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Papers by Marián Liberko

Research paper thumbnail of Circulating Tumor Cells, Circulating Tumor DNA and Other Blood-based Prognostic Scores in Pancreatic Ductal Adenocarcinoma – Mini-Review

Research paper thumbnail of Abstract 5123: In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models

Cancer Research, 2013

Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC In the present study ... more Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC In the present study circulating tumor cells (CTCs) were isolated from H460-RFP orthotopic human lung cancer model in mice. In vitro culture of CTCs has enabled us comparison of the chemosensitivity of tumor cells from primary H460 culture and of CTCs solated from blood grown in vitro. Cell line H460-RFP human lung cancer cells were grown to 70-80% confluence. Primary tumor were grown after subcutaneous injection of cultured cells. Fragments of tumor tissue (1 mm × 1 mm) were implanted into the lungs of nude mice. At 14 days, the mice were sacrificied and blood was taken by cardiac puncture for CTC-analysis. The blood (0.5 ml), obtained by cardiac puncture, was put into an EDTA tube (BD) and the CTC cells were enriched by immunomagnetic separation targeted to eptihelial antigens. The enriched fraction or whole blood was put into culture. Cell growth was then monitored by fluorescence microscopy. The isolation and culture of the fluorescent CTC will enable their further characterization. Citation Format: Katarina Kolostova, Marian Liberko, Eva Hroncova, Robert M. Hoffman, Vladimir Bobek. In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5123. doi:10.1158/1538-7445.AM2013-5123

Research paper thumbnail of Abstract 2680: Characterization of cultured circulating melanoma cells for individualized therapy

Cancer Research, 2012

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented st... more Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented study circulating melanoma cells (CMSs) have been isolated from a B16 mouse melanoma model and from melanoma patients. Culture of CMCs has enabled us to perform drug sensitivity testing on them. The main hypothesis was to compare drug sensitivity of primary tumor cells grown in culture with the sensitivity of CMCs grown in vitro after capture. B16 murine melanoma was implanted i.p. in mice. Fourteen days after peritoneal injection, mice were sacrificed and blood obtained by cardiac puncture for CMCs-capture. The CMCs were captured either by immunomagnetic separation (Adnagen, AdnaTest Melanoma Select™, Germany) and/or blood added to culture flask containing growing medium. Sensitivity to cisplatin (CDDP) was tested with the MTT-assay at different concentrations. Primary melanoma and disseminated tumor cells (DTCs) were also cultured and tested. The DTCs were isolated from peritoneal washing (ascites like cells), lymph nodes and peritoneal fat tissue. CMCs and DTCs were found less sensitive to CDDP than primary melanoma cells. The ability to capture and culture CTCs enabled these studies which can be used for individualized therapy design. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2680. doi:1538-7445.AM2012-2680

Research paper thumbnail of Complete remission and long-term response to gemcitabine + nab-paclitaxel in patient with metastatic pancreatic adenocarcinoma

Research paper thumbnail of The current role of radiotherapy and systemic therapy in the multidisciplinary treatment of cholangiocarcinoma

Casopis lekaru ceskych, 2019

Cholangiocarcinoma is a cancer with very poor prognosis. The only potentially curative approach i... more Cholangiocarcinoma is a cancer with very poor prognosis. The only potentially curative approach is surgical resection of tumor. However, the rate of local and distant recurrence after radical surgery is still high. Benefit of adjuvant therapy is not clearly defined, nevertheless patients at high risk of recurrence are indicated to chemotherapy or chemoradiotherapy. Locally advanced, unresectable disease can also be treated with chemotherapy alone, or with her combination with radiotherapy. Required radiation doses are relatively high, therefore it is necessary to use highly conformal radiation therapy. Treatment of metastatic disease is currently based on systemic therapy, combination of gemcitabine and cisplatin as standard of care. Benefit of targeted molecular therapy is not clear at present, but ongoing research in genetic profiling of tumor may help to improve current clinical practice. Patients with cholangiocarcinoma have to be discussed during multidisciplinary team meetings.

Research paper thumbnail of Abstract 2680: Characterization of cultured circulating melanoma cells for individualized therapy

Cancer Research, 2012

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented st... more Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented study circulating melanoma cells (CMSs) have been isolated from a B16 mouse melanoma model and from melanoma patients. Culture of CMCs has enabled us to perform drug sensitivity testing on them. The main hypothesis was to compare drug sensitivity of primary tumor cells grown in culture with the sensitivity of CMCs grown in vitro after capture. B16 murine melanoma was implanted i.p. in mice. Fourteen days after peritoneal injection, mice were sacrificed and blood obtained by cardiac puncture for CMCs-capture. The CMCs were captured either by immunomagnetic separation (Adnagen, AdnaTest Melanoma Select™, Germany) and/or blood added to culture flask containing growing medium. Sensitivity to cisplatin (CDDP) was tested with the MTT-assay at different concentrations. Primary melanoma and disseminated tumor cells (DTCs) were also cultured and tested. The DTCs were isolated from peritoneal washing (ascites like cells), lymph nodes and peritoneal fat tissue. CMCs and DTCs were found less sensitive to CDDP than primary melanoma cells. The ability to capture and culture CTCs enabled these studies which can be used for individualized therapy design. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2680. doi:1538-7445.AM2012-2680

Research paper thumbnail of Dosažení kompletní remise při kombinaci FOLFIRI + aflibercept (Zaltrap®) u pacientky s karcinomem kolon a s generalizací do jater a plic

Research paper thumbnail of In vitro culturing of circulating tumor cells in lung cancer management

Research paper thumbnail of Abstract 5123: In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models

Research paper thumbnail of In vitro culturing of circulating tumor cells in lung cancer management

Research paper thumbnail of Essentials of circulating tumor cells for clinical research and practice

Critical Reviews in Oncology/Hematology, 2013

Research paper thumbnail of Circulating Tumor Cells, Circulating Tumor DNA and Other Blood-based Prognostic Scores in Pancreatic Ductal Adenocarcinoma – Mini-Review

Research paper thumbnail of Abstract 5123: In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models

Cancer Research, 2013

Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC In the present study ... more Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC In the present study circulating tumor cells (CTCs) were isolated from H460-RFP orthotopic human lung cancer model in mice. In vitro culture of CTCs has enabled us comparison of the chemosensitivity of tumor cells from primary H460 culture and of CTCs solated from blood grown in vitro. Cell line H460-RFP human lung cancer cells were grown to 70-80% confluence. Primary tumor were grown after subcutaneous injection of cultured cells. Fragments of tumor tissue (1 mm × 1 mm) were implanted into the lungs of nude mice. At 14 days, the mice were sacrificied and blood was taken by cardiac puncture for CTC-analysis. The blood (0.5 ml), obtained by cardiac puncture, was put into an EDTA tube (BD) and the CTC cells were enriched by immunomagnetic separation targeted to eptihelial antigens. The enriched fraction or whole blood was put into culture. Cell growth was then monitored by fluorescence microscopy. The isolation and culture of the fluorescent CTC will enable their further characterization. Citation Format: Katarina Kolostova, Marian Liberko, Eva Hroncova, Robert M. Hoffman, Vladimir Bobek. In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5123. doi:10.1158/1538-7445.AM2013-5123

Research paper thumbnail of Abstract 2680: Characterization of cultured circulating melanoma cells for individualized therapy

Cancer Research, 2012

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented st... more Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented study circulating melanoma cells (CMSs) have been isolated from a B16 mouse melanoma model and from melanoma patients. Culture of CMCs has enabled us to perform drug sensitivity testing on them. The main hypothesis was to compare drug sensitivity of primary tumor cells grown in culture with the sensitivity of CMCs grown in vitro after capture. B16 murine melanoma was implanted i.p. in mice. Fourteen days after peritoneal injection, mice were sacrificed and blood obtained by cardiac puncture for CMCs-capture. The CMCs were captured either by immunomagnetic separation (Adnagen, AdnaTest Melanoma Select™, Germany) and/or blood added to culture flask containing growing medium. Sensitivity to cisplatin (CDDP) was tested with the MTT-assay at different concentrations. Primary melanoma and disseminated tumor cells (DTCs) were also cultured and tested. The DTCs were isolated from peritoneal washing (ascites like cells), lymph nodes and peritoneal fat tissue. CMCs and DTCs were found less sensitive to CDDP than primary melanoma cells. The ability to capture and culture CTCs enabled these studies which can be used for individualized therapy design. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2680. doi:1538-7445.AM2012-2680

Research paper thumbnail of Complete remission and long-term response to gemcitabine + nab-paclitaxel in patient with metastatic pancreatic adenocarcinoma

Research paper thumbnail of The current role of radiotherapy and systemic therapy in the multidisciplinary treatment of cholangiocarcinoma

Casopis lekaru ceskych, 2019

Cholangiocarcinoma is a cancer with very poor prognosis. The only potentially curative approach i... more Cholangiocarcinoma is a cancer with very poor prognosis. The only potentially curative approach is surgical resection of tumor. However, the rate of local and distant recurrence after radical surgery is still high. Benefit of adjuvant therapy is not clearly defined, nevertheless patients at high risk of recurrence are indicated to chemotherapy or chemoradiotherapy. Locally advanced, unresectable disease can also be treated with chemotherapy alone, or with her combination with radiotherapy. Required radiation doses are relatively high, therefore it is necessary to use highly conformal radiation therapy. Treatment of metastatic disease is currently based on systemic therapy, combination of gemcitabine and cisplatin as standard of care. Benefit of targeted molecular therapy is not clear at present, but ongoing research in genetic profiling of tumor may help to improve current clinical practice. Patients with cholangiocarcinoma have to be discussed during multidisciplinary team meetings.

Research paper thumbnail of Abstract 2680: Characterization of cultured circulating melanoma cells for individualized therapy

Cancer Research, 2012

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented st... more Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL In the presented study circulating melanoma cells (CMSs) have been isolated from a B16 mouse melanoma model and from melanoma patients. Culture of CMCs has enabled us to perform drug sensitivity testing on them. The main hypothesis was to compare drug sensitivity of primary tumor cells grown in culture with the sensitivity of CMCs grown in vitro after capture. B16 murine melanoma was implanted i.p. in mice. Fourteen days after peritoneal injection, mice were sacrificed and blood obtained by cardiac puncture for CMCs-capture. The CMCs were captured either by immunomagnetic separation (Adnagen, AdnaTest Melanoma Select™, Germany) and/or blood added to culture flask containing growing medium. Sensitivity to cisplatin (CDDP) was tested with the MTT-assay at different concentrations. Primary melanoma and disseminated tumor cells (DTCs) were also cultured and tested. The DTCs were isolated from peritoneal washing (ascites like cells), lymph nodes and peritoneal fat tissue. CMCs and DTCs were found less sensitive to CDDP than primary melanoma cells. The ability to capture and culture CTCs enabled these studies which can be used for individualized therapy design. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2680. doi:1538-7445.AM2012-2680

Research paper thumbnail of Dosažení kompletní remise při kombinaci FOLFIRI + aflibercept (Zaltrap®) u pacientky s karcinomem kolon a s generalizací do jater a plic

Research paper thumbnail of In vitro culturing of circulating tumor cells in lung cancer management

Research paper thumbnail of Abstract 5123: In vitro culture for characterization of circulating GFP-expressing tumor cells in orthotopic lung cancer models

Research paper thumbnail of In vitro culturing of circulating tumor cells in lung cancer management

Research paper thumbnail of Essentials of circulating tumor cells for clinical research and practice

Critical Reviews in Oncology/Hematology, 2013