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Papers by Mariane Costa

Brazilian Journal of Nephrology

Introduction: Obesity, diabetes, and hypertension are common risk factors for chronic kidney dise... more Introduction: Obesity, diabetes, and hypertension are common risk factors for chronic kidney disease (CKD). CKD arises due to many pathological insults, including inflammation and oxidative stress, which affect renal function and destroy nephrons. Rice bran (RB) is rich in vitamins and minerals, and contains signiﬁcant amount of antioxidants. The aim of this study was to evaluate the preventive effect of RB on renal disease risk factors. Methods: Male Wistar rats (±325 g) were divided into two experimental groups to received a high sugar-fat diet (HSF, n = 8) or high sugar-fat diet with rice bran (HSF + RB, n = 8) for 20 weeks. At the end, renal function, body composition, metabolic parameters, renal inflammatory and oxidative stress markers were analyzed. Results: RB prevented obesity [AI (HSF= 9.92 ± 1.19 vs HSF + RB= 6.62 ± 0.78)ENT#093;, insulin resistance [HOMA (HSF= 83 ± 8 vs. HSF + RB= 42 ± 11)ENT#093;, dyslipidemia [TG (HSF= 167 ± 41 vs. HSF + RB=92 ± 40)ENT#093;, inflammati...

Journal of Biochemical and Molecular Toxicology

Nonalcoholic steatohepatitis (NASH) is a pathological manifestation with a progressive incidence ... more Nonalcoholic steatohepatitis (NASH) is a pathological manifestation with a progressive incidence in response to the epidemic of hepatic steatosis caused primarily by excessive energy intake. The present study unravels affected biological processes and functions by the presence of NASH in rats using a label‐free quantitative proteomic strategy. NASH was induced by a Western high‐sugar and high‐fat diet for 20 weeks. The liver tissue was collected for histology and for a mass spectrometry‐based proteomic protocol. The NASH group showed severe lipidosis, hepatocyte ballooning, and the presence of collagen deposition. Among upregulated proteins in NASH perilipin‐2 (Plin‐2; F6QBA3; difference [diff]: 2.29), ferritin heavy (Fth1; Q66HI5; diff: 2.19) and light (Ftl1; P02793; diff: 1.75) chains, macrophage migration inhibitory factor 1 (Mif; P30904; diff: 1.69), and fibronectin (Fn1; F1LST1; diff: 0.35) were observed, whereas among downregulated proteins, plectin (Q6S399; diff: −3.34), some Cyp2 family proteins of the cytochrome P450 complex, glutathione S‐transferases, flavin‐containing monooxygenase 1 (Fmo1; P36365; diff: −2.08), acetyl‐CoA acetyltransferase 2 (Acat2; Q5XI22; diff: −2.25), acyl‐CoA oxidase 2 (Acox2; F1LNW3; diff: −1.59), and acyl‐CoA oxidase 3 (Acox3; F1M9A7; diff: −2.41) were observed. Also, biological processes and functions such as LPS/IL‐1 inhibition of RXR, fatty acid metabolism, Nrf2‐mediated oxidative stress response, xenobiotic metabolism, and PXR/RXR and CAR/RXR activations were predicted to be affected. In conclusion, the liver of rats with NASH induced by Western diet shows a decreased capacity of metabolizing lipids, fatty acids, and xenobiotic compounds that predispose fibrosis development.

Arquivos Brasileiros de Cardiologia, 2021

Fundamento: A obesidade é uma condição inflamatória crônica de baixo grau relacionada a distúrbio... more Fundamento: A obesidade é uma condição inflamatória crônica de baixo grau relacionada a distúrbios cardíacos. No entanto, o mecanismo responsável pela inflamação cardíaca relacionada à obesidade não é claro. O receptor do tipo toll 4 (TLR-4) pertence a um receptor da família das transmembranas, responsável pela resposta imune, cuja ativação estimula a produção de citocinas pró-inflamatórias. Objetivo: Testar se a ativação do receptor TLR-4 participa do processo de cardiomiopatia da obesidade, devido à produção de citocinas por meio da ativação do NF-ĸB. Métodos: Ratos Wistar machos foram randomizados em dois grupos: o grupo controle (C, n = 8 animais) que recebeu dieta padrão/água e o grupo obeso (OB, n = 8 animais) que foi alimentado com dieta rica em açúcar e gordura e água mais 25% de sacarose por 30 semanas. Análise nutricional: peso corporal, índice de adiposidade, alimentos, água e ingestão calórica. Análise de distúrbios relacionados à obesidade: glicose plasmática, ácido úrico e triglicerídeos, HOMA-IR, pressão arterial sistólica, TNF-α no tecido adiposo. A análise cardíaca incluiu: expressão das proteínas TLR-4 e NF-ĸB, níveis de TNF-α e IL-6. Comparação pelo teste t de Student não pareado ou teste de Mann-Whitney com um valor de p <0,05 como estatisticamente significativo. Resultados: O grupo OB apresentou obesidade, glicose elevada, triglicerídeos, ácido úrico, HOMA, pressão arterial sistólica e TNF-α no tecido adiposo. O grupo OB apresentou remodelação cardíaca e disfunção diastólica. A expressão de TLR-4 e NF-ĸB e os níveis de citocinas foram maiores em OB. Conclusão: Nossos achados concluem que, em uma condição obesogênica, a inflamação derivada da ativação do TLR-4 cardíaco pode ser um mecanismo capaz de levar à remodelação e disfunção cardíaca.

Pathology, 2020

Abstract Diabetes mellitus is a complex disease characterized by glucose, lipid, and protein dere... more Abstract Diabetes mellitus is a complex disease characterized by glucose, lipid, and protein deregulated metabolism due to insulin deprivation affecting various tissues in the human body. Dysmetabolism in diabetes could lead to oxidative stress, which, in turn, could contribute to pathological processes in the diabetic condition. Natural compounds have been recognized as potential natural sources for diabetes prevention, management, and treatment. Yacon (Smallanthus sonchifolius), belonging to the Asteraceae family, is an indigean perennial herbaceous plant native to the Andes mountains, and is rich in polyphenols and bioactive compounds. Several studies have attributed antioxidant and antidiabetogenic effects to yacon. For these reasons, this chapter aims to elucidate the effects of yacon on diabetic-related complications based on its phytochemical profile. First, the diabetes condition and how antioxidants, in general terms, could act are described. Further, yacon is described in its most important approaches and, finally, its phytochemical profile and interactions on diabetes are discussed.

Cardiovascular disease is a term for disorders of the heart and blood vessels and its cause is mu... more Cardiovascular disease is a term for disorders of the heart and blood vessels and its cause is multifactorial, involving behavioral, environmental, and genetic factors. All these causes can be associated with a phenomenon called oxidative stress. In this condition, oxidative stress occurs mainly due to the overproduction of reactive species that can damage macromolecules and can act as intracellular signaling. Several cellular compartments and systems are involved with the production of reactive species; however, mitochondria represent the largest source of reactive species in the cardiovascular system. The mechanisms by which oxidative stress can act, leading to the development of diseases, is still controversial. Thus this chapter aims to elucidate some of these mechanisms. We will present the conditions of ischemia and reperfusion, atherosclerosis, hyperlipidemia, inflammation, and peripheral arterial hypertension.

Molecular and Cellular Endocrinology, 2021

The literature has reported a higher prevalence of negative clinical outcomes due to Coronavirus ... more The literature has reported a higher prevalence of negative clinical outcomes due to Coronavirus disease 19 (COVID-19) in obese individuals. This can be explained by the cytokine storm, result from the cytokine production from both obesity and viral infection. Gamma-oryzanol (γOz) is a compound with anti-inflammatory and antioxidant activities. However, little is known about the γOz action as a possible agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). The aim of this study was to test the hypothesis that γOz attenuates the cytokine storm by stimulating PPAR-γ in the adipose tissue. Methods Male Wistar rats were randomly divided into three experimental groups and fed ad libitum for 30 weeks with control diet (C, n = 6), high sugar- fat diet (HSF, n = 6) or high sugar- fat diet + γOz (HSF + γOz, n = 6). HSF groups also received water + sucrose (25%). The γOz dose was 0.5% in the chow. Evaluation in animals included caloric intake, body weight, adiposity index, plasma triglycerides, and HOMA-IR. In adipose tissue was evaluated: PPAR-γ gene and protein expression, inflammatory and oxidative stress parameters, and histological analysis. Results Adipose tissue dysfunction was observed in HSF group, which presented remarkable PPAR-γ underexpression and increased levels of cytokines, other inflammatory markers and oxidative stress. The γOz treatment prevented adipose tissue dysfunction and promoted PPAR-γ overexpression. Conclusion Natural compounds as γOz can be considered a coadjutant therapy to prevent the cytokine storm in COVID-19 patients with obesity conditions.

European Journal of Nutrition, 2021

Purpose This study aimed to evaluate the effect of rice bran (RB) supplementation to a high-sugar... more Purpose This study aimed to evaluate the effect of rice bran (RB) supplementation to a high-sugar fat (HSF) diet on cardiac dysfunction in an experimental obesity model. Methods Male Wistar rats were distributed into three groups: control, high-sugar fat, and high-sugar fat supplemented with 11% RB for 20 weeks. Results HSF diet promoted obesity and metabolic complications. Obese rats showed cardiac structural and functional impairment associated with high levels of interleukin-6, tumoral necrosis factor alpha, and malondialdehyde, and decreased activity of superoxide dismutase and catalase in the myocardium. RB supplementation was able to mitigate obesity and its metabolic alterations in HSF diet-fed animals. Moreover, the RB also prevented structural and functional damage, inflammation, and redox imbalance in the heart of these animals. Conclusion This study suggests that RB supplementation prevents cardiac dysfunction in rats fed on HSF by modulating systemic metabolic complicati...

Nutrire, 2020

The consumption of products containing fructose is associated with metabolic disorders and nonalc... more The consumption of products containing fructose is associated with metabolic disorders and nonalcoholic fatty liver disease (NAFLD). Several pathways have been described associating the fructose intake and liver damage, among them the reduced peroxisome proliferator-activated receptor alpha (PPAR-α) expression, insulin resistance, inflammation, and oxidative stress. Curcuma longa L. is a member of the Zingiberaceae family and has traditionally been used as a medicinal herb. The aim was to verify the therapeutic effect of Curcuma longa L. in the physiopathology of NAFLD due to the fructose consumption. During 16 weeks, animals (n = 12) received standard chow + fructose in drinking water (30%) ad libitum. After, animals were randomly allocated to receive placebo treatment (fructose, n = 6) or Curcuma longa L. treatment (fructose + Cl, n = 6) for further 8 weeks. Both groups continued receiving standard chow + fructose in drinking water (30%) ad libitum. Curcuma longa L. was effective to reduce insulin resistance and triglycerides levels, increase PPAR-α protein expression in liver, and attenuate macrovesicular steatosis, liver IL-6 levels, and oxidative stress (with lower protein carbonylation, MDA, and DNA damage and increase in SOD and catalase antioxidant enzymes) induced by fructose consumption. Curcuma longa L. treatment was effective to modulate the physiopathology of NAFLD due to fructose consumption.

Oxidative Medicine and Cellular Longevity, 2020

Solanum paniculatum L. or popularly known as “jurubeba” is an herbal medicinal plant. A few studi... more Solanum paniculatum L. or popularly known as “jurubeba” is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg...

Antioxidants, 2019

Background: The higher consumption of fat and sugar are associated with obesity development and i... more Background: The higher consumption of fat and sugar are associated with obesity development and its related diseases such as non-alcoholic fatty liver disease (NAFLD). Lycopene is an antioxidant whose protective potential on fatty liver degeneration has been investigated. The aim of this study was to present the therapeutic effects of lycopene on NAFLD related to the obesity induced by a hypercaloric diet. Methods: Wistar rats were distributed in two groups: Control (Co, n = 12) and hypercaloric (Ob, n = 12). After 20 weeks, the animals were redistributed into the control group (Co, n = 6), control group supplemented with lycopene (Co+Ly, n = 6), obese group (Ob, n = 6), and obese group supplemented with lycopene (Ob+Ly, n = 6). Ob groups also received water + sucrose (25%). Animals received lycopene solution (10 mg/kg/day) or vehicle (corn oil) via gavage for 10 weeks. Results: Animals which consumed the hypercaloric diet had higher adiposity index, increased fasting blood glucose,...

International Journal of Molecular Sciences, 2019

The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) is one of the most im... more The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) is one of the most important oxidative stress regulator in the human body. Once Nrf2 regulates the expression of a large number of cytoprotective genes, it plays a crucial role in the prevention of several diseases, including age-related disorders. However, the involvement of Nrf2 on these conditions is complex and needs to be clarified. Here, a brief compilation of the Nrf2 enrollment in the pathophysiology of the most common age-related diseases and bring insights for future research on the Nrf2 pathway is described. This review shows a controversial response of this transcriptional factor on the presented diseases. This reinforces the necessity of more studies to investigate modulation strategies for Nrf2, making it a possible therapeutic target in the treatment of age-related disorders.

Food and Nutrition Sciences, 2019

Background: The modern dietary habit, which is rich in refined carbohydrates and saturated fats, ... more Background: The modern dietary habit, which is rich in refined carbohydrates and saturated fats, increases the risk of chronic diseases due to the proinflammatory effect of these nutrients. Aim: To evaluate the impact of high sugar-fat diet in the development of metabolic-inflammatory disorders in non-obese animals. Methods: Male Wistar rats were distributed into two groups according to the diet: control and high sugar-fat for 30 weeks. It was analyzed: dietary efficiency; chow, water and caloric intake; metabolic and hormonal profile in plasma and inflammatory cytokines in epididymal adipose tissue. Data were compared by Student's t test or by Mann-Whitney U test with p < 0.05 as significant. Results: HSF presented lower chow intake, higher water consumption and dietary efficiency with no difference in the caloric intake. The final body weight (FBW) and weight gain (WG) were lower in the HSF group and there was no difference in the adiposity index (AI). HSF diet-induced hyperglycemia and hyperinsulinemia with no difference for Homeostatic Model Assessment for Insulin Resistance (HOMA-IR). Triglycerides, uric acid, adiponectin and leptin levels were higher in the HSF group. The HSF group showed increased interleukin-6 (IL-6) and tumoral necrosis factor-alpha (TNF-α) levels in epidydimal adipose tissue. The urinary protein-creatinine ratio and albuminuria were higher in the HSF group. Conclusion: HSF diet intake is directly involved in the development of metabolic-inflammatory disorders independent of obesity, dissociating the view that increased adiposity is the major risk factor for complications commonly found in obese individuals.

XXI I Congresso Brasileiro de Nutrologia, 2018

Brazilian Journal of Development, 2020

The primary and unhealthy conditions experienced by recyclable material collectors, both professi... more The primary and unhealthy conditions experienced by recyclable material collectors, both professionally and socially, stem from, among other aspects, the lack of implementation of public policies aimed at solid waste management, the scarce development of technologies that optimize the stages of management solid waste management and the professional practice of these workers. The main objective of this work was to develop and evaluate social technologies for the management of dry recyclable solid waste and risk prevention in the professional practice of recyclable material collectors. The work carried out from March 2014 to March 2019, in Campina Grande, state of Paraíba, Brazil, followed the principles of participatory and experimental research and involved recyclable material collectors organized in associations and families that joined the selective collection. Four models of collectors were developed and evaluated for the storage of recyclable solid waste inside and outside homes;