Marian Kuczek - Academia.edu (original) (raw)

Papers by Marian Kuczek

Acta Biochimica Polonica, 2003

Chemischer Informationsdienst, Jun 2, 1981

ChemInform Abstract Die Titelreaktion mit Anilin, 4-Methoxy-und 4-Bromanilin in methanolischen Pu... more ChemInform Abstract Die Titelreaktion mit Anilin, 4-Methoxy-und 4-Bromanilin in methanolischen Puffer-% lösungen zu den entsprechenden Isobutylidenanilinen wird untersucht. Es werden die i Brönsted-Koeffizienten o: und die Hammett-Reaktionskonstanten p der Bildung und rc Dehydratisierung der Carbinolamin-Zwischenstufe angegeben.

Chemischer Informationsdienst, 1976

Anstelle von Phenylisothio cyanat läßt sich für den Protein‐und Peptidabbau (Analyse der Aminosäu... more Anstelle von Phenylisothio cyanat läßt sich für den Protein‐und Peptidabbau (Analyse der Aminosäuresequenz) vorteilhaft das Stilbenisothiocyanat (I) verwenden: Mit einem Peptid (II) z.B. entstehen so fluoreszierende Thiohydantoine wie (III) und das um eine Aminosäureeinheit ärmere Peptid (IV).

Acta biochimica Polonica, 1976

1. The amino acid compostion, N- and C-terminal amino acid sequences, and the subunit molecular w... more 1. The amino acid compostion, N- and C-terminal amino acid sequences, and the subunit molecular weight of glyceraldehyde phosphate dehydrogenase from human muscle, were determined. The obtained results and the maps of tryptic peptides suggest that the enzyme is composed of four identical or very similar polypeptide chains. 2. From the tryptic digest of performic acid-oxidized enzyme, 32 peptides were isolated. The amino acid sequence analysis showed a high degree of homology with the corresponding tryptic peptides of the dehydrogenase from pig muscle, with 9 replacements and probably two additional amino acids in the examined sequences of the human muscle enzyme.

Cellular & Molecular Biology Letters, 1998

Cellular & Molecular Biology Letters, 2002

In 1969, London and Steck proposed a general model for reactions requiring ATP and magnesium ions... more In 1969, London and Steck proposed a general model for reactions requiring ATP and magnesium ions. This model assumes the interaction of the metal cation with ATP, resulting in the formation of a complex. The simplified equation system of the London-Steck model was limited to one second order equilibrium equation for metal-substrate complex formation, and linear equations for the interactions of the metal-substrate complex, the metal and the substrate with an enzyme or with the enzyme-substrate complex. It explained the characteristics of allosteric model sigmoid curves as a result of the distribution of consecutive tertiary and quaternary complex concentration of the enzyme, metal cation and ligands, without regard to allosteric interaction. In 1979, Vinogradov and Strumilo analysed the consistency of the kinetics of the thiamine pyrophosphokinase reaction with the London-Steck model. Contrary to earlier suggestions, the authors came to the conclusion that at a certain range of sub...

Acta Biochimica Polonica, 1994

Acta biochimica Polonica, 1983

Specificity of acid protease from Fusarium moniliforme was investigated using beta chain of oxidi... more Specificity of acid protease from Fusarium moniliforme was investigated using beta chain of oxidized insulin. The enzyme is highly specific for the bonds involving aromatic and hydrophobic amino acid residues. It shows high affinity for the following bonds: Leu(15)-Tyr(16), Tyr(16)-Leu(17), Phe(24)-Phe(25), Phe(25)-Tyr(26), and somewhat lower for other three bonds: His(10)-Leu(11), Leu(11)-Val(12) and Tyr(26)-Thr(27) in oxidized beta chain of insulin.

Acta biochimica Polonica, 1977

The amino acid sequences of tryptic peptides from the performic acid-oxidized trypsin inhibitor w... more The amino acid sequences of tryptic peptides from the performic acid-oxidized trypsin inhibitor were determined. The degradation was performed with a new reagent, 3-isothiocyanato-4-methoxy-4'-nitrostilbene, and compared with the dansyl-Edman technique. The amino acid sequence of the trypsin inhibitor from bovine splenic capsule was found to be the same as that of the basic trypsin inhibitor from bovine pancreas, established by Kress & Laskowski (J. Biol. Chem. 1967, 242, 4925-4928).

The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and... more The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and enzyme interact via a metal cation and form simple and mixed, mono- and multi-nuclear complexes. A solution of equations for individual cases gives a function of initial reaction velocity at any given substrate or modifier concentration. This function can describe kinetic effects that are considered allosteric, as well as phenomena omitted by commonly-accepted models.

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1985

The labelling of erythrocyte spectrin in situ with the hydrophobic reagent phenylisothiocyanate (... more The labelling of erythrocyte spectrin in situ with the hydrophobic reagent phenylisothiocyanate (Sigrist, H. and Zahlei', P. (1978) FEBS Lett. 95, 116-120) is studied. Spectrin isolated from erythrocytes which have been incubated with phenylisothiocyanate is covalently modified by the probe. The modification in the spectrin molecule is stable under an excess of nucleophile in alkaline conditions. The labelling is very little or not affected by preincubation of erythrocytes of membranes with the polar, structural analogue of phenylisothiocyanate, p-sulfophenylisothiocyanate. When erythrocyte ghosts are subjected to labelling, a substantial increase in the degree of spectrin modification is observed. Subunits of labelled spectrin separated electrophoretically show similar amounts of attached label.

Chemischer Informationsdienst, 1976

Proceedings of the FEBS Advanced Course No. 84/07, Prague, Czechoslovakia, August 20–24, 1984, 2000

Cellular & molecular biology letters, 2003

The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and... more The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and enzyme interact via a metal cation and form simple and mixed, mono- and multi-nuclear complexes. A solution of equations for individual cases gives a function of initial reaction velocity at any given substrate or modifier concentration. This function can describe kinetic effects that are considered allosteric, as well as phenomena omitted by commonly-accepted models.

Biomedica biochimica acta, 1987

Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodopheny... more Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodophenylisothiocyanate dispersed in lipid vesicles was studied. Although spectrin is a peripheral protein of the erythrocyte membrane, it was found to be labelled as a result of incubation of washed red blood cells with hydrophobic arylisothiocyanates.

Acta Crystallographica Section C Crystal Structure Communications, 1991

Ca2+.2(C3H406P)-.2H20, Mr=410.18, triclinic, Pi, a= 5.424 (4), b=5.594(6), c= 12.078 (9) A, t~=83... more Ca2+.2(C3H406P)-.2H20, Mr=410.18, triclinic, Pi, a= 5.424 (4), b=5.594(6), c= 12.078 (9) A, t~=83.01_(8), /3 =85.73 (6), y= 0108-2701/91/081598-03503.00

Acta Crystallographica Section C Crystal Structure Communications, 1991

Acta Biochimica Polonica, 2003

Chemischer Informationsdienst, Jun 2, 1981

ChemInform Abstract Die Titelreaktion mit Anilin, 4-Methoxy-und 4-Bromanilin in methanolischen Pu... more ChemInform Abstract Die Titelreaktion mit Anilin, 4-Methoxy-und 4-Bromanilin in methanolischen Puffer-% lösungen zu den entsprechenden Isobutylidenanilinen wird untersucht. Es werden die i Brönsted-Koeffizienten o: und die Hammett-Reaktionskonstanten p der Bildung und rc Dehydratisierung der Carbinolamin-Zwischenstufe angegeben.

Chemischer Informationsdienst, 1976

Anstelle von Phenylisothio cyanat läßt sich für den Protein‐und Peptidabbau (Analyse der Aminosäu... more Anstelle von Phenylisothio cyanat läßt sich für den Protein‐und Peptidabbau (Analyse der Aminosäuresequenz) vorteilhaft das Stilbenisothiocyanat (I) verwenden: Mit einem Peptid (II) z.B. entstehen so fluoreszierende Thiohydantoine wie (III) und das um eine Aminosäureeinheit ärmere Peptid (IV).

Acta biochimica Polonica, 1976

1. The amino acid compostion, N- and C-terminal amino acid sequences, and the subunit molecular w... more 1. The amino acid compostion, N- and C-terminal amino acid sequences, and the subunit molecular weight of glyceraldehyde phosphate dehydrogenase from human muscle, were determined. The obtained results and the maps of tryptic peptides suggest that the enzyme is composed of four identical or very similar polypeptide chains. 2. From the tryptic digest of performic acid-oxidized enzyme, 32 peptides were isolated. The amino acid sequence analysis showed a high degree of homology with the corresponding tryptic peptides of the dehydrogenase from pig muscle, with 9 replacements and probably two additional amino acids in the examined sequences of the human muscle enzyme.

Cellular & Molecular Biology Letters, 1998

Cellular & Molecular Biology Letters, 2002

In 1969, London and Steck proposed a general model for reactions requiring ATP and magnesium ions... more In 1969, London and Steck proposed a general model for reactions requiring ATP and magnesium ions. This model assumes the interaction of the metal cation with ATP, resulting in the formation of a complex. The simplified equation system of the London-Steck model was limited to one second order equilibrium equation for metal-substrate complex formation, and linear equations for the interactions of the metal-substrate complex, the metal and the substrate with an enzyme or with the enzyme-substrate complex. It explained the characteristics of allosteric model sigmoid curves as a result of the distribution of consecutive tertiary and quaternary complex concentration of the enzyme, metal cation and ligands, without regard to allosteric interaction. In 1979, Vinogradov and Strumilo analysed the consistency of the kinetics of the thiamine pyrophosphokinase reaction with the London-Steck model. Contrary to earlier suggestions, the authors came to the conclusion that at a certain range of sub...

Acta Biochimica Polonica, 1994

Acta biochimica Polonica, 1983

Specificity of acid protease from Fusarium moniliforme was investigated using beta chain of oxidi... more Specificity of acid protease from Fusarium moniliforme was investigated using beta chain of oxidized insulin. The enzyme is highly specific for the bonds involving aromatic and hydrophobic amino acid residues. It shows high affinity for the following bonds: Leu(15)-Tyr(16), Tyr(16)-Leu(17), Phe(24)-Phe(25), Phe(25)-Tyr(26), and somewhat lower for other three bonds: His(10)-Leu(11), Leu(11)-Val(12) and Tyr(26)-Thr(27) in oxidized beta chain of insulin.

Acta biochimica Polonica, 1977

The amino acid sequences of tryptic peptides from the performic acid-oxidized trypsin inhibitor w... more The amino acid sequences of tryptic peptides from the performic acid-oxidized trypsin inhibitor were determined. The degradation was performed with a new reagent, 3-isothiocyanato-4-methoxy-4'-nitrostilbene, and compared with the dansyl-Edman technique. The amino acid sequence of the trypsin inhibitor from bovine splenic capsule was found to be the same as that of the basic trypsin inhibitor from bovine pancreas, established by Kress & Laskowski (J. Biol. Chem. 1967, 242, 4925-4928).

The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and... more The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and enzyme interact via a metal cation and form simple and mixed, mono- and multi-nuclear complexes. A solution of equations for individual cases gives a function of initial reaction velocity at any given substrate or modifier concentration. This function can describe kinetic effects that are considered allosteric, as well as phenomena omitted by commonly-accepted models.

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1985

The labelling of erythrocyte spectrin in situ with the hydrophobic reagent phenylisothiocyanate (... more The labelling of erythrocyte spectrin in situ with the hydrophobic reagent phenylisothiocyanate (Sigrist, H. and Zahlei', P. (1978) FEBS Lett. 95, 116-120) is studied. Spectrin isolated from erythrocytes which have been incubated with phenylisothiocyanate is covalently modified by the probe. The modification in the spectrin molecule is stable under an excess of nucleophile in alkaline conditions. The labelling is very little or not affected by preincubation of erythrocytes of membranes with the polar, structural analogue of phenylisothiocyanate, p-sulfophenylisothiocyanate. When erythrocyte ghosts are subjected to labelling, a substantial increase in the degree of spectrin modification is observed. Subunits of labelled spectrin separated electrophoretically show similar amounts of attached label.

Chemischer Informationsdienst, 1976

Proceedings of the FEBS Advanced Course No. 84/07, Prague, Czechoslovakia, August 20–24, 1984, 2000

Cellular & molecular biology letters, 2003

The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and... more The nonallosteric regulation mechanism of enzyme reaction velocity assumes that the substrate and enzyme interact via a metal cation and form simple and mixed, mono- and multi-nuclear complexes. A solution of equations for individual cases gives a function of initial reaction velocity at any given substrate or modifier concentration. This function can describe kinetic effects that are considered allosteric, as well as phenomena omitted by commonly-accepted models.

Biomedica biochimica acta, 1987

Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodopheny... more Labelling of spectrin with the hydrophobic probes 2-naphthylisothiocyanate and 4-[125I]-iodophenylisothiocyanate dispersed in lipid vesicles was studied. Although spectrin is a peripheral protein of the erythrocyte membrane, it was found to be labelled as a result of incubation of washed red blood cells with hydrophobic arylisothiocyanates.

Acta Crystallographica Section C Crystal Structure Communications, 1991

Ca2+.2(C3H406P)-.2H20, Mr=410.18, triclinic, Pi, a= 5.424 (4), b=5.594(6), c= 12.078 (9) A, t~=83... more Ca2+.2(C3H406P)-.2H20, Mr=410.18, triclinic, Pi, a= 5.424 (4), b=5.594(6), c= 12.078 (9) A, t~=83.01_(8), /3 =85.73 (6), y= 0108-2701/91/081598-03503.00

Acta Crystallographica Section C Crystal Structure Communications, 1991