH was easily obtained in solid-state. Their characterization revealed its ionic nature, with a loading capacity of around 30%. The dissolution efficiencies (15 min) confirmed release segregation in acid media with 7.8 and 65.6% for AA-INH and CMC-RIF, respectively. INH release rate from the AA-INH system was slow in acid media and increased in simulated intestinal media. The complete release of INH was achieved after 2 h in simulated intestinal media in the sequential release experiments. The acid degradation of RIF was significantly reduced (36.7%) when both systems were combined and oral administration to dogs revealed a 42% increase in RIF bioavailability. In conclusion, CMC-RIF and AA-INH may be useful for the formulation of a site-specific solid dosage form to overcome some of the main obstacles in tuberculosis treatment. Graphical abstract.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 3, 2018
Benznidazole (BZ), first-line drug for Chagas treatment, is available as immediate-release tablet... more Benznidazole (BZ), first-line drug for Chagas treatment, is available as immediate-release tablets. High frequency of administration, long-term therapy, and side effects of BZ conspire against treatment adherence, and negatively impact in therapeutic success. We have developed BZ-loaded interpolyelectrolyte complexes (IPECs) composed of polymethacrylates (EE-EL-BZ) or polysaccharides (Ch-AA-BZ) for controlled BZ release. This work aimed to evaluate their preclinical pharmacokinetics compared to Abarax® (reference treatment) and to correlate them with the in vitro BZ release. A randomization schedule with a 3 × 2 cross-over design was used. Each healthy dog received a single oral dose of 100 mg of BZ from EE-EL-BZ, Ch-AA-BZ or Abarax®. BZ quantification was performed in plasma by a validated HPLC-UV method. Moreover, in silico simulations using the pharmacokinetic software PK Solutions 2.0™ were calculated for the multiple-dose administration at two dose regimens: 100 mg of BZ admini...
The development of new treatments capable of controlling infections and pain related to burns con... more The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells.In this study, antibiotic-anesthetic smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as film and hydrogel. The complexation of ciprofloxacin with natural polyelectrolytes efficiently contributed to increasing biocompatibility in a primary culture of isolated fibroblasts. In addition, the relative levels of the proteins integrin β1 and p-FAK involved in cell migration were increased with no modifications in cell mobility. Their evaluation in a deep second-degree burn model revealed fast reepithelization, with appendage conservation and complete dermis organization. Encouragingly, we found that both the film and the hydrogel showed a significantly superior performance compared to the reference treatment of silver sul...
Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Arge... more Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina.
Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are ... more Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are preventing and reducing the complications which cause more than 70% of deaths. Health programs with drug supply at public health facilities in Alta Gracia City were analized: Hospital Arturo Umberto Illia (HAUI), depending on the Health Ministry of the Province of Cordoba, and 12 dispensaries, depending on the Direccion de Salud Publica (DSP) of the Municipality of Alta Gracia. A total of 540 patients were identified in the merged data base and 49 were found in both settings. Drug consumptions, expressed in DDD per 100 patient under program by day, were: 25.2 in HAUI and 24.6 in DSP for Glibenclamide; 17.6 in HAUI and 13.1 in DSP for Metformin; 59.0 in HAUI for Insulin. The information obtained from the Pharmacy Services let the development of a diabetic patient data base and a consumption drug utilization study.
Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el s... more Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el subsector publico de salud de la ciudad de Alta Gracia. Analizar el uso de la cantidad de dispensaciones mensuales como indicador de adherencia al tratamiento, comparando resultados entre establecimientos sanitarios. Valorar la coordinacion de efectores publicos en la atencion de pacientes. Materiales y metodos: Diseno: estudio observacional, descriptivo-analitico. Lugar: Hospital Arturo Umberto Illia (HAUI) y Direccion de Salud Publica (DSP). Alta Gracia (Provincia de Cordoba, Argentina). Poblacion: Pacientes diabeticos pertenecientes a programas de salud de efectores publicos. Mediciones principales: Variables demograficas y epidemiologicas, cantidad de dispensaciones mensuales, adherencia global porcentual y coordinacion de efectores. Resultados: Del total de pacientes diabeticos (n=540): 52% se atendian en el HAUI, 39% en la DSP y 9% en ambos centros; 55% eran mujeres y la edad promed...
Drug delivery and translational research, Feb 1, 2018
This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were de... more This paper builds on a previous paper in which new ciprofloxacin extended-release tablets were developed based on a ciprofloxacin-based swellable drug polyelectrolyte matrix (SDPM-CIP). The matrix contains a molecular dispersion of ciprofloxacin ionically bonded to the acidic groups of carbomer, forming the polyelectrolyte-drug complex CB-CIP. This formulation showed that the release profile of the ciprofloxacin bilayer tablets currently commercialised can be achieved with a simpler strategy. Thus, since ciprofloxacin urine concentrations are associated with the clinical cure of urinary tract infections, the goal of this work was to compare the urinary excretion of SDPM-CIP tablets with those of the CIPRO XR® bilayer tablets. A batch of SDPM-CIP tablets was manufactured by the wet granulation method and the CB-CIP ionic complex was obtained in situ. Fasted healthy volunteers received a single oral dose of 500 mg ciprofloxacin of either formulation in a randomised crossover study. Ur...
Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina), Dec 26, 2018
Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment ... more Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extrac...
Latin American Journal of Pharmacy, 2008
Journal of Pharmacy and Pharmacology, 2015
Objectives Recent evidence associates omega-3 fatty acids (O3) with pain reduction. The aim of th... more Objectives Recent evidence associates omega-3 fatty acids (O3) with pain reduction. The aim of this work was to evaluate the antinociceptive effect of O3, either alone or in combination with morphine after acute and chronic administration in rats. As well, a new pharmaceutical mixture that allows the concomitant administration of O3 and morphine as an oral solution was developed. Methods Animals were fed on a control or an experimental diet supplemented with O3. They were subjected to the hot-plate test to assess analgesic effect and tolerance to the analgesic effect of morphine. The open-field test was carried out to determine if the differences in the response latency can be related to non-specific sedative effects. Key findings O3 dietary supplementation increased the response latency compared with the control group. Acute treatment with morphine in these groups resulted in an additive antinociceptive effect not related to locomotor activity. Chronic coadministration of morphine ...
International journal of pharmaceutics, Jan 17, 2009
The hydrochlorides of the 1:3 aluminum:norfloxacin and aluminum:ciprofloxacin complexes were char... more The hydrochlorides of the 1:3 aluminum:norfloxacin and aluminum:ciprofloxacin complexes were characterized according to the Biopharmaceutics Classification System (BCS) premises in comparison with their parent compounds. The pH-solubility profiles of the complexes were experimentally determined at 25 and 37 degrees C in the range of pH 1-8 and compared to that of uncomplexed norfloxacin and ciprofloxacin. Both complexes are clearly more soluble than the antibiotics themselves, even at the lowest solubility pHs. The increase in solubility was ascribed to the species controlling solubility, which were analyzed in the solid phases at equilibrium at selected pHs. Additionally, permeability was set as low, based on data reported in the scientific literature regarding oral bioavailability, intestinal and cell cultures permeabilities and also considering the influence of stoichiometric amounts of aluminum. The complexes fulfill the BCS criterion to be classified as class 3 compounds (high ...
Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2012
To describe the characteristics of diabetic patients' population attended in the public healt... more To describe the characteristics of diabetic patients' population attended in the public health sector of Alta Gracia city. To analyze the use of amount of monthly dispensations as indicator of adherence to treatment, by comparing the results between settings. To assess the coordination of public facilities for patients attention. observational descriptive-analytical study. Hospital Arturo Umberto Illia (HAUI) and Dirección de Salud Pública (DSP). Alta Gracia (province of Córdoba, Argentina). diabetic patients belonging to health programs at public facilities. demographic and epidemiological variables, amount of monthly dispensations, percentage of global adherence and coordination of settings. From diabetic patients' total (n=540): 52% were attended at HAUI, 39% at DSP, and 9% at both settings; 55% were female, and the average age was about 56 years old; 81% were type 2 Diabetes Mellitus (DM), 1% type 2 insulin-requiring DM, and 17% type 1 DM; the general mean of amount of m...
European Journal of Pharmaceutical Sciences, 2003
A set of potential Class III antiarrhythmic agents of structure p-HOOC-R-CO-NH-C(6)H(4)-CO-X-C(2)... more A set of potential Class III antiarrhythmic agents of structure p-HOOC-R-CO-NH-C(6)H(4)-CO-X-C(2)H(5)-N(C(2)H(5))(2) were isolated as crystalline solids of the amide and ester derivatives, I: succinylprocainamide (X=-NH-, R=-C(2)H(4)-); II: succinylprocaine (X=-O-, R=-C(2)H(4)-); III: maleylprocainamide (X=-NH-, R=-C(2)H(2)-) and IV: maleylprocaine (X=-O-, R=-C(2)H(2)-). Although compounds I-IV exhibit similar solution properties (i.e. acid-base speciation, with zwitterionic (+-) to neutral (00) form ratios higher than 10(4)), aqueous solubility of -NH- derivatives is significantly higher than that of -O- derivatives and also, solvent effects on solubility (i.e. the change of water by ethanol) is clearly different in both series. Solution and solid-state properties of I-IV were characterized to account for the observed differences. Results indicate that procainamide derivatives I and III crystallizes as (+-)(s) but procaine derivatives II and IV as (00)(s). Besides, I is anhydrous but II-IV are hydrates. Aqueous solubility and solvent effect on solubility are controlled by the intrinsic solubility of the species (+-) in I and III and (00) in II and IV. The rise of hydrophilicity of species (00) due to the structural change from -O- to -NH- would determine the change in the structure of the precipitating crystals from (00)(s) to (+-)(s). Solid structure (zwitterionic or neutral), as well as composition (anhydrous or hydrated) may be recognized as the main factors in determining the rank of aqueous solubility of the set: (+-)>(+-.H(2)O)>(00.H(2)O).
European Journal of Pharmaceutical Sciences, 2008
acta farmacéutica …, 2004
Farmacia hospitalaria : órgano oficial de expresión científica de la Sociedad Española de Farmacia Hospitalaria
To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under differen... more To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1 °C) and the rest of the samples were stored in the fridge (5 ± 2 °C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. At least 98% of the initial chloral hydrate concentration remained throughou...
SUMMARY. To assess the inpatients consumption of omeprazole and ranitidine in a provincial public... more SUMMARY. To assess the inpatients consumption of omeprazole and ranitidine in a provincial public hospital in Córdoba, Argentina, a transversal observational study was carried out showing the consumption of these drugs from June to November in 2004 and 2005, and checking out diagnosis of inpatients using such drugs during August, September and October 2006. Total consumption of antiulcer drugs was 3,261.33 defined daily dosed (DDD) in 2004 and 3,154.17 in 2005, corresponding to ranitidine mostly. DDD/100 bed-days per clinical services were calculated at Intensive Care Unit, Internal Medicine, and General Surgery. Non clinical protocols had been established to promote and improve rational use of drugs. Most of inpatients had no explicit diagnosis to justify the prescription of antiulcer drugs. An antiulcer drug utilization study was carried out collecting useful information from pharmacy service by hand. RESUMEN. “Uso de Ranitidina y Omeprazol en un Hospital Público de Argentina”. Co...
Pharmacy Education, 2018
The need to know the current state of professional practice in Argentina in different areas, and ... more The need to know the current state of professional practice in Argentina in different areas, and the absence of systematic information about it, led to the organisation of a workshop with pharmacists from several different practice settings. The workshop provided a space for reflection and dialogue for the participants committed to improving pharmacy education and professional practice in Argentina. The objective of this report was to document the main points from this workshop; which allowed the participants to reinforce their identity as pharmacists, review health policies in Argentina and identify the strengths and weaknesses of pharmacy education and professional practice in order to work on the gaps observed. A list of future actions to be implemented in the work setting was drawn up and the representatives who would allow the implementation of necessary action plans and who expressed their commitment to act upon them were identified. This experience highlighted the need to str...
Drug Delivery and Translational Research
The in vivo release segregation of rifampicin (RIF) and isoniazid (INH) has been proposed as a st... more The in vivo release segregation of rifampicin (RIF) and isoniazid (INH) has been proposed as a strategy to avoid RIF acid degradation, which is known as one of the main factors for reduced RIF bioavailability and can result in drug-resistant tuberculosis. So far, this strategy has been scarcely explored. The aims of this study were to investigate the stability and bioavailability of RIF after combination of a very fast release matrix of RIF with a sustained delivery system of INH. A series of INH-alginic acid complexes (AA-INH) was obtained and characterized. Independent and sequential release profile of AA-INH at biorrelevant media of pH 1.20 and 6.80 was explored. In addition, AA-INH was combined with a RIF-carboxymethylcellulose very fast release complex (CMC-RIF) obtained previously and subjected to acid dissolution assays to evaluate RIF acid stability and determine RIF and INH dissolution efficiencies. Finally, a pharmacokinetic study in dogs was carried out. The AA-INH was easily obtained in solid-state. Their characterization revealed its ionic nature, with a loading capacity of around 30%. The dissolution efficiencies (15 min) confirmed release segregation in acid media with 7.8 and 65.6% for AA-INH and CMC-RIF, respectively. INH release rate from the AA-INH system was slow in acid media and increased in simulated intestinal media. The complete release of INH was achieved after 2 h in simulated intestinal media in the sequential release experiments. The acid degradation of RIF was significantly reduced (36.7%) when both systems were combined and oral administration to dogs revealed a 42% increase in RIF bioavailability. In conclusion, CMC-RIF and AA-INH may be useful for the formulation of a site-specific solid dosage form to overcome some of the main obstacles in tuberculosis treatment. Graphical abstract.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 3, 2018
Benznidazole (BZ), first-line drug for Chagas treatment, is available as immediate-release tablet... more Benznidazole (BZ), first-line drug for Chagas treatment, is available as immediate-release tablets. High frequency of administration, long-term therapy, and side effects of BZ conspire against treatment adherence, and negatively impact in therapeutic success. We have developed BZ-loaded interpolyelectrolyte complexes (IPECs) composed of polymethacrylates (EE-EL-BZ) or polysaccharides (Ch-AA-BZ) for controlled BZ release. This work aimed to evaluate their preclinical pharmacokinetics compared to Abarax® (reference treatment) and to correlate them with the in vitro BZ release. A randomization schedule with a 3 × 2 cross-over design was used. Each healthy dog received a single oral dose of 100 mg of BZ from EE-EL-BZ, Ch-AA-BZ or Abarax®. BZ quantification was performed in plasma by a validated HPLC-UV method. Moreover, in silico simulations using the pharmacokinetic software PK Solutions 2.0™ were calculated for the multiple-dose administration at two dose regimens: 100 mg of BZ admini...