Mehboob-ur- Rahman - Academia.edu (original) (raw)

Papers by Mehboob-ur- Rahman

Pak. J. Bot, 2012

... Higher accumulation of TFAA helps plants to cope drought stress by executing different protec... more ... Higher accumulation of TFAA helps plants to cope drought stress by executing different protective/defensive functions like, osmotic adjustment, protection of cellular macromolecules, maintaining cellular pH, storage of ... Sarwar, MKS, I. Ullah, M. Rahman, MY Ashraf and Y. Zafar. ...

Bioinformatics in Agriculture

Bioinformatics - Updated Features and Applications, 2016

Plant Pathology, 2005

... Asad S, Haris WAA, Bashir A, Zafar Y, Malik KA, Malik MN, Lichtenstein CP, 2003. Transgenic .... more ... Asad S, Haris WAA, Bashir A, Zafar Y, Malik KA, Malik MN, Lichtenstein CP, 2003. Transgenic ... Crop Science 40, 98102. Mansoor S, Bashir A, Khan SH, Hussain M, Saeed M, Zafar Y, Markham PG, Malik KA, 1999a. Rapid ...

Pakistan Journal of Botany, 2022

Pakistan Journal of Botany, 2020

Cotton is the most vital source of fiber and food industry. Limited availability of water results... more Cotton is the most vital source of fiber and food industry. Limited availability of water results in the limited growth of plants. Drought responsive genes have been explored in several plants to be utilized for overcoming the dilemma of limited availability of water. Over expression of drought responsive genes to develop drought resistant cultivars is a promising strategy to combat drought stress. On exposure to drought, several genes linked to drought are activated and many of them are regulated by transcription factors. Recent developments in bioinformatics have made identification and In silico characterization of the genes possible. Di19 is the drought responsive transcription factor, which is involved in the drought tolerance pathways. This gene has been observed to interact with several other genes in the drought tolerance pathway in the plants. In the present study, this gene was amplified in Gossypium arboreum cultivar FDH-786 using primers based on GhDi19 gene sequence, fu...

Scientia Horticulturae, 2008

Knowledge about the extent of genetic diversity/relatedness in mango germplasm is vital for devel... more Knowledge about the extent of genetic diversity/relatedness in mango germplasm is vital for developing coherent strategies for future gains in productivity. The genetic diversity/relatedness among mango cultivars/genotypes developed in Pakistan has not been investigated ...

Electronic Journal of Biotechnology, 2011

Journal of Plant Registrations, 2007

All rights reserved. No part of this periodical may be reproduced or transmitted in any form or b... more All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.

Plant pathology, 2005

Asian J. Plant Sci, 2002

RAPD (Random Amplified Polymorphic DNA) technique was applied using bulked segregant analysis on ... more RAPD (Random Amplified Polymorphic DNA) technique was applied using bulked segregant analysis on the F 2 population of a cross between nectariless and nectariness genotypes to identify RAPD marker(s) for nectariless trait in upland cotton. Two bulked DNA samples were ...

Agronomy

Exploiting new genetic resources is an effective way to achieve sustainable wheat production. To ... more Exploiting new genetic resources is an effective way to achieve sustainable wheat production. To this end, we exposed wheat seeds of the “Punjab-11” cultivar to gamma rays. A total of 32 stable mutants (M7) were developed, followed by characterization by conducting multilocation trials over two seasons. Principal component analysis (PCA) showed that the first six components accounted for 90.28% of the total variation among the plant height, tillers per plant, 1000-kernel weight, grain yield, and quality traits. All mutants were grouped into three clusters based on high yield index values. The genotype by trait (GT) bi-plot revealed significant associations between yield and its components among the mutants. Positive correlations were estimated for tillers per plant, plant height, 1000-kernel weight, and grain yield; however, yield components showed negative associations with protein, moisture, and gluten contents. The mutant lines Pb-M-59 waxy, Pb-M-1272 waxy, Pb-M-2260, Pb-M-1027 w...

Nature Genetics

Allotetraploid cotton is an economically important natural-fiber-producing crop worldwide. After ... more Allotetraploid cotton is an economically important natural-fiber-producing crop worldwide. After polyploidization, Gossypium hirsutum L. evolved to produce a higher fiber yield and to better survive harsh environments than Gossypium barbadense, which produces superior-quality fibers. The global genetic and molecular bases for these interspecies divergences were unknown. Here we report high-quality de novo-assembled genomes for these two cultivated allotetraploid species with pronounced improvement in repetitive-DNA-enriched centromeric regions. Whole-genome comparative analyses revealed that speciesspecific alterations in gene expression, structural variations and expanded gene families were responsible for speciation and the evolutionary history of these species. These findings help to elucidate the evolution of cotton genomes and their domestication history. The information generated not only should enable breeders to improve fiber quality and resilience to ever-changing environmental conditions but also can be translated to other crops for better understanding of their domestication history and use in improvement.

International Journal of Agriculture and Biology, 2017

Genetic diversity was assessed among 38 chickpea (Cicer arietinum L.) genotypes on the basis of r... more Genetic diversity was assessed among 38 chickpea (Cicer arietinum L.) genotypes on the basis of random amplified polymorphic DNA (RAPD) in comparison with agro-morphological traits. Evaluation of agro-morphological traits revealed highly significant differences among genotypes. Days to 50% flowering ranged from 92-118, plant height 54.16-87 cm, number of fruit bearing branches 4-17.25, number of pods per plant 7.6-27.4, and grain yield per plant 3.5-9.8 g. Ascochyta blight (caused by Ascochyta rabiei) score of these genotypes was recorded on 1-9 rating scale that varied from 3-9. Cluster analysis showing relationship based on morphological traits (scale: Euclidean distance) placed 35 genotypes into five distinct groups, while three genotypes namely Noor-91, Local Mankera and BR4 did not include in any cluster. RAPD analysis showed that 35 RAPD primers amplified a total of 212 fragments out of which 45 were polymorphic. Polymorphic bands were generated by 21 primers whereas 14 primers were monomorphic. Genetic similarity matrix based on Nei and Li's index revealed similarity coefficients ranging from 92-97% indicating lower level of genetic polymorphism revealed by RAPD primers. Dandrogram constructed on the basis of these coefficients grouped all the genotypes into 2 major and 3 small clusters at 92% similarity level. Two decamers, OPC5 and OPC14 distinguished between three Desi and two Kabuli genotypes. This study showed that the level of genetic variability observed in chickpea for agro-morphological traits was not reflected in DNA polymorphism obtained by RAPD analysis.

Environmental Stresses in Soybean Production, 2016

The sustainable production of the soybean (Glycine max L. Merr.) is at stake due to increased sal... more The sustainable production of the soybean (Glycine max L. Merr.) is at stake due to increased salinization, frequent drought periods, flooding, unusual fluctuations in temperature, and rainfall pattern and frequency. The negative consequences of the changing climate are further complicating the situation. Conventionally, different traits such as root length, photosynthetic rate, seed size, etc. have been used as selection criteria for selecting resilient genotypes. Since 1995, DNA markers associated with different traits have been used for selecting the genotypes with excellent genetics; however, complex traits like tolerance to different abiotic stresses need further investigation to identify QTLs, which can be used confidently in initiating marker-assisted breeding in soybeans. A number of studies explored the various adaptation mechanisms at the molecular level, which help soybean plants to counter the abiotic stresses. For example, a number of transcription factors (TFs) including DREBs, ERFs, ZIP, WRKY, and MYB have been identified in soybeans. In another study, the role of the HD-Zip gene family in conferring drought and saline environments was described in soybeans. Similarly, a total of 61 HSP70 genes located unevenly on 17 different chromosomes were identified, which have a role in imparting tolerance to heat stress. Reports for transferring the genes and or TFs involved in conferring tolerance to abiotic stresses in soybeans are scanty. A P5CR gene, DREB1DTF, and the NTR1 gene were introduced in soybeans, which showed improved tolerance to drought. The expression of an Arabidopsis vacuolar Na+/H+antiporter gene (AtNHX1) in soybeans demonstrated improved tolerance to salt. Overexpression of a Solanumtorvum Δ1-pyrroline-5-carboxylate synthetase gene (StP5CS) in soybeans resulted in a higher level of salt tolerance. In the future, utilization of untapped genetic diversity available in the wild germplasm accessions, understanding genetic mechanisms using TILLING approach, identification of new DNA markers using next-gen sequencing tools, precise editing of the soybean genome using new editing tools like ZFNs and CRISPR-Cas9, and exploitation of the chloroplast-based transformation approach would be the ultimate choice for improving genetics of soybeans for mitigating the abiotic stresses.

TURKISH JOURNAL OF BOTANY, 2015

To the extent of our knowledge, applications of DNA markers in marker-assisted breeding of cotton... more To the extent of our knowledge, applications of DNA markers in marker-assisted breeding of cotton are handicapped due to low genetic diversity in cotton germplasm. Cotton leaf curl disease, a disease of viral origin, has substantially depressed cotton production in Pakistan, and this disease is also an emerging threat to the neighboring cotton-growing countries like China and India. The present study was designed to identify DNA markers, predominately simple sequence repeats (SSRs), associated with tolerance and/or resistance to the disease. Based upon 2 years of disease-screening field experiments, a total of 10 cotton genotypes (five highly tolerant, four highly susceptible, and one immune) of diverse origin were selected from the available cotton germplasm (~1200 accessions) of the National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. In total, 322 SSRs derived from bacterial artificial chromosome end sequences of Gossypium raimondii (one of the progenitor species of cultivated tetraploid cotton) were screened. Out of these, 65 primer pairs were found polymorphic, and the extent of genetic similarity was in the range of 81.7% to 98.7%. A similarity matrix was used for studying their phylogenetic relationship using unweighted pair-group method with arithmetic means (UPGMA) analysis. The dendrogram showed the grouping of the genotypes into two distinct clusters comprising tolerant and susceptible genotypes, respectively. Out of the polymorphic markers, two SSR markers, PR-91 and CM-43, that were amplified only in tolerant genotypes showed significant association with resistance to the disease. These preliminary results set the stage for initiating indepth marker-trait association studies, which will be instrumental for initiating marker-assisted breeding in cotton.