Maria M Niza - Academia.edu (original) (raw)

Papers by Maria M Niza

Revista Brasileira De Medicina Veterinaria, 2022

Congenital biliary tree malformations are rarely described in the veterinary field. A congenital ... more Congenital biliary tree malformations are rarely described in the veterinary field. A congenital umbilicobiliary fistula associated with gallbladder agenesis was reported here for the first time in a 1-year-old male French bulldog. After contrast study, abdominal ultrasound, and histopathology, we concluded that the umbilicobiliary fistula was an aberrant duct that originated directly from the expected location of the cystic duct and gallbladder. The clinical case was treated surgically through ligation and excision of the aberrant duct before entering the common bile duct. The recovery and long-term follow-up were uneventful. The pathophysiology of biliary congenital malformations is discussed, along with clinical considerations that should be considered in similar future cases.

CBARQ sections and items translated into European Portuguese.</p

Demographic characteristics of canine population in study (N = 345).&... more Demographic characteristics of canine population in study (N = 345).</p

World Congress on Electrical Engineering and Computer Systems and Science, 2017

Cogent Biology, 2017

Purpose: Evaluate if etomidate modulates adrenal apoptosis and if this influences the development... more Purpose: Evaluate if etomidate modulates adrenal apoptosis and if this influences the development of critical illness-related corticosteroid insufficiency (CIRCI) in hemorrhagic shock (HS). Material and methods: Four groups of 16 male Wistar rats: G0 (control group anesthetized with isoflurane and mechanical ventilation), G1 (like G0, but with buprenorphine), G2 (like G1 with HS), and G3 (like G2 with etomidate 1 mg/kg, IV, before HS). HS induced by collecting 30% of blood volume. Resuscitation performed 90 min later with the collected blood and normal saline. Hemodynamic parameters, blood gas analysis, adrenocorticotropic hormone (ACTH), corticosterone (CS), and TNF-α, IL6, IL10 were determined at 0, 90, 150, and 240 min post-HS induction (at the corresponding time points in G0 and G1). Apoptosis and necrosis were determined by TUNEL and caspase-3 immunofluorescence and a necrosis score, respectively. Results: HS groups had significantly higher levels of apoptosis and necrosis than G1 and G0. Compared with G2, etomidate-treated animals had significantly lower levels of CS (compatible with CIRCI), PO 2 , PO 2 /FiO 2 , BE, HCO 3 , apoptosis, and necrosis and significantly higher cytokine levels. Conclusions: Etomidate was associated with CIRCI. HS was associated with adrenal gland

Open Veterinary Journal, 2019

The human-dog relationship is at least 16,000-years old and is mutually beneficial to both dyadic... more The human-dog relationship is at least 16,000-years old and is mutually beneficial to both dyadic members. When the human-dog relationship becomes dysfunctional, however, there can be serious consequences for both parties and for society. Unfortunately, dysfunctional dyads are normally only identified after consequences have been felt (e.g., dog-human aggression) limiting the action that can be taken to prevent such occurrences. To evaluate whether these dysfunctional dyads can be preemptively identified, a questionnaire analyzing the owners' dog health care histories was administered to an urban dog owning population. Multiple correspondence analysis (n = 1,385) was conducted and identified three clusters accounting for 37.1% of the total variance, while four moderate positive correlations were found: "unspecified trauma" with "vehicular trauma" (r = 0.303, p < 0.001), "bitten" with "bit other animal" (r = 0.345, p < 0.001), "bit a person" with "bit other animal" (r = 0.369, p < 0.001), and "chronic illness" with "hospitalized" (r = 0.297, p < 0.001). These results suggest that a simple questionnaire can identify potential characteristics of functional and dysfunctional dyads. In functional dyads, humans tend to be responsible for their dogs' well-being, while dysfunctional dyads show the opposite characteristics, reporting experience with trauma and dog aggression.

Journal of the American Veterinary Medical Association, 2018

Small Animals B rachycephalic breeds of dogs, and more recently cats, have been gaining popularit... more Small Animals B rachycephalic breeds of dogs, and more recently cats, have been gaining popularity as pets. According to the Cat Fanciers' Association, Persian and Exotic cats have ranked as the most popular purebred cats in the United States in the past few years. 1 The structure of their skulls gives these cats a unique appearance but may also result in developmental dental anomalies 2,3 that can be categorized on the basis of number, shape, structure, and position. These anomalies can cause problems on their own but can also contribute to oral and dental diseases, 4 the most commonly reported diseases in feline veterinary practice. 5,6 Thus, informa-Oral and dental anomalies in purebred, brachycephalic Persian and Exotic cats Lisa A. Mestrinho dvm, phd

$Research paper thumbnail of {"__content__"=>"Effects of buprenorphine in the adrenal, thyroid, and cytokine intra-operative responses in a rat model (): a preliminary study.", "i"=>{"__content__"=>"Rattus norvegicus"}}$

Iranian journal of basic medical sciences, 2017

Buprenorphine is a common analgesic in experimental research, due to effectiveness and having few... more Buprenorphine is a common analgesic in experimental research, due to effectiveness and having few side-effects, including a limited influence in the immune and endocrine systems. However, how buprenorphine affects cytokine levels and the adrenal and thyroid response during general anesthesia and surgery is incompletely understood. This study aimed to assess whether buprenorphine modulated significantly those responses in rats submitted to general anesthesia, mechanical ventilation, and surgical insertion of intravascular catheters. Animals were anesthetized with isoflurane, mechanically ventilated, and surgically instrumented for carotid artery and the femoral vein catheter placement. The test group (n=16), received buprenorphine subcutaneously before surgery, whereas the control group (n=16) received normal saline. Blood sampling to determine plasma levels of adrenocorticotropic hormone (ACTH), corticosterone (CS), total thyroxine (TT4), total triiodothyronine (TT3), thyroid-stimul...

Journal of veterinary dentistry, 2017

Grade and labeling indices for immunohistochemical tumor proliferation markers Ki-67 and prolifer... more Grade and labeling indices for immunohistochemical tumor proliferation markers Ki-67 and proliferating cell nuclear antigen (PCNA) were evaluated in 36 cases of canine oral squamous cell carcinoma (OSCC) based upon intraoral location. Grade was significantly associated with location ( P = .035). Grade II tumors were most frequently diagnosed. Grade I tumors were identified in the gingiva and the buccal mucosa, and grade III tumors were seen in the gingiva and the tonsillar region. Animals with tumors arising from the tonsils and of the tongue tended to be older ( P = .007), and those in the former group were more likely to have metastatic disease at the time of diagnosis ( P = .001). Mean expression of PCNA and Ki-67 proliferation index (PI) for all tumors were 62.54% and 50.70%, respectively, and there was a statistical significant association between the 2 variables ( R = .70; P < .001). Proliferation index was not associated with any of the intraoral locations evaluated, but h...

European Journal of Inflammation, 2016

Hemorrhagic shock (HS) induces a compensatory endocrine and cytokine response which aims to resto... more Hemorrhagic shock (HS) induces a compensatory endocrine and cytokine response which aims to restore homeostasis. This response can be modulated by general anesthetics. To our knowledge, no studies have evaluated if etomidate modulates this response in experimental HS. After being premedicated with buprenorphine (0.05 mg/kg subcutaneously), male Wistar rats were anaesthetized with 5% isoflurane and divided into three groups: G1 (control, n = 16), G2 (n = 13), and G3 (n = 14). G2 and G3 were subjected to HS by collecting 30% of their blood volume and resuscitated 90 min later with the collected blood and normal saline, in a 1:3 ratio, respectively. G3 received etomidate (1 mg/kg IV) before HS. Blood gas analysis, adrenocorticotropic hormone (ACTH), corticosterone, and plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10 and of TNF-α, IL-6, and IL-10 mRNA obtained through real-time polymerase chain reaction (RT-PCR) were measured at 0, 90, 150, and 240 min after HS...

Pratique Medicale Et Chirurgicale De L Animal De Compagnie, 2005

Cette etude decrit les consequences cliniques et hematologiques de l'administration d'un ... more Cette etude decrit les consequences cliniques et hematologiques de l'administration d'un facteur recombinant stimulant les colonies de granulocytes humains (rhG-CSF) a sept chats naturellement infectes par le virus de la panleucopenie. La maladie s'est developpee dans une colonie de chats d'interieur non vaccines, apres l'introduction d'un animal infecte. En plus d'un traitement de soutien, le rhG-CSF a ete administre une fois par jour (5 μg/kg), pendant 5 jours, et tous les chats, sauf un, ont survecu. Les analyses de sang ont ete realisees avant le debut du traitement, 24 heures apres la premiere injection, et 24 heures et 5 jours apres la fin du traitement. Une augmentation marquee de la quantite de neutrophiles a ete constatee dans les premieres 24 heures chez tous les chats ayant survecu. Cinq jours apres la fin de l'administration de la cytokine, tous les chats avaient des valeurs normales de leucocytes, a l'exception d'un chat qui avait une leucocytose avec neutrophilie. La cytokine rhG-CSF a ete bien toleree et aucun effet secondaire n'a ete constate. Ces resultats suggerent que la rhG-CSF pourrait etre utilisee en toute securite chez le Chat, avec des effets benefiques dans le traitement de l'infection par le virus de la panleucopenie.

Journal of Veterinary Science & Technology, 2015

Background In humans and rats the peritoneal surface area is described, but not the abdominal cav... more Background In humans and rats the peritoneal surface area is described, but not the abdominal cavity surface area (ACSA). On the literature review undertaken, no discriminatory values of surface area of the abdominal cavity in cats were found. The objective of the study is to determine the ACSA in cats, through three-dimensional (3D) modeling. For this purpose it was performed a retrospective study of a series of cases of tomographic exams of cats, without changes in the abdominal cavity. Processing the digital images and through segmentation techniques was constructed the 3D model of the abdominal cavity of 26 cats, making it possible to determine its volume and surface area. Results The volume (mean ± standard deviation) of the abdominal cavity of cats was 6.59x10-4 ± 2.22 × 10-4 m 3 , and the area of abdominal surface 5.11x10-2 ± 9.86 × 10-3 m 2. Conclusions The values determined provide not-existent anatomical information and contribute to the study of diseases affecting this area. The determination of this area allows its application in calculating and determining intraabdominal pressures and understanding them better. The same methodology can be used in other species for the determination and calculation of the abdominal surface area.

Journal of Feline Medicine and Surgery Open Reports, 2015

Case summary A 2-year-old, intact female domestic longhair cat was referred for surgical treatmen... more Case summary A 2-year-old, intact female domestic longhair cat was referred for surgical treatment after diagnosis of closed jaw locking secondarily to right temporomandibular joint ankylosis and left pseudoankylosis. The animal underwent successful surgical management with bilateral excision arthroplasty followed by interposition of a temporal superficial myofascial flap. Immediately after surgery, the full range of lower jaw movement was achieved and normal occlusion was maintained. Ankylosis did not recur in the 1 year postoperative follow-up period. Relevance and novel information A temporal myofascial flap could be considered as interposition material after temporomandibular joint arthroplasty to avoid postoperative re-ankylosis and mandibular drift. The main advantages of this flap are its autogenous origin, and the ability to maintain separation between the two bones, preserve mobility and disrupt new bone formation.

Research in Veterinary Science, 2015

This study assesses viremia, provirus and blood cytokine profile in naturally FIV-infected cats t... more This study assesses viremia, provirus and blood cytokine profile in naturally FIV-infected cats treated with two distinct protocols of interferon omega (rFeIFN-ω). Samples from FIV-cats previously submitted to two single-arm studies were used: 7/18 received the licensed/subcutaneous protocol (SC) while 11/18 were treated orally (PO). Viremia, provirus and blood mRNA expression of interleukin (IL)-1, IL-4, IL-6, IL-10, IL-12p40, Interferon-γ and Tumor Necrosis Factor-α were monitored by Real-Time qPCR. Concurrent plasma levels of IL-6, IL-12p40 and IL-4 were assessed by ELISA. IL-6 plasma levels decreased in the SC group (p = 0.031). IL-6 mRNA expression (p = 0.037) decreased in the PO group, albeit not sufficiently to change concurrent plasma levels. Neither viremia nor other measured cytokines changed with therapy. Proviral load increased in the SC group (p = 0.031), which can be justified by a clinically irrelevant increase of lymphocyte count. Independently of the protocol, rFeIFN-ω seems to act on innate immunity by reducing proinflammatory stimulus.

Veterinary Pathology, 2014

The expression of p63 and E-cadherin was studied in 22 oral squamous cell carcinomas in the dog a... more The expression of p63 and E-cadherin was studied in 22 oral squamous cell carcinomas in the dog according to immunohistochemical techniques. The association between these markers and clinicopathologic parameters was assessed. All tumor cells studied showed enhanced p63 expression. Regarding E-cadherin expression, 17 of 22 cases (77.3%) showed decreased immunoreactivity, and in 13 of 22 cases (59.1%), its expression was cytoplasmic. Neither p63 nor E-cadherin expression patterns were associated with tumor size, bone invasion, or lymph node metastasis. p63 score was related to proliferating cell nuclear antigen proliferative index ( P = .020). A statistically significant correlation between the expression patterns of these 2 markers was noted ( P = .026). Furthermore, they were related with tumor grade. An atypical p63 labeling and a cytoplasmic E-cadherin staining were statistically related with a higher tumor grade ( P = .022 and P = .017, respectively). These findings suggest that ...

Veterinary Microbiology, 2010

Although dog-to-dog bite wounds are frequent, few studies correlate bacterial involvement to clin... more Although dog-to-dog bite wounds are frequent, few studies correlate bacterial involvement to clinical aspects. This work aimed at relating clinical evolution and bacteriological data, with the evolution time (ET), i.e., the period of time elapsed from aggression until presentation. A total of 228 wounds from 83 cases of bitten dogs was evaluated; 48 of the wounds were sampled for bacteriology. Dogs with clinically infected wounds (N=29) were subjected to antimicrobial therapy and local disinfection. Dogs without clinical signs of infection were either subjected to the same treatment (N=43) or only subjected to daily wound saline irrigation (N=11), to evaluate the need for antimicrobial prophylaxis. The majority of wounds were laceration and puncture wounds with dermis penetration (41.2% and 23.2%, respectively). Only 17% of the wounds were clinically infected. The mean ET was 39h 30m and prolonged ET was significantly correlated to infection. None of the wounds from animals not given antibiotics became infected. Bacteriology was positive in 95.8% of the wounds sampled (N=46). A total of 125 isolates was obtained, mostly aerobes. Clinical infection was associated with the presence of strict anaerobes. Excluding antibiotics that should be preserved for life-threatening cases, the higher rates of in vitro antimicrobial susceptibility were observed for sulphamethoxazole-trimethoprim (94.4%) and amoxicillin-clavulanate (91.9%). This study identified the time lag from aggression to presentation as a risk factor for infection development. Further studies are required to evaluate the actual requirement for antimicrobial therapy when only dermis is affected in dog-to-dog bite wounds.

Research in Veterinary Science, 2014

Recombinant-Feline Interferon-Omega (rFeIFN-x) is an immune-modulator licensed for use subcutaneo... more Recombinant-Feline Interferon-Omega (rFeIFN-x) is an immune-modulator licensed for use subcutaneously in Feline Immunodeficiency virus (FIV) therapy. Despite oral protocols have been suggested, little is known about such use in FIV-infected cats. This study aimed to evaluate the clinical improvement, laboratory findings, concurrent viral excretion and acute phase proteins (APPs) in naturally FIV-infected cats under oral rFeIFN-x therapy (0.1 MU/cat rFeIFN-x PO, SID, 90 days). 11 FIV-positive cats were treated with oral rFeIFN-x (PO Group). Results were compared to previous data from 7 FIV-positive cats treated with the subcutaneous licensed protocol (SC Group). Initial clinical scores were similar in both groups. Independently of the protocol, rFeIFN-x induced a significant clinical improvement of treated cats. Concurrent viral excretion and APP's variation were not significant in the PO Group. Oral rFeIFN-x can be an effective alternative therapy for FIV-infected cats, being also an option for treatment follow-up in cats submitted to the licensed protocol.

Journal of Small Animal Practice, 2004

Journal of Small Animal Practice, 2013

¶These authors contributed equally to the work. OBJECTIVES: Recombinant feline interferon-v thera... more ¶These authors contributed equally to the work. OBJECTIVES: Recombinant feline interferon-v therapy is an immunomodulator currently used in the treatment of different retroviral diseases including feline immune deficiency virus and feline leukaemia virus. Although its mechanism of action remains unclear, this drug appears to potentiate the innate response. Acute phase proteins are one of the key components of innate immunity and studies describing their use as a monitoring tool for the immune system in animals undergoing interferon-v therapy are lacking. This study aimed to determine whether interferon-v therapy influences acute phase protein concentrations namely serum amyloid-A, `-1-glycoprotein and C-reactive protein. METHODS: A single-arm study was performed using 16 cats, living in an animal shelter, naturally infected with retroviruses and subjected to the interferon-v therapy licensed protocol. Samples were collected before (D0), during (D10 and D30) and after therapy (D65). Serum amyloid-A and C-reactive protein were measured by specific enzyme-linked immunosorbent assay kits and `-1-glycoprotein by single radial immunodiffusion. RESULTS: All the acute phase proteins significantly increased in cats undergoing interferon-v therapy (D0/D65: P<0•05) CLINICAL SIGNIFICANCE: Acute phase proteins appear to be reasonable predictors of innate-immune stimulation and may be useful in the individual monitoring of naturally retroviral infected cats undergoing interferon-v therapy.