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Papers by Marianne Skeie

Research paper thumbnail of Growth and toxin profiles of Bacillus cereus isolated from different food sources

International Journal of Food Microbiology, Sep 28, 2001

Research paper thumbnail of Growth and toxin profiles of Bacillus cereus isolated from different food sources

International Journal of Food Microbiology, 2001

Research paper thumbnail of Comparative studies of class IIa bacteriocins of lactic acid bacteria

Applied and …, 1998

Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified... more Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and ...

Research paper thumbnail of Comparative studies of class IIa bacteriocins of lactic acid bacteria

Applied and …, 1998

Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified... more Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and ...

Research paper thumbnail of Characterization of the Bacillus cereus Nhe enterotoxin

Research paper thumbnail of Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2

Molecular cancer research : MCR, 2009

Overexpression of the epidermal growth factor receptor (EGFR), ErbB2, and ErbB3 promotes growth a... more Overexpression of the epidermal growth factor receptor (EGFR), ErbB2, and ErbB3 promotes growth and antiapoptotic signaling. Overexpression of ErbB2 in breast cancer is associated with poor clinical outcome, and ways of down-regulating ErbB2 are important as therapeutic approaches. In contrast to EGFR, ErbB2 has been shown to be endocytosis deficient. However, down-regulation of ErbB2 can be induced by incubation of cells with geldanamycin and geldanamycin derivatives, counteracting the stabilizing function of heat shock protein 90 on ErbB2. In the present study, we have made use of stably transfected isogenic cell lines expressing ErbB2 only or ErbB2 together with EGFR and/or ErbB3. We now show that whereas ErbB2 can be down-regulated by incubation with geldanamycin in cells expressing ErbB2 only, the rate of geldanamycin-induced down-regulation increases significantly when the cells additionally express EGFR and/or ErbB3. This increase does, however, not correlate with activation/...

Research paper thumbnail of Epsin 1 is Involved in Recruitment of Ubiquitinated EGF Receptors into Clathrin-Coated Pits

Research paper thumbnail of A Chimeric Pre-ubiquitinated EGF Receptor is Constitutively Endocytosed in a Clathrin-Dependent, but Kinase-Independent Manner

Research paper thumbnail of Pertuzumab increases epidermal growth factor receptor down-regulation by counteracting epidermal growth factor receptor-ErbB2 heterodimerization

Molecular Cancer Therapeutics, 2009

Research paper thumbnail of Additional Information

Research paper thumbnail of Preubiquitinated chimeric ErbB2 is constitutively endocytosed and subsequently degraded in lysosomes

Experimental Cell Research, 2013

Research paper thumbnail of Dysregulation of Ack1 inhibits down-regulation of the EGF receptor

Experimental Cell Research, 2008

The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also bee... more The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack1 co-localizes with the EGFR upon EGF-induced endocytosis in cells with moderate over-expression of Ack. This co-localization is mainly observed in early endosomes. Furthermore, we found that over-expression of Ack1 retained the EGFR at the limiting membrane of early endosomes, inhibiting sorting to inner vesicles of multivesicular bodies. Down-regulation of Ack1 in HeLa cells resulted in reduced rate of (125)I-EGF internalization, whereas internalization of (125)I-transferrin was not affected. In cells where Ack1 had been knocked down by siRNA, recycling of internalized (125)I-EGF was increased, while degradation of (125)I-EGF was inhibited. Together, these data suggest that Ack1 is involved in an early step of EGFR desensitization.

Research paper thumbnail of Expression of Epidermal Growth Factor Receptor in Laryngeal Dysplasia and Carcinoma

Acta Oto-laryngologica, 1990

Research paper thumbnail of Pertuzumab Increases 17-AAG-Induced Degradation of ErbB2, and This Effect Is Further Increased by Combining Pertuzumab with Trastuzumab

Research paper thumbnail of Growth and toxin profiles of Bacillus cereus isolated from different food sources

International Journal of Food Microbiology, Sep 28, 2001

Research paper thumbnail of Growth and toxin profiles of Bacillus cereus isolated from different food sources

International Journal of Food Microbiology, 2001

Research paper thumbnail of Comparative studies of class IIa bacteriocins of lactic acid bacteria

Applied and …, 1998

Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified... more Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and ...

Research paper thumbnail of Comparative studies of class IIa bacteriocins of lactic acid bacteria

Applied and …, 1998

Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified... more Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and ...

Research paper thumbnail of Characterization of the Bacillus cereus Nhe enterotoxin

Research paper thumbnail of Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2

Molecular cancer research : MCR, 2009

Overexpression of the epidermal growth factor receptor (EGFR), ErbB2, and ErbB3 promotes growth a... more Overexpression of the epidermal growth factor receptor (EGFR), ErbB2, and ErbB3 promotes growth and antiapoptotic signaling. Overexpression of ErbB2 in breast cancer is associated with poor clinical outcome, and ways of down-regulating ErbB2 are important as therapeutic approaches. In contrast to EGFR, ErbB2 has been shown to be endocytosis deficient. However, down-regulation of ErbB2 can be induced by incubation of cells with geldanamycin and geldanamycin derivatives, counteracting the stabilizing function of heat shock protein 90 on ErbB2. In the present study, we have made use of stably transfected isogenic cell lines expressing ErbB2 only or ErbB2 together with EGFR and/or ErbB3. We now show that whereas ErbB2 can be down-regulated by incubation with geldanamycin in cells expressing ErbB2 only, the rate of geldanamycin-induced down-regulation increases significantly when the cells additionally express EGFR and/or ErbB3. This increase does, however, not correlate with activation/...

Research paper thumbnail of Epsin 1 is Involved in Recruitment of Ubiquitinated EGF Receptors into Clathrin-Coated Pits

Research paper thumbnail of A Chimeric Pre-ubiquitinated EGF Receptor is Constitutively Endocytosed in a Clathrin-Dependent, but Kinase-Independent Manner

Research paper thumbnail of Pertuzumab increases epidermal growth factor receptor down-regulation by counteracting epidermal growth factor receptor-ErbB2 heterodimerization

Molecular Cancer Therapeutics, 2009

Research paper thumbnail of Additional Information

Research paper thumbnail of Preubiquitinated chimeric ErbB2 is constitutively endocytosed and subsequently degraded in lysosomes

Experimental Cell Research, 2013

Research paper thumbnail of Dysregulation of Ack1 inhibits down-regulation of the EGF receptor

Experimental Cell Research, 2008

The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also bee... more The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack1 co-localizes with the EGFR upon EGF-induced endocytosis in cells with moderate over-expression of Ack. This co-localization is mainly observed in early endosomes. Furthermore, we found that over-expression of Ack1 retained the EGFR at the limiting membrane of early endosomes, inhibiting sorting to inner vesicles of multivesicular bodies. Down-regulation of Ack1 in HeLa cells resulted in reduced rate of (125)I-EGF internalization, whereas internalization of (125)I-transferrin was not affected. In cells where Ack1 had been knocked down by siRNA, recycling of internalized (125)I-EGF was increased, while degradation of (125)I-EGF was inhibited. Together, these data suggest that Ack1 is involved in an early step of EGFR desensitization.

Research paper thumbnail of Expression of Epidermal Growth Factor Receptor in Laryngeal Dysplasia and Carcinoma

Acta Oto-laryngologica, 1990

Research paper thumbnail of Pertuzumab Increases 17-AAG-Induced Degradation of ErbB2, and This Effect Is Further Increased by Combining Pertuzumab with Trastuzumab

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