Mary Varaschin - Academia.edu (original) (raw)

Papers by Mary Varaschin

Natural Toxins, Mar 1, 1996

Ciencia Rural, 2017

Renal cell carcinoma, especially with extensive involvement of peritoneal cavity, is rarely descr... more Renal cell carcinoma, especially with extensive involvement of peritoneal cavity, is rarely described in horses. This study described a case of renal cell carcinoma in an 8-year-old mare, which presented with an enlargement in the left flank, decreased appetite and straining to defecate. Clinically, a tumor in the left retroperitoneal space was detected by rectal examination and ultrasonography. The animal died after one month of clinical evolution. At necropsy, the left kidney was completely effaced by a 50cm in diameter, irregular, round, lobulated cystic mass with necro-hemorrhagic areas. Within the peritoneal cavity, there was severe hemoperitoneum and numerous sessile and pedunculated masses ranging from 2.0 to 15.0cm diameter that were attached to the visceral and parietal peritoneum. Histologically, the primary neoplasia and its metastasis were composed by a proliferation of epithelial cells, which were arranged in a tubulopapillary pattern. Diagnosis of renal cell carcinoma with peritoneal carcinomatosis and hemoperitoneum was based on macroscopic aspects, and mainly on the histological features.

Acta Veterinaria Brasilica, Jun 30, 2017

Article history Skin injuries are frequent in horses, and one of the treatments used for such inj... more Article history Skin injuries are frequent in horses, and one of the treatments used for such injuries is the platelet-rich plasma (PRP). The objective of this experiment was to macroscopically and microscopically investigate the process of healing by second intention in skin wounds of eight healthy gelding crossbred horses treated (T) or untreated (UT) with a single dose of leukocyte-poor PRP (LP-PRP). Three squareshaped wounds were induced in both gluteal regions. After 12 h of wound induction, 0.5 mL LP-PRP was administered in each border of the lesion in one of gluteal region. Contralateral wounds were UT. Six skin biopsies were obtained with a 6-mm Punch. Macroscopic variables of one of the non-biopsied wounds were evaluated. Samples were processed for histomorphometric evaluation. No difference was observed between the time required for wound closure in the two groups. Histomorphometric analysis performed 14 days after wound induction revealed higher (p = 0.034) angiogenesis and number (p = 0.0179) of total leukocytes in T wounds. Fibrocytes numbers increased significantly (p = 0.023) on day 7 after injury in the UT group. General microscopic evaluation performed independently of scores and morphometric analysis revealed that the majority of the T wounds showed better healing variables in the sections analyzed after complete macroscopic closure of the wound. A single injected dose of LP-PRP 12 h after wound induction in horses does not interfere with the wound healing process but reveals that majority of the T wounds exhibit better healing histomorphometric characteristics.

Ciência Animal Brasileira, 2009

Experimental Parasitology, Oct 1, 2019

Goats are frequently described as an intermediate host for the protozoan Neospora caninum, manife... more Goats are frequently described as an intermediate host for the protozoan Neospora caninum, manifesting the disease mainly by recurrent abortions with placentitis and encephalitis in fetuses. Several reports of natural and experimental infections in cattle and mice show differences in the immune response, and the outcome of the infection can be variable depending on the species affected and by the behavior of the infective strain. This study describes for the first time two Neospora caninum strains isolated from naturally infected goats from the state of Minas Gerais, Brazil. One placenta and one brain from different goats were processed for a first bioassay in gerbils (Meriones unguiculatus). Subsequently, a second bioassay was performed by inoculating the processed brain samples from gerbils into Interferon gamma (IFN-γ) knockout mice (KO mice). Tachyzoites collected from the peritoneal fluid of the KO mice were inoculated into VERO cell monolayers, where they presented a very slow growth rate. The tachyzoites were also inoculated into BALB/c mice with a dose of 10 6 tachyzoites per animal. After a 5-week follow up, the animals infected with both of the strains developed a strong polarized Th1 response with increased serum and spleen gene expression levels of pro-inflammatory cytokines (mainly IFN-γ and TNF-α) in the first week. Tissue lesions were mild in the animals infected with both strains. Despite the strong immune response preventing an infection in the visceral organs, the parasite was able to reach the brain, causing progressive brain lesions from the second to fifth week post infection. The NC-goat1-infected mice presented with severe meningoencephalitis, but the NC-goat2-infected animals had considerable histological brain lesions only at week 5. Immunohistochemical analysis of the mouse brains revealed a different pattern of inflammatory cells compared to the naturally infected goats. A severe inflammatory infiltrate of CD3 + T lymphocytes was found in the NC-goat1-infected mice. A more discrete infiltrate of CD3 + T cells was found in the NC-goat2-infected animals. Additionally, IBA1 IHC revealed an intense microglial reaction and monocyte perivascular cuffs in the NC-goat1-infected animals and lower microglia/monocyte infiltrates in the NC-goat2infected mice. This work contributes knowledge on the pathogenicity of new Neospora caninum strains in mice, comparable with other well-established mouse models of the disease, and demonstrates the importance of studying goats as an intermediate host of this parasite.

An outbreak of diarrhea in adult cattle from a dairy herd of Lavras county, MG, was described. Fr... more An outbreak of diarrhea in adult cattle from a dairy herd of Lavras county, MG, was described. From a herd of 10 cows, seven became ill. The diarrhea lasted six to 10 days, being more prolonged, intense, and with blood in lacting cows. Other signs included apathy, reduced appetite and milk production, and serous nasolacrimal discharge. Bovine coronavirus was detected in fecal samples from the five cattle tested by nested-RT-PCR.

PubMed, Sep 1, 2012

The present study describes the use of a microwave processing protocol for the rapid histopatholo... more The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.

Small Ruminant Research, Jul 1, 2016

Abstract Histopathology, immunohistochemistry (IHC) and molecular biology methods were used to ev... more Abstract Histopathology, immunohistochemistry (IHC) and molecular biology methods were used to evaluate the lesions and the distribution of Neospora caninum in the tissues of eight female non-pregnant goats, naturally infected with N. caninum. The goats transmitted the parasite to their offspring in previous pregnancies. Histopathological examination showed a mild lymphoplasmacytic inflammatory infiltrate in skeletal muscles (10/168 muscles; 1.68%) and myocardium in two goats. Moderate to severe lymphoplasmacytic perivascular cuffs were the most significant lesion in the nervous system and were observed in 62.5% of the goats. Mild to moderate gliosis also affected 37.5% of them. Parasitic structures were not found in the tissues by histopathology and IHC labeling for N. caninum Polymerase chain reaction (PCR) analysis of 359 tissue samples showed 133 (37%) positive samples. The highest percentage of positive results was found in the nervous system (28/63; 44.4%) and skeletal muscle (72/168; 42.8%). Statistical analysis of the PCR results did not show parasitic preference for any particular muscle group (head, hindlimbs, neck, thoracoabdominal or forelimb muscles) or region of the nervous system. Yet, the highest percentage of PCR-positive samples was from the cleido-occipital, brachial triceps and semimembranosus muscles and the cervical segment of the spinal cord (62.5% each).These data suggest that PCR diagnosis of N. caninum infection should include analysis of these tissues. This is the first report of N. caninum distribution in adult female goat tissues.

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Aug 1, 2009

Ciência Animal Brasileira, Oct 21, 2009

Revista Brasileira de Saúde e Produção Animal, Jun 1, 2017

The objective of this study was to evaluate the performance, carcass, meat quality, fatty acid pr... more The objective of this study was to evaluate the performance, carcass, meat quality, fatty acid profile and liver alterations of cattle supplemented with cottonseed feedlot. It used 100 Nelore cattle, being divided into two groups of 50 animals, which received a cottonseed (CS) in grain form by the diet in the proportion of 15% and the other group was provided with control diet without CS in the feedlot for 88 days. The diets were isoprotein and isoenergetic and concentrate:roughage ratio of 60:40. The animals were slaughter in the end of experimental time and the collection of liver samples for histological analysis. After cooling 24 hours at ± 1°C were conducted evaluation of carcass and major cuts, final pH and collected muscle samples longissimus to carry out the physicochemical analyzes (Color, Cooking Loss, Pigments and Shear Force), chemical composition, fatty acid profile and oxidation. The inclusion of CS in the diet provided animals with higher slaughter weight, daily weight gain, Hindquarter, however, the animals showed reduced carcass yield, forequarter weight and Thin flank, without changing the parameters as rib-eye area and backfat thickness. There was an increase of the yellow values (b*) and moisture with the final pH reduction. There was no influence of the use of cottonseed in relation to histological parameters evaluated in the liver. The cottonseed in the feeding feedlot cattle promoted improvement in animal performance, despite the reduction of the forequarter weight, with appropriate aspects of meat quality, fatty acid profile and conservation.

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Dec 1, 2006

Relata-se o caso de um cão, sem raça definida, levado ao veterinário por apresentar cansaço fácil... more Relata-se o caso de um cão, sem raça definida, levado ao veterinário por apresentar cansaço fácil e episódios de síncope. Foram identificadas fibrilação atrial e extra-sístoles ventriculares isoladas ao exame eletrocardiográfico. O tratamento antiarrítmico não foi eficaz, e o animal morreu devido a um episódio de síncope. À necropsia, observou-se massa em região de base cardíaca, invadindo a luz do átrio esquerdo. Histologicamente, constatou-se a presença de quimiodectoma. A infiltração miocárdica atrial causou lesões nos miócitos, produzindo circuitos reentrantes e/ou focos ectópicos de despolarização atrial.

Pesquisa Veterinária Brasileira, 2018

ABSTRACT: Crab-eating foxes (Cerdocyon thous) are wild canids found in practically all Brazilian ... more ABSTRACT: Crab-eating foxes (Cerdocyon thous) are wild canids found in practically all Brazilian states. They usually live and hunt in pairs, but can be found in small groups, which favors the transmission of diseases such as sarcoptic mange. This study aims to describe the epidemiological, parasitological and pathological findings of two fatal cases of sarcoptic mange in C. thous. Two wild canids were necropsied and cytological examination of skin and crust samples and histologic evaluation of various tissue samples were performed. Gross findings included poor body condition and extensive alopecia with thick skin crusts interspersed by intensely reddened alopecic areas. The cytological examination revealed numerous mites with short gnathosoma and rounded idiosoma that were transversally striated and presented triangular spines on the dorsal surface, terminal anus, and short and thick legs, characteristic of Sarcoptes scabiei. Histologic examination of the skin revealed numerous tun...

Pesquisa Veterinaria Brasileira, Mar 1, 2018

RESUMO.-[Lesões placentárias associadas a abortos e natimortos em cabras naturalmente infectadas ... more RESUMO.-[Lesões placentárias associadas a abortos e natimortos em cabras naturalmente infectadas pelo Neospora caninum.] Neospora caninum é descrito como um parasito que causa problemas reprodutivos esporádicos em cabras. Muitos aspectos da patogênese da neosporose

Journal of NeuroVirology, Jun 10, 2014

Pesquisa Veterinaria Brasileira, Jun 1, 2012

RESUMO.-Um surto de compactação primária de abomaso associada ao consumo de silagem de girassol o... more RESUMO.-Um surto de compactação primária de abomaso associada ao consumo de silagem de girassol ocorreu num rebanho bovino leiteiro do estado de Minas Gerais, do qual seis de 21 vacas secas em fase ϐinal de gestação e que recebiam silagem de girassol morreram. Os bovinos que adoeceram apresentaram distensão abdominal, fezes ressecadas e escassas e, nos bovinos que morreram, eram pastosas, escuras e fétidas; nesses havia também desidratação acentuada e palidez de mucosas. Três bovinos foram necropsiados e os achados patológicos eram constituídos principalmente por distensão acentuada do abomaso associada a grande quantidade de conteúdo alimentar ressecado coberto por coágulos de sangue. No abomaso havia úlceras, com perfuração da parede em um dos bovinos. A

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Feb 1, 2011

A glândula adrenal é composta por duas regiões distintas: o córtex e a medular. Um dos componente... more A glândula adrenal é composta por duas regiões distintas: o córtex e a medular. Um dos componentes principais da região medular são as células cromafins, as quais são neurônios simpáticos modificados que sintetizam epinefrina e norepinefrina distintamente (Hullinger e Andrisani, 2006).

Veterinária notícias, Sep 21, 2015

Open Journal of Veterinary Medicine, 2018

This report describes a case of cardiomyopathy associated with excessive moderator bands in a 7 y... more This report describes a case of cardiomyopathy associated with excessive moderator bands in a 7 years old cat. In the cat echocardiography examination was observed cardiomegaly, decreased systolic function and thinning of the left ventricle wall. The electrocardiogram showed the occurrence of supraventricular tachycardia. The treatment was initiated with digoxin, furosemide and taurine supplementation, with an improvement of the clinical signs, however the animal died in a fall. The diagnosis of cardiomyopathy associated with excessive moderator band, was based on the multiple cardiac bunds which connect the septum to the free wall of the left ventricle observed in the cat necropsy.

Epidemiology and Infection, Feb 18, 2013

This study was conducted to determine the seroprevalence of anti-Neospora caninum antibodies and ... more This study was conducted to determine the seroprevalence of anti-Neospora caninum antibodies and to investigate the risk factors related to seroprevalence in dogs from urban and rural areas with distinct economic activities (milk and coffee production) in Minas Gerais state, Brazil. For this purpose, blood samples from 703 dogs were collected and questionnaires addressing epidemiological aspects were completed by dog-owners. The sera were analysed for anti-N. caninum antibodies by indirect fluorescent antibody tests (IFAT 5 1:50). Association between epidemiological aspects and seropositivity in dogs was evaluated with multivariate logistic regression models. A total of 80 (11•4%) dogs tested positive for N. caninum. In the multivariate logistic regression models, dogs aged >4 years, dogs used as guard dogs, dogs that spontaneously hunt, and history of bovine abortion were found to be greater risk factors for canine N. caninum infection. When we considered only dogs from rural areas, an association with seroprevalence was seen for milk farms, dogs not fed with commercial food, dogs that hunt, and dogs used as guard dogs.