Maria Mó - Academia.edu (original) (raw)
Address: Madrid, Madrid, Spain
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Papers by Maria Mó
The Journal of Cell …, Jan 1, 2008
Correspondence to Francisco S á nchez-Madrid: fsanchez.hlpr@salud.madrid.org Abbreviations used i... more Correspondence to Francisco S á nchez-Madrid: fsanchez.hlpr@salud.madrid.org Abbreviations used in this paper: ACF, autocorrelation function; EAP, endothelial adhesive platform; FCS, fl uorescence correlation spectroscopy; FLIM, fl uorescence lifetime imaging microscopy; FN, fi bronectin; FRET, F ö rster resonance energy transfer; FRETeff, FRET effi ciency; GPI, glycosylphosphatidylinositol; HUVEC, human umbilical vein endothelial cell; knn, k nearest neighbor; LEL, large extracellular loop; mEGFP, monomeric EGFP; TEM, tetraspanin-enriched microdomain; VE-cadherin, vascular endothelial cadherin.
Cellular and Molecular …, Jan 1, 2011
ADAM17/TACE is a metalloproteinase responsible for the shedding of the proinflammatory cytokine T... more ADAM17/TACE is a metalloproteinase responsible for the shedding of the proinflammatory cytokine TNF-α and many other cell surface proteins involved in development, cell adhesion, migration, differentiation, and proliferation. Despite the important biological function of ADAM17, the mechanisms of regulation of its metalloproteinase activity remain largely unknown. We report here that the tetraspanin CD9 and ADAM17 partially co-localize on the surface of endothelial and monocytic cells. In situ proximity ligation, co-immunoprecipitation, crosslinking, and pull-down experiments collectively demonstrate a direct association between these molecules. Functional studies reveal that treatment with CD9-specific antibodies or neoexpression of CD9 exert negative regulatory effects on ADAM17 sheddase activity. Conversely, CD9 silencing increased the activity of ADAM17 against its substrates TNF-α and ICAM-1. Taken together, our results show that CD9 associates with ADAM17 and, through this interaction, negatively regulates the sheddase activity of ADAM17.
Tetraspanins associate with several transmembrane proteins forming microdomains involved in inter... more Tetraspanins associate with several transmembrane proteins forming microdomains involved in intercellular adhesion and migration. Here, we show that endothelial tetraspanins relocalize to the contact site with transmigrating leukocytes and associate laterally with both ICAM-1 and VCAM-1.
The Journal of Cell …, Jan 1, 2008
Correspondence to Francisco S á nchez-Madrid: fsanchez.hlpr@salud.madrid.org Abbreviations used i... more Correspondence to Francisco S á nchez-Madrid: fsanchez.hlpr@salud.madrid.org Abbreviations used in this paper: ACF, autocorrelation function; EAP, endothelial adhesive platform; FCS, fl uorescence correlation spectroscopy; FLIM, fl uorescence lifetime imaging microscopy; FN, fi bronectin; FRET, F ö rster resonance energy transfer; FRETeff, FRET effi ciency; GPI, glycosylphosphatidylinositol; HUVEC, human umbilical vein endothelial cell; knn, k nearest neighbor; LEL, large extracellular loop; mEGFP, monomeric EGFP; TEM, tetraspanin-enriched microdomain; VE-cadherin, vascular endothelial cadherin.
Cellular and Molecular …, Jan 1, 2011
ADAM17/TACE is a metalloproteinase responsible for the shedding of the proinflammatory cytokine T... more ADAM17/TACE is a metalloproteinase responsible for the shedding of the proinflammatory cytokine TNF-α and many other cell surface proteins involved in development, cell adhesion, migration, differentiation, and proliferation. Despite the important biological function of ADAM17, the mechanisms of regulation of its metalloproteinase activity remain largely unknown. We report here that the tetraspanin CD9 and ADAM17 partially co-localize on the surface of endothelial and monocytic cells. In situ proximity ligation, co-immunoprecipitation, crosslinking, and pull-down experiments collectively demonstrate a direct association between these molecules. Functional studies reveal that treatment with CD9-specific antibodies or neoexpression of CD9 exert negative regulatory effects on ADAM17 sheddase activity. Conversely, CD9 silencing increased the activity of ADAM17 against its substrates TNF-α and ICAM-1. Taken together, our results show that CD9 associates with ADAM17 and, through this interaction, negatively regulates the sheddase activity of ADAM17.
Tetraspanins associate with several transmembrane proteins forming microdomains involved in inter... more Tetraspanins associate with several transmembrane proteins forming microdomains involved in intercellular adhesion and migration. Here, we show that endothelial tetraspanins relocalize to the contact site with transmigrating leukocytes and associate laterally with both ICAM-1 and VCAM-1.