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Papers by Marylène Poirié

Box-plot representations of the THC (A) and the proportion of granulocytes (B), estimate... more Box-plot representations of the THC (A) and the proportion of granulocytes (B), estimated on F-actin stained AHPs (pools of 5 adults per AHP), for aphid lines listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042114#pone-0042114-t001" target="_blank">Table 1</a>. Box-plots with the same letter have means that are not significantly different (TukeyHSD, alpha = 5e-03, n = 4).</p

Entomologia Generalis, 2022

Bulletin of Insectology, 2017

We report here the first observation of Zaprionus indianus Gupta in mainland France. Five specime... more We report here the first observation of Zaprionus indianus Gupta in mainland France. Five specimens of both sexes were trapped in a liquid vinegar bait in Cap d’Antibes (French Riviera) and their taxonomic identification was then performed by morphologi-cal and molecular analysis. Z. indianus is recognized as a fig pest in the invaded American countries, and it could pose economic problems in this new location since figs are also grown in European Mediterranean areas. This Drosophilid species is on the EPPO pest quarantine list. Its spread and possible permanent establishment in South France should thus be monitored in the next years to assess possible agricultural damages.

PLOS ONE, 2018

Drosophila suzukii (the spotted-wing Drosophila) appears to be unsuitable for the development of ... more Drosophila suzukii (the spotted-wing Drosophila) appears to be unsuitable for the development of most Drosophila larval endoparasitoids, be they sympatric or not. Here, we questioned the physiological bases of this widespread failure by characterizing the interactions between D. suzukii and various parasitoid species (Asobara japonica, Leptopilina boulardi, Leptopilina heterotoma and Leptopilina victoriae) and comparing them with those observed with D. melanogaster, a rather appropriate host. All parasitoids were able to oviposit in L1 and L2 larval stages of both hosts but their propensity to parasitize was higher on D. melanogaster. A. japonica and, to a much lesser extent, L. heterotoma, were the two species able to successfully develop in D. suzukii, the failure of the parasitism resulting either in the parasitoid encapsulation (notably with L. heterotoma) or the host and parasitoid deaths (especially with L. boulardi and L. victoriae). Compared to D. melanogaster, encapsulation in D. suzukii was strongly delayed and led, if successful, to the production of much larger capsules in surviving flies and, in the event of failure, to the death of both partners because of an uncontrolled melanization. The results thus revealed a different timing of the immune response to parasitoids in D. suzukii compared to D. melanogaster with a lose-lose outcome for parasitoids (generally unsuccessful development) and hosts (high mortality and possible reduction of the fitness of survivors). Finally, these results might suggest that some European endoparasitoids of Drosophila interact with this pest in the field in an unmeasurable way, since they kill their host without reproductive success.

Biopesticides based onBacillus thuringiensis(Bt) spores and toxins are alternate pest management ... more Biopesticides based onBacillus thuringiensis(Bt) spores and toxins are alternate pest management solutions widely used to control insect pests. Their increasing use could lead to accumulation in the environment, hence leading to chronic exposure of non-target organisms. Here, we tested for potential non-intentional side-effects of chronic exposure toBtbiopesticide on larvae of non-targetDrosophilaspecies present inBt-treated areas. Doses up to those recommended for field application (106CFU/g of fly medium) had no effect on the fly development, whereas doses 10 to 100-fold higher (107-108CFU/g) increased developmental time and decreased adult emergence rates in a dose-dependent manner and with varying effect amplitudes for all the species and strains tested. For all them, all larvae died before pupation at the highest dose tested (109CFU/g). Focusing onD. melanogaster, delayed development and reduced emergence resulted from stage-dependent larval mortality, and fitness-related trait...

Molecular ecology resources, Jan 18, 2015

Understanding the forces that shape eco-evolutionary patterns often requires linking phenotypes t... more Understanding the forces that shape eco-evolutionary patterns often requires linking phenotypes to genotypes, allowing characterization of these patterns at the molecular level. DNA-based markers are less informative in this aim compared to markers associated with gene expression and, more specifically, with protein quantities. The characterization of eco-evolutionary patterns also usually requires the analysis of large sample sizes to accurately estimate interindividual variability. However, the methods used to characterize and compare protein samples are generally expensive and time-consuming, which constrains the size of the produced data sets to few individuals. We present here a method that estimates the interindividual variability of protein quantities based on a global, semi-automatic analysis of 1D electrophoretic profiles, opening the way to rapid analysis and comparison of hundreds of individuals. The main original features of the method are the in silico normalization of ...

Journal of Biological Chemistry, 2011

Genome Research, 1999

Drosophila melanogaster larvae usually react against eggs of the parasitoid wasp Leptopilina boul... more Drosophila melanogaster larvae usually react against eggs of the parasitoid wasp Leptopilina boulardi by surrounding them with a multicellular melanotic capsule. The genetic determinism of this response has been studied previously using susceptible (non-capsule-forming) and resistant (capsule-forming) strains. The results suggest that differences in their encapsulation response involve a single gene, resistance to Leptopilina boulardi(Rlb), with two alleles, the resistant one being dominant.Rlb confers specific protection against Leptopilina boulardi and is thus probably involved in parasitoid recognition. Recent studies have localized this gene on the right arm of the second chromosome and our aim was to precisely determine its genetic and molecular location. Using strains bearing deletions, we demonstrated that resistance to Leptopilina boulardi is conferred by the55C; 55F3 region and that the 55E2–E6; F3 region is particularly involved. A physical map of the 55C;56A region was th...

Additional file 12. OXPHOS gene annotations. Details of annotated OXPHOS genes, including duplica... more Additional file 12. OXPHOS gene annotations. Details of annotated OXPHOS genes, including duplications in the assembly.

Authors' original file for figure 3

Additional file 4:Amino acid sequence alignment of Imaginal disc Growth Factors (IDGFs)-like prot... more Additional file 4:Amino acid sequence alignment of Imaginal disc Growth Factors (IDGFs)-like proteins from different insect species. The sequence of the Ci-48b from C. inanitus was aligned with sequences from the following species: N. vitripennis [GenBank:XP_001599305.1], A. mellifera [GenBank:XP_396769.2] and Manduca sexta [GenBank:ACW82749.1]. The conserved region II is boxed. Triangle indicates a glutamine residue replacing, in these proteins, a glutamic acid residue of functional importance. Location of the glycosyl hydrolase family 18 domain is indicated by a blue line. Red and green arrows indicate the beginning of the predicted signal peptide and mature protein sequences of Ci-48b, respectively. Sequence printed in bold was also obtained by N-terminal sequencing of the Ci-48b protein. (PNG 274 KB)

Additional file of The venom composition of the parasitic wasp Chelonus inanitus resolved by comb... more Additional file of The venom composition of the parasitic wasp Chelonus inanitus resolved by combined expressed sequence tags analysis and proteomic approach

Additional file 1:Table of peptide identification. Peptidic sequences obtained from the nano-LC-M... more Additional file 1:Table of peptide identification. Peptidic sequences obtained from the nano-LC-MS/MS analysis of venom proteins from C. inanitus are shown in blue into the corresponding amino acid sequences encoded by ORFs obtained from the transcriptome analysis of C. inanitus venom glands. (XLS 16 KB)

Additional file 9. Venom gene annotations. Annotation details of venom genes in L. fabarum and A.... more Additional file 9. Venom gene annotations. Annotation details of venom genes in L. fabarum and A. ervi.

Additional file 8. Ortholog MAtrix (OMA) results. Summary of all Hierarchical Ortholog Groups (HO... more Additional file 8. Ortholog MAtrix (OMA) results. Summary of all Hierarchical Ortholog Groups (HOGs) predicted by OMA, including details of LRR genes.

Additional file 2. Contamination filtering for L. fabarum. Details of both blast-based and blobto... more Additional file 2. Contamination filtering for L. fabarum. Details of both blast-based and blobtools contamination filtering performed on the L. fabarum genome.

Additional file 3. Linkage groups in L. fabarum. Details of scaffold positions used to construct ... more Additional file 3. Linkage groups in L. fabarum. Details of scaffold positions used to construct linkage groups for L. fabarum.

Data files for the publication "Rapid and Differential Evolution of the Venom Composition of... more Data files for the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain." ******************************* ****** GLOBAL ANALYSIS ******************************* The compressed folder "./exemple_gel_1.zip" is an example of analysis of a gel done with CLIQS (http://totallab.com/home/cliqs/). "img_7061_converted.tif" is the analysed picture. "IMG_7061.1dxml" is the CLIQS database containing the position of the lanes, the Rf lines and the "grimaces". For details see (1). "export_without_bg" and "export_with_bg" are containing the intensity profiles exported by CLIQS and used as input for the R-functions published in (1). The file "./global_analysis_nobg_H_Q.txt" is the output of the the R-functions published in (1). This is t...

Entomologia Generalis, 2022

Bulletin of Insectology, 2017

PLOS ONE, 2018

Molecular ecology resources, Jan 18, 2015

Journal of Biological Chemistry, 2011

Genome Research, 1999

Authors' original file for figure 3