Maria Malzone - Academia.edu (original) (raw)

Papers by Maria Malzone

Research paper thumbnail of Allevamento intensivo, in un sistema a circuito chiuso, di copepodi Calanoidi autoctoni come dieta viva alternativa per larvicoltura di specie ittiche marine

... Bengtson, 1995). Inoltre ci sono evidenze che i fosfolipidi della dieta possano contribuire a... more ... Bengtson, 1995). Inoltre ci sono evidenze che i fosfolipidi della dieta possano contribuire al consumo del cibo in modo migliore (Koven et al., 1998), agire come emulsionanti nell'intestino (Koven et al., 1993), ridurre la perossidazione lipidica ...

Research paper thumbnail of LncRNA HOTAIR as prognostic circulating marker and potential therapeutic target in patients with tumor diseases

Current Drug Targets, 2015

In recent years the importance of the role played by non-coding RNA on the regulation of gene exp... more In recent years the importance of the role played by non-coding RNA on the regulation of gene expression was increased by numerous studies. The research mainly focused on small ncRNAs, such as miRNAs, while the functions of long non-coding RNA (lncRNA) have been much less studied. lncRNAs can be transcribed from intergenic, intragenic or specific chromosomal regions. Compared to miRNAs, lncRNAs have a complex secondary and tertiary structure which allows to bind proteins, RNA, DNA and to carry out their regulatory functions. Several studies showed that extracellular ncRNAs can circulate in the blood of both healthy and diseased patients. Most of the circulating ncRNAs are included in lipid or lipoprotein vesicles, such as apoptotic bodies, macrovesicles or exosomes, in which they are highly stable. The presence of circulating ncRNAs in the blood of cancer patients versus normal subjects suggested the possibility that these molecules may represent new diagnostic markers. HOTAIR is a HOX transcript antisense RNA, located in the HOXC locus, able to repress transcription in the posterior region of the HOXD locus. HOTAIR has been <involved in the evolution of several primary tumors, wherein increase of HOTAIR expression has endorsed invasion and metastasis. In this review, we describe the experimental evidences on the potential role as circulating marker of lncRNA HOTAIR.

Research paper thumbnail of Castleman's disease of a submandibular mass diagnosed on Fine Needle Cytology: Report of a case with histopathological, immunocytochemical and imaging correlations

Intractable & Rare Diseases Research, 2016

Castleman's disea... more Castleman's disease (CD) is an unusual inflammatory lymphoproliferative disorder of uncertain aetiology, mainly involving lymphatic tissue in the mediastinum, but also occurring in the neck, lung, abdomen, pelvis, skeletal muscle and retroperitoneum. Fine Needle Cytology (FNC) is a quick, cost-effective and safe diagnostic modality to investigate on organs involved by CD, also providing a guide to treatment and management of patients with lymphoadenopathy. We report a case of a 44-year-old man who underwent FNC of a submandibular mass with subsequent surgical excision. Cytology revealed an atypical lymphoproliferative process, which arose the suspicion of CD. Histopathological study of the excised masses combined with immunhistochemistry and imaging of the submandibular and neck areas, confirmed the suspicion. A final diagnosis of Unicentric Castleman's disease, hyaline-vascular type, was made.

Research paper thumbnail of Cytological and Immunocytochemical Features of Merkel Cell Carcinoma on Fine Needle Cytology Samples: A Study of 22 Cases

Endocrine Pathology, 2015

Merkel cell carcinoma (MCC) is an uncommon neuroendocrine small cell tumor derived from the trans... more Merkel cell carcinoma (MCC) is an uncommon neuroendocrine small cell tumor derived from the transformation of the homonymous cells in the basal layer of the epidermis. MCC has a generally aggressive course, with a high tendency for local recurrence, lymph node involvement, and distant metastasis. Fine needle cytology (FNC) and immunocytochemistry were used as diagnostic procedures for 22 cases of MCC presented at our institute. All cases of MCC were successfully diagnosed on FNC. Among all of the monoclonal antisera used (CD56, CK20, CK MNF116, neuron-specific enolase (NSE), synaptophysin, and chromogranin), NSE and CD56 showed the highest frequency of positivity. The accuracy of the cytological diagnosis was 100 % compared to the corresponding previous or subsequent pathological diagnoses. FNC and immunocytochemistry represent excellent and accurate diagnostic methods to distinguish MCC from other small-cell malignant entities.

Research paper thumbnail of Combined Papillary and Mucoepidermoid Carcinoma of the Thyroid Gland: a Possible Collision Tumor Diagnosed on Fine-Needle Cytology. Report of a Case with Immunocytochemical and Molecular Correlations

Endocrine Pathology, 2015

Fine-needle cytology (FNC) is frequently used to diagnose thyroid nodules discovered by palpation... more Fine-needle cytology (FNC) is frequently used to diagnose thyroid nodules discovered by palpation or imaging studies. Molecular tests on FNC material may increase its diagnostic accuracy. We report a case of a classic papillary thyroid carcinoma combined with a mucoepidermoid carcinoma correctly identified on FNC. The papillary component had a classic immunophenotype (CK19+, TTF1+), while the mucoepidermoid one was only focally CK19+. Point mutations (BRAF and RAS) and rearrangements (RET/PTC) of the papillary component have been also investigated on FNC samples, with resulting concurrent rearrangements of RET/PTC1 and RET/PTC3, but no point mutations. The histogenesis of combined papillary and mucoepidermoid carcinoma of the thyroid still remains partly unsettled, and further genomic studies are needed to shed some more light on this peculiar neoplasm.

Research paper thumbnail of Hyperammina micaceus sp. nov.: a new foraminiferan species (Protista) from the Porcupine Abyssal Plain, northeast Atlantic

Journal of Micropalaeontology, 2004

Research paper thumbnail of Preliminary trials to rear the copepod Temora stylifera as food for fish larvae

Italian Journal of Animal Science, 2010

Research paper thumbnail of Decadal-scale changes in shallow-infaunal foraminiferal assemblages at the Porcupine Abyssal Plain, NE Atlantic

Deep Sea Research Part II: Topical Studies in Oceanography, 2010

Trends in the abundance, diversity and taxonomic composition of ‘live’ (rose Bengal stained) fora... more Trends in the abundance, diversity and taxonomic composition of ‘live’ (rose Bengal stained) foraminiferal assemblages (0-1cm layer, >63-μm fraction) were analysed in replicate multiple corer samples collected at the Porcupine Abyssal Plain (48° 50’ N, 16° 30’ W, 4850m water depth) over a 13-yr period (1989-2002). Total densities were significantly higher in 1996-2002 compared to 1989-1994, a change coincident with

Research paper thumbnail of Silicone lymphadenopathy: Presentation of a further case containing asteroid bodies on fine-needle cytology sample

Diagnostic Cytopathology, 2014

Silicone lymphadenopathy is a recognized complication of breast augmentation. It is thought to oc... more Silicone lymphadenopathy is a recognized complication of breast augmentation. It is thought to occur when silicone droplets migrate from breast implants to lymph nodes. We report the cytologic findings in axillary and inguinal lymph node aspirate smears from a 35-year-old Italian woman, who came to our observation 10 years after bilateral cosmetic breast augmentation. A fine-needle cytology of the axillary lymph node showed extensive granulomatous inflammation, numerous histiocytes, and multinucleated giant cells containing star-shaped structures known as "asteroid bodies." The inguinal lymph node aspirate simply showed an aspecific reactive hyperplasia. No evidence of malignancy was present in any of the smears as well as in the excised axillary lymph node. Diagn. Cytopathol. 2014;00:000-000. V C 2014 Wiley Periodicals, Inc.

Research paper thumbnail of Detection of Predictive Markers for Therapeutic Stratification of Salivary Glands Tumors

Current Drug Targets, 2014

Malignant tumors of salivary glands are rare lesions, often with poor prognosis. At present, surg... more Malignant tumors of salivary glands are rare lesions, often with poor prognosis. At present, surgery represents the only therapeutic choice, whereas chemotherapy is employed as palliative treatment in advanced disease.

Research paper thumbnail of Experimental cultivation of the Mediterranean calanoid copepods Temora stylifera and Centropages typicus in a pilot re-circulating system

Aquaculture Research, 2012

A pilot re-circulating system was used for the cultiva

Research paper thumbnail of Brooke-Spiegler syndrome presenting multiple concurrent cutaneous and parotid gland neoplasms: Cytologic findings on fine-needle sample and description of a novel mutation of the CYLD gene

Diagnostic Cytopathology, 2015

Multiple dermal cylindromas and membranous basal cell adenoma of parotid gland in a 67-year-old w... more Multiple dermal cylindromas and membranous basal cell adenoma of parotid gland in a 67-year-old woman with Brooke-Spiegler syndrome (BSS) were examined by fine-needle cytology. Histology, immunochemistry, and CYLD germline mutation testing were also performed. Cytomorphology and immunochemistry of the two lesions showed basaloid neoplasms, remarkably similar, composed by proliferating epithelial cells of basal type accompanied by a smaller proportion of myoepithelial cells. CYLD gene showed a novel germline splice acceptor site mutation (c.2042-1G>C) with skipping of the entire exon 15. The occurrence of analogous tumors, dermal cylindromas, and membranous basal cell adenoma of the parotid gland, in the same patient may result from the action of a single gene on ontogenetically similar stem cells. Therefore, patients with BSS should be offered a genetic counselling for an early and correct diagnosis.

Research paper thumbnail of Allevamento intensivo, in un sistema a circuito chiuso, di copepodi Calanoidi autoctoni come dieta viva alternativa per larvicoltura di specie ittiche marine

... Bengtson, 1995). Inoltre ci sono evidenze che i fosfolipidi della dieta possano contribuire a... more ... Bengtson, 1995). Inoltre ci sono evidenze che i fosfolipidi della dieta possano contribuire al consumo del cibo in modo migliore (Koven et al., 1998), agire come emulsionanti nell'intestino (Koven et al., 1993), ridurre la perossidazione lipidica ...

Research paper thumbnail of LncRNA HOTAIR as prognostic circulating marker and potential therapeutic target in patients with tumor diseases

Current Drug Targets, 2015

In recent years the importance of the role played by non-coding RNA on the regulation of gene exp... more In recent years the importance of the role played by non-coding RNA on the regulation of gene expression was increased by numerous studies. The research mainly focused on small ncRNAs, such as miRNAs, while the functions of long non-coding RNA (lncRNA) have been much less studied. lncRNAs can be transcribed from intergenic, intragenic or specific chromosomal regions. Compared to miRNAs, lncRNAs have a complex secondary and tertiary structure which allows to bind proteins, RNA, DNA and to carry out their regulatory functions. Several studies showed that extracellular ncRNAs can circulate in the blood of both healthy and diseased patients. Most of the circulating ncRNAs are included in lipid or lipoprotein vesicles, such as apoptotic bodies, macrovesicles or exosomes, in which they are highly stable. The presence of circulating ncRNAs in the blood of cancer patients versus normal subjects suggested the possibility that these molecules may represent new diagnostic markers. HOTAIR is a HOX transcript antisense RNA, located in the HOXC locus, able to repress transcription in the posterior region of the HOXD locus. HOTAIR has been <involved in the evolution of several primary tumors, wherein increase of HOTAIR expression has endorsed invasion and metastasis. In this review, we describe the experimental evidences on the potential role as circulating marker of lncRNA HOTAIR.

Research paper thumbnail of Castleman's disease of a submandibular mass diagnosed on Fine Needle Cytology: Report of a case with histopathological, immunocytochemical and imaging correlations

Intractable & Rare Diseases Research, 2016

Castleman's disea... more Castleman's disease (CD) is an unusual inflammatory lymphoproliferative disorder of uncertain aetiology, mainly involving lymphatic tissue in the mediastinum, but also occurring in the neck, lung, abdomen, pelvis, skeletal muscle and retroperitoneum. Fine Needle Cytology (FNC) is a quick, cost-effective and safe diagnostic modality to investigate on organs involved by CD, also providing a guide to treatment and management of patients with lymphoadenopathy. We report a case of a 44-year-old man who underwent FNC of a submandibular mass with subsequent surgical excision. Cytology revealed an atypical lymphoproliferative process, which arose the suspicion of CD. Histopathological study of the excised masses combined with immunhistochemistry and imaging of the submandibular and neck areas, confirmed the suspicion. A final diagnosis of Unicentric Castleman's disease, hyaline-vascular type, was made.

Research paper thumbnail of Cytological and Immunocytochemical Features of Merkel Cell Carcinoma on Fine Needle Cytology Samples: A Study of 22 Cases

Endocrine Pathology, 2015

Merkel cell carcinoma (MCC) is an uncommon neuroendocrine small cell tumor derived from the trans... more Merkel cell carcinoma (MCC) is an uncommon neuroendocrine small cell tumor derived from the transformation of the homonymous cells in the basal layer of the epidermis. MCC has a generally aggressive course, with a high tendency for local recurrence, lymph node involvement, and distant metastasis. Fine needle cytology (FNC) and immunocytochemistry were used as diagnostic procedures for 22 cases of MCC presented at our institute. All cases of MCC were successfully diagnosed on FNC. Among all of the monoclonal antisera used (CD56, CK20, CK MNF116, neuron-specific enolase (NSE), synaptophysin, and chromogranin), NSE and CD56 showed the highest frequency of positivity. The accuracy of the cytological diagnosis was 100 % compared to the corresponding previous or subsequent pathological diagnoses. FNC and immunocytochemistry represent excellent and accurate diagnostic methods to distinguish MCC from other small-cell malignant entities.

Research paper thumbnail of Combined Papillary and Mucoepidermoid Carcinoma of the Thyroid Gland: a Possible Collision Tumor Diagnosed on Fine-Needle Cytology. Report of a Case with Immunocytochemical and Molecular Correlations

Endocrine Pathology, 2015

Fine-needle cytology (FNC) is frequently used to diagnose thyroid nodules discovered by palpation... more Fine-needle cytology (FNC) is frequently used to diagnose thyroid nodules discovered by palpation or imaging studies. Molecular tests on FNC material may increase its diagnostic accuracy. We report a case of a classic papillary thyroid carcinoma combined with a mucoepidermoid carcinoma correctly identified on FNC. The papillary component had a classic immunophenotype (CK19+, TTF1+), while the mucoepidermoid one was only focally CK19+. Point mutations (BRAF and RAS) and rearrangements (RET/PTC) of the papillary component have been also investigated on FNC samples, with resulting concurrent rearrangements of RET/PTC1 and RET/PTC3, but no point mutations. The histogenesis of combined papillary and mucoepidermoid carcinoma of the thyroid still remains partly unsettled, and further genomic studies are needed to shed some more light on this peculiar neoplasm.

Research paper thumbnail of Hyperammina micaceus sp. nov.: a new foraminiferan species (Protista) from the Porcupine Abyssal Plain, northeast Atlantic

Journal of Micropalaeontology, 2004

Research paper thumbnail of Preliminary trials to rear the copepod Temora stylifera as food for fish larvae

Italian Journal of Animal Science, 2010

Research paper thumbnail of Decadal-scale changes in shallow-infaunal foraminiferal assemblages at the Porcupine Abyssal Plain, NE Atlantic

Deep Sea Research Part II: Topical Studies in Oceanography, 2010

Trends in the abundance, diversity and taxonomic composition of ‘live’ (rose Bengal stained) fora... more Trends in the abundance, diversity and taxonomic composition of ‘live’ (rose Bengal stained) foraminiferal assemblages (0-1cm layer, >63-μm fraction) were analysed in replicate multiple corer samples collected at the Porcupine Abyssal Plain (48° 50’ N, 16° 30’ W, 4850m water depth) over a 13-yr period (1989-2002). Total densities were significantly higher in 1996-2002 compared to 1989-1994, a change coincident with

Research paper thumbnail of Silicone lymphadenopathy: Presentation of a further case containing asteroid bodies on fine-needle cytology sample

Diagnostic Cytopathology, 2014

Silicone lymphadenopathy is a recognized complication of breast augmentation. It is thought to oc... more Silicone lymphadenopathy is a recognized complication of breast augmentation. It is thought to occur when silicone droplets migrate from breast implants to lymph nodes. We report the cytologic findings in axillary and inguinal lymph node aspirate smears from a 35-year-old Italian woman, who came to our observation 10 years after bilateral cosmetic breast augmentation. A fine-needle cytology of the axillary lymph node showed extensive granulomatous inflammation, numerous histiocytes, and multinucleated giant cells containing star-shaped structures known as "asteroid bodies." The inguinal lymph node aspirate simply showed an aspecific reactive hyperplasia. No evidence of malignancy was present in any of the smears as well as in the excised axillary lymph node. Diagn. Cytopathol. 2014;00:000-000. V C 2014 Wiley Periodicals, Inc.

Research paper thumbnail of Detection of Predictive Markers for Therapeutic Stratification of Salivary Glands Tumors

Current Drug Targets, 2014

Malignant tumors of salivary glands are rare lesions, often with poor prognosis. At present, surg... more Malignant tumors of salivary glands are rare lesions, often with poor prognosis. At present, surgery represents the only therapeutic choice, whereas chemotherapy is employed as palliative treatment in advanced disease.

Research paper thumbnail of Experimental cultivation of the Mediterranean calanoid copepods Temora stylifera and Centropages typicus in a pilot re-circulating system

Aquaculture Research, 2012

A pilot re-circulating system was used for the cultiva

Research paper thumbnail of Brooke-Spiegler syndrome presenting multiple concurrent cutaneous and parotid gland neoplasms: Cytologic findings on fine-needle sample and description of a novel mutation of the CYLD gene

Diagnostic Cytopathology, 2015

Multiple dermal cylindromas and membranous basal cell adenoma of parotid gland in a 67-year-old w... more Multiple dermal cylindromas and membranous basal cell adenoma of parotid gland in a 67-year-old woman with Brooke-Spiegler syndrome (BSS) were examined by fine-needle cytology. Histology, immunochemistry, and CYLD germline mutation testing were also performed. Cytomorphology and immunochemistry of the two lesions showed basaloid neoplasms, remarkably similar, composed by proliferating epithelial cells of basal type accompanied by a smaller proportion of myoepithelial cells. CYLD gene showed a novel germline splice acceptor site mutation (c.2042-1G>C) with skipping of the entire exon 15. The occurrence of analogous tumors, dermal cylindromas, and membranous basal cell adenoma of the parotid gland, in the same patient may result from the action of a single gene on ontogenetically similar stem cells. Therefore, patients with BSS should be offered a genetic counselling for an early and correct diagnosis.