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Papers by Marina Damaceno

Research paper thumbnail of Sepsis expands a CD39+ plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity

Immunity, 2021

Sepsis results in elevated adenosine in circulation. Extracellular adenosine triggers immunosuppr... more Sepsis results in elevated adenosine in circulation. Extracellular adenosine triggers immunosuppressive signaling via the A2a receptor (A2aR). Sepsis survivors develop persistent immunosuppression with increased risk of recurrent infections. We utilized the cecal ligation and puncture (CLP) model of sepsis and subsequent infection to assess the role of adenosine in post-sepsis immune suppression. A2aR-deficient mice showed improved resistance to post-sepsis infections. Sepsis expanded a subset of CD39hi B cells and elevated extracellular adenosine, which was absent in mice lacking CD39-expressing B cells. Sepsis-surviving B cell-deficient mice were more resistant to secondary infections. Mechanistically, metabolic reprogramming of septic B cells increased production of ATP, which was converted into adenosine by CD39 on plasmablasts. Adenosine signaling via A2aR impaired macrophage bactericidal activity and enhanced interleukin-10 production. Septic individuals exhibited expanded CD39hi plasmablasts and adenosine accumulation. Our study reveals CD39hi plasmablasts and adenosine as important drivers of sepsis-induced immunosuppression with relevance in human disease.

Research paper thumbnail of Desenvolvimento de método analítico empregando QuEChERS para a determinação simultânea de resíduos multiclasses de fármacos veterinários em pescados

Damaceno, A. M. Development of an analytical method for simultaneous determination of multiclass ... more Damaceno, A. M. Development of an analytical method for simultaneous determination of multiclass veterinary drugs in fish using LC-MS. 2017. 98p.

Research paper thumbnail of Diagnóstico da Esclerose Múltipla por Imagem

Id on Line REVISTA DE PSICOLOGIA, 2018

A Esclerose Múltipla (EM) é conhecida como uma doença autoimune, desmielinizante e inflamatória d... more A Esclerose Múltipla (EM) é conhecida como uma doença autoimune, desmielinizante e inflamatória da qual não possui cura podendo atingir pessoas entre 20 - 40 anos de idade sendo mais frequente em mulheres e pessoas da raça branca. Ela se apresenta de quatro formas clínicas podendo ser recorrente remitente, primária progressiva, secundária progressiva e progressiva recorrente das quais serão abordadas com mais detalhes na introdução deste. O presente trabalho irá abordar através de revisão literária os sintomas da EM como perda da atividade motora, fadiga, tonturas, entre outras; irá apresentar o diagnóstico por imagem, enfatizando como o diagnóstico da EM através da Ressonância Magnética (RM) é crucial para a descoberta precoce da doença, quais os critérios exigidos para a realização do exame e o porquê deste exame ser considerado um padrão- ouro para diagnóstico da EM, mostrará ainda quais são os tratamentos disponíveis e quais são os mais indicados e utilizados para o tratamento d...

Research paper thumbnail of Food safety evaluation for the use of albendazole in fish: residual depletion profile and withdrawal period estimation

Food Additives & Contaminants: Part A, 2020

ABSTRACT Due to the lack of drugs regulated for aquaculture, we have evaluated the use of albenda... more ABSTRACT Due to the lack of drugs regulated for aquaculture, we have evaluated the use of albendazole (ABZ) – a potential drug to be regulated for fish – under food safety perspectives assessing the depletion profile of ABZ and its main metabolites (albendazole sulphoxide – ABZSO, albendazole sulphone – ABZSO2 and albendazole amino sulphone – ABZ-2-NH2SO2) in fish fillets (muscle and skin) after single dose oral administration of 10 mg ABZ kg-1 body weight. For the drug administration, a suitable procedure for ABZ incorporation into fish feed was employed, obtaining good homogeneity of ABZ concentration among feed pellets (CV<4.1%) and low drug leaching when medicated feed remained in the water for up to 60 min (<2.7%). After medication, fish were euthanised at 8, 12, 24, 48, 72, 96 and 120 h and fillets collected. Depletion studies in various fish species (patinga and tilapia) were conducted simultaneously, under water temperature at 30.4 ± 0.3 °C and pH 6.8 ± 0.1. The highest concentrations for the sum of residues (ABZ, ABZSO, ABZSO2 and ABZ-2-NH2SO2) in fish fillet were 1210 ng g-1 in patinga and 637 ng g−1 in tilapia. Under the employed rearing conditions, the obtained results did not indicate a requirement for a minimum withdrawal period to be proposed for tilapia considering the maximum residue limit of 100 µg g−1, since the determined residual concentration was <LOQ (10 ng g−1) from 24 h post medication treatment. For patinga, the estimated minimum withdrawal period was 3 days. With the well-recognised demands for new alternative veterinary drugs for aquaculture use by fish farmers, this study offers important evidence for consideration of ABZ use in tilapia and patinga taking into account food safety issues. Graphical abstract

Research paper thumbnail of Sepsis expands a CD39+ plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity

Research paper thumbnail of Sepsis expands a CD39+ plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity

Immunity, 2021

Sepsis results in elevated adenosine in circulation. Extracellular adenosine triggers immunosuppr... more Sepsis results in elevated adenosine in circulation. Extracellular adenosine triggers immunosuppressive signaling via the A2a receptor (A2aR). Sepsis survivors develop persistent immunosuppression with increased risk of recurrent infections. We utilized the cecal ligation and puncture (CLP) model of sepsis and subsequent infection to assess the role of adenosine in post-sepsis immune suppression. A2aR-deficient mice showed improved resistance to post-sepsis infections. Sepsis expanded a subset of CD39hi B cells and elevated extracellular adenosine, which was absent in mice lacking CD39-expressing B cells. Sepsis-surviving B cell-deficient mice were more resistant to secondary infections. Mechanistically, metabolic reprogramming of septic B cells increased production of ATP, which was converted into adenosine by CD39 on plasmablasts. Adenosine signaling via A2aR impaired macrophage bactericidal activity and enhanced interleukin-10 production. Septic individuals exhibited expanded CD39hi plasmablasts and adenosine accumulation. Our study reveals CD39hi plasmablasts and adenosine as important drivers of sepsis-induced immunosuppression with relevance in human disease.

Research paper thumbnail of Desenvolvimento de método analítico empregando QuEChERS para a determinação simultânea de resíduos multiclasses de fármacos veterinários em pescados

Damaceno, A. M. Development of an analytical method for simultaneous determination of multiclass ... more Damaceno, A. M. Development of an analytical method for simultaneous determination of multiclass veterinary drugs in fish using LC-MS. 2017. 98p.

Research paper thumbnail of Diagnóstico da Esclerose Múltipla por Imagem

Id on Line REVISTA DE PSICOLOGIA, 2018

A Esclerose Múltipla (EM) é conhecida como uma doença autoimune, desmielinizante e inflamatória d... more A Esclerose Múltipla (EM) é conhecida como uma doença autoimune, desmielinizante e inflamatória da qual não possui cura podendo atingir pessoas entre 20 - 40 anos de idade sendo mais frequente em mulheres e pessoas da raça branca. Ela se apresenta de quatro formas clínicas podendo ser recorrente remitente, primária progressiva, secundária progressiva e progressiva recorrente das quais serão abordadas com mais detalhes na introdução deste. O presente trabalho irá abordar através de revisão literária os sintomas da EM como perda da atividade motora, fadiga, tonturas, entre outras; irá apresentar o diagnóstico por imagem, enfatizando como o diagnóstico da EM através da Ressonância Magnética (RM) é crucial para a descoberta precoce da doença, quais os critérios exigidos para a realização do exame e o porquê deste exame ser considerado um padrão- ouro para diagnóstico da EM, mostrará ainda quais são os tratamentos disponíveis e quais são os mais indicados e utilizados para o tratamento d...

Research paper thumbnail of Food safety evaluation for the use of albendazole in fish: residual depletion profile and withdrawal period estimation

Food Additives & Contaminants: Part A, 2020

ABSTRACT Due to the lack of drugs regulated for aquaculture, we have evaluated the use of albenda... more ABSTRACT Due to the lack of drugs regulated for aquaculture, we have evaluated the use of albendazole (ABZ) – a potential drug to be regulated for fish – under food safety perspectives assessing the depletion profile of ABZ and its main metabolites (albendazole sulphoxide – ABZSO, albendazole sulphone – ABZSO2 and albendazole amino sulphone – ABZ-2-NH2SO2) in fish fillets (muscle and skin) after single dose oral administration of 10 mg ABZ kg-1 body weight. For the drug administration, a suitable procedure for ABZ incorporation into fish feed was employed, obtaining good homogeneity of ABZ concentration among feed pellets (CV<4.1%) and low drug leaching when medicated feed remained in the water for up to 60 min (<2.7%). After medication, fish were euthanised at 8, 12, 24, 48, 72, 96 and 120 h and fillets collected. Depletion studies in various fish species (patinga and tilapia) were conducted simultaneously, under water temperature at 30.4 ± 0.3 °C and pH 6.8 ± 0.1. The highest concentrations for the sum of residues (ABZ, ABZSO, ABZSO2 and ABZ-2-NH2SO2) in fish fillet were 1210 ng g-1 in patinga and 637 ng g−1 in tilapia. Under the employed rearing conditions, the obtained results did not indicate a requirement for a minimum withdrawal period to be proposed for tilapia considering the maximum residue limit of 100 µg g−1, since the determined residual concentration was <LOQ (10 ng g−1) from 24 h post medication treatment. For patinga, the estimated minimum withdrawal period was 3 days. With the well-recognised demands for new alternative veterinary drugs for aquaculture use by fish farmers, this study offers important evidence for consideration of ABZ use in tilapia and patinga taking into account food safety issues. Graphical abstract

Research paper thumbnail of Sepsis expands a CD39+ plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity