Mariko Matsuda Alexander - Academia.edu (original) (raw)
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Papers by Mariko Matsuda Alexander
Current Issues in Molecular Biology, 2020
Virus research, Sep 11, 2017
Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and ... more Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and insect vectors are governed by the topology of the viral capsid, which is the sole vehicle for long distance movement of the viral genome. Previous application of a mass spectrometry-compatible cross-linker to preparations of the luteovirid Potato leafroll virus (PLRV; Luteoviridae: Polerovirus) revealed a detailed network of interactions between viral structural proteins and enabled generation of the first cross-linking guided coat protein models. In this study, we extended application of chemical cross-linking technology to the related Turnip yellows virus (TuYV; Luteoviridae: Polerovirus). Remarkably, all cross-links found between sites in the viral coat protein found for TuYV were also found in PLRV. Guided by these data, we present two models for the TuYV coat protein trimer, the basic structural unit of luteovirid virions. Additional cross-links found between the TuYV coat protein ...
Molecular & cellular proteomics : MCP, Apr 8, 2016
The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteovirid... more The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip as compared to the weed physalis, a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared to turnip-reared aphids was observed at 24h and 72h after virus acquisition. The major difference in the proteomes of these aphids was the upregulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there is near complete co-localization of cathB and PLRV at the cell membranes, which wa...
Current Plant Biology, 2016
Plant pathogenic viruses cause a number of economically important diseases in food, fuel, and fib... more Plant pathogenic viruses cause a number of economically important diseases in food, fuel, and fiber crops worldwide. As obligate parasites with highly reduced genomes, viruses rely heavily on their hosts for replication, assembly, intra-and intercellular movement, and attraction of vectors for dispersal. Therefore, viruses must influence or directly utilize many host proteins and processes. While many general effects of virus infection have long been known (e.g., reduction in photosynthesis, alterations in carbon metabolism and partitioning, increased expression of pathogenesis-related proteins), the precise underlying mechanisms and functions in the viral life cycle are largely a mystery. Proteomic studies, including studies of differential protein regulation during infection as well as studies of host-viral protein-protein interactions, can help shed light on the complex and varied molecular interactions between viruses and plant hosts. In this review, we summarize current literature in plant-virus proteomics and speculate on why viruses have been selected to manipulate these diverse biochemical pathways in their plant hosts.
Fungal Genetics and Biology, 2014
Journal of virology, Feb 9, 2015
Demonstrating direct interactions between host and virus proteins during infection is a major goa... more Demonstrating direct interactions between host and virus proteins during infection is a major goal and challenge for the field of virology. Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (Potato leafroll virus, PLRV) and Protein Interaction Reporter (PIR), a revolutionary technology that couples a mass spectrometric-cleavable chemical cross-linker with high resolution mass spectrometry, we provide the first report of any host-pathogen protein interaction network that includes data-derived, topological features for every cross-linked site that was identified. We show that PLRV virions have hot spots of protein interaction and multifunctional surface topologies, revealing how these plant viruses maximize their use of protein interaction interfaces. Modeling data, guided by cross-linking constraints, suggest asymmetric packing of the major capsid protein in the virion in line with previous epitope ...
Current Issues in Molecular Biology, 2020
Virus research, Sep 11, 2017
Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and ... more Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and insect vectors are governed by the topology of the viral capsid, which is the sole vehicle for long distance movement of the viral genome. Previous application of a mass spectrometry-compatible cross-linker to preparations of the luteovirid Potato leafroll virus (PLRV; Luteoviridae: Polerovirus) revealed a detailed network of interactions between viral structural proteins and enabled generation of the first cross-linking guided coat protein models. In this study, we extended application of chemical cross-linking technology to the related Turnip yellows virus (TuYV; Luteoviridae: Polerovirus). Remarkably, all cross-links found between sites in the viral coat protein found for TuYV were also found in PLRV. Guided by these data, we present two models for the TuYV coat protein trimer, the basic structural unit of luteovirid virions. Additional cross-links found between the TuYV coat protein ...
Molecular & cellular proteomics : MCP, Apr 8, 2016
The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteovirid... more The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip as compared to the weed physalis, a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared to turnip-reared aphids was observed at 24h and 72h after virus acquisition. The major difference in the proteomes of these aphids was the upregulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there is near complete co-localization of cathB and PLRV at the cell membranes, which wa...
Current Plant Biology, 2016
Plant pathogenic viruses cause a number of economically important diseases in food, fuel, and fib... more Plant pathogenic viruses cause a number of economically important diseases in food, fuel, and fiber crops worldwide. As obligate parasites with highly reduced genomes, viruses rely heavily on their hosts for replication, assembly, intra-and intercellular movement, and attraction of vectors for dispersal. Therefore, viruses must influence or directly utilize many host proteins and processes. While many general effects of virus infection have long been known (e.g., reduction in photosynthesis, alterations in carbon metabolism and partitioning, increased expression of pathogenesis-related proteins), the precise underlying mechanisms and functions in the viral life cycle are largely a mystery. Proteomic studies, including studies of differential protein regulation during infection as well as studies of host-viral protein-protein interactions, can help shed light on the complex and varied molecular interactions between viruses and plant hosts. In this review, we summarize current literature in plant-virus proteomics and speculate on why viruses have been selected to manipulate these diverse biochemical pathways in their plant hosts.
Fungal Genetics and Biology, 2014
Journal of virology, Feb 9, 2015
Demonstrating direct interactions between host and virus proteins during infection is a major goa... more Demonstrating direct interactions between host and virus proteins during infection is a major goal and challenge for the field of virology. Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (Potato leafroll virus, PLRV) and Protein Interaction Reporter (PIR), a revolutionary technology that couples a mass spectrometric-cleavable chemical cross-linker with high resolution mass spectrometry, we provide the first report of any host-pathogen protein interaction network that includes data-derived, topological features for every cross-linked site that was identified. We show that PLRV virions have hot spots of protein interaction and multifunctional surface topologies, revealing how these plant viruses maximize their use of protein interaction interfaces. Modeling data, guided by cross-linking constraints, suggest asymmetric packing of the major capsid protein in the virion in line with previous epitope ...