Marine Hugonnard - Academia.edu (original) (raw)

Papers by Marine Hugonnard

Research paper thumbnail of Hemorrhagic, Hemostatic, and Thromboelastometric Disorders in 35 Dogs with a Clinical Diagnosis of Leptospirosis: A Prospective Study

Journal of Veterinary Internal Medicine, Dec 2, 2016

Background: Leptospirosis in dogs is occasionally associated with a hemorrhagic syndrome, the pat... more Background: Leptospirosis in dogs is occasionally associated with a hemorrhagic syndrome, the pathophysiology of which is not fully understood. Hypothesis/Objectives: To characterize hematologic, hemostatic, and thromboelastometric abnormalities in dogs with leptospirosis and to study their association with hemorrhagic diatheses and outcomes. Animals: Thirty-five client-owned dogs. Methods: A prospective observational single cohort study was conducted. Results from the CBC, coagulation tests (prothrombin, activated partial thromboplastin and thrombin times, fibrinogen, fibrin(ogen) degradation products, and D-dimer concentrations), rotational thromboelastometry (TEM), signalment, hemorrhagic diatheses, occurrence of disseminated intravascular coagulation (DIC) at admission, and survival to discharge were recorded. Results: The most common hematologic and hemostatic abnormalities were anemia (30/35), thrombocytopenia (21/35), and hyperfibrinogenemia (15/35). Eight dogs were diagnosed with DIC. A normal TEM profile was found in 14 dogs, a hypercoagulable profile in 14 dogs, and a hypocoagulable profile in 7 dogs. The 8 dogs with hemorrhagic diatheses at admission had significantly decreased platelet counts (P = .037) and increased D-dimer concentrations (P = .015) compared with other dogs. Dogs with a hypocoagulable profile exhibited more hemorrhagic diatheses compared with the dogs that had normal and hypercoagulable profiles (P = .049). The mortality rate was lower in dogs with a hypercoagulable profile than in those with a hypocoagulable profile (21% vs 57%; P = .043). Disseminated intravascular coagulation was not a significant prognostic factor. Conclusions and Clinical Importance: Thromboelastometric parameters were altered in dogs with both hypercoagulable and hypocoagulable profiles. A hypocoagulable profile was significantly correlated with hemorrhagic diathesis and higher mortality rate.

Research paper thumbnail of Recurrence and survival in dogs with excised colorectal polyps: A retrospective study of 58 cases

Journal of Veterinary Internal Medicine

BackgroundCompared to humans, colorectal polyps are relatively rare in dogs. Epidemiological and ... more BackgroundCompared to humans, colorectal polyps are relatively rare in dogs. Epidemiological and prognostic data remain accordingly sparse, although they could help veterinary clinicians in the management of these cases.ObjectivesTo report the epidemiological data of dogs with colorectal polyps and identify factors associated with recurrence and survival.AnimalsFifty‐eight client‐owned dogs with colorectal polyps admitted to 7 veterinary hospitals (53 dogs from France, 5 dogs from Spain, and 4 dogs from Portugal) were included.MethodsRetrospective multicentric cohort study. Medical records and long‐term outcome of the dogs were reviewed. When available, histological samples were reassessed by 2 board‐certified pathologists according to the revised Vienna classification (RVC).ResultsThe West Highland White Terrier (WHWT) breed was significantly associated with the presence of colorectal polyps (OR: 20; 95% CI: 7.5‐52; P < .001). The overall median time to recurrence was not reache...

Research paper thumbnail of Canine intestinal lymphangiectasia: retrospective study of 19 dogs

Le Nouveau Praticien Vétérinaire Canine - Féline, 2016

Research paper thumbnail of Comparison between serology and PCR in the diagnosis of canine leptospirosis

The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs... more The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs are available for practitioners to diagnose canine leptospirosis. The microscopic agglutination test is still the reference test despite some known pitfalls (delayed serologic response with frequent negative titers in the first week of illness, vaccinal titers confusing interpretation of serologic results, possibility of titers resulting from previous exposure to pathogenic strains). The polymerase chain reaction could be helpful in the diagnosis, especially in the first week of illness or with recent vaccinal history. However some dogs can be asymptomatic carriers and shed leptospires in their urines. Moreover, there is currently limited information regarding the validity of polymerase chain reaction assays for detection of leptospirosis in dogs. As positive titers can be encountered in apparently healthy dogs and as asymptomatic shedding does exist in dogs, laboratory results must always be cautiously interpreted in conjunction with clinical picture. Currently, the microscopic agglutination test and the polymerase chain reaction appear to be complementary. They should be done concurrently and interpreted together in a specific medical context.

Research paper thumbnail of Vaccination contre la leptospirose canine en France : enquête sur les pratiques vétérinaires et leurs motivations

Revue Vétérinaire Clinique, Mar 1, 2023

Research paper thumbnail of Screening for Leptospira DNA in blood and urine from 30 apparently healthy dogs

Revue Vétérinaire Clinique, Sep 1, 2018

Asymptomatic carriage of leptospires in dogs has not been extensively studied. A prospective stud... more Asymptomatic carriage of leptospires in dogs has not been extensively studied. A prospective study was conducted on thirty apparently healthy male dogs to screen for Leptospiral DNA in their blood and urine. All dogs were up to date with a bivalent vaccine against Leptospirosis. Microscopic agglutination test (MAT) and real-time PCR analysis in blood and urine were performed for each dog. Six of 30 (20%) dogs had positive PCR results: two from blood samples (all MAT titres ≤ 160) and four from urine samples (one with maximal MAT titres of 2560 and three with all MAT titres ≤ 160). Ten months later, PCR analyses of blood and urine were repeated for these six dogs. Four had negative PCR results from blood and urine. The other two, with positive urine results at the first sampling, had negative PCR results from urine and positive PCR results from blood. In this study, the urinary shedding of leptospires appeared to be transient. As shown previously, serological status was not correlated with PCR results. Low but detectable amounts of Leptospiral DNA in the blood of some apparently healthy dogs raises questions regarding the pathophysiology of Leptospirosis and the diagnostic consequences of high-sensitivity PCR assays. Résumé Le portage asymptomatique des leptospires chez le chien est encore mal connu. Une étude prospective sur 30 chiens mâles apparemment sains a recherché la présence

Research paper thumbnail of Prevalence of Tritrichomonas foetus infections in French catteries

Veterinary Parasitology, Sep 1, 2013

Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the ass... more Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the assessment of Tritrichomonas foetus infection by polymerase chain reaction (PCR). Sixteen (14.4%) samples were found to be positive. In agreement with studies from the USA, infected cats were predominantly of a year of age or less and of a pedigree breed, with Siamese and Bengal cats specifically over-represented in this population.

Research paper thumbnail of Iatrogenic symptomatic hypoadrenocorticism after treatment with trilostane for hyperadrenocorticism in dogs: eight cases (2008‐2019)

Journal of Small Animal Practice

Research paper thumbnail of Small intestinal chronic diarrhoea in dogs and cats

Le Nouveau Praticien Vétérinaire Canine - Féline, 2016

Research paper thumbnail of Comparison between serology and PCR in the diagnosis of canine leptospirosis

The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs... more The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs are available for practitioners to diagnose canine leptospirosis. The microscopic agglutination test is still the reference test despite some known pitfalls (delayed serologic response with frequent negative titers in the first week of illness, vaccinal titers confusing interpretation of serologic results, possibility of titers resulting from previous exposure to pathogenic strains). The polymerase chain reaction could be helpful in the diagnosis, especially in the first week of illness or with recent vaccinal history. However some dogs can be asymptomatic carriers and shed leptospires in their urines. Moreover, there is currently limited information regarding the validity of polymerase chain reaction assays for detection of leptospirosis in dogs. As positive titers can be encountered in apparently healthy dogs and as asymptomatic shedding does exist in dogs, laboratory results must alw...

Research paper thumbnail of Differential diagnosis, diagnosis and treatment of nasal sinus cavity neoplasm in dogs

Research paper thumbnail of Novel dietary strategies can improve the outcome of weight loss programmes in obese client-owned cats

Journal of Feline Medicine and Surgery, 2010

A randomised, single-blinded, positively controlled, field trial for weight loss in obese client-... more A randomised, single-blinded, positively controlled, field trial for weight loss in obese client-owned cats was undertaken to look at novel diets and dietary strategies that could improve owner compliance and, therefore, success of feline weight loss programmes. Three dietary strategies were evaluated: strategy A used a novel dry high fibre ration; strategy B used ready-prepared portions of dry and moist food; strategy C used an existing commercial dry high fibre ration fed with a measuring cup. Cats were assessed at weeks 4, 12 and 20, and adjustments to the energy allocation made if required. Mean weight loss at 20 weeks (A: 11.0±1.3%, B: 10.9±1.2%, C: 11.9±1.7%) and mean energy allocation (A: 31.0, B: 28.5 and C: 32.2 kcal/kg of target body weight/day) were similar amongst strategies. However, owners' subjective hunger score was significantly ( P<0.001) higher with strategy C than with strategies A and B. Further, owner satisfaction was lowest with strategy C, and more own...

Research paper thumbnail of Assessment of inter-observer agreement among experienced and inexperienced observers and an automated device for dipstick urinalysis in dogs and cats

Journal of Veterinary Diagnostic Investigation

Standard visual urine dipstick analysis (UDA) is performed routinely in veterinary medicine; resu... more Standard visual urine dipstick analysis (UDA) is performed routinely in veterinary medicine; results can be influenced by both the operator and the method. We evaluated the agreement of results for canine and feline urine samples analyzed using a 10-patch dipstick (Multistix10SG; Siemens), both visually under double-anonymized conditions by students and a laboratory technician, and with an automated device (AD; Clinitek Status, Siemens). The mean concordance for semiquantitative urinalysis results between students and the technician and between students and the AD was fair (κ0.21–0.40) in dogs and cats; concordance was moderate between the technician and the AD (κ0.41–0.60) in dogs and good (κ0.61–0.80) in cats. For pH, the mean concordance between students and the technician and between the technician and the AD was good (ρ0.80–0.92) in dogs and cats; concordance was good between students and the AD (ρ0.80–0.92) in dogs and moderate (ρ0.59–0.79) in cats. Repeatability was higher ( ...

Research paper thumbnail of Spécificités des infections du tractus urinaire chez le chat

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Techniques non instrumentales de désencombrement bronchique chez le chien

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Iconographies supplémentaires de l'article: La leptospirose canine en France: étude rétrospective de 37 cas

HAL (Le Centre pour la Communication Scientifique Directe), 2010

Research paper thumbnail of Comment vos clients utilisent Internet

LYON1-BU Santé (693882101) / SudocSudocFranceF

Research paper thumbnail of Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptospirosis

Revue Vétérinaire Clinique, 2020

Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptos... more Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptospirosis L'atteinte pulmonaire en tant que composante d'un syndrome de défaillance multiviscérale chez les chiens atteints de leptospirose

Research paper thumbnail of Intérêts et limites de la coproculture dans le diagnostic des diarrhées d'origine bactérienne du chien et du chat

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Importance et place de la nutrition dans la prise en charge (étude bibliographique et étude clinique retrospective)

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Hemorrhagic, Hemostatic, and Thromboelastometric Disorders in 35 Dogs with a Clinical Diagnosis of Leptospirosis: A Prospective Study

Journal of Veterinary Internal Medicine, Dec 2, 2016

Background: Leptospirosis in dogs is occasionally associated with a hemorrhagic syndrome, the pat... more Background: Leptospirosis in dogs is occasionally associated with a hemorrhagic syndrome, the pathophysiology of which is not fully understood. Hypothesis/Objectives: To characterize hematologic, hemostatic, and thromboelastometric abnormalities in dogs with leptospirosis and to study their association with hemorrhagic diatheses and outcomes. Animals: Thirty-five client-owned dogs. Methods: A prospective observational single cohort study was conducted. Results from the CBC, coagulation tests (prothrombin, activated partial thromboplastin and thrombin times, fibrinogen, fibrin(ogen) degradation products, and D-dimer concentrations), rotational thromboelastometry (TEM), signalment, hemorrhagic diatheses, occurrence of disseminated intravascular coagulation (DIC) at admission, and survival to discharge were recorded. Results: The most common hematologic and hemostatic abnormalities were anemia (30/35), thrombocytopenia (21/35), and hyperfibrinogenemia (15/35). Eight dogs were diagnosed with DIC. A normal TEM profile was found in 14 dogs, a hypercoagulable profile in 14 dogs, and a hypocoagulable profile in 7 dogs. The 8 dogs with hemorrhagic diatheses at admission had significantly decreased platelet counts (P = .037) and increased D-dimer concentrations (P = .015) compared with other dogs. Dogs with a hypocoagulable profile exhibited more hemorrhagic diatheses compared with the dogs that had normal and hypercoagulable profiles (P = .049). The mortality rate was lower in dogs with a hypercoagulable profile than in those with a hypocoagulable profile (21% vs 57%; P = .043). Disseminated intravascular coagulation was not a significant prognostic factor. Conclusions and Clinical Importance: Thromboelastometric parameters were altered in dogs with both hypercoagulable and hypocoagulable profiles. A hypocoagulable profile was significantly correlated with hemorrhagic diathesis and higher mortality rate.

Research paper thumbnail of Recurrence and survival in dogs with excised colorectal polyps: A retrospective study of 58 cases

Journal of Veterinary Internal Medicine

BackgroundCompared to humans, colorectal polyps are relatively rare in dogs. Epidemiological and ... more BackgroundCompared to humans, colorectal polyps are relatively rare in dogs. Epidemiological and prognostic data remain accordingly sparse, although they could help veterinary clinicians in the management of these cases.ObjectivesTo report the epidemiological data of dogs with colorectal polyps and identify factors associated with recurrence and survival.AnimalsFifty‐eight client‐owned dogs with colorectal polyps admitted to 7 veterinary hospitals (53 dogs from France, 5 dogs from Spain, and 4 dogs from Portugal) were included.MethodsRetrospective multicentric cohort study. Medical records and long‐term outcome of the dogs were reviewed. When available, histological samples were reassessed by 2 board‐certified pathologists according to the revised Vienna classification (RVC).ResultsThe West Highland White Terrier (WHWT) breed was significantly associated with the presence of colorectal polyps (OR: 20; 95% CI: 7.5‐52; P < .001). The overall median time to recurrence was not reache...

Research paper thumbnail of Canine intestinal lymphangiectasia: retrospective study of 19 dogs

Le Nouveau Praticien Vétérinaire Canine - Féline, 2016

Research paper thumbnail of Comparison between serology and PCR in the diagnosis of canine leptospirosis

The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs... more The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs are available for practitioners to diagnose canine leptospirosis. The microscopic agglutination test is still the reference test despite some known pitfalls (delayed serologic response with frequent negative titers in the first week of illness, vaccinal titers confusing interpretation of serologic results, possibility of titers resulting from previous exposure to pathogenic strains). The polymerase chain reaction could be helpful in the diagnosis, especially in the first week of illness or with recent vaccinal history. However some dogs can be asymptomatic carriers and shed leptospires in their urines. Moreover, there is currently limited information regarding the validity of polymerase chain reaction assays for detection of leptospirosis in dogs. As positive titers can be encountered in apparently healthy dogs and as asymptomatic shedding does exist in dogs, laboratory results must always be cautiously interpreted in conjunction with clinical picture. Currently, the microscopic agglutination test and the polymerase chain reaction appear to be complementary. They should be done concurrently and interpreted together in a specific medical context.

Research paper thumbnail of Vaccination contre la leptospirose canine en France : enquête sur les pratiques vétérinaires et leurs motivations

Revue Vétérinaire Clinique, Mar 1, 2023

Research paper thumbnail of Screening for Leptospira DNA in blood and urine from 30 apparently healthy dogs

Revue Vétérinaire Clinique, Sep 1, 2018

Asymptomatic carriage of leptospires in dogs has not been extensively studied. A prospective stud... more Asymptomatic carriage of leptospires in dogs has not been extensively studied. A prospective study was conducted on thirty apparently healthy male dogs to screen for Leptospiral DNA in their blood and urine. All dogs were up to date with a bivalent vaccine against Leptospirosis. Microscopic agglutination test (MAT) and real-time PCR analysis in blood and urine were performed for each dog. Six of 30 (20%) dogs had positive PCR results: two from blood samples (all MAT titres ≤ 160) and four from urine samples (one with maximal MAT titres of 2560 and three with all MAT titres ≤ 160). Ten months later, PCR analyses of blood and urine were repeated for these six dogs. Four had negative PCR results from blood and urine. The other two, with positive urine results at the first sampling, had negative PCR results from urine and positive PCR results from blood. In this study, the urinary shedding of leptospires appeared to be transient. As shown previously, serological status was not correlated with PCR results. Low but detectable amounts of Leptospiral DNA in the blood of some apparently healthy dogs raises questions regarding the pathophysiology of Leptospirosis and the diagnostic consequences of high-sensitivity PCR assays. Résumé Le portage asymptomatique des leptospires chez le chien est encore mal connu. Une étude prospective sur 30 chiens mâles apparemment sains a recherché la présence

Research paper thumbnail of Prevalence of Tritrichomonas foetus infections in French catteries

Veterinary Parasitology, Sep 1, 2013

Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the ass... more Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the assessment of Tritrichomonas foetus infection by polymerase chain reaction (PCR). Sixteen (14.4%) samples were found to be positive. In agreement with studies from the USA, infected cats were predominantly of a year of age or less and of a pedigree breed, with Siamese and Bengal cats specifically over-represented in this population.

Research paper thumbnail of Iatrogenic symptomatic hypoadrenocorticism after treatment with trilostane for hyperadrenocorticism in dogs: eight cases (2008‐2019)

Journal of Small Animal Practice

Research paper thumbnail of Small intestinal chronic diarrhoea in dogs and cats

Le Nouveau Praticien Vétérinaire Canine - Féline, 2016

Research paper thumbnail of Comparison between serology and PCR in the diagnosis of canine leptospirosis

The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs... more The microscopic agglutination test as well as polymerase chain reaction on blood, urine or organs are available for practitioners to diagnose canine leptospirosis. The microscopic agglutination test is still the reference test despite some known pitfalls (delayed serologic response with frequent negative titers in the first week of illness, vaccinal titers confusing interpretation of serologic results, possibility of titers resulting from previous exposure to pathogenic strains). The polymerase chain reaction could be helpful in the diagnosis, especially in the first week of illness or with recent vaccinal history. However some dogs can be asymptomatic carriers and shed leptospires in their urines. Moreover, there is currently limited information regarding the validity of polymerase chain reaction assays for detection of leptospirosis in dogs. As positive titers can be encountered in apparently healthy dogs and as asymptomatic shedding does exist in dogs, laboratory results must alw...

Research paper thumbnail of Differential diagnosis, diagnosis and treatment of nasal sinus cavity neoplasm in dogs

Research paper thumbnail of Novel dietary strategies can improve the outcome of weight loss programmes in obese client-owned cats

Journal of Feline Medicine and Surgery, 2010

A randomised, single-blinded, positively controlled, field trial for weight loss in obese client-... more A randomised, single-blinded, positively controlled, field trial for weight loss in obese client-owned cats was undertaken to look at novel diets and dietary strategies that could improve owner compliance and, therefore, success of feline weight loss programmes. Three dietary strategies were evaluated: strategy A used a novel dry high fibre ration; strategy B used ready-prepared portions of dry and moist food; strategy C used an existing commercial dry high fibre ration fed with a measuring cup. Cats were assessed at weeks 4, 12 and 20, and adjustments to the energy allocation made if required. Mean weight loss at 20 weeks (A: 11.0±1.3%, B: 10.9±1.2%, C: 11.9±1.7%) and mean energy allocation (A: 31.0, B: 28.5 and C: 32.2 kcal/kg of target body weight/day) were similar amongst strategies. However, owners' subjective hunger score was significantly ( P<0.001) higher with strategy C than with strategies A and B. Further, owner satisfaction was lowest with strategy C, and more own...

Research paper thumbnail of Assessment of inter-observer agreement among experienced and inexperienced observers and an automated device for dipstick urinalysis in dogs and cats

Journal of Veterinary Diagnostic Investigation

Standard visual urine dipstick analysis (UDA) is performed routinely in veterinary medicine; resu... more Standard visual urine dipstick analysis (UDA) is performed routinely in veterinary medicine; results can be influenced by both the operator and the method. We evaluated the agreement of results for canine and feline urine samples analyzed using a 10-patch dipstick (Multistix10SG; Siemens), both visually under double-anonymized conditions by students and a laboratory technician, and with an automated device (AD; Clinitek Status, Siemens). The mean concordance for semiquantitative urinalysis results between students and the technician and between students and the AD was fair (κ0.21–0.40) in dogs and cats; concordance was moderate between the technician and the AD (κ0.41–0.60) in dogs and good (κ0.61–0.80) in cats. For pH, the mean concordance between students and the technician and between the technician and the AD was good (ρ0.80–0.92) in dogs and cats; concordance was good between students and the AD (ρ0.80–0.92) in dogs and moderate (ρ0.59–0.79) in cats. Repeatability was higher ( ...

Research paper thumbnail of Spécificités des infections du tractus urinaire chez le chat

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Techniques non instrumentales de désencombrement bronchique chez le chien

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Iconographies supplémentaires de l'article: La leptospirose canine en France: étude rétrospective de 37 cas

HAL (Le Centre pour la Communication Scientifique Directe), 2010

Research paper thumbnail of Comment vos clients utilisent Internet

LYON1-BU Santé (693882101) / SudocSudocFranceF

Research paper thumbnail of Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptospirosis

Revue Vétérinaire Clinique, 2020

Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptos... more Pulmonary dysfunction as a component of a multiple organ dysfunction syndrome in dogs with leptospirosis L'atteinte pulmonaire en tant que composante d'un syndrome de défaillance multiviscérale chez les chiens atteints de leptospirose

Research paper thumbnail of Intérêts et limites de la coproculture dans le diagnostic des diarrhées d'origine bactérienne du chien et du chat

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Research paper thumbnail of Importance et place de la nutrition dans la prise en charge (étude bibliographique et étude clinique retrospective)

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF