Mayurika Patel - Academia.edu (original) (raw)
Papers by Mayurika Patel
Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. ha... more Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. haemolyticus, the closest phylogenetic relative of H. influenzae, is arguably a strict pharyngeal commensal. A hemolytic phenotype has historically differentiated H. haemolyticus from H. influenzae, but the recent recognition of significant nonhemolytic H. haemolyticus colonization has decreased this trait's resolvability. Given this and the potential of recombination between the species, we examined the distribution of microbiologic and molecular traits between collections of H. influenzae and H. haemolyticus strains separated within a dendrogram obtained by multilocus sequence analysis (MLSA). All strains hybridizing with a probe to iga, a gene encoding an immunoglobulin A protease of H. influenzae, clustered apart from strains that did not hybridize with the probe. Other traits also segregated significantly along this division, suggesting a separation of the species. Of note, the LOS genes licA, lic2A, and lgtC of H. influenzae were approximately 2, 6, and 54 times, respectively, more prevalent in H. influenzae than in H. haemolyticus. In contrast to species separation, interspecies recombination was evidenced by the inability of single gene sequences to phylogenetically separate the species and by the "fuzzy" distribution of some species-specific traits across the species dividing line. Together, these data support the historically accurate and pragmatic division of these species while recognizing their potential for recombination. Future comparative genomic studies identifying common and distinctive genes could be useful in evaluating their role in the commensal or virulent growth, respectively, of H. influenzae.
2015 International Conference on Innovations in Information, Embedded and Communication Systems (ICIIECS), 2015
The hybrid mobile ad hoc network (H-MANET) consist of self organized mobile nodes connected over ... more The hybrid mobile ad hoc network (H-MANET) consist of self organized mobile nodes connected over radio frequency (RF) and free space optics (FSO) based wireless links. Due to limited transmission range of RF based network, the communication between two RF based MANETs are carried out by using FSO link. We have design two different clusters of RF based MANETs. Both clusters are connected by two FSO nodes, which are also a part of an individual RF based network. In our research work, we have analyze the performance of H-MANET in terms of throughput for different network areas (1000m×1000m, 2000m×2000m and 3000m×3000m) and scaling number of nodes up to 200. We have used the same protocol combinations (AODV-AODV, AOMDV-AOMDV, DSDV-DSDV and OLSR-OLSR) for two different RF based networks and joint together by FSO link for analyzing the effectiveness of H-MANET. The simulation experiment is carried out by using network simulator tool (NS 2.35) and data analysis with graphical representation has been done by using MATLAB®.
Journal of Microbiological Methods, 2008
Haemophilus haemolyticus were compared. The results of iga variable region hybridization to dotbl... more Haemophilus haemolyticus were compared. The results of iga variable region hybridization to dotblots and library-on-a-slide microarrays were more similar to a "gold standard" multigene phylogenetic tree than iga conserved region hybridization or P6 7F3 epitope immunoblots.
Journal of Clinical Microbiology, 2007
Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. ha... more Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. haemolyticus , the closest phylogenetic relative of H. influenzae , is arguably a strict pharyngeal commensal. A hemolytic phenotype has historically differentiated H. haemolyticus from H. influenzae , but the recent recognition of significant nonhemolytic H. haemolyticus colonization has decreased this trait's resolvability. Given this and the potential of recombination between the species, we examined the distribution of microbiologic and molecular traits between collections of H. influenzae and H. haemolyticus strains separated within a dendrogram obtained by multilocus sequence analysis (MLSA). All strains hybridizing with a probe to iga , a gene encoding an immunoglobulin A protease of H. influenzae , clustered apart from strains that did not hybridize with the probe. Other traits also segregated significantly along this division, suggesting a separation of the species. Of note, t...
Infection and Immunity, 2001
A subset of nontypeable Haemophilus influenzae(NTHI) biotype IV isolates from the human genital t... more A subset of nontypeable Haemophilus influenzae(NTHI) biotype IV isolates from the human genital tract or from infected newborn infants forms a cryptic genospecies characterized by, among other features, the presence of peritrichous pili. The objective of this study was to determine the similarity of these pili to hemagglutinating, HifA- and HifE-containing pili expressed by respiratory H. influenzae isolates. For this analysis, the presence of hifA and hifE and their gene products in NTHI biotype IV strains was assessed, the binding ofH. influenzae biotype IV strains to human epithelial cells was characterized, possible genital tissue tropism of these isolates was explored, and the role of HifA- and HifE-possessing pili in the adhesion of NTHI biotype IV strains to human epithelial cells was determined. None of the six biotype IV NTHI isolates tested agglutinated human red blood cells, nor could they be enriched for hemagglutinating variants. Although hifA, which encodes the major s...
Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. ha... more Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. haemolyticus, the closest phylogenetic relative of H. influenzae, is arguably a strict pharyngeal commensal. A hemolytic phenotype has historically differentiated H. haemolyticus from H. influenzae, but the recent recognition of significant nonhemolytic H. haemolyticus colonization has decreased this trait's resolvability. Given this and the potential of recombination between the species, we examined the distribution of microbiologic and molecular traits between collections of H. influenzae and H. haemolyticus strains separated within a dendrogram obtained by multilocus sequence analysis (MLSA). All strains hybridizing with a probe to iga, a gene encoding an immunoglobulin A protease of H. influenzae, clustered apart from strains that did not hybridize with the probe. Other traits also segregated significantly along this division, suggesting a separation of the species. Of note, the LOS genes licA, lic2A, and lgtC of H. influenzae were approximately 2, 6, and 54 times, respectively, more prevalent in H. influenzae than in H. haemolyticus. In contrast to species separation, interspecies recombination was evidenced by the inability of single gene sequences to phylogenetically separate the species and by the "fuzzy" distribution of some species-specific traits across the species dividing line. Together, these data support the historically accurate and pragmatic division of these species while recognizing their potential for recombination. Future comparative genomic studies identifying common and distinctive genes could be useful in evaluating their role in the commensal or virulent growth, respectively, of H. influenzae.
2015 International Conference on Innovations in Information, Embedded and Communication Systems (ICIIECS), 2015
The hybrid mobile ad hoc network (H-MANET) consist of self organized mobile nodes connected over ... more The hybrid mobile ad hoc network (H-MANET) consist of self organized mobile nodes connected over radio frequency (RF) and free space optics (FSO) based wireless links. Due to limited transmission range of RF based network, the communication between two RF based MANETs are carried out by using FSO link. We have design two different clusters of RF based MANETs. Both clusters are connected by two FSO nodes, which are also a part of an individual RF based network. In our research work, we have analyze the performance of H-MANET in terms of throughput for different network areas (1000m×1000m, 2000m×2000m and 3000m×3000m) and scaling number of nodes up to 200. We have used the same protocol combinations (AODV-AODV, AOMDV-AOMDV, DSDV-DSDV and OLSR-OLSR) for two different RF based networks and joint together by FSO link for analyzing the effectiveness of H-MANET. The simulation experiment is carried out by using network simulator tool (NS 2.35) and data analysis with graphical representation has been done by using MATLAB®.
Journal of Microbiological Methods, 2008
Haemophilus haemolyticus were compared. The results of iga variable region hybridization to dotbl... more Haemophilus haemolyticus were compared. The results of iga variable region hybridization to dotblots and library-on-a-slide microarrays were more similar to a "gold standard" multigene phylogenetic tree than iga conserved region hybridization or P6 7F3 epitope immunoblots.
Journal of Clinical Microbiology, 2007
Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. ha... more Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. haemolyticus , the closest phylogenetic relative of H. influenzae , is arguably a strict pharyngeal commensal. A hemolytic phenotype has historically differentiated H. haemolyticus from H. influenzae , but the recent recognition of significant nonhemolytic H. haemolyticus colonization has decreased this trait's resolvability. Given this and the potential of recombination between the species, we examined the distribution of microbiologic and molecular traits between collections of H. influenzae and H. haemolyticus strains separated within a dendrogram obtained by multilocus sequence analysis (MLSA). All strains hybridizing with a probe to iga , a gene encoding an immunoglobulin A protease of H. influenzae , clustered apart from strains that did not hybridize with the probe. Other traits also segregated significantly along this division, suggesting a separation of the species. Of note, t...
Infection and Immunity, 2001
A subset of nontypeable Haemophilus influenzae(NTHI) biotype IV isolates from the human genital t... more A subset of nontypeable Haemophilus influenzae(NTHI) biotype IV isolates from the human genital tract or from infected newborn infants forms a cryptic genospecies characterized by, among other features, the presence of peritrichous pili. The objective of this study was to determine the similarity of these pili to hemagglutinating, HifA- and HifE-containing pili expressed by respiratory H. influenzae isolates. For this analysis, the presence of hifA and hifE and their gene products in NTHI biotype IV strains was assessed, the binding ofH. influenzae biotype IV strains to human epithelial cells was characterized, possible genital tissue tropism of these isolates was explored, and the role of HifA- and HifE-possessing pili in the adhesion of NTHI biotype IV strains to human epithelial cells was determined. None of the six biotype IV NTHI isolates tested agglutinated human red blood cells, nor could they be enriched for hemagglutinating variants. Although hifA, which encodes the major s...