Marie KOFFI - Academia.edu (original) (raw)
Papers by Marie KOFFI
International Journal of Plant & Soil Science, 2021
Yam, Dioscorea spp. (L) is an important foodstuff that plays a key role in the agricultural syste... more Yam, Dioscorea spp. (L) is an important foodstuff that plays a key role in the agricultural system in Côte d'Ivoire. This plant is however subject to several diseases during its cultivation and conservation. The losses caused by rots of tubers in storage constitute a major risk for economic profitability and for the food safety of consumers. The development of an effective and environmental friendly control method has been initiated. The objective of this work is to improve the conservation of Discorea alata tubers through the use of aqueous extracts of Ocimum gratissimum and Chromolaena odorata. To do this, three different doses (33 g/l; 39 g/l and 50 g/l) of aqueous extracts of Ocimum gratissimum and Chromolaena odorata were applied to yam slices before inoculation with Colletotrichum sp. Results showed that applying different doses of aqueous plant extract to yam slices before inoculation of the fungal strain caused less rot. The aqueous extracts of the two plants showed anti...
Today, one major practice to control diseases and pests in banana fields is the use of in vitro p... more Today, one major practice to control diseases and pests in banana fields is the use of in vitro plantlets. However, nematodes, in particular Radopholus similis, remain a major problem. The restriction on the use of nematicides has encouraged the development of alternative strategies, among which the use of biological control organisms (BCO) such as arbuscular mycorrhizal fungi (AMF). Their beneficial effects on the control of nematodes have been demonstrated but their application in the field remains rare. In the recent decade the association of BCO to plants at the in vitro stage, has been proposed to produce plants more resistant to pathogens and to transplant stresses. The in vitro association of AMF to banana plantlets may thus represent an adequate system to investigate the interaction with nematodes and for the mass-production of mycorrhized plantlets. Here we developed an in vitro culture system for the mycorrhization of banana plantlets. It was used to study the interactions...
RESUME Le Niébé (Vigna unguiculata) est une légumineuse alimentaire qui fournit plus de la moitié... more RESUME Le Niébé (Vigna unguiculata) est une légumineuse alimentaire qui fournit plus de la moitié des protéines consommés. Cependant, sa culture est sujette à l'attaque de nombreux pathogènes et les rendements restent faibles. Une des alternatives pour un meilleur rendement est l'utilisation des produits agrochimiques. L'objectif recherché dans cette étude est d'optimiser la production de Vigna unguiculata. Pour atteindre cet objectif, des expérimentations ont été entreprises pour évaluer l'effet de l'utilisation du Furadan, du Mancomax et du Forum sur la nodulation lors de la culture de deux accessions (NbnK et NnB) de niébé. Pour ce faire, le sol de culture provenant d'une jachère de 2 ans au sein de l'université a été traité avec le Furadan 3 jours avant le semis tandis qu'un traitement foliaire a été effectué avec le Mancomax, et Forum à la dose du fabricant après la levée des plants de 5 jours. Un second traitement a été fait trois semaines après le semis. Les résultats montrent que les trois fongicides testés réduisent significativement de 50 à 84% le nombre de nodules des deux variétés et les nodules de l'accession NnB sont plus réduits que celles de l'accession NbnK.
Brown stem rot (BSR) is one of the most important diseases of soybean (Glycine max (L.) Merr.) in... more Brown stem rot (BSR) is one of the most important diseases of soybean (Glycine max (L.) Merr.) in the north-central soybean-production region (10), yet the causal agent of the disease is poorly known. The disease is characterized by a browning of the vascular system and pith, and characteristic chlorosis and necrosis of the foliage develops late in the season, usually in August (2). However, similar vascular discoloration is found late in the season with other diseases of soybean. Some BSR-resistant germ plasm is available (3,24), mostly identified by the extent of vascular browning, but development of resistant cultivars and deployment of resistance have been hampered by imprecise resistance rating, a poor understanding of the causal agent of the disease, and uncertainty about the variation in aggressiveness in the pathogen (16,24,29,33). The pathogen was described as Cephalosporium gregatum in 1948 by Allington and Chamberlain (2). Unfortunately, the fungus forms rather nondescript conidiophores, phialides, and conidia, and many unrelated species are morphologically similar. Many Cephalosporium spp. are now placed in the heterogeneous genus Acremonium, but the proper generic placement of C. gregatum is still in question. Gams (14) transferred the species to the genus Phialophora, though the fungus generally produces non-pigmented conidiophores. Sequences of ribosomal DNA suggest that Phialophora gregata is an anamorph of a discomycete (T. C. Harrington, unpublished). Two or more Acremonium-type fungi that are similar to P. gregata have been associated with brown stem rot in soybean and adzuki bean (5,18,23), and at least one of these Acremonium spp. causes vascular discoloration in soybean (23). Gray (16) reported that the disease in soybean is caused by either of two forms of P. gregata, a defoliating form (type I) and a nondefoliating form (type II), with the latter causing vascular discoloration but no foliar symptoms. Gray and Hepburn (18) did not
In Vitro Cellular & Developmental Biology - Plant
Micropropagated banana plantlets represent a good planting material to establish new growing area... more Micropropagated banana plantlets represent a good planting material to establish new growing areas or replace old plantations. The plantlets are devoid of pathogens but also of beneficial root inhabitants (e.g., endophytes and arbuscular mycorrhizal fungi [AMF]) that may help plants withstand stresses. Mycorrhization of banana is usually conducted at the hardening/post-hardening phases. Only a few studies have reported their application in vitro, and none have investigated the subsequent impact on ex vitro acclimatization. Here, we report for the first time the effects of in vitro mycorrhization of banana plantlets on growth following transfer to soil. Banana plantlets were pre-mycorrhized under autotrophic conditions. During in vitro culture, heavy root colonization of the banana plantlets was observed. At 5 and 7 wk after transfer to a peat-sand soil substrate, the root system remained highly colonized. The growth (pseudostem height) and biomass were significantly higher than thos...
Études internationales, 1997
Tous droits réservés © Études internationales, 1997 Ce document est protégé par la loi sur le dro... more Tous droits réservés © Études internationales, 1997 Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y compris la reproduction) est assujettie à sa politique d'utilisation que vous pouvez consulter en ligne.
International Journal of Plant & Soil Science, 2021
Yam, Dioscorea spp. (L) is an important foodstuff that plays a key role in the agricultural syste... more Yam, Dioscorea spp. (L) is an important foodstuff that plays a key role in the agricultural system in Côte d'Ivoire. This plant is however subject to several diseases during its cultivation and conservation. The losses caused by rots of tubers in storage constitute a major risk for economic profitability and for the food safety of consumers. The development of an effective and environmental friendly control method has been initiated. The objective of this work is to improve the conservation of Discorea alata tubers through the use of aqueous extracts of Ocimum gratissimum and Chromolaena odorata. To do this, three different doses (33 g/l; 39 g/l and 50 g/l) of aqueous extracts of Ocimum gratissimum and Chromolaena odorata were applied to yam slices before inoculation with Colletotrichum sp. Results showed that applying different doses of aqueous plant extract to yam slices before inoculation of the fungal strain caused less rot. The aqueous extracts of the two plants showed anti...
Today, one major practice to control diseases and pests in banana fields is the use of in vitro p... more Today, one major practice to control diseases and pests in banana fields is the use of in vitro plantlets. However, nematodes, in particular Radopholus similis, remain a major problem. The restriction on the use of nematicides has encouraged the development of alternative strategies, among which the use of biological control organisms (BCO) such as arbuscular mycorrhizal fungi (AMF). Their beneficial effects on the control of nematodes have been demonstrated but their application in the field remains rare. In the recent decade the association of BCO to plants at the in vitro stage, has been proposed to produce plants more resistant to pathogens and to transplant stresses. The in vitro association of AMF to banana plantlets may thus represent an adequate system to investigate the interaction with nematodes and for the mass-production of mycorrhized plantlets. Here we developed an in vitro culture system for the mycorrhization of banana plantlets. It was used to study the interactions...
RESUME Le Niébé (Vigna unguiculata) est une légumineuse alimentaire qui fournit plus de la moitié... more RESUME Le Niébé (Vigna unguiculata) est une légumineuse alimentaire qui fournit plus de la moitié des protéines consommés. Cependant, sa culture est sujette à l'attaque de nombreux pathogènes et les rendements restent faibles. Une des alternatives pour un meilleur rendement est l'utilisation des produits agrochimiques. L'objectif recherché dans cette étude est d'optimiser la production de Vigna unguiculata. Pour atteindre cet objectif, des expérimentations ont été entreprises pour évaluer l'effet de l'utilisation du Furadan, du Mancomax et du Forum sur la nodulation lors de la culture de deux accessions (NbnK et NnB) de niébé. Pour ce faire, le sol de culture provenant d'une jachère de 2 ans au sein de l'université a été traité avec le Furadan 3 jours avant le semis tandis qu'un traitement foliaire a été effectué avec le Mancomax, et Forum à la dose du fabricant après la levée des plants de 5 jours. Un second traitement a été fait trois semaines après le semis. Les résultats montrent que les trois fongicides testés réduisent significativement de 50 à 84% le nombre de nodules des deux variétés et les nodules de l'accession NnB sont plus réduits que celles de l'accession NbnK.
Brown stem rot (BSR) is one of the most important diseases of soybean (Glycine max (L.) Merr.) in... more Brown stem rot (BSR) is one of the most important diseases of soybean (Glycine max (L.) Merr.) in the north-central soybean-production region (10), yet the causal agent of the disease is poorly known. The disease is characterized by a browning of the vascular system and pith, and characteristic chlorosis and necrosis of the foliage develops late in the season, usually in August (2). However, similar vascular discoloration is found late in the season with other diseases of soybean. Some BSR-resistant germ plasm is available (3,24), mostly identified by the extent of vascular browning, but development of resistant cultivars and deployment of resistance have been hampered by imprecise resistance rating, a poor understanding of the causal agent of the disease, and uncertainty about the variation in aggressiveness in the pathogen (16,24,29,33). The pathogen was described as Cephalosporium gregatum in 1948 by Allington and Chamberlain (2). Unfortunately, the fungus forms rather nondescript conidiophores, phialides, and conidia, and many unrelated species are morphologically similar. Many Cephalosporium spp. are now placed in the heterogeneous genus Acremonium, but the proper generic placement of C. gregatum is still in question. Gams (14) transferred the species to the genus Phialophora, though the fungus generally produces non-pigmented conidiophores. Sequences of ribosomal DNA suggest that Phialophora gregata is an anamorph of a discomycete (T. C. Harrington, unpublished). Two or more Acremonium-type fungi that are similar to P. gregata have been associated with brown stem rot in soybean and adzuki bean (5,18,23), and at least one of these Acremonium spp. causes vascular discoloration in soybean (23). Gray (16) reported that the disease in soybean is caused by either of two forms of P. gregata, a defoliating form (type I) and a nondefoliating form (type II), with the latter causing vascular discoloration but no foliar symptoms. Gray and Hepburn (18) did not
In Vitro Cellular & Developmental Biology - Plant
Micropropagated banana plantlets represent a good planting material to establish new growing area... more Micropropagated banana plantlets represent a good planting material to establish new growing areas or replace old plantations. The plantlets are devoid of pathogens but also of beneficial root inhabitants (e.g., endophytes and arbuscular mycorrhizal fungi [AMF]) that may help plants withstand stresses. Mycorrhization of banana is usually conducted at the hardening/post-hardening phases. Only a few studies have reported their application in vitro, and none have investigated the subsequent impact on ex vitro acclimatization. Here, we report for the first time the effects of in vitro mycorrhization of banana plantlets on growth following transfer to soil. Banana plantlets were pre-mycorrhized under autotrophic conditions. During in vitro culture, heavy root colonization of the banana plantlets was observed. At 5 and 7 wk after transfer to a peat-sand soil substrate, the root system remained highly colonized. The growth (pseudostem height) and biomass were significantly higher than thos...
Études internationales, 1997
Tous droits réservés © Études internationales, 1997 Ce document est protégé par la loi sur le dro... more Tous droits réservés © Études internationales, 1997 Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y compris la reproduction) est assujettie à sa politique d'utilisation que vous pouvez consulter en ligne.