Md.Rezuanul Haque - Academia.edu (original) (raw)
Papers by Md.Rezuanul Haque
Author(s): Haque, Md Rezuanul | Advisor(s): Park, B. H | Abstract: Current methods for investigat... more Author(s): Haque, Md Rezuanul | Advisor(s): Park, B. H | Abstract: Current methods for investigating activity in neurons largely rely on different varieties of electrodes, which require direct or very close contact, or fluorescence-based techniques, which require chemical or genetic introduction of a voltage- or calcium-sensitive fluorophore. The primary motivation for this work was to develop an intensity (non-phase) based extension of optical coherence tomography (OCT), an optical technology capable of rapid imaging of subsurface tissue structure with micrometer resolution without the need for exogenous contrast agents, for in vitro detection of neural activity. First, a preliminary study of epileptiform activity in murine hippocampal brain slices was used as an assessment of OCT for in vitro detection of neural activity. Elevated potassium was used to induce seizure-like bursts of activity. Decreases in OCT light intensity were found to be correlated with the initial onset of lar...
Optics in the Life Sciences, 2015
We detected transient structural changes of neuron in seizure using phase-resolved spectral domai... more We detected transient structural changes of neuron in seizure using phase-resolved spectral domain optical coherence tomography by correlating changes in optical phase with electrophysiology using a multi-electrode array in a murine hippocampal brain slice.
A non-contact optical method for minimally invasive neural recording is designed and implemented.... more A non-contact optical method for minimally invasive neural recording is designed and implemented. This method is based on phase-resolved spectral domain optical coherence tomography (SD-OCT). The optical electrode system is calibrated by performing phase measurements from a glass slide and some preliminary experiments of optical imaging and phase measurements in optic nerve of Limulus polyphemus (Horseshoe Crab) have been done. With all these results, simultaneously obtained electrophysiology recording data will also be presented.
![Research paper thumbnail of OCT intensity and phase fluctuations correlated with activity-dependent neuronal calcium dynamics in the Drosophila CNS [Invited]](https://attachments.academia-assets.com/73726459/thumbnails/1.jpg)
](Biomedical Optics Express, 2017
Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic pa... more Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic patterns of neural activity in the isolated Drosophila central nervous system. Both imaging modalities were focused on individually identified bursicon neurons known to be involved in a fixed action pattern initiated by ecdysis-triggering hormone. We observed clear correspondence of OCT intensity, phase fluctuations, and activity-dependent calcium-induced fluorescence.
Optics in the Life Sciences, 2015
Current technologies for monitoring neural activity either use different variety of electrodes (e... more Current technologies for monitoring neural activity either use different variety of electrodes (electrical recording) or require contrast agents introduced exogenously or through genetic modification (optical imaging). Here we demonstrate an optical method for non-contact and contrast agent free detection of nerve activity using phase-resolved optical coherence tomography (pr-OCT). A common-path variation of the pr-OCT is recently implemented and the developed system demonstrated the capability to detect rapid transient structural changes that accompany neural spike propagation. No averaging over multiple trials was required, indicating its capability of single-shot detection of individual impulses from functionally stimulated Limulus optic nerve. The strength of this OCT-based optical electrode is that it is a contactless method and does not require any exogenous contrast agent. With further improvements in accuracy and sensitivity, this optical electrode will play a complementary ...
Author(s): Haque, Md Rezuanul | Advisor(s): Park, B. H | Abstract: Current methods for investigat... more Author(s): Haque, Md Rezuanul | Advisor(s): Park, B. H | Abstract: Current methods for investigating activity in neurons largely rely on different varieties of electrodes, which require direct or very close contact, or fluorescence-based techniques, which require chemical or genetic introduction of a voltage- or calcium-sensitive fluorophore. The primary motivation for this work was to develop an intensity (non-phase) based extension of optical coherence tomography (OCT), an optical technology capable of rapid imaging of subsurface tissue structure with micrometer resolution without the need for exogenous contrast agents, for in vitro detection of neural activity. First, a preliminary study of epileptiform activity in murine hippocampal brain slices was used as an assessment of OCT for in vitro detection of neural activity. Elevated potassium was used to induce seizure-like bursts of activity. Decreases in OCT light intensity were found to be correlated with the initial onset of lar...
Optics in the Life Sciences, 2015
We detected transient structural changes of neuron in seizure using phase-resolved spectral domai... more We detected transient structural changes of neuron in seizure using phase-resolved spectral domain optical coherence tomography by correlating changes in optical phase with electrophysiology using a multi-electrode array in a murine hippocampal brain slice.
A non-contact optical method for minimally invasive neural recording is designed and implemented.... more A non-contact optical method for minimally invasive neural recording is designed and implemented. This method is based on phase-resolved spectral domain optical coherence tomography (SD-OCT). The optical electrode system is calibrated by performing phase measurements from a glass slide and some preliminary experiments of optical imaging and phase measurements in optic nerve of Limulus polyphemus (Horseshoe Crab) have been done. With all these results, simultaneously obtained electrophysiology recording data will also be presented.
Biomedical Optics Express, 2017
Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic pa... more Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic patterns of neural activity in the isolated Drosophila central nervous system. Both imaging modalities were focused on individually identified bursicon neurons known to be involved in a fixed action pattern initiated by ecdysis-triggering hormone. We observed clear correspondence of OCT intensity, phase fluctuations, and activity-dependent calcium-induced fluorescence.
Optics in the Life Sciences, 2015
Current technologies for monitoring neural activity either use different variety of electrodes (e... more Current technologies for monitoring neural activity either use different variety of electrodes (electrical recording) or require contrast agents introduced exogenously or through genetic modification (optical imaging). Here we demonstrate an optical method for non-contact and contrast agent free detection of nerve activity using phase-resolved optical coherence tomography (pr-OCT). A common-path variation of the pr-OCT is recently implemented and the developed system demonstrated the capability to detect rapid transient structural changes that accompany neural spike propagation. No averaging over multiple trials was required, indicating its capability of single-shot detection of individual impulses from functionally stimulated Limulus optic nerve. The strength of this OCT-based optical electrode is that it is a contactless method and does not require any exogenous contrast agent. With further improvements in accuracy and sensitivity, this optical electrode will play a complementary ...