Marco Fragai - Academia.edu (original) (raw)
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Papers by Marco Fragai
Journal of Molecular Catalysis A: Chemical, 2003
In the general frame of a project aiming at screening candidate drugs against several different m... more In the general frame of a project aiming at screening candidate drugs against several different matrix metalloproteinases (MMP) to find a rationale for selectivity, the catalytic domain of MMP-12 (metalloelastase) has been expressed in E. coli strain BL21D3 and its production optimized to about 30 mg/dm 3 . The chosen construct spans residues 106-267 of the whole MMP-12 and contains two additional methionines at positions 104-105. This is at variance with the previously published constructs which span residues 99-279 [J. Mol. Biol. 312 743] and 100-262 [J. Mol. Biol. 312 (2001) 731], respectively.
Journal of Molecular Catalysis A: Chemical, 2003
In the general frame of a project aiming at screening candidate drugs against several different m... more In the general frame of a project aiming at screening candidate drugs against several different matrix metalloproteinases (MMP) to find a rationale for selectivity, the catalytic domain of MMP-12 (metalloelastase) has been expressed in E. coli strain BL21D3 and its production optimized to about 30 mg/dm 3 . The chosen construct spans residues 106-267 of the whole MMP-12 and contains two additional methionines at positions 104-105. This is at variance with the previously published constructs which span residues 99-279 [J. Mol. Biol. 312 743] and 100-262 [J. Mol. Biol. 312 (2001) 731], respectively.