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Papers by Marco Vincenzo Patruno

Research paper thumbnail of Plasma treatment of sheep skin woods in veterinary medicine

Research paper thumbnail of Expression of IGF-II during development of Sparus aurata

Research paper thumbnail of “Characterization, differentiation and cryopreservation of equine peripheral blood derived mesenchymal stem cells (ePB-MSC)”

Research paper thumbnail of Effects of short- and long-term swimming on myostatin expression in red and white gastrocnemius muscle and heart muscle of the rat

Research paper thumbnail of Expression of Tgf-beta in the regenerative process of crinoid echinoderms

Research paper thumbnail of Effect of MLS® laser therapy for the treatment of experimentally induced acute tendinopathy in sheep – a preliminary study

Research paper thumbnail of Contractile properties of different types of dog muscle fibres

Research paper thumbnail of Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do

Frontiers in Bioengineering and Biotechnology, 2020

Research paper thumbnail of The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence

Research in Veterinary Science, 2019

Research paper thumbnail of Investigations of the corneal epithelium in Veterinary Medicine: State of the art on corneal stem cells found in different mammalian species and their putative application

Research in Veterinary Science, 2018

Research paper thumbnail of Muscle spindles of the rat sternomastoid muscle

European Journal of Translational Myology, 2018

The sternomastoid (SM) muscle in rodents presents a peculiar distribution of fiber types with a s... more The sternomastoid (SM) muscle in rodents presents a peculiar distribution of fiber types with a steep gradient from the ventral, superficial, white portion to the dorsal, deep, red region, where muscle spindles are restricted. Cross section of the medial longitudinal third of the rat SM contains around 10,000 muscle fibers with a mean diameter of 51.28±12.62 (μm +/- SD). Transverse sections stained by Succinate Dehydrogenase (SDH) reaction clearly presents two distinct regions: the dorsal deep red portion encompassing a 40% cross section area contains a high percentage of packed SDH-positive muscle fibers, and the ventral superficial region which contains mainly SDH-negative muscle fibers. Indeed, the ventral superficial region of the rat SM muscle contains mainly fast 2B muscle fibers. These acidic ATPase pH 4.3-negative and SDH-negative 2B muscle fibers are the largest of the SM muscle, while the acidic ATPase pH 4.3-positive and SDH-positive Type 1 muscle fibers are the smallest....

Research paper thumbnail of The fusion of MyoD transcription factor with the TAT peptide induces, in conditioned medium, equine stem cells towards a myogenic lineage

Annals of Anatomy - Anatomischer Anzeiger, 2016

Research paper thumbnail of CIR-Myo News: Abstracts of the 2015 Spring Padua Muscle Days

European Journal of Translational Myology, 2015

Research paper thumbnail of Might the masson trichrome stain be considered a useful method for categorizing experimental tendon lesions?

Histology and histopathology, Jan 3, 2015

Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they ... more Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subjective, and therefore a reliable grading method would be an improvement. The main purpose of this study was to assess qualitatively the histological pattern of Masson trichrome stain in healthy and injured tendons; indeed, the known "paradox" of Masson staining was used to create an evaluation for the matrix of tendons, following experimental lesions and natural repair processes. A statistically significant difference of aniline-staining between healthy and lesioned tendons was observed. Overall, we think that the Masson staining might be regarded as an informative tool in discerning the collagen spatial arrangement and therefore the histological characteristic...

Research paper thumbnail of Effect of TAT proteins on the differentiative pattern of equine mesenchymal stromal cells

Research paper thumbnail of In vitro and in vivo biocompatibility of marine-collagen biomimetic matrices: innovative applications for tendon and skin regenerative medicine

Research paper thumbnail of New regenerative strategies for tendon repair and regeneration

Annals of Anatomy - Anatomischer Anzeiger, 2014

Research paper thumbnail of 2B Myosin Heavy Chain Isoform Expression in Bovine Skeletal Muscle

Veterinary Research Communications, 2004

Research paper thumbnail of Analysis of neuromuscular junctions and effects of anabolic steroid administration in the SOD1G93A mouse model of ALS

Molecular and Cellular Neuroscience, 2012

Several lines of evidence indicate that neuromuscular junction (NMJ) destruction and disassembly ... more Several lines of evidence indicate that neuromuscular junction (NMJ) destruction and disassembly is an early phenomenon in amyotrophic lateral sclerosis (ALS). Here we analyzed by confocal and electron microscopy the NMJ structure in the diaphragm of SOD1G93A mice at symptom onset. In these mice, which provide a model for familial ALS, diaphragm denervation (~50%) as well as gastrocnemius denervation (~40%) was found. In addition, the size of the synaptic vesicle pool was reduced and alterations of mitochondria were observed in approximately 40% of the remaining presynaptic terminals. Chronic treatment of SOD1G93A mice with the anabolic steroid nandrolone during the presymptomatic stage preserved the diaphragm muscle mass and features indicative of synaptic activity. These features were represented by the number of vesicles docked within 200 nm from the presynaptic membrane and area of acetylcholine receptor clusters. Structural preservation of mitochondria was documented in presynaptic terminals. However, innervation of diaphragm muscle fibers was only slightly increased in nandrolone-treated SOD1-mutant mice. Altogether the results point out and define fine structural alterations of diaphragm NMJs in the murine model of familial ALS at symptom onset, and indicate that nandrolone may prevent or delay structural alterations in NMJ mitochondria and stimulate presynaptic activity but does not prevent muscle denervation during the disease.

Research paper thumbnail of PCB exposure and regeneration in crinoids (Echinodermata)

Marine Ecology Progress Series, 2001

Echinoderm regeneration provides a convenient and tractable test to monitor the effects of persis... more Echinoderm regeneration provides a convenient and tractable test to monitor the effects of persistent micropollutants on the developmental physiology of marine animals. Regeneration involves crucial and sensitive biological processes (cell proliferation, morphogenesis, differentiation, tissue renewal) that make it an ideal tool to assess micropollutants at the tissue and cellular level. Our results provide evidence that the regenerative response in the crinoid Antedon mediterranea is especially sensitive to PCBs and that exposure to these chemicals induces significant variations in timing and modality of arm regeneration. Prolonged exposure to low concentrations of Aroclor ® 1260 (14 ng l-1 of total PCBs), typical of moderately polluted coastal zones, results in abnormal arm growth in terms of both gross morphology and microscopic anatomy. In terms of tissue/cellular aspects the main modifications are: accelerated growth of the regenerate; massive cell migration/proliferation; hypertrophic development of the coelomic canals; and, extensive rearrangement of differentiated tissues of the stump. The anomalies observed in the developmental regenerative processes appear to be compatible with a pattern of pseudo-endocrine activities.

Research paper thumbnail of Plasma treatment of sheep skin woods in veterinary medicine

Research paper thumbnail of Expression of IGF-II during development of Sparus aurata

Research paper thumbnail of “Characterization, differentiation and cryopreservation of equine peripheral blood derived mesenchymal stem cells (ePB-MSC)”

Research paper thumbnail of Effects of short- and long-term swimming on myostatin expression in red and white gastrocnemius muscle and heart muscle of the rat

Research paper thumbnail of Expression of Tgf-beta in the regenerative process of crinoid echinoderms

Research paper thumbnail of Effect of MLS® laser therapy for the treatment of experimentally induced acute tendinopathy in sheep – a preliminary study

Research paper thumbnail of Contractile properties of different types of dog muscle fibres

Research paper thumbnail of Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do

Frontiers in Bioengineering and Biotechnology, 2020

Research paper thumbnail of The natural involution of the sheep proximal sesamoidean ligament is due to depletion of satellite cells and simultaneous proliferation of fibroblasts: Ultrastructural evidence

Research in Veterinary Science, 2019

Research paper thumbnail of Investigations of the corneal epithelium in Veterinary Medicine: State of the art on corneal stem cells found in different mammalian species and their putative application

Research in Veterinary Science, 2018

Research paper thumbnail of Muscle spindles of the rat sternomastoid muscle

European Journal of Translational Myology, 2018

The sternomastoid (SM) muscle in rodents presents a peculiar distribution of fiber types with a s... more The sternomastoid (SM) muscle in rodents presents a peculiar distribution of fiber types with a steep gradient from the ventral, superficial, white portion to the dorsal, deep, red region, where muscle spindles are restricted. Cross section of the medial longitudinal third of the rat SM contains around 10,000 muscle fibers with a mean diameter of 51.28±12.62 (μm +/- SD). Transverse sections stained by Succinate Dehydrogenase (SDH) reaction clearly presents two distinct regions: the dorsal deep red portion encompassing a 40% cross section area contains a high percentage of packed SDH-positive muscle fibers, and the ventral superficial region which contains mainly SDH-negative muscle fibers. Indeed, the ventral superficial region of the rat SM muscle contains mainly fast 2B muscle fibers. These acidic ATPase pH 4.3-negative and SDH-negative 2B muscle fibers are the largest of the SM muscle, while the acidic ATPase pH 4.3-positive and SDH-positive Type 1 muscle fibers are the smallest....

Research paper thumbnail of The fusion of MyoD transcription factor with the TAT peptide induces, in conditioned medium, equine stem cells towards a myogenic lineage

Annals of Anatomy - Anatomischer Anzeiger, 2016

Research paper thumbnail of CIR-Myo News: Abstracts of the 2015 Spring Padua Muscle Days

European Journal of Translational Myology, 2015

Research paper thumbnail of Might the masson trichrome stain be considered a useful method for categorizing experimental tendon lesions?

Histology and histopathology, Jan 3, 2015

Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they ... more Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subjective, and therefore a reliable grading method would be an improvement. The main purpose of this study was to assess qualitatively the histological pattern of Masson trichrome stain in healthy and injured tendons; indeed, the known "paradox" of Masson staining was used to create an evaluation for the matrix of tendons, following experimental lesions and natural repair processes. A statistically significant difference of aniline-staining between healthy and lesioned tendons was observed. Overall, we think that the Masson staining might be regarded as an informative tool in discerning the collagen spatial arrangement and therefore the histological characteristic...

Research paper thumbnail of Effect of TAT proteins on the differentiative pattern of equine mesenchymal stromal cells

Research paper thumbnail of In vitro and in vivo biocompatibility of marine-collagen biomimetic matrices: innovative applications for tendon and skin regenerative medicine

Research paper thumbnail of New regenerative strategies for tendon repair and regeneration

Annals of Anatomy - Anatomischer Anzeiger, 2014

Research paper thumbnail of 2B Myosin Heavy Chain Isoform Expression in Bovine Skeletal Muscle

Veterinary Research Communications, 2004

Research paper thumbnail of Analysis of neuromuscular junctions and effects of anabolic steroid administration in the SOD1G93A mouse model of ALS

Molecular and Cellular Neuroscience, 2012

Several lines of evidence indicate that neuromuscular junction (NMJ) destruction and disassembly ... more Several lines of evidence indicate that neuromuscular junction (NMJ) destruction and disassembly is an early phenomenon in amyotrophic lateral sclerosis (ALS). Here we analyzed by confocal and electron microscopy the NMJ structure in the diaphragm of SOD1G93A mice at symptom onset. In these mice, which provide a model for familial ALS, diaphragm denervation (~50%) as well as gastrocnemius denervation (~40%) was found. In addition, the size of the synaptic vesicle pool was reduced and alterations of mitochondria were observed in approximately 40% of the remaining presynaptic terminals. Chronic treatment of SOD1G93A mice with the anabolic steroid nandrolone during the presymptomatic stage preserved the diaphragm muscle mass and features indicative of synaptic activity. These features were represented by the number of vesicles docked within 200 nm from the presynaptic membrane and area of acetylcholine receptor clusters. Structural preservation of mitochondria was documented in presynaptic terminals. However, innervation of diaphragm muscle fibers was only slightly increased in nandrolone-treated SOD1-mutant mice. Altogether the results point out and define fine structural alterations of diaphragm NMJs in the murine model of familial ALS at symptom onset, and indicate that nandrolone may prevent or delay structural alterations in NMJ mitochondria and stimulate presynaptic activity but does not prevent muscle denervation during the disease.

Research paper thumbnail of PCB exposure and regeneration in crinoids (Echinodermata)

Marine Ecology Progress Series, 2001

Echinoderm regeneration provides a convenient and tractable test to monitor the effects of persis... more Echinoderm regeneration provides a convenient and tractable test to monitor the effects of persistent micropollutants on the developmental physiology of marine animals. Regeneration involves crucial and sensitive biological processes (cell proliferation, morphogenesis, differentiation, tissue renewal) that make it an ideal tool to assess micropollutants at the tissue and cellular level. Our results provide evidence that the regenerative response in the crinoid Antedon mediterranea is especially sensitive to PCBs and that exposure to these chemicals induces significant variations in timing and modality of arm regeneration. Prolonged exposure to low concentrations of Aroclor ® 1260 (14 ng l-1 of total PCBs), typical of moderately polluted coastal zones, results in abnormal arm growth in terms of both gross morphology and microscopic anatomy. In terms of tissue/cellular aspects the main modifications are: accelerated growth of the regenerate; massive cell migration/proliferation; hypertrophic development of the coelomic canals; and, extensive rearrangement of differentiated tissues of the stump. The anomalies observed in the developmental regenerative processes appear to be compatible with a pattern of pseudo-endocrine activities.