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Papers by Marzieh Ghassemi

Research paper thumbnail of Evaluation of the Entomopathogenic Fungus Beauveria bassiana on Different Stages of Phlebotomus papatasi (Diptera: Psychodidae), Vector of Zoonotic Cutaneous Leishmaniasis in Iran

Journal of arthropod-borne diseases, Feb 24, 2024

Research paper thumbnail of Report of the Crimean Congo hemorrhagic fever in Abadan, Iran: Summer 2016 (Case Report)

علوم بهداشتی ایران, Jan 11, 2020

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitte... more Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitted through person-to-person transmission by exposure to infected body fluids. It causes a severe disease in humans with high mortality rates. Here we present two cases of CCHF patients with sudden onset of lethargy, fever, nausea, vomiting, headache, and hemorrhagic manifestation. With one of the patients tick bite was confirmed, then he was treated with ribavirin in isolation and recovered completely. The second patient was in contact with the infected blood of a sheep and, unfortunately, despite the treatment, he passed away. Public health measures should focus on preventing this infection by raising the awareness of CCHF symptoms and route of its transmission, and also by adopting practices to decrease the chances of spreading infections in hospitals.

Research paper thumbnail of Molecular and Biochemical Detection of Insecticide Resistance in the <i>Leishmania</i> Vector, <i>Phlebotomus papatasi</i> (Diptera: Psychodidae) to Dichlorodiphenyltrichloroethane and Pyrethroids, in Central Iran

Journal of Medical Entomology, May 20, 2022

The aim of the present study was to explore resistance markers and possible biochemical resistanc... more The aim of the present study was to explore resistance markers and possible biochemical resistance mechanisms in the Phlebotomine sand fly Phlebotomus papatasi in Esfahan Province, central Iran. Homogenous resistant strains of sand flies were obtained by exposing P. papatasi collected from Esfahan to a single diagnostic dose of DDT. The adults from the colony were tested with papers impregnated with four pyrethroid insecticides: Permethrin 0.75%, Deltamethrin 0.05%, Cyfluthrin 0.15%, and Lambdacyhalothrin 0.05% to determine levels of cross-resistance. To discover the presence of mutations, a 440 base pair fragment of the voltage gated sodium channel (VGSC) gene was amplified and sequenced in both directions for the susceptible and resistant colonies. We also assayed the amount of four enzymes that play a key role in insecticide detoxification in the resistant colonies. A resistance ratio (RR) of 2.52 folds was achieved during the selection of resistant strains. Sequence analysis revealed no knockdown resistance (kdr) mutations in the VGSC gene. Enzyme activity ratio of the resistant candidate and susceptible colonies were calculated for α-esterases (3.78), β-esterases (3.72), mixed function oxidases (MFO) (3.21), and glutathione-S-transferases (GST) (1.59). No cross-resistance to the four pyrethroids insecticides was observed in the DDT resistant colony. The absence of kdr mutations in the VGSC gene suggests that alterations in esterase and MFO enzymes are responsible for the resistant of P. papatasi to DDT in central Iran. This information could have significant predictive utility in managing insecticide resistant in this Leishmania vector.

Research paper thumbnail of Prevalence of malnutrition and its related factors among urban and rural primary school students, Abadan, in 2019

Asian journal of social health and behavior, 2022

Research paper thumbnail of Rodents as vehicle for delivery of transgenic bacteria to make paratransgenic sand fly vectors of cutaneous leishmaniasis in field condition

Scientific Reports

Vector-borne diseases, among them leishmaniasis, cause more than 700,000 deaths annually. The lac... more Vector-borne diseases, among them leishmaniasis, cause more than 700,000 deaths annually. The lack of an effective vaccination and the increasing resistance of sand flies to insecticides require the urgent development of innovative approaches to contain the disease. The use of engineered bacteria that express anti-parasite molecules (paratransgenesis) shows much promise. However, a challenge for implementation of this strategy is to devise means to introduce modified bacteria into sand flies in the field. In this study, we use rodent food bait as a delivery strategy to introduce two mCherry-fluorescent bacteria, Serratia AS1 and Enterobacter cloacae, into adult sand flies in field settings. Bacteria-infected food was provided to Rhombomys opimus rodents. These bacteria transiently pass through the rodent alimentary tract and are delivered to larval habitats with the rodent feces. The feces are ingested by sand fly larvae and, in the case of Serratia AS1, are trans-stadially transmit...

Research paper thumbnail of Prevalence of malnutrition and its related factors among urban and rural primary school students, Abadan, in 2019

Asian Journal of Social Health and Behavior

Research paper thumbnail of Determination of Vitamins B Group Concentrations in Dorema Aucheri by HPLC-Uv

Solid phase extraction (SPE) was proposed for simultaneous extraction of trace levels of vitamins... more Solid phase extraction (SPE) was proposed for simultaneous extraction of trace levels of vitamins B1, B2, B3, B5, B6, B7, B9 and B12. SPE method was proposed for determination of vitamins B in Dorema aucheri. High performance liquid chromatography with UV detector (HPLC-UV) was employed to analysis the vitamins. The affecting parameters in chromatographic conditions such as, pH, ratios of the solvents and temperature were studied. Under the optimal conditions, relative standard deviations (RSD) of the analysis less than 11% (n= 3) and detection limit were detected less than 1 ng kg -1 for vitamins. The proposed method was successfully applied for the determination of vitamins B in Dorema aucheri.

Research paper thumbnail of Simultaneous Determination Trace Levels of Vitamin B1 and Vitamin B9 in Human Samples by Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Coupled with HPLC-Uv

Ultrasound-assisted dispersive liquid-liquid micro extraction (UA-DLLME) was proposed for simulta... more Ultrasound-assisted dispersive liquid-liquid micro extraction (UA-DLLME) was proposed for simultaneous determination of trace levels of Vitamin B1 (thiamine) and Vitamin B9 (folic acid) in the human urine and serum samples. High performance liquid chromatography (HPLC) coupled with chemometrics method was employed to analysis the extraxtants. Objective: The optimal conditions of the extraction recovery, including pH of sample solution, type and volume of extraction solvent, disperser solvent type and volume, temperature and time of ultrasound, centrifugation time and ionic strength were were studied step-by-step via one-factor-at-a time procedure. Then significant variables were chosen and considered to further optimize using the hybrid Box–Behnken design -genetic algorithm. UADLLME method with HPLC-UV and utilizing hybrid Box–Behnken design genetic algorithm to optimize the extraction process was presented for the first time in this study. Under the optimized extractions, relative ...

Research paper thumbnail of Implementation Status of Moral Codes among Nurses

World Family Medicine Journal/Middle East Journal of Family Medicine, 2017

Background and objective: Nurses should have an appropriate level of ethical development to perfo... more Background and objective: Nurses should have an appropriate level of ethical development to perform their daily care. Ethical codes should be understood by nurses and used in each dimension of nursing practice. Therefore, this study aimed to investigate the implementation status of nurses' ethical codes. Methods: This descriptive-analytic study was carried out on 202 nurses working in internal and surgical wards using easy sampling in educational hospitals of the Faculty of Medical Sciences, Abadan. The implementation status of ethical codes was investigated using a researcher-made questionnaire and the obtained data were analyzed using SPSS ver. 16. Results: There was a statistically significant difference between the level of ethical performance of nurses with cases such as organizational positions, work shift, academic education, and ethics retraining courses and work experience (p< 0.005). This difference was not observed in relation to gender, marital status, educational degree, history of presenting complaints and job satisfaction and the workplace (p>0.005). Conclusion: Results of this study showed that nurses should firstly recognize the dimensions and ethical issues in their profession for the ethical performance of professional nursing; therefore, it is recommended to maximize the efficiency and quality of health care by educating the medical staff and raising their awareness of professional ethics.

Research paper thumbnail of Molecular and Biochemical Detection of Insecticide Resistance in the Leishmania Vector, Phlebotomus papatasi (Diptera: Psychodidae) to Dichlorodiphenyltrichloroethane and Pyrethroids, in Central Iran

Journal of Medical Entomology

The aim of the present study was to explore resistance markers and possible biochemical resistanc... more The aim of the present study was to explore resistance markers and possible biochemical resistance mechanisms in the Phlebotomine sand fly Phlebotomus papatasi in Esfahan Province, central Iran. Homogenous resistant strains of sand flies were obtained by exposing P. papatasi collected from Esfahan to a single diagnostic dose of DDT. The adults from the colony were tested with papers impregnated with four pyrethroid insecticides: Permethrin 0.75%, Deltamethrin 0.05%, Cyfluthrin 0.15%, and Lambdacyhalothrin 0.05% to determine levels of cross-resistance. To discover the presence of mutations, a 440 base pair fragment of the voltage gated sodium channel (VGSC) gene was amplified and sequenced in both directions for the susceptible and resistant colonies. We also assayed the amount of four enzymes that play a key role in insecticide detoxification in the resistant colonies. A resistance ratio (RR) of 2.52 folds was achieved during the selection of resistant strains. Sequence analysis rev...

Research paper thumbnail of Report of the Crimean Congo hemorrhagic fever in Abadan, Iran: Summer 2016 (Case Report)

Iranian Journal of Health Sciences

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitte... more Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitted through person-to-person transmission by exposure to infected body fluids. It causes a severe disease in humans with high mortality rates. Here we present two cases of CCHF patients with sudden onset of lethargy, fever, nausea, vomiting, headache, and hemorrhagic manifestation. With one of the patients tick bite was confirmed, then he was treated with ribavirin in isolation and recovered completely. The second patient was in contact with the infected blood of a sheep and, unfortunately, despite the treatment, he passed away. Public health measures should focus on preventing this infection by raising the awareness of CCHF symptoms and route of its transmission, and also by adopting practices to decrease the chances of spreading infections in hospitals.

Research paper thumbnail of Evaluation of the Entomopathogenic Fungus Beauveria bassiana on Different Stages of Phlebotomus papatasi (Diptera: Psychodidae), Vector of Zoonotic Cutaneous Leishmaniasis in Iran

Journal of arthropod-borne diseases, Feb 24, 2024

Research paper thumbnail of Report of the Crimean Congo hemorrhagic fever in Abadan, Iran: Summer 2016 (Case Report)

علوم بهداشتی ایران, Jan 11, 2020

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitte... more Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitted through person-to-person transmission by exposure to infected body fluids. It causes a severe disease in humans with high mortality rates. Here we present two cases of CCHF patients with sudden onset of lethargy, fever, nausea, vomiting, headache, and hemorrhagic manifestation. With one of the patients tick bite was confirmed, then he was treated with ribavirin in isolation and recovered completely. The second patient was in contact with the infected blood of a sheep and, unfortunately, despite the treatment, he passed away. Public health measures should focus on preventing this infection by raising the awareness of CCHF symptoms and route of its transmission, and also by adopting practices to decrease the chances of spreading infections in hospitals.

Research paper thumbnail of Molecular and Biochemical Detection of Insecticide Resistance in the <i>Leishmania</i> Vector, <i>Phlebotomus papatasi</i> (Diptera: Psychodidae) to Dichlorodiphenyltrichloroethane and Pyrethroids, in Central Iran

Journal of Medical Entomology, May 20, 2022

The aim of the present study was to explore resistance markers and possible biochemical resistanc... more The aim of the present study was to explore resistance markers and possible biochemical resistance mechanisms in the Phlebotomine sand fly Phlebotomus papatasi in Esfahan Province, central Iran. Homogenous resistant strains of sand flies were obtained by exposing P. papatasi collected from Esfahan to a single diagnostic dose of DDT. The adults from the colony were tested with papers impregnated with four pyrethroid insecticides: Permethrin 0.75%, Deltamethrin 0.05%, Cyfluthrin 0.15%, and Lambdacyhalothrin 0.05% to determine levels of cross-resistance. To discover the presence of mutations, a 440 base pair fragment of the voltage gated sodium channel (VGSC) gene was amplified and sequenced in both directions for the susceptible and resistant colonies. We also assayed the amount of four enzymes that play a key role in insecticide detoxification in the resistant colonies. A resistance ratio (RR) of 2.52 folds was achieved during the selection of resistant strains. Sequence analysis revealed no knockdown resistance (kdr) mutations in the VGSC gene. Enzyme activity ratio of the resistant candidate and susceptible colonies were calculated for α-esterases (3.78), β-esterases (3.72), mixed function oxidases (MFO) (3.21), and glutathione-S-transferases (GST) (1.59). No cross-resistance to the four pyrethroids insecticides was observed in the DDT resistant colony. The absence of kdr mutations in the VGSC gene suggests that alterations in esterase and MFO enzymes are responsible for the resistant of P. papatasi to DDT in central Iran. This information could have significant predictive utility in managing insecticide resistant in this Leishmania vector.

Research paper thumbnail of Prevalence of malnutrition and its related factors among urban and rural primary school students, Abadan, in 2019

Asian journal of social health and behavior, 2022

Research paper thumbnail of Rodents as vehicle for delivery of transgenic bacteria to make paratransgenic sand fly vectors of cutaneous leishmaniasis in field condition

Scientific Reports

Vector-borne diseases, among them leishmaniasis, cause more than 700,000 deaths annually. The lac... more Vector-borne diseases, among them leishmaniasis, cause more than 700,000 deaths annually. The lack of an effective vaccination and the increasing resistance of sand flies to insecticides require the urgent development of innovative approaches to contain the disease. The use of engineered bacteria that express anti-parasite molecules (paratransgenesis) shows much promise. However, a challenge for implementation of this strategy is to devise means to introduce modified bacteria into sand flies in the field. In this study, we use rodent food bait as a delivery strategy to introduce two mCherry-fluorescent bacteria, Serratia AS1 and Enterobacter cloacae, into adult sand flies in field settings. Bacteria-infected food was provided to Rhombomys opimus rodents. These bacteria transiently pass through the rodent alimentary tract and are delivered to larval habitats with the rodent feces. The feces are ingested by sand fly larvae and, in the case of Serratia AS1, are trans-stadially transmit...

Research paper thumbnail of Prevalence of malnutrition and its related factors among urban and rural primary school students, Abadan, in 2019

Asian Journal of Social Health and Behavior

Research paper thumbnail of Determination of Vitamins B Group Concentrations in Dorema Aucheri by HPLC-Uv

Solid phase extraction (SPE) was proposed for simultaneous extraction of trace levels of vitamins... more Solid phase extraction (SPE) was proposed for simultaneous extraction of trace levels of vitamins B1, B2, B3, B5, B6, B7, B9 and B12. SPE method was proposed for determination of vitamins B in Dorema aucheri. High performance liquid chromatography with UV detector (HPLC-UV) was employed to analysis the vitamins. The affecting parameters in chromatographic conditions such as, pH, ratios of the solvents and temperature were studied. Under the optimal conditions, relative standard deviations (RSD) of the analysis less than 11% (n= 3) and detection limit were detected less than 1 ng kg -1 for vitamins. The proposed method was successfully applied for the determination of vitamins B in Dorema aucheri.

Research paper thumbnail of Simultaneous Determination Trace Levels of Vitamin B1 and Vitamin B9 in Human Samples by Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Coupled with HPLC-Uv

Ultrasound-assisted dispersive liquid-liquid micro extraction (UA-DLLME) was proposed for simulta... more Ultrasound-assisted dispersive liquid-liquid micro extraction (UA-DLLME) was proposed for simultaneous determination of trace levels of Vitamin B1 (thiamine) and Vitamin B9 (folic acid) in the human urine and serum samples. High performance liquid chromatography (HPLC) coupled with chemometrics method was employed to analysis the extraxtants. Objective: The optimal conditions of the extraction recovery, including pH of sample solution, type and volume of extraction solvent, disperser solvent type and volume, temperature and time of ultrasound, centrifugation time and ionic strength were were studied step-by-step via one-factor-at-a time procedure. Then significant variables were chosen and considered to further optimize using the hybrid Box–Behnken design -genetic algorithm. UADLLME method with HPLC-UV and utilizing hybrid Box–Behnken design genetic algorithm to optimize the extraction process was presented for the first time in this study. Under the optimized extractions, relative ...

Research paper thumbnail of Implementation Status of Moral Codes among Nurses

World Family Medicine Journal/Middle East Journal of Family Medicine, 2017

Background and objective: Nurses should have an appropriate level of ethical development to perfo... more Background and objective: Nurses should have an appropriate level of ethical development to perform their daily care. Ethical codes should be understood by nurses and used in each dimension of nursing practice. Therefore, this study aimed to investigate the implementation status of nurses' ethical codes. Methods: This descriptive-analytic study was carried out on 202 nurses working in internal and surgical wards using easy sampling in educational hospitals of the Faculty of Medical Sciences, Abadan. The implementation status of ethical codes was investigated using a researcher-made questionnaire and the obtained data were analyzed using SPSS ver. 16. Results: There was a statistically significant difference between the level of ethical performance of nurses with cases such as organizational positions, work shift, academic education, and ethics retraining courses and work experience (p< 0.005). This difference was not observed in relation to gender, marital status, educational degree, history of presenting complaints and job satisfaction and the workplace (p>0.005). Conclusion: Results of this study showed that nurses should firstly recognize the dimensions and ethical issues in their profession for the ethical performance of professional nursing; therefore, it is recommended to maximize the efficiency and quality of health care by educating the medical staff and raising their awareness of professional ethics.

Research paper thumbnail of Molecular and Biochemical Detection of Insecticide Resistance in the Leishmania Vector, Phlebotomus papatasi (Diptera: Psychodidae) to Dichlorodiphenyltrichloroethane and Pyrethroids, in Central Iran

Journal of Medical Entomology

The aim of the present study was to explore resistance markers and possible biochemical resistanc... more The aim of the present study was to explore resistance markers and possible biochemical resistance mechanisms in the Phlebotomine sand fly Phlebotomus papatasi in Esfahan Province, central Iran. Homogenous resistant strains of sand flies were obtained by exposing P. papatasi collected from Esfahan to a single diagnostic dose of DDT. The adults from the colony were tested with papers impregnated with four pyrethroid insecticides: Permethrin 0.75%, Deltamethrin 0.05%, Cyfluthrin 0.15%, and Lambdacyhalothrin 0.05% to determine levels of cross-resistance. To discover the presence of mutations, a 440 base pair fragment of the voltage gated sodium channel (VGSC) gene was amplified and sequenced in both directions for the susceptible and resistant colonies. We also assayed the amount of four enzymes that play a key role in insecticide detoxification in the resistant colonies. A resistance ratio (RR) of 2.52 folds was achieved during the selection of resistant strains. Sequence analysis rev...

Research paper thumbnail of Report of the Crimean Congo hemorrhagic fever in Abadan, Iran: Summer 2016 (Case Report)

Iranian Journal of Health Sciences

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitte... more Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne, viral disease that may also be transmitted through person-to-person transmission by exposure to infected body fluids. It causes a severe disease in humans with high mortality rates. Here we present two cases of CCHF patients with sudden onset of lethargy, fever, nausea, vomiting, headache, and hemorrhagic manifestation. With one of the patients tick bite was confirmed, then he was treated with ribavirin in isolation and recovered completely. The second patient was in contact with the infected blood of a sheep and, unfortunately, despite the treatment, he passed away. Public health measures should focus on preventing this infection by raising the awareness of CCHF symptoms and route of its transmission, and also by adopting practices to decrease the chances of spreading infections in hospitals.