Matthew May - Academia.edu (original) (raw)

Papers by Matthew May

Idaho’s College and Career Advising and Mentoring Program is a five-year intervention intended to... more Idaho’s College and Career Advising and Mentoring Program is a five-year intervention intended to enable Idaho’s Local Educational Agencies’ (both school districts and charter schools) efforts to support Idaho students’ preparation for college and career readiness. Such efforts include opportunities for students to identify strengths, areas for improvement and areas of interest in regard to career and postsecondary education goals. In 2018, the Idaho Legislature requested an independent evaluation of the Program. This report, by the Idaho Policy Institute, serves as that evaluation. Student-level data from the Idaho Department of Education, school-level data from the National Center for Education Statistics, and Local Educational Agency plans from the Idaho State Board of Education were used to examine program participation and outcomes. The evaluation considers the Program’s design, use of funds, effectiveness and several other relevant metrics. Analysis reveals a number of issues ...

There is growing concern about the financial burden of balance billing, or surprise billing, on i... more There is growing concern about the financial burden of balance billing, or surprise billing, on individuals with private health insurance plans. Idaho has limited provisions concerning balance billing, but none that protect patients who are unaware they are receiving services outside their insurance carrier’s network. Idaho residents receiving emergency care or major surgeries often do not choose their treating physicians, putting them at risk of being charged staggering amounts for out-of-network services. Research shows that balance billing disparities exist between geographic areas and service type which may point to private health insurance network inadequacies. Half of states have full or partial protections for patients experiencing balance billing situations and legislation is currently being considered by Congress. The purpose of this report is to examine balance billing in Idaho. This study analyzes claims data provided by Idaho’s major insurance carriers and a survey of ph...

Frontiers in medicine, 2018

The main aim of health technology assessment (HTA) is to inform decision making by health care po... more The main aim of health technology assessment (HTA) is to inform decision making by health care policy makers. It is a systematic process that evaluates the use of health technologies and generally involves a critical review of international evidence related to clinical effectiveness of the health technology vs. the best standard of care. It can also include an evaluation of cost effectiveness, and social and ethical impacts in the local health care system. The HTA process advises whether or not a health technology should be used, and if so, how it is best used and which patients are most likely to benefit from it. The importance of patient involvement in HTA is becoming widely recognized, for scientific and democratic reasons. The extent of patient involvement in HTA varies considerably across Europe. Commonly HTA is still focused on quantitative evidence to determine clinical and/or cost effectiveness, but the interest in understanding patients' experiences and preferences is i...

Frontiers in medicine, 2018

The importance and merits of greater patient involvement in medicines research and development (R... more The importance and merits of greater patient involvement in medicines research and development (R&D) are commonly acknowledged and is thought to offer benefits for all involved parties. It improves discovery, development, and evaluation of new effective medicines, based, among others, on the collaborative identification and understanding of unmet needs, research priorities, optimization of clinical study design, as well as incorporating patient views in regulatory activities. It fosters increased transparency, trust and mutual respect between patients and other stakeholders and applies to all stages of medicines R&D, inclusive of regulation and licensing of medicines and appraisal by health technology assessment (HTA) bodies. In order to be effective and beneficial for all stakeholders, patient engagement as an integral part of medicines R&D needs clear and mutually agreed rules. Existing codes of practice for patient involvement do not comprehensively cover the full scope of patien...

Teaching Sociology, 2016

This article describes a simulation activity designed to teach students about the wage gap. The w... more This article describes a simulation activity designed to teach students about the wage gap. The wage gap is an important topic in many sociology classrooms, but it can be difficult to convey the accumulated disadvantage experienced by women and racial/ethnic minorities to students using in-class discussions, lectures, or assigned readings alone. This is particularly true on college campuses that may draw their students from more affluent areas. Classroom simulations, however, provide an opportunity for students with all types of backgrounds to engage their sociological imaginations. In our simulation—Intersectionopoly—we use a modified version of Monopoly based on the wage gap and racial/ethnic minorities’ experiences of everyday life to illustrate how members of different racial and gender groups experience disparities in earnings. Unlike other versions of stratified Monopoly, this simulation more closely mirrors the subtle nature of discrimination in the contemporary United States.

BMJ open, Jan 8, 2015

To explore public knowledge of, and interest in, learning more about medicines R&D in six Europea... more To explore public knowledge of, and interest in, learning more about medicines R&D in six European countries. Online survey of 6931 members of the public across Europe. The survey formed part of a public omnibus survey. A quota sampling approach was used with quotas set according to national census data on age, gender and government region. The survey explored the public's knowledge and awareness of medicines R&D, their interest in learning more and the perceived influences on this. The survey was completed by 6931 members of the public, over 75% of whom reported having no or less than good knowledge of medicines R&D. Males were more likely than females to report good knowledge (17% vs 15%), and knowledge appeared to decrease with age. Those who were currently or had previously been involved in medical research were almost five times more likely to report good knowledge of medicines R&D overall (43% vs 13%). Participants reported good knowledge of medicines safety and clinical t...

Proceedings of the SIGCHI Conference on Human Factors in Computing Systems, 2010

Stroke is the leading cause of long term disability among adults in industrialized nations. The p... more Stroke is the leading cause of long term disability among adults in industrialized nations. The partial paralysis that stroke patients often experience can make independent living difficult or impossible. Research suggests that many of these patients could recover by performing hundreds of daily repetitions of motions with their affected limbs. Yet, only 31% of patients perform the exercises recommended by their therapists. Home-based stroke rehabilitation games may help motivate stroke patients to perform the necessary exercises to recover. In this paper, we describe a formative study in which we designed and user tested stroke rehabilitation games with both stroke patients and therapists. We describe the lessons we learned about what makes games useful from a therapeutic point of view.

Quarterly Journal of Speech, 2011

... 1967). For the IWW, a hobo was a term of endearment for a migratory worker, either working or... more ... 1967). For the IWW, a hobo was a term of endearment for a migratory worker, either working or looking for work. The term bum usually referred to someone who was sedentary (non-migratory) and permanently unemployed. ...

Molecular Microbiology, 2002

In Gram-negative bacteria, including Rhodobacter capsulatus, the membrane protein CycH acts as a ... more In Gram-negative bacteria, including Rhodobacter capsulatus, the membrane protein CycH acts as a putative apocytochrome chaperone during the biogenesis of c-type cytochromes. CycH-null mutants are unable to produce various c-type cytochromes and sustain photosynthetic (Ps) growth that requires the cytochromes c1 and c2 or cy. However, Ps+ revertants are readily obtained only on minimal, but not on enriched, medium. To obtain further information about the biogenesis of c-type cytochromes, these suppressor mutants were studied. Complementation of a CycH-null mutant for Ps+ growth by a genomic library constructed using DNA from a Ps+ suppressor yielded a plasmid carrying the ccl1-2 operon, the products of which, Ccl1 and Ccl2, are also involved in the biogenesis of c-type cytochromes. DNA sequence analysis revealed that the complementing activity resulted from a single point mutation, G488A, located upstream of the coding region of ccl1-2. This mutation changed the -35 region of the ccl1-2 promoter from TTGGCC to TTGACC, improving its similarity to the consensus sequence of Escherichia colisigma 70-dependent promoters. That the G488A mutation indeed enhanced transcription of ccl1-2 was demonstrated by the use of reporter gene fusions. An appropriate ccl1-2::lacZ transcriptional-translational fusion carrying the G488A mutation produced in R. capsulatus over 30-fold higher beta-galactosidase activity than a wild-type construct. Immunoblot analyses confirmed that Ccl1 and Ccl2 were overproduced in the Ps+ suppressors. Deletion of either ccl1 or ccl2, from the ccl1-2 cluster carrying the G488A mutation abolished the complementing ability, indicating that overexpression of both ccl1 and ccl2 was required to confer the Ps+ phenotype on a CycH-null mutant. These findings therefore demonstrate that, during R. capsulatus growth on minimal medium, the requirement for CycH in c-type cytochrome biogenesis could be bypassed by overexpressing the ccl1-2 operon.

Communication and Critical/Cultural Studies, 2009

... Althusser is referencing Spinoza's epistemological claim that truth is the standard of it... more ... Althusser is referencing Spinoza's epistemological claim that truth is the standard of itself and of the false. I wish to thank Cesare Casarino for this insight. ... Althusser is referencing Spinoza's epistemological claim that truth is the standard of itself and of the false. ...

Analytical Biochemistry, 1982

ABSTRACT A gas chromatography-mass spectrometric method is described for the precise chemical ide... more ABSTRACT A gas chromatography-mass spectrometric method is described for the precise chemical identification of the sugar residues of daunorubicin and the N-alkylated anthracycline analogs. Parent drug or purified biliary metabolites from rabbits were hydrolyzed with 0.2 n HCl for 15 to 30 min at 100°C. The generated aglycones were removed by chloroform extraction, and the remaining aqueous extract was evaporated under nitrogen. The residue was dissolved in trimethylsilylimidazole/pyridine. The resulting silylated daunosamine moiety was chromatographed on a 3% OV-17 column coupled via a jet separator to the source of a mass spectrometer. The natural α-anomer, which was assigned to the major peak, eluted first. Electron ionization analysis of the silylated sugars displayed extensive fragmentation. Isobutane chemical ionization provided spectra with intense protonated molecular ions [MH]+ and limited fragmentation. In all cases the later eluting β-anomer gave identical although more intense fragments than the α-anomer. Common fragment ions seen were [MH]+, [MH-90]+, [MH-116]+, and [MH-130]+. Daunosamine has been detected from as little as 5.7 nmol daunorubicin. N-Benzyldaunorubicinol was identified as the major biliary metabolite in rabbits following dosing with 3.0 mg/kg N,N-dibenzyldaunorubicin. This method has been applied to the analysis of glucosamine and may find utility in the analysis of many other amino sugars.

From 1909 to 1916 thousands of hobos joined the Industrial Workers of the World and participated ... more From 1909 to 1916 thousands of hobos joined the Industrial Workers of the World and participated in major fights for free speech in several dozen cities in the American west. During this period, the union organized over two dozen confrontations with municipal authorities to challenge repressive speaking laws which they considered to be de facto injunctions against public organizing. The myriad tactics involved in the free speech fights transformed over time to meet the new challenges presented by various forces of repression; but the fights were always anchored in the practice of violating repressive ordinances by speaking on a soapbox. Many of the participants were arrested and barricaded in the bastilles of the American west. Some were beaten, publicly humiliated, killed, or eventually deported. This dissertation explores how the performance of soapbox oratory composed waged and unwaged workers as a class. The study is organized chronologically by date according to the major free speech fights in Spokane, Fresno, San Diego, and Everett. I argue that the hobo orators of the free speech fights demonstrate the significance of the oratorical as a revolutionary practice of class composition. In this regard, the dissertation seeks to reveal lessons about the possibilities of revolutionary unionism today. iii Table of Contents Introduction: The Free Speech Fights of the Industrial Workers of the World……....1

In 2017, the Idaho State Department of Education entered into a contract with Istation to improve... more In 2017, the Idaho State Department of Education entered into a contract with Istation to improve the Idaho Reading Indicator (IRI), Idaho’s statewide testing platform for kindergarten through third grade (K-3). The implementation of the new Istation IRI assessment is set to be phased in over two years. To begin this process, in academic year 2017-2018, Istation engaged 58 schools across the State of Idaho in a pilot program for the new IRI assessment. In response to a request by members of the Joint Finance-Appropriations Committee (JFAC) of the Idaho State Legislature, the Idaho Policy Institute (IPI) began conducting an independent evaluation of the implementation of this pilot study. This phase of IPI’s study was structured exclusively around the experiences of K-3 teachers with administering and utilizing the output of the Istation IRI assessment

Trees, Forests and People, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Cancer Chemotherapy and Pharmacology, 1982

A phase II study of intermittent high-dose 6-thioguanine (6-TG) was undertaken in 19 patients wit... more A phase II study of intermittent high-dose 6-thioguanine (6-TG) was undertaken in 19 patients with metastatic colorectal carcinoma. Fourteen patients had received prior myelosuppressive therapy. 6-TG was administered as a single dose by 1V bolus over 15-30 rain, with retreatment every 3 weeks. The starting dose was 700 mg/m 2 in ten patients, 900 mg/m 2 in one patient, 1,000 mg/m 2 in four patients, and 1,200 mg/m 2 in two patients. Two patients received reduced doses (350 mg/m 2) because of liver dysfunction. There was no regression of measurable disease after treatment with 6-TG in this study. Eight patients achieved stabilization of previously progressive disease for periods of 10-32 weeks. Toxicities were nausea and vomiting (19 patients), mucositis (3 patients), reversible renal dysfunction with creatinine > 2 mg/dl (4 patients), nasal congestion (3 patients), diarrhea (1 patient), and skin blistering at the infusion site (1 patient). Seven patients had white blood count nadirs below 3,000/~tl (the lowest nadir was 900/~tl). Only one patient had a platelet count nadir below lO0,O00/~tl. There were no infections or hemorrhage. 6-TG, as administered in this study, has no antitumor activity against colorectal carcinoma. Concentrations of 6-TG and metabolites were assessed in the plasma of six patients by a reversed-phase HPLC system. 6-TG and metabolites were extracted from human plasma at 50%-100% efficiency by cold 2 N perchloric acid (1 : 1). Neutralized extracts were chromatographed on a ~t-Bondapak Cls column by two separate isocratic conditions. 6-TG, 6-thiouric acid, 6-thioguanosine, and 6-thioxanthine were analyzed with 0.01 M Na acetate, pH3.5/10% methanol as the mobile phase and were detected at 340 nm. 6-Methyl TG and three Reprint requests should be addressed to M. J. Egorin * This paper was presented in part at the 72rid Annual Meeting of the American Association for Cancer Research, Washington, DC, 1981 unknown metabolites were eluted with Na acetate~25% methanol and were detected at 310 nm. External standard calibration was used for quantitation. The 6-TG detection limit was 0.8 nmol/ml. In six patients who received 1-1.2 g 6-TG/m 2 IV, 6-TG achieved peak plasma concentrations of 61-118 nmol/ml (95.6 +_ 23.0, mean + SD). Plasma 6-TG concentrations decayed bi-exponentially, with initial tl/2 of 3 h and terminal q/2 of 5.9 h. 6-Thiouric acid, 6-methyl TG, 6-thioguanosine, 6-thioxanthine, and three major unidentified metabolites were also observed in plasma. The three unknowns were extracted with ethyl acetate from alkalinized pooled plasma extracts and were purified by HPLC.

The Professional Animal Scientist, 2015

The molecule 2-methyl-2-butenal (2M2B) is a maternal pheromone in rabbits and an interomone in do... more The molecule 2-methyl-2-butenal (2M2B) is a maternal pheromone in rabbits and an interomone in dogs eliciting behavioral and heart-rate changes. The molecule 2M2B is manufactured in dog collars (Nurturecalm 24/

The Professional Animal Scientist, 2010

Effects of 3 levels of distillers grains (DG) and 3 ratios of steam-flaked corn (SFC) to ground c... more Effects of 3 levels of distillers grains (DG) and 3 ratios of steam-flaked corn (SFC) to ground corn (GC) were used to evaluate IVDMD, in vitro gas production kinetics, and in vitro H 2 S production using ruminal fluid from 2 Jersey crossbred steers. Substrates included either corn (CDG) or sorghum distillers grains (SDG). Within each type of DG, treatments were 0, 15, or 30% DG (DM basis) and ratios of SFC:GC of 50:50, 75:25, or 100:0. With CDG, IVDMD tended (P = 0.06) to increase linearly as SFC increased in substrates, and increasing CDG increased H 2 S production (linear, P < 0.01). Fractional rate of gas production tended to decrease quadratically (P = 0.07) with decreased SFC in substrates. Molar proportion of propionate (P = 0.05) and acetate:propionate ratio (A:P; P = 0.03) responded quadratically to CDG level, with the greatest values at 15% DG. With SDG, IVDMD decreased (P = 0.02) as the percentage of SFC decreased and tended (P = 0.09) to increase as SDG level increased. Similar to CDG, H 2 S production increased linearly (P = 0.02) as SDG increased from 0 to 30% of the DM. Fractional rate of gas production was greatest (quadratic, P = 0.04) for 75:25 ratios of SFC to GC and was not affected by SDG level (P > 0.23). Total VFA concentration decreased (P = 0.03) as proportion of SFC decreased, and increasing SDG tended to decrease propionate (P = 0.09) and increase A:P (P = 0.06). Overall, increasing concentration of CDG or SDG increased in vitro H 2 S production. Effects of DG concentration were largely independent of the percentage of SFC in the substrate for IVDMD and gas production kinetics, but adding DG tended to increase A:P proportions in vitro.

The Professional Animal Scientist, 2011

Effects of substrates containing different proportions of distillers solubles (DS) to distillers ... more Effects of substrates containing different proportions of distillers solubles (DS) to distillers grain (DG) on IVDMD, H 2 S production, gas production kinetics, VFA proportions, and culture fluid osmolality were evaluated in a randomized block design with a 2 × 3 + 1 factorial arrangement of treatments. Treatment substrates consisted of 15 or 30% DG with DS added within each DG concentration to yield proportions of DG:DS of 100:0, 75:25, and 50:50; the steam-flaked corn-based control treatment contained no DG. Two ruminally cannulated Jersey crossbred steers (BW = 590 kg) fed a 60% concentrate, steam-flaked corn-based diet were used as ruminal fluid donors. Duplicate cultures were incubated for 24 h to measure IVDMD and 48 h for gas production kinetics, with incubations replicated on separate days. No differences were observed among treatments for IVDMD (P > 0.15), but H 2 S production was increased (P < 0.01) by 39 and 73% for 15 and 30% DG, respectively, relative to the control. Moreover, H 2 S production increased linearly as the proportion of DS increased (P < 0.01). Osmolality was greater for the control (P < 0.001) than for other treatments, decreased (P < 0.001) with increasing DG concentration, increased linearly (P < 0.001) with increased DS in substrates, and increased over time (P < 0.001). Fractional rate of gas production (h −1) was less (P = 0.01) for 30 versus 15% DG, and lag time of gas production decreased as DS increased in substrates (linear, P = 0.02). Molar proportion of acetate was least (P = 0.02), propionate was greatest (P < 0.01), and acetate:propionate ratio was least (P = 0.02) for the control versus other treatments. In general, including DG in substrates and increasing proportions of DS increased H 2 S production, reflecting increased S concentrations. For both 15 and 30% DG, substrates with greater proportions of DS had increased total gas production and culture fluid osmolality.

Kansas Agricultural Experiment Station Research Reports, 2008

Kansas Agricultural Experiment Station Research Reports, 2007

Idaho’s College and Career Advising and Mentoring Program is a five-year intervention intended to... more Idaho’s College and Career Advising and Mentoring Program is a five-year intervention intended to enable Idaho’s Local Educational Agencies’ (both school districts and charter schools) efforts to support Idaho students’ preparation for college and career readiness. Such efforts include opportunities for students to identify strengths, areas for improvement and areas of interest in regard to career and postsecondary education goals. In 2018, the Idaho Legislature requested an independent evaluation of the Program. This report, by the Idaho Policy Institute, serves as that evaluation. Student-level data from the Idaho Department of Education, school-level data from the National Center for Education Statistics, and Local Educational Agency plans from the Idaho State Board of Education were used to examine program participation and outcomes. The evaluation considers the Program’s design, use of funds, effectiveness and several other relevant metrics. Analysis reveals a number of issues ...

There is growing concern about the financial burden of balance billing, or surprise billing, on i... more There is growing concern about the financial burden of balance billing, or surprise billing, on individuals with private health insurance plans. Idaho has limited provisions concerning balance billing, but none that protect patients who are unaware they are receiving services outside their insurance carrier’s network. Idaho residents receiving emergency care or major surgeries often do not choose their treating physicians, putting them at risk of being charged staggering amounts for out-of-network services. Research shows that balance billing disparities exist between geographic areas and service type which may point to private health insurance network inadequacies. Half of states have full or partial protections for patients experiencing balance billing situations and legislation is currently being considered by Congress. The purpose of this report is to examine balance billing in Idaho. This study analyzes claims data provided by Idaho’s major insurance carriers and a survey of ph...

Frontiers in medicine, 2018

The main aim of health technology assessment (HTA) is to inform decision making by health care po... more The main aim of health technology assessment (HTA) is to inform decision making by health care policy makers. It is a systematic process that evaluates the use of health technologies and generally involves a critical review of international evidence related to clinical effectiveness of the health technology vs. the best standard of care. It can also include an evaluation of cost effectiveness, and social and ethical impacts in the local health care system. The HTA process advises whether or not a health technology should be used, and if so, how it is best used and which patients are most likely to benefit from it. The importance of patient involvement in HTA is becoming widely recognized, for scientific and democratic reasons. The extent of patient involvement in HTA varies considerably across Europe. Commonly HTA is still focused on quantitative evidence to determine clinical and/or cost effectiveness, but the interest in understanding patients' experiences and preferences is i...

Frontiers in medicine, 2018

The importance and merits of greater patient involvement in medicines research and development (R... more The importance and merits of greater patient involvement in medicines research and development (R&D) are commonly acknowledged and is thought to offer benefits for all involved parties. It improves discovery, development, and evaluation of new effective medicines, based, among others, on the collaborative identification and understanding of unmet needs, research priorities, optimization of clinical study design, as well as incorporating patient views in regulatory activities. It fosters increased transparency, trust and mutual respect between patients and other stakeholders and applies to all stages of medicines R&D, inclusive of regulation and licensing of medicines and appraisal by health technology assessment (HTA) bodies. In order to be effective and beneficial for all stakeholders, patient engagement as an integral part of medicines R&D needs clear and mutually agreed rules. Existing codes of practice for patient involvement do not comprehensively cover the full scope of patien...

Teaching Sociology, 2016

This article describes a simulation activity designed to teach students about the wage gap. The w... more This article describes a simulation activity designed to teach students about the wage gap. The wage gap is an important topic in many sociology classrooms, but it can be difficult to convey the accumulated disadvantage experienced by women and racial/ethnic minorities to students using in-class discussions, lectures, or assigned readings alone. This is particularly true on college campuses that may draw their students from more affluent areas. Classroom simulations, however, provide an opportunity for students with all types of backgrounds to engage their sociological imaginations. In our simulation—Intersectionopoly—we use a modified version of Monopoly based on the wage gap and racial/ethnic minorities’ experiences of everyday life to illustrate how members of different racial and gender groups experience disparities in earnings. Unlike other versions of stratified Monopoly, this simulation more closely mirrors the subtle nature of discrimination in the contemporary United States.

BMJ open, Jan 8, 2015

To explore public knowledge of, and interest in, learning more about medicines R&D in six Europea... more To explore public knowledge of, and interest in, learning more about medicines R&D in six European countries. Online survey of 6931 members of the public across Europe. The survey formed part of a public omnibus survey. A quota sampling approach was used with quotas set according to national census data on age, gender and government region. The survey explored the public's knowledge and awareness of medicines R&D, their interest in learning more and the perceived influences on this. The survey was completed by 6931 members of the public, over 75% of whom reported having no or less than good knowledge of medicines R&D. Males were more likely than females to report good knowledge (17% vs 15%), and knowledge appeared to decrease with age. Those who were currently or had previously been involved in medical research were almost five times more likely to report good knowledge of medicines R&D overall (43% vs 13%). Participants reported good knowledge of medicines safety and clinical t...

Proceedings of the SIGCHI Conference on Human Factors in Computing Systems, 2010

Stroke is the leading cause of long term disability among adults in industrialized nations. The p... more Stroke is the leading cause of long term disability among adults in industrialized nations. The partial paralysis that stroke patients often experience can make independent living difficult or impossible. Research suggests that many of these patients could recover by performing hundreds of daily repetitions of motions with their affected limbs. Yet, only 31% of patients perform the exercises recommended by their therapists. Home-based stroke rehabilitation games may help motivate stroke patients to perform the necessary exercises to recover. In this paper, we describe a formative study in which we designed and user tested stroke rehabilitation games with both stroke patients and therapists. We describe the lessons we learned about what makes games useful from a therapeutic point of view.

Quarterly Journal of Speech, 2011

... 1967). For the IWW, a hobo was a term of endearment for a migratory worker, either working or... more ... 1967). For the IWW, a hobo was a term of endearment for a migratory worker, either working or looking for work. The term bum usually referred to someone who was sedentary (non-migratory) and permanently unemployed. ...

Molecular Microbiology, 2002

In Gram-negative bacteria, including Rhodobacter capsulatus, the membrane protein CycH acts as a ... more In Gram-negative bacteria, including Rhodobacter capsulatus, the membrane protein CycH acts as a putative apocytochrome chaperone during the biogenesis of c-type cytochromes. CycH-null mutants are unable to produce various c-type cytochromes and sustain photosynthetic (Ps) growth that requires the cytochromes c1 and c2 or cy. However, Ps+ revertants are readily obtained only on minimal, but not on enriched, medium. To obtain further information about the biogenesis of c-type cytochromes, these suppressor mutants were studied. Complementation of a CycH-null mutant for Ps+ growth by a genomic library constructed using DNA from a Ps+ suppressor yielded a plasmid carrying the ccl1-2 operon, the products of which, Ccl1 and Ccl2, are also involved in the biogenesis of c-type cytochromes. DNA sequence analysis revealed that the complementing activity resulted from a single point mutation, G488A, located upstream of the coding region of ccl1-2. This mutation changed the -35 region of the ccl1-2 promoter from TTGGCC to TTGACC, improving its similarity to the consensus sequence of Escherichia colisigma 70-dependent promoters. That the G488A mutation indeed enhanced transcription of ccl1-2 was demonstrated by the use of reporter gene fusions. An appropriate ccl1-2::lacZ transcriptional-translational fusion carrying the G488A mutation produced in R. capsulatus over 30-fold higher beta-galactosidase activity than a wild-type construct. Immunoblot analyses confirmed that Ccl1 and Ccl2 were overproduced in the Ps+ suppressors. Deletion of either ccl1 or ccl2, from the ccl1-2 cluster carrying the G488A mutation abolished the complementing ability, indicating that overexpression of both ccl1 and ccl2 was required to confer the Ps+ phenotype on a CycH-null mutant. These findings therefore demonstrate that, during R. capsulatus growth on minimal medium, the requirement for CycH in c-type cytochrome biogenesis could be bypassed by overexpressing the ccl1-2 operon.

Communication and Critical/Cultural Studies, 2009

... Althusser is referencing Spinoza's epistemological claim that truth is the standard of it... more ... Althusser is referencing Spinoza's epistemological claim that truth is the standard of itself and of the false. I wish to thank Cesare Casarino for this insight. ... Althusser is referencing Spinoza's epistemological claim that truth is the standard of itself and of the false. ...

Analytical Biochemistry, 1982

ABSTRACT A gas chromatography-mass spectrometric method is described for the precise chemical ide... more ABSTRACT A gas chromatography-mass spectrometric method is described for the precise chemical identification of the sugar residues of daunorubicin and the N-alkylated anthracycline analogs. Parent drug or purified biliary metabolites from rabbits were hydrolyzed with 0.2 n HCl for 15 to 30 min at 100°C. The generated aglycones were removed by chloroform extraction, and the remaining aqueous extract was evaporated under nitrogen. The residue was dissolved in trimethylsilylimidazole/pyridine. The resulting silylated daunosamine moiety was chromatographed on a 3% OV-17 column coupled via a jet separator to the source of a mass spectrometer. The natural α-anomer, which was assigned to the major peak, eluted first. Electron ionization analysis of the silylated sugars displayed extensive fragmentation. Isobutane chemical ionization provided spectra with intense protonated molecular ions [MH]+ and limited fragmentation. In all cases the later eluting β-anomer gave identical although more intense fragments than the α-anomer. Common fragment ions seen were [MH]+, [MH-90]+, [MH-116]+, and [MH-130]+. Daunosamine has been detected from as little as 5.7 nmol daunorubicin. N-Benzyldaunorubicinol was identified as the major biliary metabolite in rabbits following dosing with 3.0 mg/kg N,N-dibenzyldaunorubicin. This method has been applied to the analysis of glucosamine and may find utility in the analysis of many other amino sugars.

From 1909 to 1916 thousands of hobos joined the Industrial Workers of the World and participated ... more From 1909 to 1916 thousands of hobos joined the Industrial Workers of the World and participated in major fights for free speech in several dozen cities in the American west. During this period, the union organized over two dozen confrontations with municipal authorities to challenge repressive speaking laws which they considered to be de facto injunctions against public organizing. The myriad tactics involved in the free speech fights transformed over time to meet the new challenges presented by various forces of repression; but the fights were always anchored in the practice of violating repressive ordinances by speaking on a soapbox. Many of the participants were arrested and barricaded in the bastilles of the American west. Some were beaten, publicly humiliated, killed, or eventually deported. This dissertation explores how the performance of soapbox oratory composed waged and unwaged workers as a class. The study is organized chronologically by date according to the major free speech fights in Spokane, Fresno, San Diego, and Everett. I argue that the hobo orators of the free speech fights demonstrate the significance of the oratorical as a revolutionary practice of class composition. In this regard, the dissertation seeks to reveal lessons about the possibilities of revolutionary unionism today. iii Table of Contents Introduction: The Free Speech Fights of the Industrial Workers of the World……....1

In 2017, the Idaho State Department of Education entered into a contract with Istation to improve... more In 2017, the Idaho State Department of Education entered into a contract with Istation to improve the Idaho Reading Indicator (IRI), Idaho’s statewide testing platform for kindergarten through third grade (K-3). The implementation of the new Istation IRI assessment is set to be phased in over two years. To begin this process, in academic year 2017-2018, Istation engaged 58 schools across the State of Idaho in a pilot program for the new IRI assessment. In response to a request by members of the Joint Finance-Appropriations Committee (JFAC) of the Idaho State Legislature, the Idaho Policy Institute (IPI) began conducting an independent evaluation of the implementation of this pilot study. This phase of IPI’s study was structured exclusively around the experiences of K-3 teachers with administering and utilizing the output of the Istation IRI assessment

Trees, Forests and People, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Cancer Chemotherapy and Pharmacology, 1982

A phase II study of intermittent high-dose 6-thioguanine (6-TG) was undertaken in 19 patients wit... more A phase II study of intermittent high-dose 6-thioguanine (6-TG) was undertaken in 19 patients with metastatic colorectal carcinoma. Fourteen patients had received prior myelosuppressive therapy. 6-TG was administered as a single dose by 1V bolus over 15-30 rain, with retreatment every 3 weeks. The starting dose was 700 mg/m 2 in ten patients, 900 mg/m 2 in one patient, 1,000 mg/m 2 in four patients, and 1,200 mg/m 2 in two patients. Two patients received reduced doses (350 mg/m 2) because of liver dysfunction. There was no regression of measurable disease after treatment with 6-TG in this study. Eight patients achieved stabilization of previously progressive disease for periods of 10-32 weeks. Toxicities were nausea and vomiting (19 patients), mucositis (3 patients), reversible renal dysfunction with creatinine > 2 mg/dl (4 patients), nasal congestion (3 patients), diarrhea (1 patient), and skin blistering at the infusion site (1 patient). Seven patients had white blood count nadirs below 3,000/~tl (the lowest nadir was 900/~tl). Only one patient had a platelet count nadir below lO0,O00/~tl. There were no infections or hemorrhage. 6-TG, as administered in this study, has no antitumor activity against colorectal carcinoma. Concentrations of 6-TG and metabolites were assessed in the plasma of six patients by a reversed-phase HPLC system. 6-TG and metabolites were extracted from human plasma at 50%-100% efficiency by cold 2 N perchloric acid (1 : 1). Neutralized extracts were chromatographed on a ~t-Bondapak Cls column by two separate isocratic conditions. 6-TG, 6-thiouric acid, 6-thioguanosine, and 6-thioxanthine were analyzed with 0.01 M Na acetate, pH3.5/10% methanol as the mobile phase and were detected at 340 nm. 6-Methyl TG and three Reprint requests should be addressed to M. J. Egorin * This paper was presented in part at the 72rid Annual Meeting of the American Association for Cancer Research, Washington, DC, 1981 unknown metabolites were eluted with Na acetate~25% methanol and were detected at 310 nm. External standard calibration was used for quantitation. The 6-TG detection limit was 0.8 nmol/ml. In six patients who received 1-1.2 g 6-TG/m 2 IV, 6-TG achieved peak plasma concentrations of 61-118 nmol/ml (95.6 +_ 23.0, mean + SD). Plasma 6-TG concentrations decayed bi-exponentially, with initial tl/2 of 3 h and terminal q/2 of 5.9 h. 6-Thiouric acid, 6-methyl TG, 6-thioguanosine, 6-thioxanthine, and three major unidentified metabolites were also observed in plasma. The three unknowns were extracted with ethyl acetate from alkalinized pooled plasma extracts and were purified by HPLC.

The Professional Animal Scientist, 2015

The molecule 2-methyl-2-butenal (2M2B) is a maternal pheromone in rabbits and an interomone in do... more The molecule 2-methyl-2-butenal (2M2B) is a maternal pheromone in rabbits and an interomone in dogs eliciting behavioral and heart-rate changes. The molecule 2M2B is manufactured in dog collars (Nurturecalm 24/

The Professional Animal Scientist, 2010

Effects of 3 levels of distillers grains (DG) and 3 ratios of steam-flaked corn (SFC) to ground c... more Effects of 3 levels of distillers grains (DG) and 3 ratios of steam-flaked corn (SFC) to ground corn (GC) were used to evaluate IVDMD, in vitro gas production kinetics, and in vitro H 2 S production using ruminal fluid from 2 Jersey crossbred steers. Substrates included either corn (CDG) or sorghum distillers grains (SDG). Within each type of DG, treatments were 0, 15, or 30% DG (DM basis) and ratios of SFC:GC of 50:50, 75:25, or 100:0. With CDG, IVDMD tended (P = 0.06) to increase linearly as SFC increased in substrates, and increasing CDG increased H 2 S production (linear, P < 0.01). Fractional rate of gas production tended to decrease quadratically (P = 0.07) with decreased SFC in substrates. Molar proportion of propionate (P = 0.05) and acetate:propionate ratio (A:P; P = 0.03) responded quadratically to CDG level, with the greatest values at 15% DG. With SDG, IVDMD decreased (P = 0.02) as the percentage of SFC decreased and tended (P = 0.09) to increase as SDG level increased. Similar to CDG, H 2 S production increased linearly (P = 0.02) as SDG increased from 0 to 30% of the DM. Fractional rate of gas production was greatest (quadratic, P = 0.04) for 75:25 ratios of SFC to GC and was not affected by SDG level (P > 0.23). Total VFA concentration decreased (P = 0.03) as proportion of SFC decreased, and increasing SDG tended to decrease propionate (P = 0.09) and increase A:P (P = 0.06). Overall, increasing concentration of CDG or SDG increased in vitro H 2 S production. Effects of DG concentration were largely independent of the percentage of SFC in the substrate for IVDMD and gas production kinetics, but adding DG tended to increase A:P proportions in vitro.

The Professional Animal Scientist, 2011

Effects of substrates containing different proportions of distillers solubles (DS) to distillers ... more Effects of substrates containing different proportions of distillers solubles (DS) to distillers grain (DG) on IVDMD, H 2 S production, gas production kinetics, VFA proportions, and culture fluid osmolality were evaluated in a randomized block design with a 2 × 3 + 1 factorial arrangement of treatments. Treatment substrates consisted of 15 or 30% DG with DS added within each DG concentration to yield proportions of DG:DS of 100:0, 75:25, and 50:50; the steam-flaked corn-based control treatment contained no DG. Two ruminally cannulated Jersey crossbred steers (BW = 590 kg) fed a 60% concentrate, steam-flaked corn-based diet were used as ruminal fluid donors. Duplicate cultures were incubated for 24 h to measure IVDMD and 48 h for gas production kinetics, with incubations replicated on separate days. No differences were observed among treatments for IVDMD (P > 0.15), but H 2 S production was increased (P < 0.01) by 39 and 73% for 15 and 30% DG, respectively, relative to the control. Moreover, H 2 S production increased linearly as the proportion of DS increased (P < 0.01). Osmolality was greater for the control (P < 0.001) than for other treatments, decreased (P < 0.001) with increasing DG concentration, increased linearly (P < 0.001) with increased DS in substrates, and increased over time (P < 0.001). Fractional rate of gas production (h −1) was less (P = 0.01) for 30 versus 15% DG, and lag time of gas production decreased as DS increased in substrates (linear, P = 0.02). Molar proportion of acetate was least (P = 0.02), propionate was greatest (P < 0.01), and acetate:propionate ratio was least (P = 0.02) for the control versus other treatments. In general, including DG in substrates and increasing proportions of DS increased H 2 S production, reflecting increased S concentrations. For both 15 and 30% DG, substrates with greater proportions of DS had increased total gas production and culture fluid osmolality.

Kansas Agricultural Experiment Station Research Reports, 2008

Kansas Agricultural Experiment Station Research Reports, 2007