Masahiro Mii - Academia.edu (original) (raw)

Papers by Masahiro Mii

Plant Production Science, 2002

Abstract In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured... more Abstract In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured on 0.8% agar-solidified 1/2 MS basal medium containing B5 vitamins, 1.0 or 0.5 mg L–1 NAA, 5.0 or 10.0 mg L–1 BA and 3% sucrose. The frequency of adventitious bud formation was 30-45% in all combinations of NAA and BA. In histological observations, prominent mitotic figures were observed in several cells of the subepidermal palisade layers on the adaxial side of the expiants in contact with medium after 3 days of culture. Calli were formed within 6 days of culture. After 10 days of culture, numerous mature tracheary elements were produced at random in the proliferated regions, and cell divisions at the superficial region led to the formation of the meristematic structure. The shoot apex of the seedling produced numerous trichomes from superficial cells, but the adventitious bud formed on the cotyledon produced no trichomes. Initiation of the meristematic region in the expiant could be used as a target site for gene transfer experiments.

Journal of Plant Research, Feb 1, 2005

Acta horticulturae, Sep 1, 1988

Journal of Horticultural Science & Biotechnology, 2007

Summary Inter-section hybrids were successfully obtained from reciprocal crosses between tetraplo... more Summary Inter-section hybrids were successfully obtained from reciprocal crosses between tetraploid Primula denticulata (section Denticulata) and diploid P. rosea (section Oreophlomis) by applying ovary culture 4 weeks after pollination. Differences in ploidy levels were found in these hybrids by flow cytometric analysis and by counting chromosome numbers. When P. denticulata was used as maternal parent, all the hybrids were triploid, with two genomes of P. denticulata and one genome of P. rosea. In contrast, when P. rosea was used as maternal parent, two genome-type hybrids were obtained: one was a triploid hybrid, with the same genome combinations as those obtained when P. denticulata was used as maternal parent, and the other type was a tetraploid hybrid with two genomes of each species. These results suggest that unreduced (2x) gametes were formed only in the female side of P. rosea.

Plant Biotechnology, 1998

Zeitschrift für Pflanzenphysiologie, Nov 1, 1976

ABSTRACT Anthers of Nicotiana tabacum cv. Wisconsin 38 contammg pollen grains in various developm... more ABSTRACT Anthers of Nicotiana tabacum cv. Wisconsin 38 contammg pollen grains in various developmental stages were cultured for 55 days at 23°C to investigate the relationship between the formation of plantlets from pollen and browning of the anther. Browning took place in anthers containing binucleate pollen grains during the first 10 days after inoculation onto Nitsch's basal medium (1969), whereas it was continuously observed for as long as a month in anthers containing micros pores in the uninucleate stage and in those undergoing pollen mitosis. Plantlet formation in the first instance was observed during the first 3 to 5 weeks of culture and was never detected thereafter. There was no positive correlation between anther browning and plantlet formation. Plantlet induction was delayed for one week, but it was observed to be continuously effective until the cultures were 50 days old in the anthers containing uninucleate and mitotic microspores. The protraction of senescence in anthers appears to be of great importance for the induction of embryogenesis in microspores at this stage of development. There was a positive correlation between the duration of anther browning and the time required for plantlet production. That is, the percentage of plantlet formation in anthers having browned during the first 2 weeks was low, whereas the frequency of plantlet formation was high in long-lived anthers. These results suggest that there is a critical period for the induction of pollen embryogenesis in the early binucleate stage. Most microspores not yet having undergone pollen mitosis can attain to this stage with the nutritional or hormonal aid of living anther tissue; the embryogenesis can never proceed if anther browning has occurred before this stage has been attained. In a more advanced stage the duration of such a situation seems to be shorter.

Plant Cell Reports, Jun 1, 1992

Plant Cell Reports, Mar 1, 1995

Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containin... more Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containing 1 mg l-1 2,4-D and 2 g l-1 casein hydrolysate. Cell suspension cultures were readily established from these friable calli and protoplasts could be isolated from the cells with yields of 1–3×107/g f. wt.. By culturing in 0.1 % gellan gum-solidified B5 medium supplemented with 1 mg l-1 2,4-D and 0.1 M each of sucrose and mannitol at a density of 1×105/ml, the protoplasts divided within 6 days and formed macro-colonies after 2 months of culture. Shoot regeneration from protoplast-derived calli was obtained by sequential treatment of the calli with plant growth regulators: initially with 1 mg l-1 each of NAA and BA for 2 months followed by 0.01 mg l-1 NAA and 5 mg l-1 BA for 4 months. Regenerated plants were established after rooting of the shoots on half-strength MS medium, and successfully transferred to the greenhouse. The regenerated plants grew into flowering stage and showed the same phenotype as the parent plant.

Plant Science, May 1, 2001

Protoplasts were isolated from cell suspension cultures of Primula malacoides cv. 'Lovely To... more Protoplasts were isolated from cell suspension cultures of Primula malacoides cv. 'Lovely Tokyo' and P. obconica cv. 'Aalsmeer Giant White'. P. obconica protoplasts were embedded in 0.1% (w/v) gellan gum-solidified discs comprising MS medium supplemented with 3 mg/l ...

The Journal Of Horticultural Science And Biotechnology, 1974

SummaryThe excised bulb scales of amaryllis (Hippeastrum hybridum), cultured on Murashige and Sko... more SummaryThe excised bulb scales of amaryllis (Hippeastrum hybridum), cultured on Murashige and Skoog’s medium supplemented with combinations of NAA and kinetin, developed buds or roots or both together. The patterns of organogenesis were governed by the concentration of NAA in the medium. On the other hand, kinetin was not only less effective but was also toxic at high concentrations.

Plant Cell Tissue and Organ Culture, Oct 1, 1995

Plant Biotechnology Reports, Aug 20, 2010

Scientia Horticulturae, Mar 1, 1997

Scientia Horticulturae, Oct 1, 2011

Scientia Horticulturae, Sep 1, 1995

Scientia Horticulturae, Feb 1, 2009

... In “Egusi” relatives such as watermelon ([Dong and Jia, 1991] and [Yalcin-Mendi et al., 2003]... more ... In “Egusi” relatives such as watermelon ([Dong and Jia, 1991] and [Yalcin-Mendi et al., 2003]), Cucumis melo ([Akasaka-Kennedy et al., 2004] and [Rhimi et al., 2006]), cucumber ([Ahmad andAnis, 2005] and [Vasudevan et al., 2007]), muskmelon ([Fang and Grumet, 1990] and ...

Plant Biotechnology, 2010

Plant Biotechnology Reports, Dec 20, 2008

The effects of low temperature storage and cryopreservation on the survival and infectivity of Ro... more The effects of low temperature storage and cryopreservation on the survival and infectivity of Romanomermis culicivorax were studied in the laboratory. When pre-parasitic juveniles of R. culicivorax were stored at-2 to 2°C, more than 90% survived for 9 days and 50% were motile for 13 days. The infective ability of the pre-parasitic juveniles for mosquitoes remained high (85%) after 5 days of cold storage and the infectivity was reduced only moderately (50 to 78%) after storage for 6 to 10 days. Various cryoprotectants were investigated to develop a cryopreservation procedure for the infectious pre-parasitic juveniles of R. culicivorax. After suspension in 1, 2.5 or 5% dimethylsulphoxide (DMSO), and cryopreservation with a two-step cooling sequence prior to storage in liquid nitrogen, approximately half of the pre-parasitic juveniles of R. culicivorax, cryopreserved in 2.5 and 5% DMSO, regained motility when thawed quickly after storage for 7 and 125 days. However, revived pre-parasitic juveniles were unable to infect mosquito larvae. Pre-parasitic juveniles treated with ethanediol, hydroxyethyl starch, and polyvinylpyrrolidone as cryoprotectants did not survive the cryopreservation procedure. Similar results were obtained with the pre-parasitic juveniles (PPJ) of Romanomermis yunanensis.

Plant Cell Reports, Sep 4, 1997

Plant Cell Reports, Feb 1, 1996

Plant Production Science, 2002

Abstract In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured... more Abstract In Crotalaria juncea L., adventitious buds were formed in cotyledonary expiants cultured on 0.8% agar-solidified 1/2 MS basal medium containing B5 vitamins, 1.0 or 0.5 mg L–1 NAA, 5.0 or 10.0 mg L–1 BA and 3% sucrose. The frequency of adventitious bud formation was 30-45% in all combinations of NAA and BA. In histological observations, prominent mitotic figures were observed in several cells of the subepidermal palisade layers on the adaxial side of the expiants in contact with medium after 3 days of culture. Calli were formed within 6 days of culture. After 10 days of culture, numerous mature tracheary elements were produced at random in the proliferated regions, and cell divisions at the superficial region led to the formation of the meristematic structure. The shoot apex of the seedling produced numerous trichomes from superficial cells, but the adventitious bud formed on the cotyledon produced no trichomes. Initiation of the meristematic region in the expiant could be used as a target site for gene transfer experiments.

Journal of Plant Research, Feb 1, 2005

Acta horticulturae, Sep 1, 1988

Journal of Horticultural Science & Biotechnology, 2007

Summary Inter-section hybrids were successfully obtained from reciprocal crosses between tetraplo... more Summary Inter-section hybrids were successfully obtained from reciprocal crosses between tetraploid Primula denticulata (section Denticulata) and diploid P. rosea (section Oreophlomis) by applying ovary culture 4 weeks after pollination. Differences in ploidy levels were found in these hybrids by flow cytometric analysis and by counting chromosome numbers. When P. denticulata was used as maternal parent, all the hybrids were triploid, with two genomes of P. denticulata and one genome of P. rosea. In contrast, when P. rosea was used as maternal parent, two genome-type hybrids were obtained: one was a triploid hybrid, with the same genome combinations as those obtained when P. denticulata was used as maternal parent, and the other type was a tetraploid hybrid with two genomes of each species. These results suggest that unreduced (2x) gametes were formed only in the female side of P. rosea.

Plant Biotechnology, 1998

Zeitschrift für Pflanzenphysiologie, Nov 1, 1976

ABSTRACT Anthers of Nicotiana tabacum cv. Wisconsin 38 contammg pollen grains in various developm... more ABSTRACT Anthers of Nicotiana tabacum cv. Wisconsin 38 contammg pollen grains in various developmental stages were cultured for 55 days at 23°C to investigate the relationship between the formation of plantlets from pollen and browning of the anther. Browning took place in anthers containing binucleate pollen grains during the first 10 days after inoculation onto Nitsch's basal medium (1969), whereas it was continuously observed for as long as a month in anthers containing micros pores in the uninucleate stage and in those undergoing pollen mitosis. Plantlet formation in the first instance was observed during the first 3 to 5 weeks of culture and was never detected thereafter. There was no positive correlation between anther browning and plantlet formation. Plantlet induction was delayed for one week, but it was observed to be continuously effective until the cultures were 50 days old in the anthers containing uninucleate and mitotic microspores. The protraction of senescence in anthers appears to be of great importance for the induction of embryogenesis in microspores at this stage of development. There was a positive correlation between the duration of anther browning and the time required for plantlet production. That is, the percentage of plantlet formation in anthers having browned during the first 2 weeks was low, whereas the frequency of plantlet formation was high in long-lived anthers. These results suggest that there is a critical period for the induction of pollen embryogenesis in the early binucleate stage. Most microspores not yet having undergone pollen mitosis can attain to this stage with the nutritional or hormonal aid of living anther tissue; the embryogenesis can never proceed if anther browning has occurred before this stage has been attained. In a more advanced stage the duration of such a situation seems to be shorter.

Plant Cell Reports, Jun 1, 1992

Plant Cell Reports, Mar 1, 1995

Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containin... more Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containing 1 mg l-1 2,4-D and 2 g l-1 casein hydrolysate. Cell suspension cultures were readily established from these friable calli and protoplasts could be isolated from the cells with yields of 1–3×107/g f. wt.. By culturing in 0.1 % gellan gum-solidified B5 medium supplemented with 1 mg l-1 2,4-D and 0.1 M each of sucrose and mannitol at a density of 1×105/ml, the protoplasts divided within 6 days and formed macro-colonies after 2 months of culture. Shoot regeneration from protoplast-derived calli was obtained by sequential treatment of the calli with plant growth regulators: initially with 1 mg l-1 each of NAA and BA for 2 months followed by 0.01 mg l-1 NAA and 5 mg l-1 BA for 4 months. Regenerated plants were established after rooting of the shoots on half-strength MS medium, and successfully transferred to the greenhouse. The regenerated plants grew into flowering stage and showed the same phenotype as the parent plant.

Plant Science, May 1, 2001

Protoplasts were isolated from cell suspension cultures of Primula malacoides cv. 'Lovely To... more Protoplasts were isolated from cell suspension cultures of Primula malacoides cv. 'Lovely Tokyo' and P. obconica cv. 'Aalsmeer Giant White'. P. obconica protoplasts were embedded in 0.1% (w/v) gellan gum-solidified discs comprising MS medium supplemented with 3 mg/l ...

The Journal Of Horticultural Science And Biotechnology, 1974

SummaryThe excised bulb scales of amaryllis (Hippeastrum hybridum), cultured on Murashige and Sko... more SummaryThe excised bulb scales of amaryllis (Hippeastrum hybridum), cultured on Murashige and Skoog’s medium supplemented with combinations of NAA and kinetin, developed buds or roots or both together. The patterns of organogenesis were governed by the concentration of NAA in the medium. On the other hand, kinetin was not only less effective but was also toxic at high concentrations.

Plant Cell Tissue and Organ Culture, Oct 1, 1995

Plant Biotechnology Reports, Aug 20, 2010

Scientia Horticulturae, Mar 1, 1997

Scientia Horticulturae, Oct 1, 2011

Scientia Horticulturae, Sep 1, 1995

Scientia Horticulturae, Feb 1, 2009

... In “Egusi” relatives such as watermelon ([Dong and Jia, 1991] and [Yalcin-Mendi et al., 2003]... more ... In “Egusi” relatives such as watermelon ([Dong and Jia, 1991] and [Yalcin-Mendi et al., 2003]), Cucumis melo ([Akasaka-Kennedy et al., 2004] and [Rhimi et al., 2006]), cucumber ([Ahmad andAnis, 2005] and [Vasudevan et al., 2007]), muskmelon ([Fang and Grumet, 1990] and ...

Plant Biotechnology, 2010

Plant Biotechnology Reports, Dec 20, 2008

The effects of low temperature storage and cryopreservation on the survival and infectivity of Ro... more The effects of low temperature storage and cryopreservation on the survival and infectivity of Romanomermis culicivorax were studied in the laboratory. When pre-parasitic juveniles of R. culicivorax were stored at-2 to 2°C, more than 90% survived for 9 days and 50% were motile for 13 days. The infective ability of the pre-parasitic juveniles for mosquitoes remained high (85%) after 5 days of cold storage and the infectivity was reduced only moderately (50 to 78%) after storage for 6 to 10 days. Various cryoprotectants were investigated to develop a cryopreservation procedure for the infectious pre-parasitic juveniles of R. culicivorax. After suspension in 1, 2.5 or 5% dimethylsulphoxide (DMSO), and cryopreservation with a two-step cooling sequence prior to storage in liquid nitrogen, approximately half of the pre-parasitic juveniles of R. culicivorax, cryopreserved in 2.5 and 5% DMSO, regained motility when thawed quickly after storage for 7 and 125 days. However, revived pre-parasitic juveniles were unable to infect mosquito larvae. Pre-parasitic juveniles treated with ethanediol, hydroxyethyl starch, and polyvinylpyrrolidone as cryoprotectants did not survive the cryopreservation procedure. Similar results were obtained with the pre-parasitic juveniles (PPJ) of Romanomermis yunanensis.

Plant Cell Reports, Sep 4, 1997

Plant Cell Reports, Feb 1, 1996