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Papers by Marianne Philippe
The European respiratory journal, Apr 1, 1998
Journal of physics, May 22, 2015
Poor bed allocation to each specialty might lead to a surplus of available beds during weekends. ... more Poor bed allocation to each specialty might lead to a surplus of available beds during weekends. The introduction of a "week unit" that is only available during working days may improve the efficiency of bed allocation by uniting the patient flow of two regular units, before converting the regular beds of one unit into week beds. In order to test the possible introduction of this system in three services of the Saint-Luc hospital, we created a simulation model that considers new allocation rules to direct the patients into the right unit.
Journal of Internal Medicine, 1997
Objectives. To assess the prevalence of activated protein C resistance (APC-R) among healthy subj... more Objectives. To assess the prevalence of activated protein C resistance (APC-R) among healthy subjects and thromboembolic patients and to determine the clinical characteristics associated with APC-R. Design. A prospective study. Setting. One academic medical centre. Subjects. 91 health controls and 126 thromboembolic patients. Measurements. Patients and control were genotyped for the factor V Leiden (VaQ506) mutation. The anticoagulant response of the patient's plasma to activated protein C was also determined. Results. The frequency of APC-R was 3n3 % among healthy control subjects and 22 % among thrombotic
PubMed, Nov 1, 1990
Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about ... more Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about the involvement of the BCR and C-ABL genes are available. We report here the case of a 64-year-old woman presenting with a typical multiple myeloma and a complex Philadelphia (Ph) chromosome that we investigated at a molecular level using conventional DNA techniques and the polymerase chain reaction (PCR). No rearrangement was observed within the major breakpoint cluster region (M-BCR) although she was found to have a P190 BCR/ABL hybrid transcript using PCR. As far as we know, this is the first description of a P190-type mRNA in a patient with a chronic lymphoid disorder. Since P190 is almost always associated in man with acute forms of hematological malignancies, this suggests that other factors may play a role in determining the phenotype of the disease.
Archives of Biochemistry and Biophysics, 1995
Acta Clinica Belgica (Multilingual Edition), 2010
Clinical Chemistry, 1995
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have e... more In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results.
Hemoglobin, 1993
Nine asymptomatic members of a family of Belgian origin, spanning three generations, present typi... more Nine asymptomatic members of a family of Belgian origin, spanning three generations, present typical features of heterozygous beta-thalassemia. Since no mutation was detected with a large panel of oligonucleotide probes, the thalassemia gene was investigated by direct sequencing of DNA segments amplified by the polymerase chain reaction. A T-->C transition was detected in the translation initiation codon (ATG). The mutation, which abolishes an Nco I restriction site, was further confirmed by enzymatic digestion as well as by dot-blot hybridization of the amplified products with allele-specific oligonucleotide probes. It produced a beta zero-thalassemia phenotype characterized by marked microcytosis and hypochromia, as well as by an in vitro beta/alpha chain synthesis ratio close to O.5. Search for haplotype linkage showed the mutation to be associated with haplotype IX [- + - + + + +].
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have e... more In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results
Nous rapportons l'observation d'une evolution inhabituelle d'un patient diabetique âg... more Nous rapportons l'observation d'une evolution inhabituelle d'un patient diabetique âge de 76 ans admis pour neuropathie des membres inferieurs et superieurs. Il existait entre autres une impotence fonctionnelle majeure avec ataxie et amyotrophie musculaire. L'ensemble des elements cliniques et l'eventail des examens complementaires (electromyographie, resonance magnetique du plexus, ponction lombaire) vont mener au diagnostic de polyneuropathie chronique inflammatoire demyelinisante.
Diabetes & Metabolism, 2009
Blood, 1993
We sequentially studied bone marrow (BM) samples of 25 patients in complete remission of an acute... more We sequentially studied bone marrow (BM) samples of 25 patients in complete remission of an acute lymphoblastic leukemia (ALL) using a simplified polymerase chain reaction (PCR) strategy (direct use of the PCR product as a clonogenic probe recognizing rearranged Ig heavy chain sequences) as a first approach. BM aspirates were serially investigated after obtention of a complete response. When sensitivity was less than 1:10(4), the PCR fragment was sequenced and a specific oligonucleotide was synthetized and used as a probe (five cases). Cases in which minimal residual disease (MRD) became undetectable were cross- controlled using either TCR delta rearrangement or a specific translocation to circumvent the problem of false-negative results arising from clonal evolution. The median follow-up was 30 months (3 to 51 months). Within the first 3 months of complete remission, MRD was detectable in 22 of 23 investigated patients and remained so in 19 of 21 patients examined at 6 months, rega...
Nouvelle revue française d'hématologie, 1993
Thymoma is generally considered as an epithelial malignancy surrounded by lymphocytes not belongi... more Thymoma is generally considered as an epithelial malignancy surrounded by lymphocytes not belonging to the tumour. This report documents a case of malignant thymoma associated with a lymphocytic pleural effusion. The pleural lymphocytes were mature T-cells, a small proportion of which were shown to have a monoclonal rearrangement of the beta-chain of the T-cell receptor. On the contrary, the lymphocytes of peripheral blood exhibited a germline configuration. The nature of the monoclonal population found in the pleural fluid is discussed and if the cells are of thymic origin their clonal configuration may point to a neoplastic nature of lymphocytes infiltrating malignant thymoma. Alternatively, the clonal T-cell subpopulation may result from an aberrant immunological response to the thymoma.
The European respiratory journal, Apr 1, 1998
Journal of physics, May 22, 2015
Poor bed allocation to each specialty might lead to a surplus of available beds during weekends. ... more Poor bed allocation to each specialty might lead to a surplus of available beds during weekends. The introduction of a "week unit" that is only available during working days may improve the efficiency of bed allocation by uniting the patient flow of two regular units, before converting the regular beds of one unit into week beds. In order to test the possible introduction of this system in three services of the Saint-Luc hospital, we created a simulation model that considers new allocation rules to direct the patients into the right unit.
Journal of Internal Medicine, 1997
Objectives. To assess the prevalence of activated protein C resistance (APC-R) among healthy subj... more Objectives. To assess the prevalence of activated protein C resistance (APC-R) among healthy subjects and thromboembolic patients and to determine the clinical characteristics associated with APC-R. Design. A prospective study. Setting. One academic medical centre. Subjects. 91 health controls and 126 thromboembolic patients. Measurements. Patients and control were genotyped for the factor V Leiden (VaQ506) mutation. The anticoagulant response of the patient's plasma to activated protein C was also determined. Results. The frequency of APC-R was 3n3 % among healthy control subjects and 22 % among thrombotic
PubMed, Nov 1, 1990
Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about ... more Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about the involvement of the BCR and C-ABL genes are available. We report here the case of a 64-year-old woman presenting with a typical multiple myeloma and a complex Philadelphia (Ph) chromosome that we investigated at a molecular level using conventional DNA techniques and the polymerase chain reaction (PCR). No rearrangement was observed within the major breakpoint cluster region (M-BCR) although she was found to have a P190 BCR/ABL hybrid transcript using PCR. As far as we know, this is the first description of a P190-type mRNA in a patient with a chronic lymphoid disorder. Since P190 is almost always associated in man with acute forms of hematological malignancies, this suggests that other factors may play a role in determining the phenotype of the disease.
Archives of Biochemistry and Biophysics, 1995
Acta Clinica Belgica (Multilingual Edition), 2010
Clinical Chemistry, 1995
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have e... more In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results.
Hemoglobin, 1993
Nine asymptomatic members of a family of Belgian origin, spanning three generations, present typi... more Nine asymptomatic members of a family of Belgian origin, spanning three generations, present typical features of heterozygous beta-thalassemia. Since no mutation was detected with a large panel of oligonucleotide probes, the thalassemia gene was investigated by direct sequencing of DNA segments amplified by the polymerase chain reaction. A T-->C transition was detected in the translation initiation codon (ATG). The mutation, which abolishes an Nco I restriction site, was further confirmed by enzymatic digestion as well as by dot-blot hybridization of the amplified products with allele-specific oligonucleotide probes. It produced a beta zero-thalassemia phenotype characterized by marked microcytosis and hypochromia, as well as by an in vitro beta/alpha chain synthesis ratio close to O.5. Search for haplotype linkage showed the mutation to be associated with haplotype IX [- + - + + + +].
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have e... more In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results
Nous rapportons l'observation d'une evolution inhabituelle d'un patient diabetique âg... more Nous rapportons l'observation d'une evolution inhabituelle d'un patient diabetique âge de 76 ans admis pour neuropathie des membres inferieurs et superieurs. Il existait entre autres une impotence fonctionnelle majeure avec ataxie et amyotrophie musculaire. L'ensemble des elements cliniques et l'eventail des examens complementaires (electromyographie, resonance magnetique du plexus, ponction lombaire) vont mener au diagnostic de polyneuropathie chronique inflammatoire demyelinisante.
Diabetes & Metabolism, 2009
Blood, 1993
We sequentially studied bone marrow (BM) samples of 25 patients in complete remission of an acute... more We sequentially studied bone marrow (BM) samples of 25 patients in complete remission of an acute lymphoblastic leukemia (ALL) using a simplified polymerase chain reaction (PCR) strategy (direct use of the PCR product as a clonogenic probe recognizing rearranged Ig heavy chain sequences) as a first approach. BM aspirates were serially investigated after obtention of a complete response. When sensitivity was less than 1:10(4), the PCR fragment was sequenced and a specific oligonucleotide was synthetized and used as a probe (five cases). Cases in which minimal residual disease (MRD) became undetectable were cross- controlled using either TCR delta rearrangement or a specific translocation to circumvent the problem of false-negative results arising from clonal evolution. The median follow-up was 30 months (3 to 51 months). Within the first 3 months of complete remission, MRD was detectable in 22 of 23 investigated patients and remained so in 19 of 21 patients examined at 6 months, rega...
Nouvelle revue française d'hématologie, 1993
Thymoma is generally considered as an epithelial malignancy surrounded by lymphocytes not belongi... more Thymoma is generally considered as an epithelial malignancy surrounded by lymphocytes not belonging to the tumour. This report documents a case of malignant thymoma associated with a lymphocytic pleural effusion. The pleural lymphocytes were mature T-cells, a small proportion of which were shown to have a monoclonal rearrangement of the beta-chain of the T-cell receptor. On the contrary, the lymphocytes of peripheral blood exhibited a germline configuration. The nature of the monoclonal population found in the pleural fluid is discussed and if the cells are of thymic origin their clonal configuration may point to a neoplastic nature of lymphocytes infiltrating malignant thymoma. Alternatively, the clonal T-cell subpopulation may result from an aberrant immunological response to the thymoma.