Marcus Oliveira - Academia.edu (original) (raw)

Papers by Marcus Oliveira

Values of bioenergetic efficiency (slope) were expressed as mean ± SD of OXPHOS versus m... more Values of bioenergetic efficiency (slope) were expressed as mean ± SD of OXPHOS versus maximum uncoupled respiratory rate linear regression and correlation analyses made in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120600#pone.0120600.s007" target="_blank">S7 Fig.</a> Correlation coefficient values (Spearman or Pearson) were depicted as superscript letters “a” or “b”, respectively. P values represent the statistical significance of linear regression slopes in each group. Bioenergetic capacity (OXPHOS) values represent the respiratory rates data induced by ADP and calculated by subtracting the ADP rates by their equivalent "leak" values shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120600#pone.0120600.t003" target="_blank">Table 3</a> (for isolated mitochondria) and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120600#pone.0120600.t004" target="_blank">Table 4</a> (for flight muscle). Statistical analyses on bioenergetic capacity in isolated mitochondria were performed by using Mann Whitney test (indicated by superscript astersiks) as well as ANOVA and a posteriori Tukey´s test (indicated by superscript symbols) for flight muscle. Significant differences in isolated mitochondria were* p<0.005 relative to G3P and PC+Mal, and** p<0.0001 relative to PC+Mal. In flight muscle, significant difference was#p<0.001 relative to G3P.Mitochondrial bioenergetic efficiency and capacity in A. aegypti females flight muscle using different substrates.</p

Oxygen consumption rates from isolated mitochondria (A-E) and whole permeabilized flight... more Oxygen consumption rates from isolated mitochondria (A-E) and whole permeabilized flight muscle (F-H) from females (solid bars) and males (hatched bars) were calculated from values shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120600#pone.0120600.g003" target="_blank">Fig. 3</a>. Data are expressed as mean ± SD of at least seven different experiments. Comparisons between groups were done by Student´s t tests. Figure (B): bp<0.001 relative to female; Figure (G): ap<0.0001 relative to female; Figure (H): bp<0.038 relative to female.</p

Heme crystallization reactions were induced in vitro mediated by uPC,... more Heme crystallization reactions were induced in vitro mediated by uPC, uPS or uPE (100 µM), a blended phospholipid mixture of commercial uPS (14%), uPC (32%) and uPE (51%) or 10 µg/mL of total lipids isolated from PMVM of R. prolixus previously fed with plasma or blood. Data are expressed as mean ± SD, of at least three different experiments and fitted using the Avrami equation as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088976#s2" target="_blank">methods section</a>. To perform the Avrami analysis, the uPC-induced kinetics were independently analyzed at early and late times, which are shown as insets.</p

(A) Total reduced thiol contents were determined by light absorption at 412 nm in female... more (A) Total reduced thiol contents were determined by light absorption at 412 nm in females (closed circles) or males (open circles) homogenates after 1h pre-incubation of intact worms with 10 μM menadione in RPMI 1640. (B) Viability of females (closed circles) or males (open circles) was determined by light microscopy after 2h pre-incubation of intact worms with different concentrations of menadione in RPMI 1640 using a previously reported motility scale [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158429#pone.0158429.ref091" target="_blank">91</a>]. Females (closed triangle) or males (open triangle) viability incubated for 2h with 50 μM menadione and 1 mM n-acetylcysteine (NAC) were also determined. Data were expressed as mean ± SEM of four (A) or three (B) different experiments. Comparisons between groups were done by Student´s t-test (A) and two-way ANOVA and a posteriori Bonferroni (B). Fig (A): * p<0.05. Fig (B): *** p<0.001.</p

The contribution of site IF, ProDH+other dehydrogenases, G3PDH+ot... more The contribution of site IF, ProDH+other dehydrogenases, G3PDH+other dehydrogenases and ETF:QOR+other dehydrogenases sites were calculated in A. aegypti mitochondria isolated from females (solid colors) and males (hatched bars) from data shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120600#pone.0120600.t008" target="_blank">Table 8</a>. Data are expressed as mean ± SD of at least five different experiments. Comparisons between groups were done by Mann Whitney test. Figure (D): ap<0.01 relative to female IF (PC+Mal).</p

Values were expressed as mean ± SEM of five distinct kinetic parameters obtained from th... more Values were expressed as mean ± SEM of five distinct kinetic parameters obtained from the data shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088976#pone-0088976-g004" target="_blank">Figure 4</a>. Statistical analyses between groups were performed by using the Mann-Whitney (superscript letters) or Student's t tests (superscript symbols). ap<0.05 relative to uPC; bp<0.01 relative to uPC; cp<0.05 relative to uPS; dp<0.005 relative to uPS; ep<0.005 relative to uPE; fp<0.001 relative to uPE; gp<0.0005 relative to RML plasma. Comparisons of reactions half-lives showed that all groups were statistically distinct among each other (p<0.03), with exception of the uPE and Blend comparison (p = 0.88).</p

O presente texto buscará discorrer sobre o início da inserção alemã no Paraná. Os conhecimentos t... more O presente texto buscará discorrer sobre o início da inserção alemã no Paraná. Os conhecimentos trazidos pelos primeiros imigrantes germânicos na primeira metade do século XIX, bem como seus posicionamentos sociais, nortearão tal propósito. Assim, a opção por um estudo de caso figura como significativamente viável. Nesse sentido, a cena imigratória em Curitiba-PR será ilustrada a seguir, enfatizando os primeiros imigrantes alemães que chegaram à capital paranaense. A chegada dos alemães em Curitiba transformou a cidade, e tal mudança não foi planejada pelo governo imperial. O pioneiro chamava-se Miguel Müller, chegando à capital paranaense em 1833

The fluorometric Amplex™ UltraRed AmR assay is frequently used for quantitative assessment of hyd... more The fluorometric Amplex™ UltraRed AmR assay is frequently used for quantitative assessment of hydrogen peroxide production. It is specific to H2O2, can be calibrated accurately, and allows continuous real-time measurement. Without correction for the background fluorescence slope, however, H2O2-independent formation of the fluorescent product UltroxRed (or resorufin from Amplex™ Red) leads to artefacts. We analysed (1) the medium specificity of the background fluorescence slope of the AmR assay, and (2) the oxygen dependence of H2O2 flux in baker´s yeast Saccharomyces cerevisiae. Apparent H2O2 flux, O2 concentration, and O2 flux were measured simultaneously by high-resolution respirometry equipped with the fluorescence module. The apparent H2O2 flux of yeast showed a maximum under hypoxia when incubated in Dulbecco´s Phosphate Buffered Saline DPBS or KCl-medium. This hypoxic peak Facts and artefacts in the AmR assay

Brazilian Journal of Radiation Sciences, 2022

The aim of this study was to evaluate the feasibility of using acrylonitrile butadiene styrene (... more The aim of this study was to evaluate the feasibility of using acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) 3D printing filaments as materials for mammography phantom construction, comparing their attenuation properties at two different set-ups: at a Calibration Laboratory and directly to a mammography unit. The attenuation of 3D printed test phantoms of two types of common 3D printing Fused Filament Fabrication (FFF) filaments (ABS and PLA) were characterized in comparison with polymethylmethacrylate (PMMA). The measurements were carried out with standard IEC 61267 X-rays, using RQR 2-M and RQR 4-M beam qualities at the Instruments Calibration Laboratory, and then applied to a mammography unit, with measurements with 28 kVp and 35 kVp. Attenuation characteristics evaluated indicates the suitable equivalence of PLA to PMMA for 3D printing breast tissue equivalent complex phantoms. The plastic materials used in this study suggest that the FFF technique may be suit...

Cells, 2021

Flight dispersal represents a key aspect of the evolutionary and ecological success of insects, a... more Flight dispersal represents a key aspect of the evolutionary and ecological success of insects, allowing escape from predators, mating, and colonization of new niches. The huge energy demand posed by flight activity is essentially met by oxidative phosphorylation (OXPHOS) in flight muscle mitochondria. In insects, mitochondrial ATP supply and oxidant production are regulated by several factors, including the energy demand exerted by changes in adenylate balance. Indeed, adenylate directly regulates OXPHOS by targeting both chemiosmotic ATP production and the activities of specific mitochondrial enzymes. In several organisms, cytochrome c oxidase (COX) is regulated at transcriptional, post-translational, and allosteric levels, impacting mitochondrial energy metabolism, and redox balance. This review will present the concepts on how COX function contributes to flying insect biology, focusing on the existing examples in the literature where its structure and activity are regulated not ...

Agrarian, 2011

Objetivou-se avaliar a produção de biomassa, o crescimento radicular e a composição bromatológica... more Objetivou-se avaliar a produção de biomassa, o crescimento radicular e a composição bromatológica das frações folha, colmo e raiz da Brachiaria brizantha cv. MG-5 Vitória, oriunda de sementes revestidas ou tratadas com inseticidas. Testou-se 4 tratamentos, sendo eles SP: sementes puras, SR: sementes revestidas, SPI: sementes puras tratadas com inseticida e SRI: sementes revestidas e tratadas com inseticida, em 4 períodos de avaliação, ou seja, P15: 0 a 15 dias, P30: 0 a 30 dias, P45: 0 a 45 dias e P60: 0 a 60 dias após a germinação das sementes. A metodologia de avaliação da produção de biomassa foi baseada em medições no início e no final de cada período, da altura da planta e no número de perfilhos, seguido por corte da planta e separação morfológicas de folha, caule e raiz. As amostras das frações separadas foram analisadas quimicamente. Em média foram observados teores de MS, PB, FDN e MM para a parte aérea (folha e colmo) de 20,56; 9,04; 66,61 e 4,32 %, respectivamente. Até os ...

Anais da Academia Brasileira de Ciências, 2021

Scientists are facing enormous pressures posed by growing scientifi c communities and stagnant/re... more Scientists are facing enormous pressures posed by growing scientifi c communities and stagnant/reduced funding. In this scenario, mechanisms of knowledge achievement and management, as well as how recruitment, progression and evaluation are carried out should be reevaluated. We argue here that knowledge has become a profi table commodity and, as a consequence, excessive academic quantifi cation, individual output assessment problems and abusive editorial market strategies have reached unsustainable levels. We propose to reinforce existing guidelines and to establish new ones to overcome these issues. Our proposal, the Initiative for Responsible Scientifi c Assessment (IRSA), has the main goal to strengthen and expand previous movements in the scientifi c community to promote higher quality research assessment, focused on better Science.

Free Radical Biology and Medicine, 2017

Journal of Geographic Information System, 2020

This work focused on the prediction modeling of Cerrado occurrences in the Atlantic Rainforest pr... more This work focused on the prediction modeling of Cerrado occurrences in the Atlantic Rainforest predominance in the São Paulo Metropolitan Region (SPMR), the fourth largest urban area, in the city of Guarulhos, southeastern Brazil. The methodology was based on the selection of records of occurrence of Cerrado in georeferenced databases, thematic maps of geoenvironmental layers, and modeling of the distribution of species through the MaxEnt tool. Besides that, field research confirmed the presence or not of the species in the areas with a high probability of occurrence of Cerrado (≥0.7). As a result, the model observed a great capacity for the prediction of the occurrence of Cerrado in Ecotonal and anthropic regions (AUC = 0.82), revealing important hotspots such as relics from the past or Cerrado enclaves of high biodiversity. The work also points priority areas for the conservation and preservation of this increasingly endangered biome.

Brazilian Journal of Radiation Sciences, 2022

Cells, 2021

Agrarian, 2011

Anais da Academia Brasileira de Ciências, 2021

Free Radical Biology and Medicine, 2017

Journal of Geographic Information System, 2020