Mario Crisantos - Academia.edu (original) (raw)
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Papers by Mario Crisantos
Plant Molecular Biology, 2003
In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recru... more In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of the Zea mays Rpd3-type histone deacetylase (ZmRpd3I) and the retinoblastoma-related (ZmRBR1) homologues were analysed during endosperm development. GST pull-down and immunoprecipitation experiments showed a physical interaction between ZmRBR1 and ZmRpd3I. Because ZmRpd3I lacks a LXCXE motif, conserved in several pRb-interacting proteins, we have mapped the amino acid domains involved in the ZmRBR1/ZmRpd3I interaction. Furthermore, we observed that ZmRbAp1, a maize member of the MSI/RbAp family, facilitated this protein interaction. Co-transformations of tobacco protoplasts with plasmids expressing ZmRBR1 and ZmRpd3I showed that the two proteins cooperate in repressing gene transcription. Our findings represent the first indication that in plants a regulator of important biological processes, ZmRBR1, can recruit a histone deacetylase, ZmRpd3I, to control gene transcription.
Esta enfermedad también conocida como peste maligna, pudrición de la cría, peste viscosa, cría pu... more Esta enfermedad también conocida como peste maligna, pudrición de la cría, peste viscosa, cría putrefacta, etc., es una enfermedad bacteriana infecciosa y altamente contagiosa que afecta a las larvas de las abejas melíferas, causada por el Paenibacillus larvae. Después de la Varroasis es la enfermedad de las abejas que más pérdidas económicas ocasionan en todo el mundo.
Plant Molecular Biology, 2003
In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recru... more In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of the Zea mays Rpd3-type histone deacetylase (ZmRpd3I) and the retinoblastoma-related (ZmRBR1) homologues were analysed during endosperm development. GST pull-down and immunoprecipitation experiments showed a physical interaction between ZmRBR1 and ZmRpd3I. Because ZmRpd3I lacks a LXCXE motif, conserved in several pRb-interacting proteins, we have mapped the amino acid domains involved in the ZmRBR1/ZmRpd3I interaction. Furthermore, we observed that ZmRbAp1, a maize member of the MSI/RbAp family, facilitated this protein interaction. Co-transformations of tobacco protoplasts with plasmids expressing ZmRBR1 and ZmRpd3I showed that the two proteins cooperate in repressing gene transcription. Our findings represent the first indication that in plants a regulator of important biological processes, ZmRBR1, can recruit a histone deacetylase, ZmRpd3I, to control gene transcription.
Esta enfermedad también conocida como peste maligna, pudrición de la cría, peste viscosa, cría pu... more Esta enfermedad también conocida como peste maligna, pudrición de la cría, peste viscosa, cría putrefacta, etc., es una enfermedad bacteriana infecciosa y altamente contagiosa que afecta a las larvas de las abejas melíferas, causada por el Paenibacillus larvae. Después de la Varroasis es la enfermedad de las abejas que más pérdidas económicas ocasionan en todo el mundo.