Maria Jiménez - Academia.edu (original) (raw)

Papers by Maria Jiménez

Research paper thumbnail of Electric permittivity and dynamic mobility of dilute suspensions of plate-like gibbsite particles

Langmuir : the ACS journal of surfaces and colloids, Jan 2, 2015

In this work we discuss the electrokinetic evaluation of model plate-like particles. By model par... more In this work we discuss the electrokinetic evaluation of model plate-like particles. By model particles we mean homogeneous and controlled size and shape. The electrokinetic analysis in such complex geometries cannot be limited to a single data point as in usual electrophoresis in constant (dc) fields. The information can be made much richer if alternating (ac) fields with a sufficiently wide range of frequencies are used. In this case, two techniques can be applied: one is the determination of the frequency spectrum of the electric permittivity or dielectric constant (low-frequency dielectric dispersion), and the other is electroacoustics of suspensions and determination of the frequency dispersion of the electrophoretic mobility (dynamic or ac mobility). In this work, these techniques are used with planar gibbsite (γ-Al(OH)3) particles, modeled as oblate spheroids with a small aspect ratio. As in other laminar minerals, a particular charge distribution, differing between edges and...

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Research paper thumbnail of Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation

Molecular and cellular biology, 1999

Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene... more Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells u...

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Research paper thumbnail of Structure-Function Dissection of Myxococcus xanthus CarD N-Terminal Domain, a Defining Member of the CarD_CdnL_TRCF Family of RNA Polymerase Interacting Proteins

PLOS ONE, 2015

Two prototypes of the large CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-binding prot... more Two prototypes of the large CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-binding proteins, Myxococcus xanthus CarD and CdnL, have distinct functions whose molecular basis remain elusive. CarD, a global regulator linked to the action of several extracytoplasmic function (ECF) σ-factors, binds to the RNAP β subunit (RNAP-β) and to protein CarG via an N-terminal domain, CarDNt, and to DNA via an intrinsically unfolded C-terminal domain resembling eukaryotic high-mobility-group A (HMGA) proteins. CdnL, a CarDNt-like protein that is essential for cell viability, is implicated in σA-dependent rRNA promoter activation and interacts with RNAP-β but not with CarG. While the HMGA-like domain of CarD by itself is inactive, we find that CarDNt has low but observable ability to activate ECF σ-dependent promoters in vivo, indicating that the C-terminal DNA-binding domain is required to maximize activity. Our structure-function dissection of CarDNt reveals an N-terminal, five-stranded β -sheet Tudor-like domain, CarD1-72, whose structure and contacts with RNAP-β mimic those of CdnL. Intriguingly, and in marked contrast to CdnL, CarD mutations that disrupt its interaction with RNAP-β did not annul activity. Our data suggest that the CarDNt C-terminal segment, CarD61-179, may be structurally distinct from its CdnL counterpart, and that it houses at least two distinct and crucial function determinants: (a) CarG-binding, which is specific to CarD; and (b) a basic residue stretch, which is also conserved and functionally required in CdnL. This study highlights the evolution of shared and divergent interactions in similar protein modules that enable the distinct activities of two related members of a functionally important and widespread bacterial protein family.

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Research paper thumbnail of Participation of N-methyl-d-aspartate receptors on methylmercury-induced DNA damage in rat frontal cortex

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Research paper thumbnail of Patología cerebro-vascular hemorrágica en adultos asociada al consumo de cocaína

Cuadernos De Medicina Forense, 2005

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Research paper thumbnail of Transitory T-Lymphoblastic Leukemoid Reaction in a Neonate with Down Syndrome

Journal of Pediatric Hematology Oncology, 1990

A transient leukemoid reaction in a neonate with Down syndrome is reported. The blastic prolifera... more A transient leukemoid reaction in a neonate with Down syndrome is reported. The blastic proliferation was identified as T lymphoblastic in an early stage of maturation (prethymocytes) using morphological, cytochemical, and immunological methods. A spontaneous complete remission occurred in 8 weeks. No additional cytogenetic alterations were found, except for those concerning chromosome 21. Other cases reported in the literature reveal that cytogenetic studies may be useful to distinguish these transient leukemic reactions from true leukemias in newborns with Down syndrome. The in vitro growth pattern of peripheral blood and bone marrow may also be useful for this purpose.

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Research paper thumbnail of Studies on age determination and growth pattern of the wedge sole Dicologoglossa cuneata (Moreau, 1881) in the Spanish waters of the Gulf of Cadiz (southwest Iberian Peninsula)

The wedge sole Dicologoglossa cuneata (Moreau, 1881) is a typical coastal soleid which is con- si... more The wedge sole Dicologoglossa cuneata (Moreau, 1881) is a typical coastal soleid which is con- sidered as one of the main target species in the demersal fishery of Spain's southern Atlantic region. This paper presents a study on the growth pattern of wedge sole in the Gulf of Cadiz. Age was determined by observing and analysing whole otoliths (sagittae), thereby

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Research paper thumbnail of P-322 Treatment of chronic hepatitis C with interferon beta

International Hepatology Communications, 1995

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Research paper thumbnail of Monitoring the process to obtain red wine enriched in resveratrol and piceatannol without quality loss

Food Chemistry, 2010

Stilbene-enriched wine is considered an interesting new food product with added value as a conseq... more Stilbene-enriched wine is considered an interesting new food product with added value as a consequence of the numerous health-promoting properties ascribed to it, mainly from its trans-resveratrol content. Postharvest grapes have been treated with ultraviolet-C light to produce stilbene-enriched grapes that were later used in a conventional winemaking process to obtain a red wine enriched in stilbenes.By measuring oenological parameters

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Research paper thumbnail of A EUROPEAN NUCLEAR DATA PROGRAMME FOR ACCELERATOR-DRIVEN SYSTEMS

In the HINDAS programme, nuclear data in the 20-2 000 MeV range are evaluated by means of a combi... more In the HINDAS programme, nuclear data in the 20-2 000 MeV range are evaluated by means of a combination of nuclear models and well-selected intermediate- and high-energy experiments. A panoply of European accelerators is utilised to provide complete sets of experimental data for iron, lead and uranium over a large energy range. Nuclear model codes are being improved and validated

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Research paper thumbnail of Chemical characterization of the lectin from Amaranthus leucocarpus syn. hypocondriacus by 2-D proteome analysis

Glycoconjugate Journal, 2001

In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amara... more In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45% of \bibeta-sheet and 5% of \bialpha-helix. Amino acid sequence of the purified lectin and its isoforms was determined from peptides obtained after trypsin digestion by MALDI-TOF (Matrix assisted laser desorption ionization-time of flight). The tryptic peptides prepared from the purified lectin and the three isoforms showed different degrees (80 to 83%) of identity with the amino acid sequence belonging to a previously described high nutritional value protein from A. hypocondriacus not shown at the time to be a lectin. Furthermore, analysis of tryptic peptides obtained from ALL previously treated with peptide N-glycosidase, revealed a 93% identity with the aforementioned protein. Presence of N-glycosidically linked glycans of the oligomannosidic type and, in minor proportion, of the N-acetyllactosaminic type glycans was determined by affinity chromatography on immobilized Con A.

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Research paper thumbnail of Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil

Malaria journal, 2006

The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated i... more The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil. Seven recombinant proteins representing four vir subfamilies (A, B, C, and E) obtained from a single patient from the Amazon Region were expressed in Escherichia coli as soluble glutathione S-transferase fusion proteins. The different recombinant proteins were compared by ELISA with regard to the recognition by IgM, IgG, and IgG subclass of antibodies from 200 individuals with patent infection. The frequency of individuals that presented antibodies anti-VIR (IgM plus IgG) during the infection was 49%. The frequencies of individuals that presented IgM or IgG antibodies anti-VIR were 29.6% or 26.0%, respectively. The prevalence of IgG antibodies against recombinant VIR proteins was significantly lower than the prevalence of antibodies against the recombinant proteins representing two ...

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Research paper thumbnail of Multi-element analysis of compost by laser ablation-inductively coupled plasma mass spectrometry

Talanta, Jan 15, 2007

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been applied to multi... more Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been applied to multi-element determination in compost samples. Since compost is a heterogeneous mixture of organic and inorganic materials, the influence of sample heterogeneity on the accuracy and precision of analysis was investigated. Several parameters related to the following were studied: laser (energy, laser-beam diameter, preablation. rastering speed, carrier-gas flow rate), sample preparation (use of compacted pellets, grinding time, particle size, sample amount, length of hydraulic press treatment, position of line scan), and the ICP-MS system (quantitative versus semiquantitative analysis, matrix-matched standards and liquid standards calibration). The main causes of imprecision in sample preparation were determined to be particle size and grinding time. The effect of sample heterogeneity on precision was also evaluated by using different test samples (pellets). For Ni, Zn and Pb, the greatest co...

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Research paper thumbnail of A comparative study of speech development between deaf children with cochlear implants who have been educated with spoken or spoken+sign language

International journal of pediatric otorhinolaryngology, 2009

To compare speech development following unilateral cochlear implant (CI) between a group of preli... more To compare speech development following unilateral cochlear implant (CI) between a group of prelingually deaf children who have been educated exclusively using spoken language and another group who have used two languages (spoken and sign language). A simple group quasi-experimental design was used with a control group. The sample comprised 7 girls and 11 boys, aged between 4 and 8 years old, who received a CI between the ages of 15 months and 5 years old. The sample was divided into two groups, G1-bilingual and G2-spoken language. In both groups, aspects such as speech intelligibility, receptive vocabulary, psycho-linguistic skills, adaptive behaviour and behavioural problems were measured. The children in Group 1 (bilingual) had better verbal and manual expression whereas those in Group 2 (spoken) achieved better results in terms of speech intelligibility, auditory reception and grammatical closure. These differences were confirmed statistically using Analysis of Variance. No sign...

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Research paper thumbnail of Effect of Solution Composition on the Energy Production by Capacitive Mixing in Membrane-Electrode Assembly

The journal of physical chemistry. C, Nanomaterials and interfaces, Jan 24, 2014

In this work, we consider the extent to which the presence of multivalent ions in solution modifi... more In this work, we consider the extent to which the presence of multivalent ions in solution modifies the equilibrium and dynamics of the energy production in a capacitive cell built with ion-exchange membranes in contact with high surface area electrodes. The cell potential in open circuit (OCV) is controlled by the difference between both membrane potentials, simulated as constant volume charge regions. A theoretical model is elaborated for steady state OCV, first in the case of monovalent solutions, as a reference. This is compared to the results in multi-ionic systems, containing divalent cations in concentrations similar to those in real seawater. It is found that the OCV is reduced by about 25% (as compared to the results in pure NaCl solutions) due to the presence of the divalent ions, even in low concentrations. Interestingly, this can be related to the "uphill" transport of such ions against their concentration gradients. On the contrary, their effect on the dynamic...

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Research paper thumbnail of Resolving the abundance and air-sea fluxes of airborne microorganisms in the North Atlantic Ocean

Frontiers in microbiology, 2014

Airborne transport of microbes may play a central role in microbial dispersal, the maintenance of... more Airborne transport of microbes may play a central role in microbial dispersal, the maintenance of diversity in aquatic systems and in meteorological processes such as cloud formation. Yet, there is almost no information about the abundance and fate of microbes over the oceans, which cover >70% of the Earth's surface and are the likely source and final destination of a large fraction of airborne microbes. We measured the abundance of microbes in the lower atmosphere over a transect covering 17° of latitude in the North Atlantic Ocean and derived estimates of air-sea exchange of microorganisms from meteorological data. The estimated load of microorganisms in the atmospheric boundary layer ranged between 6 × 10(4) and 1.6 × 10(7) microbes per m(2) of ocean, indicating a very dynamic air-sea exchange with millions of microbes leaving and entering the ocean per m(2) every day. Our results show that about 10% of the microbes detected in the boundary layer were still airborne 4 days...

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Research paper thumbnail of Cryobiopsy in the Diagnosis of Diffuse Interstitial Lung Disease: Yield and Cost-Effectiveness Analysis

Archivos de bronconeumologia, Jan 3, 2014

Assessment of patients with suspected interstitial lung disease (ILD) includes surgical lung biop... more Assessment of patients with suspected interstitial lung disease (ILD) includes surgical lung biopsy (SLB) when clinical and radiological data are inconclusive. However, cryobiopsy is acquiring an important role in the ILD diagnostic process. The objective of this study was to evaluate the diagnostic yield, safety and economic costs of the systematic use of cryobiopsy in the assessment of patients with suspected ILD. This was a retrospective observational study of patients who had undergone transbronchial cryobiopsy for evaluation of ILD from January 2011 to January 2014. The procedures were performed with a video bronchoscope using a cryoprobe for the collection of lung parenchyma specimens, which were analyzed by pathologists. Diagnostic yield, complications and economic costs of this technique were analyzed. Criobiopsy specimens from a total of 33 patients were included. A specific diagnosis was obtained in 26, producing a diagnostic yield of 79%. In 5 patients, SLB was required f...

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Research paper thumbnail of Enzymatic production of human milk fat substitutes containing palmitic and docosahexaenoic acids at sn-2 position and oleic acid at sn-1,3 positions

LWT - Food Science and Technology, 2011

This paper studies the synthesis of structured triacylglycerols (STAGs) rich in palmitic and doco... more This paper studies the synthesis of structured triacylglycerols (STAGs) rich in palmitic and docosahexaenoic acids (PA and DHA) at sn-2 position and oleic acid (OA) at sn-1,3 positions by a four step process. First, triacylglycerols (TAGs) were obtained with 63–66 mol PA/100 mol total fatty acids and 10 mol DHA/100 mol by acidolysis of tuna oil and commercial PA, catalyzed by the non-positionally specific lipase

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Research paper thumbnail of Resistencia a fármacos antituberculosos de primera línea en España durante 2010-2011. Estudio RETUBES

Archivos de Bronconeumología, 2015

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Research paper thumbnail of NEOPLASIAS INFILTRANTES DE VULVA

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Research paper thumbnail of Electric permittivity and dynamic mobility of dilute suspensions of plate-like gibbsite particles

Langmuir : the ACS journal of surfaces and colloids, Jan 2, 2015

In this work we discuss the electrokinetic evaluation of model plate-like particles. By model par... more In this work we discuss the electrokinetic evaluation of model plate-like particles. By model particles we mean homogeneous and controlled size and shape. The electrokinetic analysis in such complex geometries cannot be limited to a single data point as in usual electrophoresis in constant (dc) fields. The information can be made much richer if alternating (ac) fields with a sufficiently wide range of frequencies are used. In this case, two techniques can be applied: one is the determination of the frequency spectrum of the electric permittivity or dielectric constant (low-frequency dielectric dispersion), and the other is electroacoustics of suspensions and determination of the frequency dispersion of the electrophoretic mobility (dynamic or ac mobility). In this work, these techniques are used with planar gibbsite (γ-Al(OH)3) particles, modeled as oblate spheroids with a small aspect ratio. As in other laminar minerals, a particular charge distribution, differing between edges and...

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Research paper thumbnail of Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation

Molecular and cellular biology, 1999

Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene... more Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells u...

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Research paper thumbnail of Structure-Function Dissection of Myxococcus xanthus CarD N-Terminal Domain, a Defining Member of the CarD_CdnL_TRCF Family of RNA Polymerase Interacting Proteins

PLOS ONE, 2015

Two prototypes of the large CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-binding prot... more Two prototypes of the large CarD_CdnL_TRCF family of bacterial RNA polymerase (RNAP)-binding proteins, Myxococcus xanthus CarD and CdnL, have distinct functions whose molecular basis remain elusive. CarD, a global regulator linked to the action of several extracytoplasmic function (ECF) σ-factors, binds to the RNAP β subunit (RNAP-β) and to protein CarG via an N-terminal domain, CarDNt, and to DNA via an intrinsically unfolded C-terminal domain resembling eukaryotic high-mobility-group A (HMGA) proteins. CdnL, a CarDNt-like protein that is essential for cell viability, is implicated in σA-dependent rRNA promoter activation and interacts with RNAP-β but not with CarG. While the HMGA-like domain of CarD by itself is inactive, we find that CarDNt has low but observable ability to activate ECF σ-dependent promoters in vivo, indicating that the C-terminal DNA-binding domain is required to maximize activity. Our structure-function dissection of CarDNt reveals an N-terminal, five-stranded β -sheet Tudor-like domain, CarD1-72, whose structure and contacts with RNAP-β mimic those of CdnL. Intriguingly, and in marked contrast to CdnL, CarD mutations that disrupt its interaction with RNAP-β did not annul activity. Our data suggest that the CarDNt C-terminal segment, CarD61-179, may be structurally distinct from its CdnL counterpart, and that it houses at least two distinct and crucial function determinants: (a) CarG-binding, which is specific to CarD; and (b) a basic residue stretch, which is also conserved and functionally required in CdnL. This study highlights the evolution of shared and divergent interactions in similar protein modules that enable the distinct activities of two related members of a functionally important and widespread bacterial protein family.

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Research paper thumbnail of Participation of N-methyl-d-aspartate receptors on methylmercury-induced DNA damage in rat frontal cortex

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Patología cerebro-vascular hemorrágica en adultos asociada al consumo de cocaína

Cuadernos De Medicina Forense, 2005

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Research paper thumbnail of Transitory T-Lymphoblastic Leukemoid Reaction in a Neonate with Down Syndrome

Journal of Pediatric Hematology Oncology, 1990

A transient leukemoid reaction in a neonate with Down syndrome is reported. The blastic prolifera... more A transient leukemoid reaction in a neonate with Down syndrome is reported. The blastic proliferation was identified as T lymphoblastic in an early stage of maturation (prethymocytes) using morphological, cytochemical, and immunological methods. A spontaneous complete remission occurred in 8 weeks. No additional cytogenetic alterations were found, except for those concerning chromosome 21. Other cases reported in the literature reveal that cytogenetic studies may be useful to distinguish these transient leukemic reactions from true leukemias in newborns with Down syndrome. The in vitro growth pattern of peripheral blood and bone marrow may also be useful for this purpose.

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Research paper thumbnail of Studies on age determination and growth pattern of the wedge sole Dicologoglossa cuneata (Moreau, 1881) in the Spanish waters of the Gulf of Cadiz (southwest Iberian Peninsula)

The wedge sole Dicologoglossa cuneata (Moreau, 1881) is a typical coastal soleid which is con- si... more The wedge sole Dicologoglossa cuneata (Moreau, 1881) is a typical coastal soleid which is con- sidered as one of the main target species in the demersal fishery of Spain's southern Atlantic region. This paper presents a study on the growth pattern of wedge sole in the Gulf of Cadiz. Age was determined by observing and analysing whole otoliths (sagittae), thereby

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Research paper thumbnail of P-322 Treatment of chronic hepatitis C with interferon beta

International Hepatology Communications, 1995

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Research paper thumbnail of Monitoring the process to obtain red wine enriched in resveratrol and piceatannol without quality loss

Food Chemistry, 2010

Stilbene-enriched wine is considered an interesting new food product with added value as a conseq... more Stilbene-enriched wine is considered an interesting new food product with added value as a consequence of the numerous health-promoting properties ascribed to it, mainly from its trans-resveratrol content. Postharvest grapes have been treated with ultraviolet-C light to produce stilbene-enriched grapes that were later used in a conventional winemaking process to obtain a red wine enriched in stilbenes.By measuring oenological parameters

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Research paper thumbnail of A EUROPEAN NUCLEAR DATA PROGRAMME FOR ACCELERATOR-DRIVEN SYSTEMS

In the HINDAS programme, nuclear data in the 20-2 000 MeV range are evaluated by means of a combi... more In the HINDAS programme, nuclear data in the 20-2 000 MeV range are evaluated by means of a combination of nuclear models and well-selected intermediate- and high-energy experiments. A panoply of European accelerators is utilised to provide complete sets of experimental data for iron, lead and uranium over a large energy range. Nuclear model codes are being improved and validated

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Research paper thumbnail of Chemical characterization of the lectin from Amaranthus leucocarpus syn. hypocondriacus by 2-D proteome analysis

Glycoconjugate Journal, 2001

In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amara... more In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45% of \bibeta-sheet and 5% of \bialpha-helix. Amino acid sequence of the purified lectin and its isoforms was determined from peptides obtained after trypsin digestion by MALDI-TOF (Matrix assisted laser desorption ionization-time of flight). The tryptic peptides prepared from the purified lectin and the three isoforms showed different degrees (80 to 83%) of identity with the amino acid sequence belonging to a previously described high nutritional value protein from A. hypocondriacus not shown at the time to be a lectin. Furthermore, analysis of tryptic peptides obtained from ALL previously treated with peptide N-glycosidase, revealed a 93% identity with the aforementioned protein. Presence of N-glycosidically linked glycans of the oligomannosidic type and, in minor proportion, of the N-acetyllactosaminic type glycans was determined by affinity chromatography on immobilized Con A.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil

Malaria journal, 2006

The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated i... more The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil. Seven recombinant proteins representing four vir subfamilies (A, B, C, and E) obtained from a single patient from the Amazon Region were expressed in Escherichia coli as soluble glutathione S-transferase fusion proteins. The different recombinant proteins were compared by ELISA with regard to the recognition by IgM, IgG, and IgG subclass of antibodies from 200 individuals with patent infection. The frequency of individuals that presented antibodies anti-VIR (IgM plus IgG) during the infection was 49%. The frequencies of individuals that presented IgM or IgG antibodies anti-VIR were 29.6% or 26.0%, respectively. The prevalence of IgG antibodies against recombinant VIR proteins was significantly lower than the prevalence of antibodies against the recombinant proteins representing two ...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Multi-element analysis of compost by laser ablation-inductively coupled plasma mass spectrometry

Talanta, Jan 15, 2007

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been applied to multi... more Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been applied to multi-element determination in compost samples. Since compost is a heterogeneous mixture of organic and inorganic materials, the influence of sample heterogeneity on the accuracy and precision of analysis was investigated. Several parameters related to the following were studied: laser (energy, laser-beam diameter, preablation. rastering speed, carrier-gas flow rate), sample preparation (use of compacted pellets, grinding time, particle size, sample amount, length of hydraulic press treatment, position of line scan), and the ICP-MS system (quantitative versus semiquantitative analysis, matrix-matched standards and liquid standards calibration). The main causes of imprecision in sample preparation were determined to be particle size and grinding time. The effect of sample heterogeneity on precision was also evaluated by using different test samples (pellets). For Ni, Zn and Pb, the greatest co...

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Research paper thumbnail of A comparative study of speech development between deaf children with cochlear implants who have been educated with spoken or spoken+sign language

International journal of pediatric otorhinolaryngology, 2009

To compare speech development following unilateral cochlear implant (CI) between a group of preli... more To compare speech development following unilateral cochlear implant (CI) between a group of prelingually deaf children who have been educated exclusively using spoken language and another group who have used two languages (spoken and sign language). A simple group quasi-experimental design was used with a control group. The sample comprised 7 girls and 11 boys, aged between 4 and 8 years old, who received a CI between the ages of 15 months and 5 years old. The sample was divided into two groups, G1-bilingual and G2-spoken language. In both groups, aspects such as speech intelligibility, receptive vocabulary, psycho-linguistic skills, adaptive behaviour and behavioural problems were measured. The children in Group 1 (bilingual) had better verbal and manual expression whereas those in Group 2 (spoken) achieved better results in terms of speech intelligibility, auditory reception and grammatical closure. These differences were confirmed statistically using Analysis of Variance. No sign...

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Research paper thumbnail of Effect of Solution Composition on the Energy Production by Capacitive Mixing in Membrane-Electrode Assembly

The journal of physical chemistry. C, Nanomaterials and interfaces, Jan 24, 2014

In this work, we consider the extent to which the presence of multivalent ions in solution modifi... more In this work, we consider the extent to which the presence of multivalent ions in solution modifies the equilibrium and dynamics of the energy production in a capacitive cell built with ion-exchange membranes in contact with high surface area electrodes. The cell potential in open circuit (OCV) is controlled by the difference between both membrane potentials, simulated as constant volume charge regions. A theoretical model is elaborated for steady state OCV, first in the case of monovalent solutions, as a reference. This is compared to the results in multi-ionic systems, containing divalent cations in concentrations similar to those in real seawater. It is found that the OCV is reduced by about 25% (as compared to the results in pure NaCl solutions) due to the presence of the divalent ions, even in low concentrations. Interestingly, this can be related to the "uphill" transport of such ions against their concentration gradients. On the contrary, their effect on the dynamic...

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Research paper thumbnail of Resolving the abundance and air-sea fluxes of airborne microorganisms in the North Atlantic Ocean

Frontiers in microbiology, 2014

Airborne transport of microbes may play a central role in microbial dispersal, the maintenance of... more Airborne transport of microbes may play a central role in microbial dispersal, the maintenance of diversity in aquatic systems and in meteorological processes such as cloud formation. Yet, there is almost no information about the abundance and fate of microbes over the oceans, which cover >70% of the Earth's surface and are the likely source and final destination of a large fraction of airborne microbes. We measured the abundance of microbes in the lower atmosphere over a transect covering 17° of latitude in the North Atlantic Ocean and derived estimates of air-sea exchange of microorganisms from meteorological data. The estimated load of microorganisms in the atmospheric boundary layer ranged between 6 × 10(4) and 1.6 × 10(7) microbes per m(2) of ocean, indicating a very dynamic air-sea exchange with millions of microbes leaving and entering the ocean per m(2) every day. Our results show that about 10% of the microbes detected in the boundary layer were still airborne 4 days...

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Research paper thumbnail of Cryobiopsy in the Diagnosis of Diffuse Interstitial Lung Disease: Yield and Cost-Effectiveness Analysis

Archivos de bronconeumologia, Jan 3, 2014

Assessment of patients with suspected interstitial lung disease (ILD) includes surgical lung biop... more Assessment of patients with suspected interstitial lung disease (ILD) includes surgical lung biopsy (SLB) when clinical and radiological data are inconclusive. However, cryobiopsy is acquiring an important role in the ILD diagnostic process. The objective of this study was to evaluate the diagnostic yield, safety and economic costs of the systematic use of cryobiopsy in the assessment of patients with suspected ILD. This was a retrospective observational study of patients who had undergone transbronchial cryobiopsy for evaluation of ILD from January 2011 to January 2014. The procedures were performed with a video bronchoscope using a cryoprobe for the collection of lung parenchyma specimens, which were analyzed by pathologists. Diagnostic yield, complications and economic costs of this technique were analyzed. Criobiopsy specimens from a total of 33 patients were included. A specific diagnosis was obtained in 26, producing a diagnostic yield of 79%. In 5 patients, SLB was required f...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Enzymatic production of human milk fat substitutes containing palmitic and docosahexaenoic acids at sn-2 position and oleic acid at sn-1,3 positions

LWT - Food Science and Technology, 2011

This paper studies the synthesis of structured triacylglycerols (STAGs) rich in palmitic and doco... more This paper studies the synthesis of structured triacylglycerols (STAGs) rich in palmitic and docosahexaenoic acids (PA and DHA) at sn-2 position and oleic acid (OA) at sn-1,3 positions by a four step process. First, triacylglycerols (TAGs) were obtained with 63–66 mol PA/100 mol total fatty acids and 10 mol DHA/100 mol by acidolysis of tuna oil and commercial PA, catalyzed by the non-positionally specific lipase

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