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Papers by Marco Massironi
International Journal of Cosmetic Science, 2019
INTRODUCTION: We report on the preparation and efficacy of 10-hydroxystearic acid (HSA) that impr... more INTRODUCTION: We report on the preparation and efficacy of 10-hydroxystearic acid (HSA) that improves facial age spots and conspicuous pores. METHODS: The hydration of oleic acid into HSA was catalyzed by the oleate hydratase from Escherichia coli. Following treatment with HSA, collagen type I and type III was assessed in primary human dermal fibroblasts together with collagen type III, p53 protein levels and sunburn cells (SBC) after UVB irradiation (1 J cm À2) by immunohistochemistry on human ex vivo skin. UVB-induced expression of matrix metalloprotease-1 (MMP-1) was determined from full thickness skin by RT-qPCR. Modification of the fibroblast secretome by HSA was studied by mass-spectrometry-based proteomics. In a full-face, double blind, vehicle-controlled trial HSA was assessed for its effects on conspicuous facial pore size and degree of pigmentation of age spots in Caucasian women over an 8-week period. RESULTS: HSA was obtained in enantiomeric pure, high yield (≥80%). Collagen type I and type III levels were dose-dependently increased (96% and 244%; P < 0.01) in vitro and collagen type III in ex vivo skin by +57% (P < 0.01) by HSA. HSA also inhibited UVB-induced MMP-1 gene expression (83%; P < 0.01) and mitigated SBC induction (À34% vs. vehicle control) and reduced significantly UV-induced p53 up-regulation (À46% vs. vehicle control; P < 0.01) in irradiated skin. HSA modified the fibroblast secretome with significant increases in proteins associated with the WNT pathway that could reduce melanogenesis and proteins that could modify dermal fibroblast activity and keratinocyte differentiation to account for the alleviation of conspicuous pores. Docking studies in silico and EC50 determination in reporter gene assays (EC50 5.5 9 10 À6 M) identified HSA as a peroxisomal proliferator activated receptor-a (PPARa) agonist. Clinically, HSA showed a statistically significant decrease of surface and volume of skin pores (P < 0.05) after 8 weeks of application and age spots became significantly less pigmented than the surrounding skin (contrast, P < 0.05) after 4 weeks. CONCLUSION: HSA acts as a PPARa agonist to reduce the signs of age spots and conspicuous pores by significantly modulating the expression of p53, SBC, MMP-1 and collagen together with major changes in secreted proteins that modify keratinocyte, melanocyte and fibroblast cell behavior.
International Journal of Peptide Research and Therapeutics, 2019
Aging of skin manifests in loss of volume and firming due to degradation of extracellular matrix ... more Aging of skin manifests in loss of volume and firming due to degradation of extracellular matrix components such as collagen and hyaluronic acid leading to wrinkling and sagging. To counteract loss of facial volume and regain firmness, fillers like hyaluronic acid (HA) are commonplace in cosmetic dermatology. We developed a synthetic tripeptide tetradecyl aminobutyroylvalylaminobutyric acid urea trifluoroacetate with proven hyaluronic acid stimulating activity in vitro. This study investigated the filling and firming activity of the tripeptide. In vitro: Microtissue technology was used to construct spherical 3D-skin equivalents. These were exposed to a tripeptide solution and content of hyaluronic acid and its receptor CD44 were assessed using histological techniques. Skin tissue culture was used to assess HA expression ex vivo. A placebo controlled, randomized, in parallel groups study to assess the firming, filling, and moisturizing activity of the peptide product was designed. We recruited 30 female Caucasian volunteers age 40 to 60 per group. Product application was twice daily for 29 days. Skin volume, deformation and moisturization were measured. HA and CD44 content in skin were increased in vitro and ex vivo. In vivo, skin firming was improved by a significant decrease in cheek deformation, a significantly restored skin volume below the eyes, and significantly improved skin hydration as measured on the cheekbone. We show evidence that the tripeptide tetradecyl-diaminobutyroylvalyldiaminobutyric urea trifluoroacetate restores facial skin volume by stimulating HA synthesis. These results underline the anti-aging activity of this synthetic tripeptide.
Suggested are new extracts of microalgae selected from the group consisting of (i) Monodus sp. (i... more Suggested are new extracts of microalgae selected from the group consisting of (i) Monodus sp. (ii) Thalassiosira sp. (iii) Chaetoceros sp. and/or (iv) Chlorococcum sp. obtainable by treating said microalgae with a solvent selected from the group consisting of C1-C4 aliphatic alcohols, ethyl acetate, water or their mixtures, removing the dissolved extracts from the residues and recovering the pure extracts from the solvent. The extracts show excellent properties particularly in modulating the metabolism of human skin and air follicles.
Marine Biology, 2005
A large body of research has developed on male ornaments and their role in sexual selection while... more A large body of research has developed on male ornaments and their role in sexual selection while female ornaments have been less investigated. This study focused on the presence, function, and possible role of a sexually dimorphic nuptial trait as an indicator of female quality in a fish species presenting ''conventional'' sex roles: the yellow patch on the belly of Knipowitschia panizzae females. Aquarium spawning experiments demonstrated that this ornament is due to dermal pigments, is displayed only when the female is ready to spawn, and is switched off within minutes from the end of egg deposition. This sexual trait is variable in size among females and indicates female fecundity relative to her own body size. As a consequence, female yellow belly appears to be a reliable indicator of female quality as measured by current fecundity. Field data on natural nests highlights that males usually perform parental care on only one egg batch at a time. The modality of egg deposition suggests that males may be limited in their potential reproductive rates by environmental factors. Male limitations in egg care could favour male choosiness and the evolution of female nuptial displays.
International journal of cosmetic science, Jan 20, 2017
To examine the ability of an extract from traditional Chinese medicine, Polygonum multiflorum Rad... more To examine the ability of an extract from traditional Chinese medicine, Polygonum multiflorum Radix, to protect melanocyte viability from oxidative stress, a key mechanism in the initiation and progression of hair greying. To assess the antioxidant capacity of Polygonum multiflorum Radix extract, primary human foreskin melanocytes were treated with a commercially available Polygonum multiflorum Radix extract added to culture medium and exposed to hydrogen peroxide (H2 O2 ), using intracellular reactive oxygen species concentrations and glutathione/protein ratios as endpoints. To improve solubility for cosmetic uses, a new Polygonum multiflorum Radix extract was derived. As hair graying is the consequence of melanocyte disappearance in an oxidative stress environment, we checked if the anti-oxidant capacity of the new Polygonum multiflorum Radix extract could preserve melanocyte viability in response to H2 O2 -induced oxidative stress, and preserve pigmentation within ex vivo human h...
L'invention concerne de nouveaux extraits de micro-algues selectionnes dans le groupe constit... more L'invention concerne de nouveaux extraits de micro-algues selectionnes dans le groupe constitue par (i) Monodus sp., (ii) Thalassiosira sp., (iii) Chaetoceros sp. et/ou (iv) Chlorococcum sp., pouvant etre obtenus par traitement des micro-algues au moyen d'un solvant selectionne dans le groupe constitue par des alcools aliphatiques C 1 -C 4 , de l'acetate d'ethyle, de l'eau ou des melanges de ceux-ci, par separation des extraits dissous des residus et par recuperation des extraits purs du solvant. Les extraits selon l'invention presentent d'excellentes proprietes, notamment en ce qui concerne la modulation du metabolisme de la peau et des follicules pileux humains.
International Journal of Cosmetic Science, 2019
INTRODUCTION: We report on the preparation and efficacy of 10-hydroxystearic acid (HSA) that impr... more INTRODUCTION: We report on the preparation and efficacy of 10-hydroxystearic acid (HSA) that improves facial age spots and conspicuous pores. METHODS: The hydration of oleic acid into HSA was catalyzed by the oleate hydratase from Escherichia coli. Following treatment with HSA, collagen type I and type III was assessed in primary human dermal fibroblasts together with collagen type III, p53 protein levels and sunburn cells (SBC) after UVB irradiation (1 J cm À2) by immunohistochemistry on human ex vivo skin. UVB-induced expression of matrix metalloprotease-1 (MMP-1) was determined from full thickness skin by RT-qPCR. Modification of the fibroblast secretome by HSA was studied by mass-spectrometry-based proteomics. In a full-face, double blind, vehicle-controlled trial HSA was assessed for its effects on conspicuous facial pore size and degree of pigmentation of age spots in Caucasian women over an 8-week period. RESULTS: HSA was obtained in enantiomeric pure, high yield (≥80%). Collagen type I and type III levels were dose-dependently increased (96% and 244%; P < 0.01) in vitro and collagen type III in ex vivo skin by +57% (P < 0.01) by HSA. HSA also inhibited UVB-induced MMP-1 gene expression (83%; P < 0.01) and mitigated SBC induction (À34% vs. vehicle control) and reduced significantly UV-induced p53 up-regulation (À46% vs. vehicle control; P < 0.01) in irradiated skin. HSA modified the fibroblast secretome with significant increases in proteins associated with the WNT pathway that could reduce melanogenesis and proteins that could modify dermal fibroblast activity and keratinocyte differentiation to account for the alleviation of conspicuous pores. Docking studies in silico and EC50 determination in reporter gene assays (EC50 5.5 9 10 À6 M) identified HSA as a peroxisomal proliferator activated receptor-a (PPARa) agonist. Clinically, HSA showed a statistically significant decrease of surface and volume of skin pores (P < 0.05) after 8 weeks of application and age spots became significantly less pigmented than the surrounding skin (contrast, P < 0.05) after 4 weeks. CONCLUSION: HSA acts as a PPARa agonist to reduce the signs of age spots and conspicuous pores by significantly modulating the expression of p53, SBC, MMP-1 and collagen together with major changes in secreted proteins that modify keratinocyte, melanocyte and fibroblast cell behavior.
International Journal of Peptide Research and Therapeutics, 2019
Aging of skin manifests in loss of volume and firming due to degradation of extracellular matrix ... more Aging of skin manifests in loss of volume and firming due to degradation of extracellular matrix components such as collagen and hyaluronic acid leading to wrinkling and sagging. To counteract loss of facial volume and regain firmness, fillers like hyaluronic acid (HA) are commonplace in cosmetic dermatology. We developed a synthetic tripeptide tetradecyl aminobutyroylvalylaminobutyric acid urea trifluoroacetate with proven hyaluronic acid stimulating activity in vitro. This study investigated the filling and firming activity of the tripeptide. In vitro: Microtissue technology was used to construct spherical 3D-skin equivalents. These were exposed to a tripeptide solution and content of hyaluronic acid and its receptor CD44 were assessed using histological techniques. Skin tissue culture was used to assess HA expression ex vivo. A placebo controlled, randomized, in parallel groups study to assess the firming, filling, and moisturizing activity of the peptide product was designed. We recruited 30 female Caucasian volunteers age 40 to 60 per group. Product application was twice daily for 29 days. Skin volume, deformation and moisturization were measured. HA and CD44 content in skin were increased in vitro and ex vivo. In vivo, skin firming was improved by a significant decrease in cheek deformation, a significantly restored skin volume below the eyes, and significantly improved skin hydration as measured on the cheekbone. We show evidence that the tripeptide tetradecyl-diaminobutyroylvalyldiaminobutyric urea trifluoroacetate restores facial skin volume by stimulating HA synthesis. These results underline the anti-aging activity of this synthetic tripeptide.
Suggested are new extracts of microalgae selected from the group consisting of (i) Monodus sp. (i... more Suggested are new extracts of microalgae selected from the group consisting of (i) Monodus sp. (ii) Thalassiosira sp. (iii) Chaetoceros sp. and/or (iv) Chlorococcum sp. obtainable by treating said microalgae with a solvent selected from the group consisting of C1-C4 aliphatic alcohols, ethyl acetate, water or their mixtures, removing the dissolved extracts from the residues and recovering the pure extracts from the solvent. The extracts show excellent properties particularly in modulating the metabolism of human skin and air follicles.
Marine Biology, 2005
A large body of research has developed on male ornaments and their role in sexual selection while... more A large body of research has developed on male ornaments and their role in sexual selection while female ornaments have been less investigated. This study focused on the presence, function, and possible role of a sexually dimorphic nuptial trait as an indicator of female quality in a fish species presenting ''conventional'' sex roles: the yellow patch on the belly of Knipowitschia panizzae females. Aquarium spawning experiments demonstrated that this ornament is due to dermal pigments, is displayed only when the female is ready to spawn, and is switched off within minutes from the end of egg deposition. This sexual trait is variable in size among females and indicates female fecundity relative to her own body size. As a consequence, female yellow belly appears to be a reliable indicator of female quality as measured by current fecundity. Field data on natural nests highlights that males usually perform parental care on only one egg batch at a time. The modality of egg deposition suggests that males may be limited in their potential reproductive rates by environmental factors. Male limitations in egg care could favour male choosiness and the evolution of female nuptial displays.
International journal of cosmetic science, Jan 20, 2017
To examine the ability of an extract from traditional Chinese medicine, Polygonum multiflorum Rad... more To examine the ability of an extract from traditional Chinese medicine, Polygonum multiflorum Radix, to protect melanocyte viability from oxidative stress, a key mechanism in the initiation and progression of hair greying. To assess the antioxidant capacity of Polygonum multiflorum Radix extract, primary human foreskin melanocytes were treated with a commercially available Polygonum multiflorum Radix extract added to culture medium and exposed to hydrogen peroxide (H2 O2 ), using intracellular reactive oxygen species concentrations and glutathione/protein ratios as endpoints. To improve solubility for cosmetic uses, a new Polygonum multiflorum Radix extract was derived. As hair graying is the consequence of melanocyte disappearance in an oxidative stress environment, we checked if the anti-oxidant capacity of the new Polygonum multiflorum Radix extract could preserve melanocyte viability in response to H2 O2 -induced oxidative stress, and preserve pigmentation within ex vivo human h...
L'invention concerne de nouveaux extraits de micro-algues selectionnes dans le groupe constit... more L'invention concerne de nouveaux extraits de micro-algues selectionnes dans le groupe constitue par (i) Monodus sp., (ii) Thalassiosira sp., (iii) Chaetoceros sp. et/ou (iv) Chlorococcum sp., pouvant etre obtenus par traitement des micro-algues au moyen d'un solvant selectionne dans le groupe constitue par des alcools aliphatiques C 1 -C 4 , de l'acetate d'ethyle, de l'eau ou des melanges de ceux-ci, par separation des extraits dissous des residus et par recuperation des extraits purs du solvant. Les extraits selon l'invention presentent d'excellentes proprietes, notamment en ce qui concerne la modulation du metabolisme de la peau et des follicules pileux humains.