Maria Dencheva - Academia.edu (original) (raw)

Papers by Maria Dencheva

Updates in Hemodialysis, 2015

Medicina Oral Patología Oral y Cirugia Bucal, 2012

Medicina Oral Patología Oral y Cirugia Bucal, 2012

Dokladi na Bʺlgarskata akademiâ na naukite, Apr 26, 2024

In the present study we performed a comparative in vitro evaluation of biocompatibility of two ni... more In the present study we performed a comparative in vitro evaluation of biocompatibility of two nickel-containing dental alloys produced by two different methods-conventional and powder metallurgy. Stem cells from apical papilla (SCAPs) were isolated, cultured and incubated in 6% solution of alloys powders in DMSO for 48 hours and further examined for apoptosis, proliferation and cellular metabolism. Our findings suggest that the alloys produced by the traditional method (Crystalloy N) release more electrically charged products of electrochemical corrosion, resulting in increased ROS production in SCAPs, increased percentage of apoptoic cells, decreased proliferative potential and tend to increase antioxidant enzymatic defence. No allergic pro-inflammatory effect of the metal alloys on stem cell cultures was confirmed.

Acta Scientific Medical Sciences, 2020

We present a case of an intradermal allergy test involving positive and negative reactions to all... more We present a case of an intradermal allergy test involving positive and negative reactions to allergens. The standard reading is supplemented by thermal imaging and calculation of an indicator related to the change in temperature due to allergic reactions in the examined skin areas (dT). The results show that the temperature reading can complement the standard test, and the dT indicator can be used to distinguish positive from negative reactions.

Applications of Calorimetry, 2022

Calorimetry is an indisputable diagnostic method. Over the years, there has been an improvement i... more Calorimetry is an indisputable diagnostic method. Over the years, there has been an improvement in the equipment and methods for measuring the calor that accompanies various processes. Using a thermal camera, we can measure the surface temperature of the skin at the beginning and the end of each skin allergy test. They are epicutaneous, cutaneous, and percutaneous. In case of a positive reaction, allergic inflammation is observed with the obligatory symptoms, one of which is warming (calor). Measuring and visualizing this warming is essential in the diagnosis of allergic reaction. The methodology of imaging the skin areas and processing the results is the key point in the objectivity of the study. Diagnostic skin allergy tests report mainly immunopathological reactions of the first and fourth types (Coombs and Gel classification). Their course is different and this necessitated the development of various thermovisiographic imaging methods. Through the results of our thermal imaging ...

International Journal of Medical Reviews and Case Reports, 2020

Summary: A lot of latex products are used every day in medical and dental practice, as well as in... more Summary: A lot of latex products are used every day in medical and dental practice, as well as in households. This could lead to the development of sensibilisation amongst work-related groups of people, as well as in patients. Reactions that could be seen are not only affecting the skin, presented mainly like allergic contact dermatitis, but might be also severe life-threatening ones. The aim is to present a case of allergic reaction tolatex during regular skin prick testing. Clinical case: We present a clinical case of 47-year-old woman who has been forwarded to the Department of “Imaging and Oral Diagnostics”, Faculty of dental medicine, Medical University-Sofia, for testing the sensitization to latex products because of history for burning sensation, throbbing and itching in the area of the hard palate, lips and the pick of the tongue after regular dental clinical examination with latex gloves. Results: Three different types of testing were conducted for proving a latex allergy- serological examination: ImmunoCAP Phadia, prick-test and epicutaneous test. Additional testings were performed for clarifying the general allergic status of the patient. Conclusion: The diagnosing of sensitization to latex proteins seems to be easily doable but in fact it turns out to be challenging in clinical settings. Despite the performed three diagnostic tests (which are with different results), of great importance turns out to be the prick-testing and the initial clinical symptoms of allergic reaction which is manifested with feeling for edema and itching in the oral cavity. The administration of adrenaline, corticosteroids and antihistamines, included in the protocol for management of emergency situations in this specific case prevented the development of more severe condition.

Journal of IMAB - Annual Proceeding (Scientific Papers), 2011

The aim of this study was to determine the tonguecoating status (using modern index system), the ... more The aim of this study was to determine the tonguecoating status (using modern index system), the degree of gingival hyperplasia, to register the observed oral lesions in 30 patients with replaced renal function (hemodialysis and renal transplanted).

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

Introduction: The application of thermovision in endodontics is an innovative method and has a gr... more Introduction: The application of thermovision in endodontics is an innovative method and has a great informative value, evidence proved, thanks to the use of last generation camera Flir A310 and a software ThermaCam Reporter 8.3 Pro. In the recent years a new method of root canal disinfection is introduced-photo-activated disinfection (PAD). Temperature increase have always been a concern during dental laser treatment. Aim: The purpose of our study is to evaluate the temperature elevation on the outer root surface at extracted teeth and in periapical tissues when photodynamic therapy with 630-nm diode laser is used for root canal disinfection. Materials and methods: For the laboratory study we used 72 single-rooted freshly extracted human teeth. They were divided into six groups depending on the power of the light source (220 mW, 360 mW and 500 mW) and the type of photosensitizer agent-Fotosan and Zn-phtalocianine. In our clinical study participated 8 patients, who came in to our clinic with complaints of spontaneous pain or were with infected root canals and were referred for endodontic treatment. PAD was performed with a diode laser with wavelength of 630 nm using an endodontic handpiece with fiberoptic connector, allowing penetration to 4 mm from the apical stop, and movements of fibreoptic (200 µm) in apicalcoronal direction. Results: The highest temperature value was detected at 500mW and 360mW. The average temperature increase recorded in our study at the external surface of the root canal did not exceed 10°C only at power setting of 220 mW during PAD with Zn-phtalocianine. Conclusion: In conclusion, photo-activated disinfection of the root canal with Zn-phtalocianine and 630-nm diode laser using 200 µm fibreoptic tips at a power setting of 220 mW for 60 seconds induces a temperature increase which isn't sufficient to damage the neighbouring tissues. Based on this results we don't recommend root canal preparation over 02 or 04 taper when PAD will be performed.

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

In dental practice is common concomitant exposure to formaldehyde andglutaraldehyde. Formaldehyde... more In dental practice is common concomitant exposure to formaldehyde andglutaraldehyde. Formaldehyde is an ingredient of some dental materials and may bereleased from methacrylate-based dental materials. A multitude of methacrylicmonomers is used in dentistry.In the present study we evaluated the prevalence ofmultiple contact hypersensitivity to dental allergens (methacrylic monomers, mercuryand eugenol) among dental professionals, students of dental medicine and patients,allergic to formaldehyde and/or glutaraldehyde. Methods: A total of 139 participants were included in the study: occupationally exposed to dentalprofessionals, students of dental medicine and patients without occupational exposure.All of them were patch-tested with formaldehyde, glutaraldehyde, some methacrylicmonomers, mercury and eugenol. The results were subject to statistical analysis (p <0.05) Results: High incidence of contact allergy to formaldehyde and/or glutaraldehyde was revealed.The most vulnerable group in terms of concomitant sensitization to formaldehydeand/orglutaraldehyde and to MMA was the one of students from 6th course; toTREGDMA and EGDMA-students from 3-4 courses; to 2-HEMA and mercury-dentalprofessionals; to tetrahydrofurfuryl methacrylate and to eugenol-students from 3-4courses and dental professionals. High rates of concomitant sensitization toformaldehyde and/or glutaraldehyde and to the tested dental materials wereestablished both in occupational and nonoccupational exposure, suggesting animportant role of consumer (patient's) exposure as well. Conclusions; More effective training and risk management programs in exposure to allergens anduse of proper personal protection in dental practice and among dental students couldbe recommended.

Biotechnology & Biotechnological Equipment, 2010

The purpose of our research is to estimate the motivation for oral health and whether patients ar... more The purpose of our research is to estimate the motivation for oral health and whether patients are well informed about fields of disturbance in the maxillofacial area. A total of 170 subjects participated in the research: 59 (34.7%)-on hemodialysis; 51 (30%) with renal transplant; and 60% (35.3%) as a control group. Based upon the direct individual questionnaire, it became clear that there was a lack of bilateral motivation for support of oral health and insufficient knowledge of the patients regarding oral health prophylactics, as well as lack of understanding of dental focal problematic. The attitude of medics and dentists towards the dental sanation of this population of patients was not on sufficiently high level either. In order to respond completely to the health needs of patients that are on hemodialysis and after renal transplantation, it is necessary that dental studies in Bulgaria are introduced within the sphere of transplantation medicine.

Hemodialysis - Different Aspects, 2011

Folia Medica, Mar 31, 2020

Background: Dental students are at high risk of developing adverse respiratory reactions as a res... more Background: Dental students are at high risk of developing adverse respiratory reactions as a result of their practical work during their dentistry degree program when they come into contact with multiple airborne irritants and allergens. Aim: To estimate the prevalence of respiratory symptoms of exposure to substances in the workplace and associated risk factors in Bulgarian dental students.

The compex dental focal sanitation is a novel method in dental medicine, which entails specific d... more The compex dental focal sanitation is a novel method in dental medicine, which entails specific dental diagnostic and treatment (2). The relationship between focal doctrine from dental origin and systemic disease directs our attention to some unexpected causes. The aim of this study is to observed the opinion of patients about focal dental diagnostic and treatment in order to better organize and improve dental care. 88 patients compromised patients (43 on hemodialysis and 45 with renal transplants) completed tailored survey. Key words: hemodialysis, renal transplantation, dental focus.

Biotechnology & Biotechnological Equipment

The purpose of this study was to evaluate the incidence of contact sensitization to metals used i... more The purpose of this study was to evaluate the incidence of contact sensitization to metals used in dental practice among students of dental medicine, students from a dental technician school, dental professionals and patients. A total of 128 participants: 28 dental professionals, 40 students of dental medicine, 38 students from a dental technician school and 22 patients without occupational exposure to metals, were patch tested with potassium dichromate, cobalt(II) chloride hexahydrate, copper(II)sulfate pentahydrate, palladium(II)chloride, aluminium(III)chloride hexahydrate, gold(I)sodium thiosulfate dehydrate, tin and nickel(II)sulfate hexahydrate. The main metal allergens for students of dental medicine were cobalt(II)chloride hexahydrate, gold(I)sodium thiosulfate dehydrate, nickel(II)sulfate hexahydrate and potassium dichromate; for students from the dental technician schoolcobalt(II)chloride hexahydrate, nickel(II)sulfate hexahydrate and gold (I)sodium thiosulfate dehydrate; for dental professionalscobalt(II)chloride hexahydrate, palladium (II)chloride and nickel(II)sulfate hexahydrate, and for dental patientscobalt(II)chloride hexahydrate, nickel(II)sulfate hexahydrate and copper(II)sulfate pentahydrate. There was a significantly higher incidence of sensitization to potassium dichromate (x 2 = 10.497, p = 0.012), palladium(II)chloride (x 2 = 5.381, p = 0.02) and gold(I)sodium thiosulfate dehydrate (x 2 = 6.347, p = 0.018) in the group of students of dental medicine. Our findings confirm the importance of cobalt (II)chloride hexahydrate as contact sensitizer for all the studied groups. Students of dental medicine could be defined as a group of particular risk of contact sensitization with metals. We recommend that knowledge on the sensitizing properties of metals be provided at the very beginning of the course of practical education in dentistry, together with application of adequate preventive measures.

Journal of IMAB - Annual Proceeding (Scientific Papers)

Sensitization to metals is a significant problem for both dental patients treated with dental mat... more Sensitization to metals is a significant problem for both dental patients treated with dental materials and for dental professionals in occupational exposures. The purpose of the present study was to evaluate the incidence of concurrent contact sensitization to relevant for dental practice metals among students of dental medicine, students from dental technician school, dental professionals and patients. Material and Methods: A total of 128 participants were included in the study. All of them were patch-tested with nickel, cobalt, copper, potassium dichromate, palladium, aluminium, gold and tin. The results were subject to statistical analysis (p < 0.05). Results: For the whole studied population, potassium dichromate exhibited concomitant reactivity most often; copper and tin also often manifested co-reactivity. For the groups, exposed in dental practice, potassium dichromate and tin were outlined as the most often co-reacting metal allergens, but statistical significance concerning the co-sensitization to copper and the other metals was established only for aluminium. An increased incidence and OR for concomitant sensitization to cobalt and nickel was established in the group of dental students; to copper and nickel-in the control group; to palladium and nickel-in the group of dental professionals, the group of students of dental medicine and in the control group; to potassium dichromate and cobalt-in the group of dental students; to copper and palladium-in the control group of dental patients; to potassium dichromate and copper-in the group of dental professionals; to copper and aluminum-in the groups of students from dental technician school and of dental professionals; to copper and gold-in the groups of dental professionals and in the group of dental patients; to potassium dichromate and aluminum-in the group of dental professionals; to potassium dichromate and goldin the group of dental professionals, and to aluminium and tin-in the groups of dental professionals and in the group of dental patients. Conclusions: Our data indicate a high risk of concurrent contact sensitization to the studied relevant for dental practice metals. Since the role of occupational exposures can't be excluded, adequate preventive measures and information should be provided. General population also need to be well informed and the European Union regulations to be properly applied.

Journal of Thermal Analysis and Calorimetry

In allergology, skin diagnostic tests are divided into epicutaneous, cutaneous and intracutaneous... more In allergology, skin diagnostic tests are divided into epicutaneous, cutaneous and intracutaneous. The prick test is one of the cutaneous tests used most often for the diagnosis of hypersensitivity to local anesthetics. In the case of hypersensitivity to the examined anesthetic and reaction to the histamine-containing solution (positive control), inflammation appears with an increase in the local temperature. This warming can be detected objectively with a thermal camera and can be used in the diagnostic process. This study was used to examine the capabilities of the infrared thermography application, in the prick test of both men and women, by comparing temperature changes and the intensity of allergic inflammation in the positive and negative controls in addition with a negative reaction to an allergen. A group of 115 patients-55 male (52%) and 60 female (48%), are included. All are examined for hypersensitivity to Mepivastesin, together with the positive and negative control. Skin temperature changes of the skin were examined with FLIR A320 thermal camera and the FLIR Reporter Professional software 2013-application used to process the thermal camera images and data. The statistical analysis shows a marked temperature difference between men and women. Similarities in temperature values are detected between the negative reactions to dental anesthetic and the negative control. However, the latter substantially differs from the data obtained with the positive control. Results of the research indicate the possible applications of the thermography diagnostics in evaluation of the prick test results. Keywords Immediate hypersensitivity Á Skin test Á Infrared thermography Á Skin temperature List of symbols DAr The change in temperature of the skin after performing the test with the allergen Ar 2 The post-reaction temperature of the skin after performing the test with the allergen Ar 1 The pre-reaction temperature of the skin after performing the test with the allergen DNeg The change in the temperature of the skin after performing the test with the negative control Neg 2 The post-reaction temperature of the skin after performing the test with the negative control Neg 1 The pre-reaction temperature of the skin after performing the test with the negative control DPos The change in temperature of the skin after performing the test with the positive control Pos 2 The post-reaction temperature of the skin after performing the test with the positive control Pos 1 The pre-reaction temperature of the skin after performing the test with the positive control DAr 0 The change of the temperature due to the allergic reaction

Updates in Hemodialysis, 2015

Medicina Oral Patología Oral y Cirugia Bucal, 2012

Medicina Oral Patología Oral y Cirugia Bucal, 2012

Dokladi na Bʺlgarskata akademiâ na naukite, Apr 26, 2024

In the present study we performed a comparative in vitro evaluation of biocompatibility of two ni... more In the present study we performed a comparative in vitro evaluation of biocompatibility of two nickel-containing dental alloys produced by two different methods-conventional and powder metallurgy. Stem cells from apical papilla (SCAPs) were isolated, cultured and incubated in 6% solution of alloys powders in DMSO for 48 hours and further examined for apoptosis, proliferation and cellular metabolism. Our findings suggest that the alloys produced by the traditional method (Crystalloy N) release more electrically charged products of electrochemical corrosion, resulting in increased ROS production in SCAPs, increased percentage of apoptoic cells, decreased proliferative potential and tend to increase antioxidant enzymatic defence. No allergic pro-inflammatory effect of the metal alloys on stem cell cultures was confirmed.

Acta Scientific Medical Sciences, 2020

We present a case of an intradermal allergy test involving positive and negative reactions to all... more We present a case of an intradermal allergy test involving positive and negative reactions to allergens. The standard reading is supplemented by thermal imaging and calculation of an indicator related to the change in temperature due to allergic reactions in the examined skin areas (dT). The results show that the temperature reading can complement the standard test, and the dT indicator can be used to distinguish positive from negative reactions.

Applications of Calorimetry, 2022

Calorimetry is an indisputable diagnostic method. Over the years, there has been an improvement i... more Calorimetry is an indisputable diagnostic method. Over the years, there has been an improvement in the equipment and methods for measuring the calor that accompanies various processes. Using a thermal camera, we can measure the surface temperature of the skin at the beginning and the end of each skin allergy test. They are epicutaneous, cutaneous, and percutaneous. In case of a positive reaction, allergic inflammation is observed with the obligatory symptoms, one of which is warming (calor). Measuring and visualizing this warming is essential in the diagnosis of allergic reaction. The methodology of imaging the skin areas and processing the results is the key point in the objectivity of the study. Diagnostic skin allergy tests report mainly immunopathological reactions of the first and fourth types (Coombs and Gel classification). Their course is different and this necessitated the development of various thermovisiographic imaging methods. Through the results of our thermal imaging ...

International Journal of Medical Reviews and Case Reports, 2020

Summary: A lot of latex products are used every day in medical and dental practice, as well as in... more Summary: A lot of latex products are used every day in medical and dental practice, as well as in households. This could lead to the development of sensibilisation amongst work-related groups of people, as well as in patients. Reactions that could be seen are not only affecting the skin, presented mainly like allergic contact dermatitis, but might be also severe life-threatening ones. The aim is to present a case of allergic reaction tolatex during regular skin prick testing. Clinical case: We present a clinical case of 47-year-old woman who has been forwarded to the Department of “Imaging and Oral Diagnostics”, Faculty of dental medicine, Medical University-Sofia, for testing the sensitization to latex products because of history for burning sensation, throbbing and itching in the area of the hard palate, lips and the pick of the tongue after regular dental clinical examination with latex gloves. Results: Three different types of testing were conducted for proving a latex allergy- serological examination: ImmunoCAP Phadia, prick-test and epicutaneous test. Additional testings were performed for clarifying the general allergic status of the patient. Conclusion: The diagnosing of sensitization to latex proteins seems to be easily doable but in fact it turns out to be challenging in clinical settings. Despite the performed three diagnostic tests (which are with different results), of great importance turns out to be the prick-testing and the initial clinical symptoms of allergic reaction which is manifested with feeling for edema and itching in the oral cavity. The administration of adrenaline, corticosteroids and antihistamines, included in the protocol for management of emergency situations in this specific case prevented the development of more severe condition.

Journal of IMAB - Annual Proceeding (Scientific Papers), 2011

The aim of this study was to determine the tonguecoating status (using modern index system), the ... more The aim of this study was to determine the tonguecoating status (using modern index system), the degree of gingival hyperplasia, to register the observed oral lesions in 30 patients with replaced renal function (hemodialysis and renal transplanted).

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

Introduction: The application of thermovision in endodontics is an innovative method and has a gr... more Introduction: The application of thermovision in endodontics is an innovative method and has a great informative value, evidence proved, thanks to the use of last generation camera Flir A310 and a software ThermaCam Reporter 8.3 Pro. In the recent years a new method of root canal disinfection is introduced-photo-activated disinfection (PAD). Temperature increase have always been a concern during dental laser treatment. Aim: The purpose of our study is to evaluate the temperature elevation on the outer root surface at extracted teeth and in periapical tissues when photodynamic therapy with 630-nm diode laser is used for root canal disinfection. Materials and methods: For the laboratory study we used 72 single-rooted freshly extracted human teeth. They were divided into six groups depending on the power of the light source (220 mW, 360 mW and 500 mW) and the type of photosensitizer agent-Fotosan and Zn-phtalocianine. In our clinical study participated 8 patients, who came in to our clinic with complaints of spontaneous pain or were with infected root canals and were referred for endodontic treatment. PAD was performed with a diode laser with wavelength of 630 nm using an endodontic handpiece with fiberoptic connector, allowing penetration to 4 mm from the apical stop, and movements of fibreoptic (200 µm) in apicalcoronal direction. Results: The highest temperature value was detected at 500mW and 360mW. The average temperature increase recorded in our study at the external surface of the root canal did not exceed 10°C only at power setting of 220 mW during PAD with Zn-phtalocianine. Conclusion: In conclusion, photo-activated disinfection of the root canal with Zn-phtalocianine and 630-nm diode laser using 200 µm fibreoptic tips at a power setting of 220 mW for 60 seconds induces a temperature increase which isn't sufficient to damage the neighbouring tissues. Based on this results we don't recommend root canal preparation over 02 or 04 taper when PAD will be performed.

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

Journal of IMAB - Annual Proceeding (Scientific Papers), 2013

In dental practice is common concomitant exposure to formaldehyde andglutaraldehyde. Formaldehyde... more In dental practice is common concomitant exposure to formaldehyde andglutaraldehyde. Formaldehyde is an ingredient of some dental materials and may bereleased from methacrylate-based dental materials. A multitude of methacrylicmonomers is used in dentistry.In the present study we evaluated the prevalence ofmultiple contact hypersensitivity to dental allergens (methacrylic monomers, mercuryand eugenol) among dental professionals, students of dental medicine and patients,allergic to formaldehyde and/or glutaraldehyde. Methods: A total of 139 participants were included in the study: occupationally exposed to dentalprofessionals, students of dental medicine and patients without occupational exposure.All of them were patch-tested with formaldehyde, glutaraldehyde, some methacrylicmonomers, mercury and eugenol. The results were subject to statistical analysis (p <0.05) Results: High incidence of contact allergy to formaldehyde and/or glutaraldehyde was revealed.The most vulnerable group in terms of concomitant sensitization to formaldehydeand/orglutaraldehyde and to MMA was the one of students from 6th course; toTREGDMA and EGDMA-students from 3-4 courses; to 2-HEMA and mercury-dentalprofessionals; to tetrahydrofurfuryl methacrylate and to eugenol-students from 3-4courses and dental professionals. High rates of concomitant sensitization toformaldehyde and/or glutaraldehyde and to the tested dental materials wereestablished both in occupational and nonoccupational exposure, suggesting animportant role of consumer (patient's) exposure as well. Conclusions; More effective training and risk management programs in exposure to allergens anduse of proper personal protection in dental practice and among dental students couldbe recommended.

Biotechnology & Biotechnological Equipment, 2010

The purpose of our research is to estimate the motivation for oral health and whether patients ar... more The purpose of our research is to estimate the motivation for oral health and whether patients are well informed about fields of disturbance in the maxillofacial area. A total of 170 subjects participated in the research: 59 (34.7%)-on hemodialysis; 51 (30%) with renal transplant; and 60% (35.3%) as a control group. Based upon the direct individual questionnaire, it became clear that there was a lack of bilateral motivation for support of oral health and insufficient knowledge of the patients regarding oral health prophylactics, as well as lack of understanding of dental focal problematic. The attitude of medics and dentists towards the dental sanation of this population of patients was not on sufficiently high level either. In order to respond completely to the health needs of patients that are on hemodialysis and after renal transplantation, it is necessary that dental studies in Bulgaria are introduced within the sphere of transplantation medicine.

Hemodialysis - Different Aspects, 2011

Folia Medica, Mar 31, 2020

Background: Dental students are at high risk of developing adverse respiratory reactions as a res... more Background: Dental students are at high risk of developing adverse respiratory reactions as a result of their practical work during their dentistry degree program when they come into contact with multiple airborne irritants and allergens. Aim: To estimate the prevalence of respiratory symptoms of exposure to substances in the workplace and associated risk factors in Bulgarian dental students.

The compex dental focal sanitation is a novel method in dental medicine, which entails specific d... more The compex dental focal sanitation is a novel method in dental medicine, which entails specific dental diagnostic and treatment (2). The relationship between focal doctrine from dental origin and systemic disease directs our attention to some unexpected causes. The aim of this study is to observed the opinion of patients about focal dental diagnostic and treatment in order to better organize and improve dental care. 88 patients compromised patients (43 on hemodialysis and 45 with renal transplants) completed tailored survey. Key words: hemodialysis, renal transplantation, dental focus.

Biotechnology & Biotechnological Equipment

The purpose of this study was to evaluate the incidence of contact sensitization to metals used i... more The purpose of this study was to evaluate the incidence of contact sensitization to metals used in dental practice among students of dental medicine, students from a dental technician school, dental professionals and patients. A total of 128 participants: 28 dental professionals, 40 students of dental medicine, 38 students from a dental technician school and 22 patients without occupational exposure to metals, were patch tested with potassium dichromate, cobalt(II) chloride hexahydrate, copper(II)sulfate pentahydrate, palladium(II)chloride, aluminium(III)chloride hexahydrate, gold(I)sodium thiosulfate dehydrate, tin and nickel(II)sulfate hexahydrate. The main metal allergens for students of dental medicine were cobalt(II)chloride hexahydrate, gold(I)sodium thiosulfate dehydrate, nickel(II)sulfate hexahydrate and potassium dichromate; for students from the dental technician schoolcobalt(II)chloride hexahydrate, nickel(II)sulfate hexahydrate and gold (I)sodium thiosulfate dehydrate; for dental professionalscobalt(II)chloride hexahydrate, palladium (II)chloride and nickel(II)sulfate hexahydrate, and for dental patientscobalt(II)chloride hexahydrate, nickel(II)sulfate hexahydrate and copper(II)sulfate pentahydrate. There was a significantly higher incidence of sensitization to potassium dichromate (x 2 = 10.497, p = 0.012), palladium(II)chloride (x 2 = 5.381, p = 0.02) and gold(I)sodium thiosulfate dehydrate (x 2 = 6.347, p = 0.018) in the group of students of dental medicine. Our findings confirm the importance of cobalt (II)chloride hexahydrate as contact sensitizer for all the studied groups. Students of dental medicine could be defined as a group of particular risk of contact sensitization with metals. We recommend that knowledge on the sensitizing properties of metals be provided at the very beginning of the course of practical education in dentistry, together with application of adequate preventive measures.

Journal of IMAB - Annual Proceeding (Scientific Papers)

Sensitization to metals is a significant problem for both dental patients treated with dental mat... more Sensitization to metals is a significant problem for both dental patients treated with dental materials and for dental professionals in occupational exposures. The purpose of the present study was to evaluate the incidence of concurrent contact sensitization to relevant for dental practice metals among students of dental medicine, students from dental technician school, dental professionals and patients. Material and Methods: A total of 128 participants were included in the study. All of them were patch-tested with nickel, cobalt, copper, potassium dichromate, palladium, aluminium, gold and tin. The results were subject to statistical analysis (p < 0.05). Results: For the whole studied population, potassium dichromate exhibited concomitant reactivity most often; copper and tin also often manifested co-reactivity. For the groups, exposed in dental practice, potassium dichromate and tin were outlined as the most often co-reacting metal allergens, but statistical significance concerning the co-sensitization to copper and the other metals was established only for aluminium. An increased incidence and OR for concomitant sensitization to cobalt and nickel was established in the group of dental students; to copper and nickel-in the control group; to palladium and nickel-in the group of dental professionals, the group of students of dental medicine and in the control group; to potassium dichromate and cobalt-in the group of dental students; to copper and palladium-in the control group of dental patients; to potassium dichromate and copper-in the group of dental professionals; to copper and aluminum-in the groups of students from dental technician school and of dental professionals; to copper and gold-in the groups of dental professionals and in the group of dental patients; to potassium dichromate and aluminum-in the group of dental professionals; to potassium dichromate and goldin the group of dental professionals, and to aluminium and tin-in the groups of dental professionals and in the group of dental patients. Conclusions: Our data indicate a high risk of concurrent contact sensitization to the studied relevant for dental practice metals. Since the role of occupational exposures can't be excluded, adequate preventive measures and information should be provided. General population also need to be well informed and the European Union regulations to be properly applied.

Journal of Thermal Analysis and Calorimetry

In allergology, skin diagnostic tests are divided into epicutaneous, cutaneous and intracutaneous... more In allergology, skin diagnostic tests are divided into epicutaneous, cutaneous and intracutaneous. The prick test is one of the cutaneous tests used most often for the diagnosis of hypersensitivity to local anesthetics. In the case of hypersensitivity to the examined anesthetic and reaction to the histamine-containing solution (positive control), inflammation appears with an increase in the local temperature. This warming can be detected objectively with a thermal camera and can be used in the diagnostic process. This study was used to examine the capabilities of the infrared thermography application, in the prick test of both men and women, by comparing temperature changes and the intensity of allergic inflammation in the positive and negative controls in addition with a negative reaction to an allergen. A group of 115 patients-55 male (52%) and 60 female (48%), are included. All are examined for hypersensitivity to Mepivastesin, together with the positive and negative control. Skin temperature changes of the skin were examined with FLIR A320 thermal camera and the FLIR Reporter Professional software 2013-application used to process the thermal camera images and data. The statistical analysis shows a marked temperature difference between men and women. Similarities in temperature values are detected between the negative reactions to dental anesthetic and the negative control. However, the latter substantially differs from the data obtained with the positive control. Results of the research indicate the possible applications of the thermography diagnostics in evaluation of the prick test results. Keywords Immediate hypersensitivity Á Skin test Á Infrared thermography Á Skin temperature List of symbols DAr The change in temperature of the skin after performing the test with the allergen Ar 2 The post-reaction temperature of the skin after performing the test with the allergen Ar 1 The pre-reaction temperature of the skin after performing the test with the allergen DNeg The change in the temperature of the skin after performing the test with the negative control Neg 2 The post-reaction temperature of the skin after performing the test with the negative control Neg 1 The pre-reaction temperature of the skin after performing the test with the negative control DPos The change in temperature of the skin after performing the test with the positive control Pos 2 The post-reaction temperature of the skin after performing the test with the positive control Pos 1 The pre-reaction temperature of the skin after performing the test with the positive control DAr 0 The change of the temperature due to the allergic reaction