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Papers by Maria Basciu

Research paper thumbnail of Epitope Recognition in the Human-Pig Comparison Model on Fixed and Embedded Material

Epitope Recognition in the Human-Pig Comparison Model on Fixed and Embedded Material

Journal of Histochemistry & Cytochemistry, 2015

The conditions and the specificity by which an antibody binds to its target protein in routinely ... more The conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. Direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. We aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig-the farmed mammal evolutionarily closest to humans besides apes. We used a database of 146 anti-human antibodies and found that antibodies tolerate partially conserved amino acid substitutions but not changes in target accessibility, as defined by epitope prediction algorithms. Some epitopes are sensitive to fixation and embedding in a species-specific fashion. We also find that half of the antibodies stain porcine tissue epitopes that have 60% to 100% similarity to human tissue at the amino acid sequence level. The reason why the remaining antibodies fail to stain the tissues remains elusive. Because of its similarity with the human, pig tissue offers a convenient tissue for quality control in immunohistochemistry, within and across laboratories, and an interesting model to investigate antibody specificity.

Research paper thumbnail of Phytoestrogens and breast carcinoma

Phytoestrogens and breast carcinoma

European Journal of Cancer Prevention, 2014

Research paper thumbnail of The value of repeat biopsy in the management of lupus nephritis: an international multicentre study in a large cohort of patients

Nephrology Dialysis Transplantation, 2013

Background. The International Society of Nephrology/Renal Pathology Society (ISN/RPS) classificat... more Background. The International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification represents the gold standard for the histological evaluation of Systemic Lupus Erythematosus (SLE) nephritis. A repeat biopsy (RB) might be an important tool to provide information on long-term renal outcomes and optimal therapy. Aims of this study were to evaluate the use of the ISN/RPS classification and the role of RB in routine clinical practice. Methods. A total number of 142 patients with SLE nephritis and with adequate reference and RB samples were included in this multicentre retrospective study. A meticulous histological examination was centrally performed on first and RB and compared with clinical variables and follow-up data.

Research paper thumbnail of Alveolar adenoma of the lung: unusual diagnosis of a lesion positive on PET scan. A case report

Journal of Cardiothoracic Surgery, 2012

The authors report a clinical case of alveolar adenoma presenting as a solitary pulmonary nodule ... more The authors report a clinical case of alveolar adenoma presenting as a solitary pulmonary nodule which was positive to PET and deeply located in the lung. Few cases of alveolar adenomas have been reported in literature; these lesions are considered pulmonary neoplasms with benign behaviour, usually presenting as a peripheral or subpleural coin lesion; the PET activities of such neoplasms were unknown. The present clinical case was singular for the deep location of the nodule and its tight adhesion to left inferior pulmonary vein requiring a lobectomy. In addition, alveolar adenoma PET behaviour has been reported as light positivity.

Research paper thumbnail of Epitope Recognition in the Human-Pig Comparison Model on Fixed and Embedded Material

Epitope Recognition in the Human-Pig Comparison Model on Fixed and Embedded Material

Journal of Histochemistry & Cytochemistry, 2015

The conditions and the specificity by which an antibody binds to its target protein in routinely ... more The conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. Direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. We aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig-the farmed mammal evolutionarily closest to humans besides apes. We used a database of 146 anti-human antibodies and found that antibodies tolerate partially conserved amino acid substitutions but not changes in target accessibility, as defined by epitope prediction algorithms. Some epitopes are sensitive to fixation and embedding in a species-specific fashion. We also find that half of the antibodies stain porcine tissue epitopes that have 60% to 100% similarity to human tissue at the amino acid sequence level. The reason why the remaining antibodies fail to stain the tissues remains elusive. Because of its similarity with the human, pig tissue offers a convenient tissue for quality control in immunohistochemistry, within and across laboratories, and an interesting model to investigate antibody specificity.

Research paper thumbnail of Phytoestrogens and breast carcinoma

Phytoestrogens and breast carcinoma

European Journal of Cancer Prevention, 2014

Research paper thumbnail of The value of repeat biopsy in the management of lupus nephritis: an international multicentre study in a large cohort of patients

Nephrology Dialysis Transplantation, 2013

Background. The International Society of Nephrology/Renal Pathology Society (ISN/RPS) classificat... more Background. The International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification represents the gold standard for the histological evaluation of Systemic Lupus Erythematosus (SLE) nephritis. A repeat biopsy (RB) might be an important tool to provide information on long-term renal outcomes and optimal therapy. Aims of this study were to evaluate the use of the ISN/RPS classification and the role of RB in routine clinical practice. Methods. A total number of 142 patients with SLE nephritis and with adequate reference and RB samples were included in this multicentre retrospective study. A meticulous histological examination was centrally performed on first and RB and compared with clinical variables and follow-up data.

Research paper thumbnail of Alveolar adenoma of the lung: unusual diagnosis of a lesion positive on PET scan. A case report

Journal of Cardiothoracic Surgery, 2012

The authors report a clinical case of alveolar adenoma presenting as a solitary pulmonary nodule ... more The authors report a clinical case of alveolar adenoma presenting as a solitary pulmonary nodule which was positive to PET and deeply located in the lung. Few cases of alveolar adenomas have been reported in literature; these lesions are considered pulmonary neoplasms with benign behaviour, usually presenting as a peripheral or subpleural coin lesion; the PET activities of such neoplasms were unknown. The present clinical case was singular for the deep location of the nodule and its tight adhesion to left inferior pulmonary vein requiring a lobectomy. In addition, alveolar adenoma PET behaviour has been reported as light positivity.

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