Marcella Bernal - Academia.edu (original) (raw)

Papers by Marcella Bernal

Research paper thumbnail of Adenocarcinoma Pancreático Diferenciado De Tecido Ectópico Em Felino –Relato De Caso

INVESTIGAÇÃO, Sep 12, 2018

Research paper thumbnail of Leptospirosis, bovine viral diarrhea and infectious bovine rhinotracheitis: prevalence in Colombian cattle and buffaloes

Acta Scientiarum. Animal Sciences

One of the limiting factors of productive efficiency in cattle and buffalo herds is related to th... more One of the limiting factors of productive efficiency in cattle and buffalo herds is related to the high prevalence of infectious diseases which affect reproduction. The aim of this study was to determine the prevalence of anti-Leptospira antibodies for bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) in bovine and buffalo herds in Colombia. Blood serum samples were collected from 1100 buffaloes and 1000 cattle. The ELISA technique was used to detect antibodies against BVDV and BoHV-1, and the microscopic agglutination technique to detect anti-Leptospira antibodies. The prevalence of anti-Leptospira antibodies and of BVDV and BoHV-1 in bovine samples was observed in 16, 39.7, and 65% of animals, respectively, while the positivity in samples for the same antibodies in buffalos was detected in 18.7, 27.5 and 51.5%, respectively. Exposure of cattle and buffaloes to BoHV-1 was positively associated with age, higher prevalence rates were observed in older ages. Se...

Research paper thumbnail of Estudo de Arbovírus em Philander opossum, Didelphis marsupialis e Nectomys rattus capturados em fragmentos florestais no município de Belém, Pará

Research paper thumbnail of Innate immunity gene expression profiles in conjunctival membrane biopsies from Amazonian buffalo

Acta Amazonica, 2022

Domestic buffalo production plays an economically important role in the Brazilian Amazon, but the... more Domestic buffalo production plays an economically important role in the Brazilian Amazon, but they are susceptible to many diseases favored by the tropical climate and annually flooded habitats, including ocular diseases. In this context, it is important to select genotypes that maximize innate ocular immunity in Amazonian herds. We aimed to characterise, for the first time, gene expression profiles of the innate immune system in the conjunctival membrane of buffalo. Ocular conjunctival tissue samples were collected from 60 clinically healthy slaughtered animals in the northern Brazilian state of Amapá. The samples were histologically processed for classification into three groups according to the quantitative degree of lymphoid tissue associated with the conjunctiva (discrete, G1; slight, G2; and moderate, G3 presence of lymphoid tissue). RT-PCR was used to quantify gene expression of inflammatory cytokine (IL6, IL10, TNFA, IFNG), Toll-like receptor 4 (TLR4), and Defensin beta 110 ...

Research paper thumbnail of Experiencias de la aplicación de fitorreguladores para mejorar la calidad de la uva

Research paper thumbnail of La mejora de la calidad en los servicios de la universidad: experiencias desarrolladas en la secretaría de un departameto universitario

Materiales Para La Calidad Actas De Las Ii Jornadas Andaluzas De Calidad En La Ensenanza Universitaria Desarrollo De Planes De Calidad Para La Universidad Vol 1 2000 Isbn 84 86849 21 7 Pags 121 130, 2000

Research paper thumbnail of Diagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimerase

Research paper thumbnail of Reações teciduais induzidas pela abraçadeira autoestática de náilon em ligaduras de pedículos ovarianos

Biotemas

Objetivou-se avaliar as reações teciduais induzidas pela abraçadeira autoestática de náilon na oc... more Objetivou-se avaliar as reações teciduais induzidas pela abraçadeira autoestática de náilon na oclusão dos pedículos ovarianos, por meio de avaliação macroscópica e histológica. Utilizaram-se 45 fêmeas caninas, sem raça definida, clinicamente sadias, com idade média de 31,11 ± 14,26 meses e peso médio de 11,26 ± 4,7 kg. Todas foram submetidas à ovariosalpingohisterectomia pela técnica minimamente invasiva. Os animais foram divididos aleatoriamente em três grupos com 15 animais cada, que foram avaliados no pós-cirúrgico imediato e aos 30 (G1), 60 (G2) e 90 dias (G3) de pós-operatório. Ao exame histológico dos pedículos contendo as abraçadeiras foi observada, no 30º dia de pós-operatório, reação inflamatória crônica com macrófagos, células gigantes e fibroplasia. No 60º dia, tecido conjuntivo totalmente organizado e em modelação, e presença de linfócitos e polimorfonucleares. No 90º dia de pós-operatório, tecido conjuntivo maduro totalmente organizado com áreas em modelação, macrófago...

Research paper thumbnail of Achados Anatomopatológicos De Toxoplasma Sp. Em Lagothrix Lagotricha Da Região Amazônica

Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmissio... more Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmission, and a biological cycle of the heteroxenic type, capable of infecting a wide variety of homeothermic animals, including non-human primates and human. The evolution of the disease occurs in an acute and chronic form, depending on the parasite-host relationship, the physiological condition of the infected and virulence, which can lead to death. In this report, the autopsy of three specimens of Lagothrix lagotricha was performed. In the necroscopic examination of the specimens, pulmonary edema and an increase in the size of the mesenteric and submandibular lymph nodes, liver and spleen were observed. Tissue samples were collected and fixed in 10% buffered formaldehyde, processed and analyzed. Histopathology identified structures of T. gondii in the forms of cystzoites in lymph node tissues, cardiac musculature, lungs, spleen and liver, and tachyzoites in liver tissue. It is concluded that...

Research paper thumbnail of Serological markers for hepatitis a among captive and free-living wild mammals in the State of Pará, Brazil

Semina-ciencias Agrarias, 2021

The hepatitis A virus (HAV, Hepatovirus A) in humans has a relevant impact on public health, espe... more The hepatitis A virus (HAV, Hepatovirus A) in humans has a relevant impact on public health, especially in geographic regions with poor basic sanitation, such as the Brazilian Amazon. Isolates related to hepatoviruses have been described in non human primates, chiropterans, marsupials, rodents, marmots, shrews, and terrestrial hedgehogs. However, the circulation of these viruses in the Amazonian mammal fauna remains unexplored. This study aimed to evaluate the prevalence of antibodies against this hepatovirus in captive and free-living wild mammals belonging to the orders Didelphimorphia, Primates, Carnivora, and Artiodactyla. Serum samples from 71 animals, from three municipalities in the State of Pará (Belém, Santarém, and CapitãoPoço) were tested for total anti-HAV and anti-HAV IgM through enzyme-linked immunosorbent assay (ELISA). Total anti-HAV antibodies were detected in 29.5% (21/71) of non-human primates, 8.4% (6/71) of carnivores, 1 Student of the Doctoral Course of the Pós...

Research paper thumbnail of Cryptosporidium spp. oocysts and Giardia spp. cysts in coatis (Nasua nasua L. 1766) from Pará, Brazil

Acta Veterinaria Brasilica, Sep 30, 2017

Article history Zoonotic enteroparasitosis represent an important public health problem, and spec... more Article history Zoonotic enteroparasitosis represent an important public health problem, and species of protozoa such as Cryptosporidium spp. and Giardia spp. can reach high frequencies in regions where basic sanitation conditions are poor, which promotes outbreaks of diarrhea in humans and domestic and wild animals. Wild mammals such as the South American coati (Nasua nasua) feed on insects present in the soil, fruits, and small vertebrates, and are susceptible to contamination by enteroparasites present in the environmental niche. The aim of the present study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in N. nasua from a region in the Brazilian Amazon. Fecal samples of 27 coatis two from free-living and 25 from captivity were collected in three different municipalities in the state of Pará, Brazil. The search for Giardia spp. and Cryptosporidium spp. in the collected samples were performed using the direct and Kinyoun methods, respectively, and a commercial immunological test (RIDA®QUICK Cryptosporidium/Giardia Combi. ®R-Biopharm) was used to detect antigens from both parasites. Cryptosporidium spp. oocysts were found in 11.1% (3/27) of the samples; one from a free-living animal and two from captive animals. Giardia spp. cysts were found in 11.1% (3/27) of the samples, all from captive animals. This is the first report of infection by these protozoans in this coati's species in the North region of Brazil; the South American coati may be participating as maintainers and disseminators of infectious agents to the environment and other hosts.

Research paper thumbnail of Uterine adenomyosis in bitches and queens: occurrence and histopathological aspects

Acta Veterinaria Brasilica

Article history The goal of this study was to report the occurrence and histopathological aspects... more Article history The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.

Research paper thumbnail of Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil

Semina: Ciências Agrárias

Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the ... more Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was ...

Research paper thumbnail of Infiltrative Rectal Adenocarcinoma in a Dog

Acta Scientiae Veterinariae

Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological... more Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological type found. This neoplasm presents slow growth and high capacity of causing metastasis. Histologically speaking, neoplasm cells can present solid, tubular, papillary arrangement and note amorphous extra-cellular material. Clinically observed tenesmus, diarrhea, dyskinesia, hematochezia, mane, protrusion of the anus, weight loss, anorexia. The occurrence and clinicopathological aspects of tumors in dogs’ gastrointestinal tract, the rectal segment, remains poorly understood. Accordingly, the aim of the present study is to report a case on infiltrative rectal adenocarcinoma diagnosed in a dog.Case: A 7-year-old male dog representative of the Fila Brasileiro breed was presented to the Veterinary Hospital of University Federal Rural of Amazonia, with history of hyperthermia, anorexia, apathy and tenesmus. Imaging examinations depicted prostatomegaly. Exploratory laparotomy was performed and ...

Research paper thumbnail of Tuberculosis Caused by Mycobacterium tuberculosis Complex in a Captive Tapir (Tapirus terrestris)

Acta Scientiae Veterinariae

Background: Tuberculosis is an infectious disease caused by the Mycobacterium tuberculosis comple... more Background: Tuberculosis is an infectious disease caused by the Mycobacterium tuberculosis complex has already been identified in a great number of wild species. Captive animals may have a potential source for zoonoses, because is related to factors such as cohabitation, direct contact with the public, presence of biological vectors, nutritional deficiencies, absence of sanitary barriers, precautionary hygienic measures and sanitary vigilance. In Brazil, there is little information on tuberculosis in captivity animals, and little attention is given to the risks of disease transmission from humans. The aim of this study was to report the first diagnosed case of tuberculosis Tapirus terrestris in Brazilian Amazon region.Case: One Tapirus terrestris was kept by local zoobotanic foundation in city of Marabá, Southeast of Pará state, Brazilian Amazon, and became ill. Physical examination revealed cough, sneezing, nasal outflow, dyspnea, hyperthermia and lethargy, leading to death. Necrop...

Research paper thumbnail of Ocorrência e Aspectos Macro e Microscópicos Do Cisto De Inclusão Da Serosa Uterina Em Cadelas e Gatas

Archives of Veterinary Science, 2015

Objetivou-se relatar a ocorrência de cistos de inclusão da serosa uterina em cadelas e gatas, bem... more Objetivou-se relatar a ocorrência de cistos de inclusão da serosa uterina em cadelas e gatas, bem como descrever seus aspectos macroscópicos e microscópicos. Foram utilizados 200 úteros (100 cadelas e 100 gatas) procedentes de ovariosalpingohisterectomia eletiva. As peças foram fixadas inteiras em formaldeído a 10% tamponado, seguindo-se ao processamento histológico de rotina, com as lâminas coradas pela hematoxilina e eosina. Os animais de estudo foram classificados segundo a espécie, a faixa etária, a parição e o uso de contraceptivos. Os dados obtidos foram analisados pelo teste Qui-quadrado, com nível de significância de 5%. Dos úteros analisados 7% das cadelas e 1% das gatas apresentaram cistos de inclusão da serosa uterina, não havendo diferença estatística significativa para a faixa etária, a parição e o uso de contraceptivos interespécie. Macroscopicamente, foram observadas dilatações císticas únicas localizadas na região miometrial ou na superfície serosa do útero. Estes ci...

Research paper thumbnail of Spatial–Temporal Evolution of Soil Erosion in a Typical Mountainous Karst Basin in SW China, Based on GIS and RUSLE

Arabian Journal for Science and Engineering, 2015

In this study, we address the characteristics of the spatial and temporal evolution of soil erosi... more In this study, we address the characteristics of the spatial and temporal evolution of soil erosion in karstic mountainous area of Houzhai River Basin of southwest China. The study uses GIS, erodibility test results, actual surveys of soil types, a revised universal soil loss equation for estimating soil erosion in 1973, 1990, 2002, and 2013, and an analysis of the spatial and temporal distribution of the evolution law of soil erosion for almost 40 years. Results show the following: (1) The soil erosion modulus of the Houzhai River Basin exhibits a decrease from 222.72 t km−2 year−1 in 1973 to 103.82 t km−2 year−1 in 2013. (2) The intensity level of soil erosion changes across space. The most active area is mainly concentrated in the upstream peak cluster depression. Areas with a slope of 8°–25° account for 40.81 % of the total erosion contribution rate, indicating that these are the main areas where erosion occurs. (3) The global and universal classification standard of soil erosion shows that the Houzhai River Basin areas above 65 % do not undergo micro-erosion. The rocky desertification is a serious problem, which explains why grading standard soil erosion intensity is inapplicable to karst areas. (4) The average soil erosion modulus in the basin is generally low (i.e., below 1000 t km−2 year−1), which indicates strong karstification in the karst areas. This process can occur through subsurface pores, fissures, holes, and underground leakage phenomena, which underestimate the amount of soil erosion in the karst areas. A comprehensive analysis of a long time series reveals that soil erosion in the karst areas is a complex process which requires further detailed studies.

Research paper thumbnail of Study of Arboviruses in Philander opossum, Didelphis marsupialis and Nectomys rattus captured from forest fragments in the municipality of Belém, Pará, Brazil

Ciência Rural

ABSTRACT: Arboviruses are viruses that maintain their life cycle in the wild and are transmitted ... more ABSTRACT: Arboviruses are viruses that maintain their life cycle in the wild and are transmitted to vertebrate hosts by hematophagous diptera. They are zoonotic and can establish an enzootic cycle in the urban areas; in humans, the infection can manifest from being encephalitogenic to hemorrhagic. This study aimed to report the occurrence of arboviruses in mammals of the order Didelphimorphia and Rodentia, captured from the Amazon. Serum samples were subjected to hemagglutination inhibition test using a viral panel of 19 species of arboviruses that are known to occur in the Amazon. Altogether, 14 wild mammals, 12 of Philander opossum, 1 of Didelphis marsupialis, and 1 of Nectomys rattus were captured. Eight of these were reported to be seropositive for arboviruses (57.14%) with monotypic seroprevalence for the Eastern Equine Encephalitis Virus (n=1), the Ilheus Virus (n=2), and the Catu virus (n=4); 4 heterotypic responses were observed for Flavivirus and Orthobunyavirus. In conclus...

Research paper thumbnail of Cryptosporidium spp. e Giardia spp. em carnívoros silvestres do Estado do Pará Brasil

Revista Brasileira de Ciência Veterinária

O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes es... more O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes espécimes silvestres da ordem Carnívora de vida livre e de cativeiro procedentes de municípios do Estado do Pará. Coletou-se amostras fecais de 37 animais distintos (quatro de vida livre e 33 de cativeiro). Para pesquisa de Cryptosporidium spp. e Giardia spp. foram utilizados métodos microscópicos (direto e Kinyoun) e imunológico (RIDA ® QUICK Cryptosporidium/Giardia/Entamoeba Combi-N1722). Do total de amostras, 24,32% (9/37) foram positivas, correspondendo a 5,4% (2/37) para Cryptosporidium spp. e 18,91% (7/37) para Giardia spp., respectivamente. Nenhum animal apresentou infecção concomitante para os agentes. Cryptosporidium spp. e Giardia spp., são protozoários zoonóticos que representam um emergente problema de saúde pública. Esses parasitos podem apresentar elevada frequência em regiões em que as condições de saneamento básico são precárias, promovendo surtos de diarreia em animais domésticos, silvestres e no homen. Mamíferos silvestres, como os carnívoros, são susceptíveis à contaminação por enteroparasitas presentes tanto no habitat natural como em cativeiro. Portanto, a pesquisa comprova a presença desses protozoários em carnívoros silvestres, tanto mantidos em criatórios como nos de vida livre no Estado do Pará, considerando-se que esses animais podem atuar como fontes de infecção para o homem, para outros animais e para o meio ambiente.

Research paper thumbnail of Cryptosporidium spp. e Giardia spp. em carnívoros silvestres do Estado do Pará Brasil

Revista Brasileira de Ciência Veterinária, 2019

O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes es... more O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes espécimes silvestres da ordem Carnívora de vida livre e de cativeiro procedentes de municípios do Estado do Pará. Coletou-se amostras fecais de 37 animais distintos (quatro de vida livre e 33 de cativeiro). Para pesquisa de Cryptosporidium spp. e Giardia spp. foram utilizados métodos microscópicos (direto e Kinyoun) e imunológico (RIDA ® QUICK Cryptosporidium/Giardia/Entamoeba Combi-N1722). Do total de amostras, 24,32% (9/37) foram positivas, correspondendo a 5,4% (2/37) para Cryptosporidium spp. e 18,91% (7/37) para Giardia spp., respectivamente. Nenhum animal apresentou infecção concomitante para os agentes. Cryptosporidium spp. e Giardia spp., são protozoários zoonóticos que representam um emergente problema de saúde pública. Esses parasitos podem apresentar elevada frequência em regiões em que as condições de saneamento básico são precárias, promovendo surtos de diarreia em animais domésticos, silvestres e no homen. Mamíferos silvestres, como os carnívoros, são susceptíveis à contaminação por enteroparasitas presentes tanto no habitat natural como em cativeiro. Portanto, a pesquisa comprova a presença desses protozoários em carnívoros silvestres, tanto mantidos em criatórios como nos de vida livre no Estado do Pará, considerando-se que esses animais podem atuar como fontes de infecção para o homem, para outros animais e para o meio ambiente.

Research paper thumbnail of Adenocarcinoma Pancreático Diferenciado De Tecido Ectópico Em Felino –Relato De Caso

INVESTIGAÇÃO, Sep 12, 2018

Research paper thumbnail of Leptospirosis, bovine viral diarrhea and infectious bovine rhinotracheitis: prevalence in Colombian cattle and buffaloes

Acta Scientiarum. Animal Sciences

One of the limiting factors of productive efficiency in cattle and buffalo herds is related to th... more One of the limiting factors of productive efficiency in cattle and buffalo herds is related to the high prevalence of infectious diseases which affect reproduction. The aim of this study was to determine the prevalence of anti-Leptospira antibodies for bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) in bovine and buffalo herds in Colombia. Blood serum samples were collected from 1100 buffaloes and 1000 cattle. The ELISA technique was used to detect antibodies against BVDV and BoHV-1, and the microscopic agglutination technique to detect anti-Leptospira antibodies. The prevalence of anti-Leptospira antibodies and of BVDV and BoHV-1 in bovine samples was observed in 16, 39.7, and 65% of animals, respectively, while the positivity in samples for the same antibodies in buffalos was detected in 18.7, 27.5 and 51.5%, respectively. Exposure of cattle and buffaloes to BoHV-1 was positively associated with age, higher prevalence rates were observed in older ages. Se...

Research paper thumbnail of Estudo de Arbovírus em Philander opossum, Didelphis marsupialis e Nectomys rattus capturados em fragmentos florestais no município de Belém, Pará

Research paper thumbnail of Innate immunity gene expression profiles in conjunctival membrane biopsies from Amazonian buffalo

Acta Amazonica, 2022

Domestic buffalo production plays an economically important role in the Brazilian Amazon, but the... more Domestic buffalo production plays an economically important role in the Brazilian Amazon, but they are susceptible to many diseases favored by the tropical climate and annually flooded habitats, including ocular diseases. In this context, it is important to select genotypes that maximize innate ocular immunity in Amazonian herds. We aimed to characterise, for the first time, gene expression profiles of the innate immune system in the conjunctival membrane of buffalo. Ocular conjunctival tissue samples were collected from 60 clinically healthy slaughtered animals in the northern Brazilian state of Amapá. The samples were histologically processed for classification into three groups according to the quantitative degree of lymphoid tissue associated with the conjunctiva (discrete, G1; slight, G2; and moderate, G3 presence of lymphoid tissue). RT-PCR was used to quantify gene expression of inflammatory cytokine (IL6, IL10, TNFA, IFNG), Toll-like receptor 4 (TLR4), and Defensin beta 110 ...

Research paper thumbnail of Experiencias de la aplicación de fitorreguladores para mejorar la calidad de la uva

Research paper thumbnail of La mejora de la calidad en los servicios de la universidad: experiencias desarrolladas en la secretaría de un departameto universitario

Materiales Para La Calidad Actas De Las Ii Jornadas Andaluzas De Calidad En La Ensenanza Universitaria Desarrollo De Planes De Calidad Para La Universidad Vol 1 2000 Isbn 84 86849 21 7 Pags 121 130, 2000

Research paper thumbnail of Diagnóstico de babesia caballi em éguas doadoras e receptoras de embrião pela técnica de reação em cadeia polimerase

Research paper thumbnail of Reações teciduais induzidas pela abraçadeira autoestática de náilon em ligaduras de pedículos ovarianos

Biotemas

Objetivou-se avaliar as reações teciduais induzidas pela abraçadeira autoestática de náilon na oc... more Objetivou-se avaliar as reações teciduais induzidas pela abraçadeira autoestática de náilon na oclusão dos pedículos ovarianos, por meio de avaliação macroscópica e histológica. Utilizaram-se 45 fêmeas caninas, sem raça definida, clinicamente sadias, com idade média de 31,11 ± 14,26 meses e peso médio de 11,26 ± 4,7 kg. Todas foram submetidas à ovariosalpingohisterectomia pela técnica minimamente invasiva. Os animais foram divididos aleatoriamente em três grupos com 15 animais cada, que foram avaliados no pós-cirúrgico imediato e aos 30 (G1), 60 (G2) e 90 dias (G3) de pós-operatório. Ao exame histológico dos pedículos contendo as abraçadeiras foi observada, no 30º dia de pós-operatório, reação inflamatória crônica com macrófagos, células gigantes e fibroplasia. No 60º dia, tecido conjuntivo totalmente organizado e em modelação, e presença de linfócitos e polimorfonucleares. No 90º dia de pós-operatório, tecido conjuntivo maduro totalmente organizado com áreas em modelação, macrófago...

Research paper thumbnail of Achados Anatomopatológicos De Toxoplasma Sp. Em Lagothrix Lagotricha Da Região Amazônica

Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmissio... more Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmission, and a biological cycle of the heteroxenic type, capable of infecting a wide variety of homeothermic animals, including non-human primates and human. The evolution of the disease occurs in an acute and chronic form, depending on the parasite-host relationship, the physiological condition of the infected and virulence, which can lead to death. In this report, the autopsy of three specimens of Lagothrix lagotricha was performed. In the necroscopic examination of the specimens, pulmonary edema and an increase in the size of the mesenteric and submandibular lymph nodes, liver and spleen were observed. Tissue samples were collected and fixed in 10% buffered formaldehyde, processed and analyzed. Histopathology identified structures of T. gondii in the forms of cystzoites in lymph node tissues, cardiac musculature, lungs, spleen and liver, and tachyzoites in liver tissue. It is concluded that...

Research paper thumbnail of Serological markers for hepatitis a among captive and free-living wild mammals in the State of Pará, Brazil

Semina-ciencias Agrarias, 2021

The hepatitis A virus (HAV, Hepatovirus A) in humans has a relevant impact on public health, espe... more The hepatitis A virus (HAV, Hepatovirus A) in humans has a relevant impact on public health, especially in geographic regions with poor basic sanitation, such as the Brazilian Amazon. Isolates related to hepatoviruses have been described in non human primates, chiropterans, marsupials, rodents, marmots, shrews, and terrestrial hedgehogs. However, the circulation of these viruses in the Amazonian mammal fauna remains unexplored. This study aimed to evaluate the prevalence of antibodies against this hepatovirus in captive and free-living wild mammals belonging to the orders Didelphimorphia, Primates, Carnivora, and Artiodactyla. Serum samples from 71 animals, from three municipalities in the State of Pará (Belém, Santarém, and CapitãoPoço) were tested for total anti-HAV and anti-HAV IgM through enzyme-linked immunosorbent assay (ELISA). Total anti-HAV antibodies were detected in 29.5% (21/71) of non-human primates, 8.4% (6/71) of carnivores, 1 Student of the Doctoral Course of the Pós...

Research paper thumbnail of Cryptosporidium spp. oocysts and Giardia spp. cysts in coatis (Nasua nasua L. 1766) from Pará, Brazil

Acta Veterinaria Brasilica, Sep 30, 2017

Article history Zoonotic enteroparasitosis represent an important public health problem, and spec... more Article history Zoonotic enteroparasitosis represent an important public health problem, and species of protozoa such as Cryptosporidium spp. and Giardia spp. can reach high frequencies in regions where basic sanitation conditions are poor, which promotes outbreaks of diarrhea in humans and domestic and wild animals. Wild mammals such as the South American coati (Nasua nasua) feed on insects present in the soil, fruits, and small vertebrates, and are susceptible to contamination by enteroparasites present in the environmental niche. The aim of the present study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in N. nasua from a region in the Brazilian Amazon. Fecal samples of 27 coatis two from free-living and 25 from captivity were collected in three different municipalities in the state of Pará, Brazil. The search for Giardia spp. and Cryptosporidium spp. in the collected samples were performed using the direct and Kinyoun methods, respectively, and a commercial immunological test (RIDA®QUICK Cryptosporidium/Giardia Combi. ®R-Biopharm) was used to detect antigens from both parasites. Cryptosporidium spp. oocysts were found in 11.1% (3/27) of the samples; one from a free-living animal and two from captive animals. Giardia spp. cysts were found in 11.1% (3/27) of the samples, all from captive animals. This is the first report of infection by these protozoans in this coati's species in the North region of Brazil; the South American coati may be participating as maintainers and disseminators of infectious agents to the environment and other hosts.

Research paper thumbnail of Uterine adenomyosis in bitches and queens: occurrence and histopathological aspects

Acta Veterinaria Brasilica

Article history The goal of this study was to report the occurrence and histopathological aspects... more Article history The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.

Research paper thumbnail of Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil

Semina: Ciências Agrárias

Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the ... more Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was ...

Research paper thumbnail of Infiltrative Rectal Adenocarcinoma in a Dog

Acta Scientiae Veterinariae

Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological... more Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological type found. This neoplasm presents slow growth and high capacity of causing metastasis. Histologically speaking, neoplasm cells can present solid, tubular, papillary arrangement and note amorphous extra-cellular material. Clinically observed tenesmus, diarrhea, dyskinesia, hematochezia, mane, protrusion of the anus, weight loss, anorexia. The occurrence and clinicopathological aspects of tumors in dogs’ gastrointestinal tract, the rectal segment, remains poorly understood. Accordingly, the aim of the present study is to report a case on infiltrative rectal adenocarcinoma diagnosed in a dog.Case: A 7-year-old male dog representative of the Fila Brasileiro breed was presented to the Veterinary Hospital of University Federal Rural of Amazonia, with history of hyperthermia, anorexia, apathy and tenesmus. Imaging examinations depicted prostatomegaly. Exploratory laparotomy was performed and ...

Research paper thumbnail of Tuberculosis Caused by Mycobacterium tuberculosis Complex in a Captive Tapir (Tapirus terrestris)

Acta Scientiae Veterinariae

Background: Tuberculosis is an infectious disease caused by the Mycobacterium tuberculosis comple... more Background: Tuberculosis is an infectious disease caused by the Mycobacterium tuberculosis complex has already been identified in a great number of wild species. Captive animals may have a potential source for zoonoses, because is related to factors such as cohabitation, direct contact with the public, presence of biological vectors, nutritional deficiencies, absence of sanitary barriers, precautionary hygienic measures and sanitary vigilance. In Brazil, there is little information on tuberculosis in captivity animals, and little attention is given to the risks of disease transmission from humans. The aim of this study was to report the first diagnosed case of tuberculosis Tapirus terrestris in Brazilian Amazon region.Case: One Tapirus terrestris was kept by local zoobotanic foundation in city of Marabá, Southeast of Pará state, Brazilian Amazon, and became ill. Physical examination revealed cough, sneezing, nasal outflow, dyspnea, hyperthermia and lethargy, leading to death. Necrop...

Research paper thumbnail of Ocorrência e Aspectos Macro e Microscópicos Do Cisto De Inclusão Da Serosa Uterina Em Cadelas e Gatas

Archives of Veterinary Science, 2015

Objetivou-se relatar a ocorrência de cistos de inclusão da serosa uterina em cadelas e gatas, bem... more Objetivou-se relatar a ocorrência de cistos de inclusão da serosa uterina em cadelas e gatas, bem como descrever seus aspectos macroscópicos e microscópicos. Foram utilizados 200 úteros (100 cadelas e 100 gatas) procedentes de ovariosalpingohisterectomia eletiva. As peças foram fixadas inteiras em formaldeído a 10% tamponado, seguindo-se ao processamento histológico de rotina, com as lâminas coradas pela hematoxilina e eosina. Os animais de estudo foram classificados segundo a espécie, a faixa etária, a parição e o uso de contraceptivos. Os dados obtidos foram analisados pelo teste Qui-quadrado, com nível de significância de 5%. Dos úteros analisados 7% das cadelas e 1% das gatas apresentaram cistos de inclusão da serosa uterina, não havendo diferença estatística significativa para a faixa etária, a parição e o uso de contraceptivos interespécie. Macroscopicamente, foram observadas dilatações císticas únicas localizadas na região miometrial ou na superfície serosa do útero. Estes ci...

Research paper thumbnail of Spatial–Temporal Evolution of Soil Erosion in a Typical Mountainous Karst Basin in SW China, Based on GIS and RUSLE

Arabian Journal for Science and Engineering, 2015

In this study, we address the characteristics of the spatial and temporal evolution of soil erosi... more In this study, we address the characteristics of the spatial and temporal evolution of soil erosion in karstic mountainous area of Houzhai River Basin of southwest China. The study uses GIS, erodibility test results, actual surveys of soil types, a revised universal soil loss equation for estimating soil erosion in 1973, 1990, 2002, and 2013, and an analysis of the spatial and temporal distribution of the evolution law of soil erosion for almost 40 years. Results show the following: (1) The soil erosion modulus of the Houzhai River Basin exhibits a decrease from 222.72 t km−2 year−1 in 1973 to 103.82 t km−2 year−1 in 2013. (2) The intensity level of soil erosion changes across space. The most active area is mainly concentrated in the upstream peak cluster depression. Areas with a slope of 8°–25° account for 40.81 % of the total erosion contribution rate, indicating that these are the main areas where erosion occurs. (3) The global and universal classification standard of soil erosion shows that the Houzhai River Basin areas above 65 % do not undergo micro-erosion. The rocky desertification is a serious problem, which explains why grading standard soil erosion intensity is inapplicable to karst areas. (4) The average soil erosion modulus in the basin is generally low (i.e., below 1000 t km−2 year−1), which indicates strong karstification in the karst areas. This process can occur through subsurface pores, fissures, holes, and underground leakage phenomena, which underestimate the amount of soil erosion in the karst areas. A comprehensive analysis of a long time series reveals that soil erosion in the karst areas is a complex process which requires further detailed studies.

Research paper thumbnail of Study of Arboviruses in Philander opossum, Didelphis marsupialis and Nectomys rattus captured from forest fragments in the municipality of Belém, Pará, Brazil

Ciência Rural

ABSTRACT: Arboviruses are viruses that maintain their life cycle in the wild and are transmitted ... more ABSTRACT: Arboviruses are viruses that maintain their life cycle in the wild and are transmitted to vertebrate hosts by hematophagous diptera. They are zoonotic and can establish an enzootic cycle in the urban areas; in humans, the infection can manifest from being encephalitogenic to hemorrhagic. This study aimed to report the occurrence of arboviruses in mammals of the order Didelphimorphia and Rodentia, captured from the Amazon. Serum samples were subjected to hemagglutination inhibition test using a viral panel of 19 species of arboviruses that are known to occur in the Amazon. Altogether, 14 wild mammals, 12 of Philander opossum, 1 of Didelphis marsupialis, and 1 of Nectomys rattus were captured. Eight of these were reported to be seropositive for arboviruses (57.14%) with monotypic seroprevalence for the Eastern Equine Encephalitis Virus (n=1), the Ilheus Virus (n=2), and the Catu virus (n=4); 4 heterotypic responses were observed for Flavivirus and Orthobunyavirus. In conclus...

Research paper thumbnail of Cryptosporidium spp. e Giardia spp. em carnívoros silvestres do Estado do Pará Brasil

Revista Brasileira de Ciência Veterinária

O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes es... more O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes espécimes silvestres da ordem Carnívora de vida livre e de cativeiro procedentes de municípios do Estado do Pará. Coletou-se amostras fecais de 37 animais distintos (quatro de vida livre e 33 de cativeiro). Para pesquisa de Cryptosporidium spp. e Giardia spp. foram utilizados métodos microscópicos (direto e Kinyoun) e imunológico (RIDA ® QUICK Cryptosporidium/Giardia/Entamoeba Combi-N1722). Do total de amostras, 24,32% (9/37) foram positivas, correspondendo a 5,4% (2/37) para Cryptosporidium spp. e 18,91% (7/37) para Giardia spp., respectivamente. Nenhum animal apresentou infecção concomitante para os agentes. Cryptosporidium spp. e Giardia spp., são protozoários zoonóticos que representam um emergente problema de saúde pública. Esses parasitos podem apresentar elevada frequência em regiões em que as condições de saneamento básico são precárias, promovendo surtos de diarreia em animais domésticos, silvestres e no homen. Mamíferos silvestres, como os carnívoros, são susceptíveis à contaminação por enteroparasitas presentes tanto no habitat natural como em cativeiro. Portanto, a pesquisa comprova a presença desses protozoários em carnívoros silvestres, tanto mantidos em criatórios como nos de vida livre no Estado do Pará, considerando-se que esses animais podem atuar como fontes de infecção para o homem, para outros animais e para o meio ambiente.

Research paper thumbnail of Cryptosporidium spp. e Giardia spp. em carnívoros silvestres do Estado do Pará Brasil

Revista Brasileira de Ciência Veterinária, 2019

O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes es... more O presente estudo investigou a ocorrência de Cryptosporidium spp. e Giardia spp. em diferentes espécimes silvestres da ordem Carnívora de vida livre e de cativeiro procedentes de municípios do Estado do Pará. Coletou-se amostras fecais de 37 animais distintos (quatro de vida livre e 33 de cativeiro). Para pesquisa de Cryptosporidium spp. e Giardia spp. foram utilizados métodos microscópicos (direto e Kinyoun) e imunológico (RIDA ® QUICK Cryptosporidium/Giardia/Entamoeba Combi-N1722). Do total de amostras, 24,32% (9/37) foram positivas, correspondendo a 5,4% (2/37) para Cryptosporidium spp. e 18,91% (7/37) para Giardia spp., respectivamente. Nenhum animal apresentou infecção concomitante para os agentes. Cryptosporidium spp. e Giardia spp., são protozoários zoonóticos que representam um emergente problema de saúde pública. Esses parasitos podem apresentar elevada frequência em regiões em que as condições de saneamento básico são precárias, promovendo surtos de diarreia em animais domésticos, silvestres e no homen. Mamíferos silvestres, como os carnívoros, são susceptíveis à contaminação por enteroparasitas presentes tanto no habitat natural como em cativeiro. Portanto, a pesquisa comprova a presença desses protozoários em carnívoros silvestres, tanto mantidos em criatórios como nos de vida livre no Estado do Pará, considerando-se que esses animais podem atuar como fontes de infecção para o homem, para outros animais e para o meio ambiente.