Marilene Da Silva - Academia.edu (original) (raw)

Papers by Marilene Da Silva

Revista Feridas

Objetivo: Apresentar os resultados obtidos no tratamento com fotobiomodulação associado as terapi... more Objetivo: Apresentar os resultados obtidos no tratamento com fotobiomodulação associado as terapias tópicas de hidrogel e ácido hialurônico 0,2% (AH) em lesões por deiscência cirúrgica de mastoplastia de aumento. Metodologia: Trata-se de um estudo descritivo exploratório de relato de experiência de uma mulher, 26 anos, sem comorbidades, que desenvolveu deiscência de sutura após submeter-se a uma mastoplastia de aumento. Na abordagem inicial, preconizou-se o protocolo com aplicação semanal de fotobiomodulação (660nm) combinado com administração tópica diária de hidrogel. Na finalização do tratamento optou-se pelo uso tópico de AH 0,2% , visando a melhora da estética do processo cicatricial. A análise dos efeitos dos tratamentos foi realizada por meio de registro fotográfico com câmera digital 16 megapixels, a uma distância de 20 centímetros. As imagens foram examinadas por meio do software ImageJ. Resultados: O fechamento da lesão ocorreu após 90 dias do início do tratamento. A repar...

This file contains the supplementary data of the article published at Plos One "Topical use ... more This file contains the supplementary data of the article published at Plos One "Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats".All relevant information, such as methodology, results and discussion can be found with the corresponding author (Bruno G Lania) or at Plos One website and others website in which this journal in indexed.<br>10.1371/journal.pone.0188779

This study aims to report the effectiveness of low power laser (LPL) treatment associated with to... more This study aims to report the effectiveness of low power laser (LPL) treatment associated with topical 0.2% hyaluronic acid (HA) therapy in traumatic lesions in a female elderly patient. The present study reports the clinical case of a patient with self-induced traumatic injury in the trochanter region, treated with HA 0.2% coverage combined with LPL. This study is part of an experimental research project coordinated by teachers and nurses who work on the development of new protocols for wound treatment. The patient D.M.M.N. was 74 years old, hypertensive, diabetic, with grade I obesity, with diagnosis of chronic ulcerative lesion in the left lower limb trochanter region due to intense itching caused by skin dryness. Initially, a wound bed preparation protocol with the use of hydrogel and conservative instrumental debridement was recommended, followed by a second protocol with weekly LPL application, combined with daily topical administration of HA 0.2%. The analysis of the effects of the treatments was performed by means of photographic record and the images were analyzed with the aid of the software ImageJ. The dressings were performed daily and, in 49 days, the total healing of the lesion was observed. No discomfort or complications due to the use of the products and LPL were observed. It was concluded that the proposed therapy presented good tolerability and therapeutic ef cacy for this particular case. Descriptors: wounds and injuries; low-level light therapy; hyaluronic acid; wound healing; wound closure techniques.

Revista Feridas, 2022

Objetivos: Este estudo tem por objetivo relatar a efetividade do laser de baixa potência (LBP) as... more Objetivos: Este estudo tem por objetivo relatar a efetividade do laser de baixa potência (LBP) associado à terapia tópica com Ácido Hialurônico (AH) 0,2% em dermatite de contato alérgica. Metodologia: Trata-se de estudo descritivo exploratório de relato de experiência com paciente assistida em domicílio por uma enfermeira especialista em feridas em uma cidade do interior paulista. Preconizou-se protocolo com aplicação a cada 48 horas de LBP combinado com administraçãotópica diária de AH a 0,2%. A análise dos efeitos do tratamento foi realizada por meio de registro fotográfico e as imagens foram analisadas com auxílio do software ImageJ. Os curativos foram realizados diariamente e, em 17 dias, observou-se a total cicatrização da lesão. Resultados: Ao final de 17 dias a paciente apresentou cicatrização completa. cicatrização completa, e considerando o fototipo cutâneo da paciente, a mesma foi orientada a dar continuidade ao tratamento diário com aplicação tópica de AH 0,2% por mais 15...

Revista Feridas, 2021

Objetivo: Este estudo busca expor evidências obtidas numa proposta terapêutica de relatar a efeti... more Objetivo: Este estudo busca expor evidências obtidas numa proposta terapêutica de relatar a efetividade do tratamento com laser de baixa potência (LBP) associado a terapia tópica de PHMB em lesões crônicas. Metodologia: Trata-se de um relato de experiência com uma paciente assistida pelas Clínicas de Enfermagem e de Fisioterapia de uma faculdade do interior paulista. A paciente M.C.S., 68 anos, hipertensa, diabética, obesidade grau I, com diagnóstico de lesão ulcerativa crônica região infrapatelar de membro inferior direito devido uso de órtese. Preconizou-se um protocolo de uma aplicação semanal de LBP, antes da administração tópica de PHMB nas lesões. A análise dos efeitos dos tratamentos foi realizada através de registro fotográfico e as imagens foram projetadas para o software ImageJ. Resultados: A paciente obteve 100% de presença, em 7 sessões prescritas de LBP com PHMB e também fez uso de PHMB em sua lesão todos os dias no domicílio. A lesão fechou-se após 43 dias do início do...

Anais Brasileiros de Dermatologia, 2021

Vector-Borne and Zoonotic Diseases, 2020

Background: The inherent characteristics of the sickle cell disease (SCD), the most common geneti... more Background: The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections. Bartonella spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients. Materials and Methods: We evaluated Bartonella spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of Bartonella spp. DNA. Bartonella DNA was amplified using conventional genus-specific Bartonella PCR which amplifies the Intergenic Transcribed Spacer region and Bartonella henselae-specific nested PCR which amplifies the FtsZ gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced. Findings: Ten of 107 patients tested positive for B. henselae infection in at least one molecular test. All obtained amplicons were sequenced and similar to B. henselae sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with B. henselae was not associated with animal contact or blood transfusions. Conclusion: We detected B. henselae DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them.

PloS one, 2017

Wounds are a common health problem. Coffee is widely consumed and its oil contains essential fatt... more Wounds are a common health problem. Coffee is widely consumed and its oil contains essential fatty acids. We evaluated the local (skin) and systemic effects associated with the topical use of coffee oils in rats. Punch skin wounds (6 mm) incisions were generated on the backs of 75 rats. Saline (SS), mineral oil (MO), green coffee oil (GCO), roasted coffee oil (RCO), green coffee ground oil (GCGO) or roasted coffee ground oil (RCGO) were topically applied to the wounds. Healing was evaluated by visual and histological/morphometric optical microscopy examination; second harmonics generation (SHG) microscopy, wound tissue q-PCR (values in fold-change) and blood serum (ELISA, values in pg/mL). RCO treated animals presented faster wound healing (0.986 vs. 0.422), higher mRNA expression of IGF-1 (2.78 vs. 1.00, p = 0.01), IL-6 (10.72 vs. 1.00, p = 0.001) and IL-23 (4.10 vs. 1.2, p = 0.05) in early stages of wound healing; higher IL-12 (3.32 vs. 1.00, p = 0.05) in the later stages; and low...

Microscopy and Microanalysis, 2015

Bartonella spp. can induce long-lasting bacteremia in mammals (1). Bartonella spp. are causative ... more Bartonella spp. can induce long-lasting bacteremia in mammals (1). Bartonella spp. are causative agents of cat-scratch disease, endocarditis, bacillary angiomatosis, bacillary peliosis, trench fever, neurocognitive dysfunction and regional pain syndrome (2). Chronic Bartonella spp. infection and subsequent manifestations of persistent infection may be the result of the bacteria growing in biofilms. In humans, at least four Bartonella species have been associated with infectious endocarditis, a biofilmassociated infectious disease (3). Biofilms are sessile communities of interacting unicellular organisms that adhere to a surface and to each other and create unique, complex structures, covered with a protective layer of extracellular polymeric substances (EPS). Biofilms and their inherent resistance to antimicrobial agents are often key players in many persistent and chronic bacterial infections (4, 5). We acquired skin biopsies from 5 patients with blood PCR-positive B. henselae. 60-100 micron-thick sections were immunostained with antibodies to B. henselae. Stained sections were imaged using both single-and multi-photon imaging to capture epifluorescence emission and second harmonic generation signal of non-stained collagen I and II (Fig 1). The crystalline triple-helix of native fibrillar collagen (I, II, III and V) generates a strong second harmonic generation (SHG) signal (6). In patients with longstanding bartonellosis, clusters of immuno-reactive B. henselae were found intimately associated with fibrillar dermal collagen (Fig 2). Additionally, we found a Bartonella spp. biofilm on a peripheral intravenous catheter line removed from a patient with persistent Bartonella (Fig 3). Further, B. henselae, cultured 14 or 21 days, on collagen I and II-coated coverslips revealed a B. henselae biofilm matrix (Fig 4). Identification and characterization of Bartonella spp. biofilms in vivo and in situ will lead to a better understanding of persistent Bartonellosis and provide direction in treatment modalities.

Biodiversidade e Biotecnologia no Brasil, 2020

RESUMO O presente estudo documenta a diversidade morfológica de escamas de 18 espécies representa... more RESUMO O presente estudo documenta a diversidade morfológica de escamas de 18 espécies representando 12 famílias de Characiformes Neotropicais. O material foi coletado de exemplares comercializados do Mercado de Peixes Resene de Souza Lima, em Cruzeiro do Sul, Acre, Brasil, com procedência da bacia do Alto Rio Juruá. As escamas foram extraídas de 15 regiões do flanco esquerdo de cada exemplar, limpas mecanicamente e coradas com Alizarina para posterior investigação macroscópica, documentação fotográfica e descrições. Os resultados obtidos apontam que existe grande variação na forma das escamas das famílias examinadas. Estruturas como dentículos, canais interfaciais ou poros, escamas com parte posterior membranosa, escamas espinóides e crenadas são, pela primeira vez, descritas em Characiformes do Vale do Alto Rio Juruá. Diante da importância da morfologia das escamas ctenóides, crenadas e espinóides para fins de classificação dos Actinopterígios, essas variações morfológicas observadas nas famílias de Characiformes podem representar um sinal filogenético, que deverão ser analisados em um contexto sistemático mais abrangente com o estudo da lepidologia em outros representantes da ordem. Palavras-chave: Lepidologia, Peixes Neotropicais e Descrição Morfológica.

Hybrid teaching is certainly an exponential as an educational trend in contemporary times, aiming... more Hybrid teaching is certainly an exponential as an educational trend in contemporary times, aiming to bring new challenges and solutions in teaching, in the EAD modality. This combination of online and face-to-face learning, integrating into the student's day-to-day life, leads to a favorable and familiar environment to the study program. In this perspective, we have virtual environments (AVA) that are technological information devices (ICT), which aggregate various digital resources that have the intention and interest focused on learning, with the possibilities of game programs. These arose from the phenomenon of gamification or games applied to education, in a constructivist perspective as an educational strategy, allowing development and playful access within the educational framework, and motivating students. Developed from the theory of educational games, it should be considered that the rewards are related to productive satisfaction, not as a competitive award among studen...