Md. Atiqur Rahman Khokon - Academia.edu (original) (raw)

Papers by Md. Atiqur Rahman Khokon

Heliyon

Production of brinjal (Solanum melongena L.) is considerably reduced by infestations of root-knot... more Production of brinjal (Solanum melongena L.) is considerably reduced by infestations of root-knot nematodes (RKN). As chemical pesticides are increasingly being regulated globally, scientists are focusing on biorational management. An experiment was undertaken to screen resistant brinjal cultivars in Bangladesh against Meloidogyne javanica in a pot trial. Pot and field trials were also conducted to evaluate the efficacy and profitability of individual and combined applications of several biorational components to manage M. javanica on brinjal. Of twenty brinjal cultivars screened, cv. Noagram was found 'moderately resistant' and others were 'susceptible' to 'highly susceptible' against M. javanica. In both pot and field trials, most of the growth parameters of brinjal and reproductive parameters of M. javanica were significantly different than the control for both the individual and combined treatments of different biorational components which included cabbage, marigold, vermicompost, biogas digestate, Bacillus subtilis and Pseudomonas fluorescens. The yield was significantly higher for the combined treatments than the individual applications. The benefit-cost ratio (BCR) differed among the treatments. The highest yield (29.5 t/ha) and BCR (3.67) with the lowest reproductive factor (0.33) was obtained by the combined application of biogas digestate and B. subtilis. This is the first report on the efficiency and profitability assessment of biogas digestate in combination with a bio-agent in addressing the management of RKN, which might be very important considering the global concern of environmental pollution. The cultivar Noagram might be a potential source of resistant genes in brinjal against M. javanica.

Journal of Bangladesh Agricultural University, 2020

Culture filtrates collected from Trichoderma harzianum were assayed to control seed-borne fungi o... more Culture filtrates collected from Trichoderma harzianum were assayed to control seed-borne fungi of selected vegetables viz. bottle gourd, wax gourd, sweet gourd, snake gourd and okra. Three fungal genera comprising of four species viz. Aspergillus niger, Aspergillus flavus, Penicillium spp. and Fusarium oxysporum were found to be associated with most of the vegetable seeds. Among the seed borne fungi the highest incidence was recorded in case of A. niger (49.2%) followed by A. flavus (40.6%) and F. oxysporum (40.2%). Five treatments viz. 1%, 3%, 5%, 7% aqueous solution of culture filtrates and control (without culture filtrate) were assayed against individual seed-borne fungal pathogen following poison food technique. Among the treatments, 7% aqueous solution of culture filtrate showed superior performance by suppressing mycelial growth of all fungal genera and this treatment was further considered to treat the seeds to control seed borne fungi. Prevalence of seedborne A. niger, A. flavus, Penicillium spp. and F. oxysporum were reduced significantly by seed treatment with 7% aqueous solution of culture filtrates. The findings of the study suggested that seed priming by aqueous solution of culture filtrates of Trichoderma (7%) can be used for vegetable seed treatment for controlling seed-borne fungal infection.

been through the copyediting, typesetting, pagination and proofreading process, which may lead to... more been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/plb.12321 This article is protected by copyright. All rights reserved. Received Date : 08-Dec-2014 Revised Date : 01-Feb-2015 Accepted Date : 06-Feb-2015 Article type : Research Paper Editor : R Mendel

International Journal of Sustainable Agricultural Technology, 2011

Archives of Phytopathology and Plant Protection, 2021

Abstract Brinjal (Solanum melongena L.) is an important vegetable worldwide and its production is... more Abstract Brinjal (Solanum melongena L.) is an important vegetable worldwide and its production is hampered by the infestation of root-knot nematodes (Meloidogyne spp.). In an attempt to identify the Meloidogyne species involved in causing root-knot disease of brinjal by using molecular tools, plants showing knots/galls were collected from 22 brinjal growing locations that belong to different agro-ecological zones in Bangladesh. Nematode populations were maintained in a susceptible brinjal variety by inoculating with egg-masses developed on different varieties of brinjal roots collected from different locations. For molecular identification, DNA was extracted directly from galls developed in the roots of the inoculated plants. Randomly selected three galls from each location were crushed together in order to get DNA from mixed species of root-knot nematodes. Sequence Specific Amplified Region (SCAR) primers specific for Meloidogyne incognita, M. javanica or M. arenaria were used for PCR amplification. For 18 of the 22 locations, M. javanica was identified. At two locations M. incognita was found as a mixed population with M. javanica. M. arenaria was not found. PCR products were sequenced bi-directionally and their analysis revealed genetic variation among the populations. M. javanica was the predominant root-knot nematode species of brinjal in Bangladesh. Intra-specific variation of the SCAR suggested six phylogenetic groups of M. javanica. They showed significant differences in reproduction on brinjal and in their effect on root and shoot weight, while differences in hatching dynamics over five weeks were not significant. This population-dependent aggressiveness of the dominant root-knot nematode M. javanica on brinjal in Bangladesh should be considered for a more efficient management.

Saudi Journal of Biological Sciences, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Saudi Journal of Biological Sciences, 2020

Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biologi... more Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biological control agents against root-knot nematodes (RKN) of different crops. However, their performance as biocontrol agents varies with their place of origin. Culture filtrates of rhizospheric bacteria contain some intermediary metabolites that have nematicidal activity. An in vitro experiment was undertaken to evaluate the functionality of culture filtrates of B. subtilis (MN252542.1) and P. fluorescens (MN256394.1) at different concentrations (1.0%, 2.5%, 5.0%, 7.0%, 10.0% and 25.0%) on the hatching and mortality of Meloidogyne javanica at different time span. Bacterial strains were isolated from rhizospheric soils of Bangladesh. At three days after incubation (DAI), 25.0% concentration of culture filtrates of both B. subtilis and P. fluorescens showed 100.0% mortality of second stage juveniles (J 2) of M. javanica. Additionally, 25.0% concentration of culture filtrates of both bacteria showed 100.0% inhibition of hatching at one week after incubation (WAI). A decreasing trend in hatching of M. javanica was observed with the increment of the concentration of culture filtrates and progression of incubation time. The findings of this experiment reveal that culture filtrates of these accessions of B. subtilis and P. fluorescens are effective for controlling M. javanica and would be potential candidates for developing bio-nematicides.

Universal Journal of Plant Science, 2018

A field experiment was conducted to investigate the efficacy of Trichocompost utilizing Trichoder... more A field experiment was conducted to investigate the efficacy of Trichocompost utilizing Trichoderma as biological agent. Three doses of Trichocompost viz. 500g/m 2 , 750g/m 2 , 1000g/m 2 were applied including untreated control treatment. All the yield parameters were significantly higher when they were applied in different doses of Trichocompost. However, growth parameters did not show significant variation although luxuriant and lavish growth was noticed. Best performances were recorded by applying Trichocompost application @1000g/m 2. Economic analysis of the treatments indicates that Trichocompost is profitable for farmers. Benefit Cost Ratio (BCR) was maximum (2.26) in Trichocompost treated plot @1000g/m 2. It can be revealed that Trichocompost can be applied in the field for better tomato production.

Journal of the Bangladesh Agricultural University, 2018

Twelve isolates of Colletotrichum capsici and Colletotrichum gloeosporides were isolated from ant... more Twelve isolates of Colletotrichum capsici and Colletotrichum gloeosporides were isolated from anthracnose infected chilli fruits from different areas of Mymensingh. Isolated pure fungal isolates were grouped on the basis of their morphological characters viz. colony color and compactness, size, shape and number of conidia. The white colored isolates were identified as C. capsici and showed faster growth on PDA medium. In contrast, grey colored fungal isolates were identified as C. gloeosporides and comparatively slow growth on PDA medium. All the fungal isolates were pathogenically active and developed typical symptoms on both green and ripe fruits of chilli. The isolates of C. capsici collected from Kalibari showed the highest infection (74.99%) on fruit surface followed by Muktijoddhar bazar (61.83%). Differential tolerance was observed as fungal growth was different against 0.05% and 0.1% carbendazim while 0.2% carbendazim was lethal against all fungal isolates. The results indic...

Seed Science and Technology, 2005

Current Agriculture Research Journal, 2018

Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating dise... more Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating diseases of mustard-rapeseed in Bangladesh, and can cause yield loss up to 60% every year. A total of 27 cultivars of Brassica spp. where Brassica rapa (13), B. juncea (5) and B. napus (9) were selected for resistance screening against A. brassicicola. Two inoculation methods viz. detached leaf and seedling inoculation were assessed for checking the validation of inoculation technique using cultivated mustard-rapeseed varieties in Bangladesh a method to measure resistance to A. brassicicola. A significant positive correlation between the results of two inoculation methods was found in this study. The detached leaf technique was more suitable due to development of clear symptoms on the leaves within 36 hrs, and suitable for screening large scale genotypes for resistance. The 3rd leaves of 30 day-old were more suitable for inoculation having severe symptoms than the 4th leaves. Among 27 Bangla...

Azad, M. R., Alam, M. Z., Hossain, M. A. and Khokon, M. A. R. 2017. Effect of biofungicide on the... more Azad, M. R., Alam, M. Z., Hossain, M. A. and Khokon, M. A. R. 2017. Effect of biofungicide on the production of healthy and quality seeds of Salvia hispanica in Bangladesh. Bangladesh J. Plant Pathol. 33 (1&2): 57-64 Experiments were conducted both in the field and laboratory to evaluate the roles of Trichoderma-based biofungicide for improving the health and quality of chia seeds. Four treatments viz. Control-untreated, Seeds treated with BAU-Biofungicide @ 2% of seed weight, Soil treated with IPM lab Biopesticide @ 64kg/ha and Seeds treated with BAU-Biofungicide @ 2% of seed weight + Soil treated with IPMBiopesticide @ 64kg/ha were used in the field experiment. Among the treatments, BAUBiofungicide showed better performance in terms of germination percentage, no. of branches, plant height, and seed yield. Both BAU-Biofungicide and BAUBiofungicide + IPM lab Biopesticide performed better in terms of germination percentage on blotter paper. The identified seed borne fungi were Fusari...

Archives of Phytopathology and Plant Protection, 2020

Fluorescent Pseudomonads (Pseudomonas fluorescens and P. putida) were formulated in talc, bentoni... more Fluorescent Pseudomonads (Pseudomonas fluorescens and P. putida) were formulated in talc, bentonite, palm oil and coconut oil for the management of blast disease of rice. The formulated products showed promising shelf-value. The formulated products were evaluated by root dipping of rice seedlings and foliar spray followed by inoculation with M. oryzae in pots. All growth parameters, yield parameters, incidence and severity of blast or rice were significantly influenced at every growth stage of rice. Yield contributing parameters were increased significantly giving the highest benefit cost ratio (BCR) compared to untreated control. Among the treatments, T11 (10% BdPf-8 Talc) and T13 (1% BdPf-8 Palm Oil) gave the highest yield 5.18 t/ha and 5.13 t/ha with the highest BCR 2.10 and 2.08, respectively. A negative correlation was observed among vegetative parameters, disease incidence and disease severity. Pseudomonas fluorescens (BdPf-8) can be a potential candidate for commercial produc...

Journal of the Bangladesh Agricultural University

ARTICLE INFO Experiments were conducted under laboratory condition to examine the efficacy of Chi... more ARTICLE INFO Experiments were conducted under laboratory condition to examine the efficacy of Chitosan and Yeast Elicitor to suppress the growth of seed-borne fungi of cucurbitaceous vegetables. Seeds of bottle gourd, sweet gourd, snake gourd, wax gourd and cucumber were collected from seed traders of Mymensingh districts and different seed borne fungi were isolated, purified and identified. Fourteen fungal species belonging to twelve genera consisting of Aspergillus flavus, Aspergillus niger, Botrytis cinerea, Fusarium moniliforme, Fusarium oxysporum, Phoma exigua, Rhizopus stolonifer, Macrophomina phaseolina, Penicillium spp., Curvularia lunata, Chaetomium spp., Colletotrichum spp., Cercospora spp. and Alternaria alternata were isolated and identified. Four concentrations of Chitosan and Yeast Elicitors solutions (200, 500, 1000 & 2000 ppm) including one positive control Vitavax-200 WP (0.35%) were evaluated for controlling seed-borne fungi. Among the seed treating agents Chitosan (2000 ppm) and Yeast Elicitor (2000 ppm) showed better performance in suppressing the seed-borne fungi. Chitosan (2000 ppm) showed superior performance than Yeast Elicitor (2000 ppm). Results from the present study revealed that application of elicitors as seed treatment is a potential alternative of chemical fungicide for selective vegetables.

Bioscience, Biotechnology, and Biochemistry

Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) ... more Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutan...

Pakistan Journal of Biological Sciences, 2001

... Statistical Procedure for Agricultural Research. John Wiley and Sons, New York, 400-620 Ingra... more ... Statistical Procedure for Agricultural Research. John Wiley and Sons, New York, 400-620 Ingram, DH and PH Williams, 1971. Advances in Plant Pathology. Academic press. ... Chapman and Hall Ltd. NY. pp: 461-462 Zahid, MI, KD Tapan and BC Chowdhury, 1993. ...

Pakistan Journal of Biological Sciences, 2001

... 4 (12): 1500-1502, 2001 © Asian Network for Scientific Information 2001 Jute Leaf Mosaic and ... more ... 4 (12): 1500-1502, 2001 © Asian Network for Scientific Information 2001 Jute Leaf Mosaic and its Effects on Jute Production 1 Sukalpa Das, Md. ... Cassette holder method (Shakya and Chung, 1983) was also used for germination test and to observe the early seedling symptoms. ...

Bioscience Biotechnology and Biochemistry, Jan 21, 2015

Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced ... more Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited lightinduced stomatal opening in a dose-dependent manner. Acrolein at 100 μM inhibited plasma membrane inward-rectifying potassium (K in ) channels in guard cells. Acrolein at 100 μM inhibited K in channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of K in channels in guard cells.

Http Dx Doi Org 10 1271 Bbb 100340, May 22, 2014

ABSTRACT

Plant Biology, 2015

Methyl jasmonate (MeJA) and abscisic acid (ABA) signaling cascades share several signaling compon... more Methyl jasmonate (MeJA) and abscisic acid (ABA) signaling cascades share several signaling components in guard cells. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signaling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in the wild-type plants. MeJA elicited ROS production and cytosolic alkalization in guard cells of the mpk9-1, mpk12-1, and mpk9-1 mpk12-1 mutants as well as those of the wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca(2+) concentration ([Ca(2+) ]cyt ) in the mpk9-1 mpk12-1 double mutant as well as the wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Altogether, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalization, and [Ca(2+) ]cyt elevation in guard cell MeJA signaling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signaling in guard cells. This article is protected by copyright. All rights reserved.

Heliyon

Production of brinjal (Solanum melongena L.) is considerably reduced by infestations of root-knot... more Production of brinjal (Solanum melongena L.) is considerably reduced by infestations of root-knot nematodes (RKN). As chemical pesticides are increasingly being regulated globally, scientists are focusing on biorational management. An experiment was undertaken to screen resistant brinjal cultivars in Bangladesh against Meloidogyne javanica in a pot trial. Pot and field trials were also conducted to evaluate the efficacy and profitability of individual and combined applications of several biorational components to manage M. javanica on brinjal. Of twenty brinjal cultivars screened, cv. Noagram was found 'moderately resistant' and others were 'susceptible' to 'highly susceptible' against M. javanica. In both pot and field trials, most of the growth parameters of brinjal and reproductive parameters of M. javanica were significantly different than the control for both the individual and combined treatments of different biorational components which included cabbage, marigold, vermicompost, biogas digestate, Bacillus subtilis and Pseudomonas fluorescens. The yield was significantly higher for the combined treatments than the individual applications. The benefit-cost ratio (BCR) differed among the treatments. The highest yield (29.5 t/ha) and BCR (3.67) with the lowest reproductive factor (0.33) was obtained by the combined application of biogas digestate and B. subtilis. This is the first report on the efficiency and profitability assessment of biogas digestate in combination with a bio-agent in addressing the management of RKN, which might be very important considering the global concern of environmental pollution. The cultivar Noagram might be a potential source of resistant genes in brinjal against M. javanica.

Journal of Bangladesh Agricultural University, 2020

Culture filtrates collected from Trichoderma harzianum were assayed to control seed-borne fungi o... more Culture filtrates collected from Trichoderma harzianum were assayed to control seed-borne fungi of selected vegetables viz. bottle gourd, wax gourd, sweet gourd, snake gourd and okra. Three fungal genera comprising of four species viz. Aspergillus niger, Aspergillus flavus, Penicillium spp. and Fusarium oxysporum were found to be associated with most of the vegetable seeds. Among the seed borne fungi the highest incidence was recorded in case of A. niger (49.2%) followed by A. flavus (40.6%) and F. oxysporum (40.2%). Five treatments viz. 1%, 3%, 5%, 7% aqueous solution of culture filtrates and control (without culture filtrate) were assayed against individual seed-borne fungal pathogen following poison food technique. Among the treatments, 7% aqueous solution of culture filtrate showed superior performance by suppressing mycelial growth of all fungal genera and this treatment was further considered to treat the seeds to control seed borne fungi. Prevalence of seedborne A. niger, A. flavus, Penicillium spp. and F. oxysporum were reduced significantly by seed treatment with 7% aqueous solution of culture filtrates. The findings of the study suggested that seed priming by aqueous solution of culture filtrates of Trichoderma (7%) can be used for vegetable seed treatment for controlling seed-borne fungal infection.

been through the copyediting, typesetting, pagination and proofreading process, which may lead to... more been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/plb.12321 This article is protected by copyright. All rights reserved. Received Date : 08-Dec-2014 Revised Date : 01-Feb-2015 Accepted Date : 06-Feb-2015 Article type : Research Paper Editor : R Mendel

International Journal of Sustainable Agricultural Technology, 2011

Archives of Phytopathology and Plant Protection, 2021

Abstract Brinjal (Solanum melongena L.) is an important vegetable worldwide and its production is... more Abstract Brinjal (Solanum melongena L.) is an important vegetable worldwide and its production is hampered by the infestation of root-knot nematodes (Meloidogyne spp.). In an attempt to identify the Meloidogyne species involved in causing root-knot disease of brinjal by using molecular tools, plants showing knots/galls were collected from 22 brinjal growing locations that belong to different agro-ecological zones in Bangladesh. Nematode populations were maintained in a susceptible brinjal variety by inoculating with egg-masses developed on different varieties of brinjal roots collected from different locations. For molecular identification, DNA was extracted directly from galls developed in the roots of the inoculated plants. Randomly selected three galls from each location were crushed together in order to get DNA from mixed species of root-knot nematodes. Sequence Specific Amplified Region (SCAR) primers specific for Meloidogyne incognita, M. javanica or M. arenaria were used for PCR amplification. For 18 of the 22 locations, M. javanica was identified. At two locations M. incognita was found as a mixed population with M. javanica. M. arenaria was not found. PCR products were sequenced bi-directionally and their analysis revealed genetic variation among the populations. M. javanica was the predominant root-knot nematode species of brinjal in Bangladesh. Intra-specific variation of the SCAR suggested six phylogenetic groups of M. javanica. They showed significant differences in reproduction on brinjal and in their effect on root and shoot weight, while differences in hatching dynamics over five weeks were not significant. This population-dependent aggressiveness of the dominant root-knot nematode M. javanica on brinjal in Bangladesh should be considered for a more efficient management.

Saudi Journal of Biological Sciences, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Saudi Journal of Biological Sciences, 2020

Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biologi... more Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biological control agents against root-knot nematodes (RKN) of different crops. However, their performance as biocontrol agents varies with their place of origin. Culture filtrates of rhizospheric bacteria contain some intermediary metabolites that have nematicidal activity. An in vitro experiment was undertaken to evaluate the functionality of culture filtrates of B. subtilis (MN252542.1) and P. fluorescens (MN256394.1) at different concentrations (1.0%, 2.5%, 5.0%, 7.0%, 10.0% and 25.0%) on the hatching and mortality of Meloidogyne javanica at different time span. Bacterial strains were isolated from rhizospheric soils of Bangladesh. At three days after incubation (DAI), 25.0% concentration of culture filtrates of both B. subtilis and P. fluorescens showed 100.0% mortality of second stage juveniles (J 2) of M. javanica. Additionally, 25.0% concentration of culture filtrates of both bacteria showed 100.0% inhibition of hatching at one week after incubation (WAI). A decreasing trend in hatching of M. javanica was observed with the increment of the concentration of culture filtrates and progression of incubation time. The findings of this experiment reveal that culture filtrates of these accessions of B. subtilis and P. fluorescens are effective for controlling M. javanica and would be potential candidates for developing bio-nematicides.

Universal Journal of Plant Science, 2018

A field experiment was conducted to investigate the efficacy of Trichocompost utilizing Trichoder... more A field experiment was conducted to investigate the efficacy of Trichocompost utilizing Trichoderma as biological agent. Three doses of Trichocompost viz. 500g/m 2 , 750g/m 2 , 1000g/m 2 were applied including untreated control treatment. All the yield parameters were significantly higher when they were applied in different doses of Trichocompost. However, growth parameters did not show significant variation although luxuriant and lavish growth was noticed. Best performances were recorded by applying Trichocompost application @1000g/m 2. Economic analysis of the treatments indicates that Trichocompost is profitable for farmers. Benefit Cost Ratio (BCR) was maximum (2.26) in Trichocompost treated plot @1000g/m 2. It can be revealed that Trichocompost can be applied in the field for better tomato production.

Journal of the Bangladesh Agricultural University, 2018

Twelve isolates of Colletotrichum capsici and Colletotrichum gloeosporides were isolated from ant... more Twelve isolates of Colletotrichum capsici and Colletotrichum gloeosporides were isolated from anthracnose infected chilli fruits from different areas of Mymensingh. Isolated pure fungal isolates were grouped on the basis of their morphological characters viz. colony color and compactness, size, shape and number of conidia. The white colored isolates were identified as C. capsici and showed faster growth on PDA medium. In contrast, grey colored fungal isolates were identified as C. gloeosporides and comparatively slow growth on PDA medium. All the fungal isolates were pathogenically active and developed typical symptoms on both green and ripe fruits of chilli. The isolates of C. capsici collected from Kalibari showed the highest infection (74.99%) on fruit surface followed by Muktijoddhar bazar (61.83%). Differential tolerance was observed as fungal growth was different against 0.05% and 0.1% carbendazim while 0.2% carbendazim was lethal against all fungal isolates. The results indic...

Seed Science and Technology, 2005

Current Agriculture Research Journal, 2018

Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating dise... more Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating diseases of mustard-rapeseed in Bangladesh, and can cause yield loss up to 60% every year. A total of 27 cultivars of Brassica spp. where Brassica rapa (13), B. juncea (5) and B. napus (9) were selected for resistance screening against A. brassicicola. Two inoculation methods viz. detached leaf and seedling inoculation were assessed for checking the validation of inoculation technique using cultivated mustard-rapeseed varieties in Bangladesh a method to measure resistance to A. brassicicola. A significant positive correlation between the results of two inoculation methods was found in this study. The detached leaf technique was more suitable due to development of clear symptoms on the leaves within 36 hrs, and suitable for screening large scale genotypes for resistance. The 3rd leaves of 30 day-old were more suitable for inoculation having severe symptoms than the 4th leaves. Among 27 Bangla...

Azad, M. R., Alam, M. Z., Hossain, M. A. and Khokon, M. A. R. 2017. Effect of biofungicide on the... more Azad, M. R., Alam, M. Z., Hossain, M. A. and Khokon, M. A. R. 2017. Effect of biofungicide on the production of healthy and quality seeds of Salvia hispanica in Bangladesh. Bangladesh J. Plant Pathol. 33 (1&2): 57-64 Experiments were conducted both in the field and laboratory to evaluate the roles of Trichoderma-based biofungicide for improving the health and quality of chia seeds. Four treatments viz. Control-untreated, Seeds treated with BAU-Biofungicide @ 2% of seed weight, Soil treated with IPM lab Biopesticide @ 64kg/ha and Seeds treated with BAU-Biofungicide @ 2% of seed weight + Soil treated with IPMBiopesticide @ 64kg/ha were used in the field experiment. Among the treatments, BAUBiofungicide showed better performance in terms of germination percentage, no. of branches, plant height, and seed yield. Both BAU-Biofungicide and BAUBiofungicide + IPM lab Biopesticide performed better in terms of germination percentage on blotter paper. The identified seed borne fungi were Fusari...

Archives of Phytopathology and Plant Protection, 2020

Fluorescent Pseudomonads (Pseudomonas fluorescens and P. putida) were formulated in talc, bentoni... more Fluorescent Pseudomonads (Pseudomonas fluorescens and P. putida) were formulated in talc, bentonite, palm oil and coconut oil for the management of blast disease of rice. The formulated products showed promising shelf-value. The formulated products were evaluated by root dipping of rice seedlings and foliar spray followed by inoculation with M. oryzae in pots. All growth parameters, yield parameters, incidence and severity of blast or rice were significantly influenced at every growth stage of rice. Yield contributing parameters were increased significantly giving the highest benefit cost ratio (BCR) compared to untreated control. Among the treatments, T11 (10% BdPf-8 Talc) and T13 (1% BdPf-8 Palm Oil) gave the highest yield 5.18 t/ha and 5.13 t/ha with the highest BCR 2.10 and 2.08, respectively. A negative correlation was observed among vegetative parameters, disease incidence and disease severity. Pseudomonas fluorescens (BdPf-8) can be a potential candidate for commercial produc...

Journal of the Bangladesh Agricultural University

ARTICLE INFO Experiments were conducted under laboratory condition to examine the efficacy of Chi... more ARTICLE INFO Experiments were conducted under laboratory condition to examine the efficacy of Chitosan and Yeast Elicitor to suppress the growth of seed-borne fungi of cucurbitaceous vegetables. Seeds of bottle gourd, sweet gourd, snake gourd, wax gourd and cucumber were collected from seed traders of Mymensingh districts and different seed borne fungi were isolated, purified and identified. Fourteen fungal species belonging to twelve genera consisting of Aspergillus flavus, Aspergillus niger, Botrytis cinerea, Fusarium moniliforme, Fusarium oxysporum, Phoma exigua, Rhizopus stolonifer, Macrophomina phaseolina, Penicillium spp., Curvularia lunata, Chaetomium spp., Colletotrichum spp., Cercospora spp. and Alternaria alternata were isolated and identified. Four concentrations of Chitosan and Yeast Elicitors solutions (200, 500, 1000 & 2000 ppm) including one positive control Vitavax-200 WP (0.35%) were evaluated for controlling seed-borne fungi. Among the seed treating agents Chitosan (2000 ppm) and Yeast Elicitor (2000 ppm) showed better performance in suppressing the seed-borne fungi. Chitosan (2000 ppm) showed superior performance than Yeast Elicitor (2000 ppm). Results from the present study revealed that application of elicitors as seed treatment is a potential alternative of chemical fungicide for selective vegetables.

Bioscience, Biotechnology, and Biochemistry

Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) ... more Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutan...

Pakistan Journal of Biological Sciences, 2001

... Statistical Procedure for Agricultural Research. John Wiley and Sons, New York, 400-620 Ingra... more ... Statistical Procedure for Agricultural Research. John Wiley and Sons, New York, 400-620 Ingram, DH and PH Williams, 1971. Advances in Plant Pathology. Academic press. ... Chapman and Hall Ltd. NY. pp: 461-462 Zahid, MI, KD Tapan and BC Chowdhury, 1993. ...

Pakistan Journal of Biological Sciences, 2001

... 4 (12): 1500-1502, 2001 © Asian Network for Scientific Information 2001 Jute Leaf Mosaic and ... more ... 4 (12): 1500-1502, 2001 © Asian Network for Scientific Information 2001 Jute Leaf Mosaic and its Effects on Jute Production 1 Sukalpa Das, Md. ... Cassette holder method (Shakya and Chung, 1983) was also used for germination test and to observe the early seedling symptoms. ...

Bioscience Biotechnology and Biochemistry, Jan 21, 2015

Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced ... more Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited lightinduced stomatal opening in a dose-dependent manner. Acrolein at 100 μM inhibited plasma membrane inward-rectifying potassium (K in ) channels in guard cells. Acrolein at 100 μM inhibited K in channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of K in channels in guard cells.

Http Dx Doi Org 10 1271 Bbb 100340, May 22, 2014

ABSTRACT

Plant Biology, 2015

Methyl jasmonate (MeJA) and abscisic acid (ABA) signaling cascades share several signaling compon... more Methyl jasmonate (MeJA) and abscisic acid (ABA) signaling cascades share several signaling components in guard cells. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signaling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in the wild-type plants. MeJA elicited ROS production and cytosolic alkalization in guard cells of the mpk9-1, mpk12-1, and mpk9-1 mpk12-1 mutants as well as those of the wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca(2+) concentration ([Ca(2+) ]cyt ) in the mpk9-1 mpk12-1 double mutant as well as the wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Altogether, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalization, and [Ca(2+) ]cyt elevation in guard cell MeJA signaling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signaling in guard cells. This article is protected by copyright. All rights reserved.