Melissa Kato - Academia.edu (original) (raw)
Papers by Melissa Kato
Clinical Oral Investigations, 2020
Objectives Evaluate the effect of dentifrices or gels containing MMP inhibitors on dentine loss i... more Objectives Evaluate the effect of dentifrices or gels containing MMP inhibitors on dentine loss in situ. Materials and methods Acrylic palatal appliances containing bovine dentine blocks were divided into two rows, corresponding to the groups erosion (ERO) and erosion associated with abrasion (ERO+ABR). For ERO, the appliances were immersed in a cola drink for 5 min, 4 times/day, while for ERO+ABR, the blocks were brushed for 15 sec with a dentifrice slurry after the second and third erosive challenges. Ten volunteers took part in study 1 (S1), where the dentifrices evaluated contained 1100 ppm fluoride as NaF, 0.61% green tea extract, or 0.012% chlorhexidine digluconate. Thirteen volunteers participated in study 2 (S2), in which the treatment was performed only once (1 min) with gels containing 400 μM EGCG (EGCG400), 0.012% chlorhexidine, 1 mM FeSO 4 , 1.23% F (NaF), placebo, or received no treatment. Dentine loss was analyzed by profilometry (μm). Results Regarding S1, ERO+ABR induced significantly higher dentine loss compared with ERO and all dentifrices tested led to significantly lower dentine loss when compared with placebo. For S2, regardless of the conditions or times of evaluation, gels containing EGCG, CHX, or FeSO 4 led to significantly less wear compared with the other groups. Conclusion Both dentifrices and gels containing MMP inhibitors significantly reduced dentine loss. Clinical relevance Dentifrices and gels containing MMP inhibitors are able to increase the protection against dentine wear, although gels have a better effect when compared with fluoride gel, lasting up to 10 days after a single application.
ain relief and reestablishment of normal jaw function are the main goals of conservative manageme... more ain relief and reestablishment of normal jaw function are the main goals of conservative management of Temporomandibular Disorders (TMD). Transcutaneous electrical nerve stimulation (TENS) and laser therapy are part of these modalities, although little is known about their real efficacy in controlled studies. This research compared these two treatments in a sample of 18 patients with chronic TMD of muscular origin, divided into two groups (LASER and TENS). Treatment consisted of ten sessions, in a period of 30 days. Active range of motion (AROM), visual analogue scale (VAS) of pain and muscle (masseter and anterior temporalis) palpation were used for follow-up analysis. Data were analyzed by Friedman test and ANOVA for repeated measurements. Results showed decrease in pain and increase in AROM for both groups (p<0.05), and improvement in muscle tenderness for the LASER group. Authors concluded that both therapies are effective as part of TMD management and a cumulative effect may...
Archives of Oral Biology
The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gel... more The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gels in inhibiting wear and degradation of demineralized organic matrix (DOM). 225 dentin specimens obtained from bovine incisors were randomly allocated into 5 groups (n=45): 10% Grape seed extract gel (GSE), 10% Cranberry extract gel (CE), 0.012% Chlorhexidine gel (CX), 1.23% NaF gel (F), and no active compound gel (P, placebo). Before the treatments, samples were demineralized by immersion in 0.87M citric acid, pH 2.3 (36h). Then, the studied gels were applied once over dentin for 1min. Next, the samples were immersed in artificial saliva containing collagenase obtained from Clostridium histolyticum for 5days. The response variable for dentin wear was depth of dentin loss measured by profilometry and for collagen degradation was hydroxyproline determination. Data were analyzed by ANOVA followed by Tukey&amp;amp;amp;#39;s test and Pearson Correlation Test (p&amp;amp;amp;lt;0.05). Grape seed extract significantly reduced dentin wear compared to the other groups (p&amp;amp;amp;lt;0.05). Cranberry extract and Chlorhexidine did not differ statistically and were able to reduce wear when compared to NaF and placebo treatments. The hydroxyproline analysis showed that there was no significant difference among groups for all treatments (p&amp;amp;amp;lt;0.05). Correlation analysis showed a significant correlation between the amount of degraded DOM evaluated by profilometry and the determination of hydroxyproline. Cranberry extract was able to reduce the dentin wear and collagen degradation, likely due to the proanthocyanidin content and its action. Therefore, Cranberry could be suggested as an interesting natural-based agent to prevent dentin erosion.
Journal of applied oral science : revista FOB, 2016
The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was sh... more The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was shown to prevent erosive wear. The aim of this study was to analyze the protective effect of toothpastes containing MMP inhibitors on dentine loss induced by erosion in vitro. Material and Methods Five groups each containing 12 specimens of human root dentine were prepared. The specimens were subjected to 1 min erosion by immersion in a cola drink, 4 times a day, for 5 d. Each day, after the first and last erosive challenges, the specimens were brushed for 15 s with a slurry of dentifrice and water (1:3) containing placebo, 1,100 ppm fluoride, 0.61% green tea extract, 0.12% chlorhexidine or 0.004% chlorhexidine (commercial toothpaste). Between the acid challenges, the specimens were stored in artificial saliva with remineralizing potential until the next treatment. Dentine loss was determined using profilometry. Data were analyzed using one-way ANOVA after log transform (p<0.05). Result...
Gels containing matrix metalloproteinases (MMPs) are able to reduce the progression of dentin ero... more Gels containing matrix metalloproteinases (MMPs) are able to reduce the progression of dentin erosion, but this was not tested for erosive challenges associated to abrasion neither for challenges conducted for more than 5 days in situ. Objectives: To evaluate the effect of gels containing different MMP inhibitors (epigallocatechin gallate-EGCG, chlorhexidine-CHX or ferrous sulphate-FeSO4) on the prevention of dentin erosion associated or not to abrasion in an in situ model conducted up to 10 days. Methods: Twelve adult volunteers wore palatal devices containing 12 bovine dentin blocks randomly allocated to 6 groups, according to the type of gels applied or not (not treated-NT). The gels had identical composition, except for the presence of EGCG (400M), CHX (0.012%), FeSO4 (1mM), fluoride (NaF-1.23%) or not (Placebo-P). Gels were applied or not on specimens once in a thin layer and removed after 1min. Erosion (5min, Coke, pH 2.6) was performed ex vivo, 4 times/day, for 10 days. Each ...
BMC Oral Health, 2014
Background: Dental erosion is caused by frequent exposure to acids without the involvement of mic... more Background: Dental erosion is caused by frequent exposure to acids without the involvement of microorganism. This study analyzed the effect of biguanides (polyhexamethylene biguanide-PHMB and chlorhexidine-CHX) on dentin erosion due to their possible influence on the enzymatic degradation of the demineralized organic matrix. Method: Sixty bovine dentin specimens were prepared. On both sides of their surface, nail varnish was applied to maintain the reference surfaces for the determination of dentin loss. Samples were cyclically de-and remineralized for 6 days. Demineralization was performed with a 0.87 M citric acid solution (6×5 min daily). Thereafter, samples were treated with distilled water (negative control), 0.12% CHX (positive control), 0.07% PHMB, Sanifill Perio Premium™ (0.07% PHMB plus 0.05% NaF), or F solution (0.05% NaF) for 1 min and then subjected to enzymatic challenge for 10 min using a bacterial collagenase (Clostridium hystoliticum, 100 μg/ml). Dentin loss was assessed using profilometry (μm) daily. Data were analyzed using 2-way repeated measures-ANOVA and Bonferroni's test (p < 0.05). Results: Dentin loss progressed significantly for all groups during the 6 days. After the 3 rd day, Sanifill Premium™, CHX, and PHMB significantly reduced dentin erosion compared to control. On the 6 th day, the lowest mean (±SD) dentin loss was observed for Sanifill Perio Premium™ (94.4 ± 3.9 μm). PHMB and CHX led to intermediate dentin loss (129.9 ± 41.2 and 135.3 ± 33.5 μm, respectively) that was significantly lower than those found for negative control (168.2 ± 6.2 μm). F (157.4 ± 6.1 μm) did not significantly differ from negative control. Conclusions: Sanifill Perio Premium™ mouthwash has a good potential to reduce dentin loss, which might be associated with the presence of PHMB.
Journal of Applied Oral Science, 2012
D ental erosion is a multifactorial condition. The consideration of chemical, biological and beha... more D ental erosion is a multifactorial condition. The consideration of chemical, biological and behavioral factors is fundamental for its prevention and therapy. Among the biological factors, saliva is one of the most important parameters in the protection against erosive wear. Objective: This review discusses the role of salivary factors on the development of dental erosion. Material and Methods: A search was undertaken on MeDLINe website for papers from 1969 to 2010. The keywords used in the research were "saliva", "acquired pellicle", "salivary flow", "salivary buffering capacity" and "dental erosion". Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. Results: Several characteristics and properties of saliva play an important role in dental erosion. Salivary clearance gradually eliminates the acids through swallowing and saliva presents buffering capacity causing neutralization and buffering of dietary acids. Salivary flow allows dilution of the acids. In addition, saliva is supersaturated with respect to tooth mineral, providing calcium, phosphate and fluoride necessary for remineralization after an erosive challenge. Furthermore, many proteins present in saliva and acquired pellicle play an important role in dental erosion. Conclusions: Saliva is the most important biological factor affecting the progression of dental erosion. Knowledge of its components and properties involved in this protective role can drive the development of preventive measures targeting to enhance its known beneficial effects.
Journal of Applied Oral Science, 2012
This in vitro study evaluated the effect of calcium glycerophosphate (CaGP) supplemented to soft ... more This in vitro study evaluated the effect of calcium glycerophosphate (CaGP) supplemented to soft drinks on bovine enamel erosion. Material and methods: Four pHcycles were performed, alternating demineralization by the beverage and remineralization LQ DUWL¿FLDO VDOLYD 5HVXOWV 0HDQ ZHDU 6' P ZDV and 5.21±1.08 for Coca-Cola™ without CaGP or containing CaGP at 0.1, 1.0 or 2.0 mM, respectively, while no wear was detected for CaGP at 5.0 and 10.0 mM. Corresponding ¿JXUHV IRU 6SULWH =HUR ZLWKRXW &D*3 RU FRQWDLQLQJ &D*3 DW RU mM were 8.04±1.30, 7.84±0.71, 7.47±0.80, 4.96±0.81, 3.99±0.10 and 1.87±0.12, respectively. Conclusion: Supplementation of both beverages with CaGP seems to be an alternative to reduce their erosive potential.
Journal of Applied Oral Science, 2012
Australian Dental Journal, 2011
Clinical Oral Investigations, 2020
Objectives Evaluate the effect of dentifrices or gels containing MMP inhibitors on dentine loss i... more Objectives Evaluate the effect of dentifrices or gels containing MMP inhibitors on dentine loss in situ. Materials and methods Acrylic palatal appliances containing bovine dentine blocks were divided into two rows, corresponding to the groups erosion (ERO) and erosion associated with abrasion (ERO+ABR). For ERO, the appliances were immersed in a cola drink for 5 min, 4 times/day, while for ERO+ABR, the blocks were brushed for 15 sec with a dentifrice slurry after the second and third erosive challenges. Ten volunteers took part in study 1 (S1), where the dentifrices evaluated contained 1100 ppm fluoride as NaF, 0.61% green tea extract, or 0.012% chlorhexidine digluconate. Thirteen volunteers participated in study 2 (S2), in which the treatment was performed only once (1 min) with gels containing 400 μM EGCG (EGCG400), 0.012% chlorhexidine, 1 mM FeSO 4 , 1.23% F (NaF), placebo, or received no treatment. Dentine loss was analyzed by profilometry (μm). Results Regarding S1, ERO+ABR induced significantly higher dentine loss compared with ERO and all dentifrices tested led to significantly lower dentine loss when compared with placebo. For S2, regardless of the conditions or times of evaluation, gels containing EGCG, CHX, or FeSO 4 led to significantly less wear compared with the other groups. Conclusion Both dentifrices and gels containing MMP inhibitors significantly reduced dentine loss. Clinical relevance Dentifrices and gels containing MMP inhibitors are able to increase the protection against dentine wear, although gels have a better effect when compared with fluoride gel, lasting up to 10 days after a single application.
ain relief and reestablishment of normal jaw function are the main goals of conservative manageme... more ain relief and reestablishment of normal jaw function are the main goals of conservative management of Temporomandibular Disorders (TMD). Transcutaneous electrical nerve stimulation (TENS) and laser therapy are part of these modalities, although little is known about their real efficacy in controlled studies. This research compared these two treatments in a sample of 18 patients with chronic TMD of muscular origin, divided into two groups (LASER and TENS). Treatment consisted of ten sessions, in a period of 30 days. Active range of motion (AROM), visual analogue scale (VAS) of pain and muscle (masseter and anterior temporalis) palpation were used for follow-up analysis. Data were analyzed by Friedman test and ANOVA for repeated measurements. Results showed decrease in pain and increase in AROM for both groups (p<0.05), and improvement in muscle tenderness for the LASER group. Authors concluded that both therapies are effective as part of TMD management and a cumulative effect may...
Archives of Oral Biology
The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gel... more The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gels in inhibiting wear and degradation of demineralized organic matrix (DOM). 225 dentin specimens obtained from bovine incisors were randomly allocated into 5 groups (n=45): 10% Grape seed extract gel (GSE), 10% Cranberry extract gel (CE), 0.012% Chlorhexidine gel (CX), 1.23% NaF gel (F), and no active compound gel (P, placebo). Before the treatments, samples were demineralized by immersion in 0.87M citric acid, pH 2.3 (36h). Then, the studied gels were applied once over dentin for 1min. Next, the samples were immersed in artificial saliva containing collagenase obtained from Clostridium histolyticum for 5days. The response variable for dentin wear was depth of dentin loss measured by profilometry and for collagen degradation was hydroxyproline determination. Data were analyzed by ANOVA followed by Tukey&amp;amp;amp;#39;s test and Pearson Correlation Test (p&amp;amp;amp;lt;0.05). Grape seed extract significantly reduced dentin wear compared to the other groups (p&amp;amp;amp;lt;0.05). Cranberry extract and Chlorhexidine did not differ statistically and were able to reduce wear when compared to NaF and placebo treatments. The hydroxyproline analysis showed that there was no significant difference among groups for all treatments (p&amp;amp;amp;lt;0.05). Correlation analysis showed a significant correlation between the amount of degraded DOM evaluated by profilometry and the determination of hydroxyproline. Cranberry extract was able to reduce the dentin wear and collagen degradation, likely due to the proanthocyanidin content and its action. Therefore, Cranberry could be suggested as an interesting natural-based agent to prevent dentin erosion.
Journal of applied oral science : revista FOB, 2016
The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was sh... more The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was shown to prevent erosive wear. The aim of this study was to analyze the protective effect of toothpastes containing MMP inhibitors on dentine loss induced by erosion in vitro. Material and Methods Five groups each containing 12 specimens of human root dentine were prepared. The specimens were subjected to 1 min erosion by immersion in a cola drink, 4 times a day, for 5 d. Each day, after the first and last erosive challenges, the specimens were brushed for 15 s with a slurry of dentifrice and water (1:3) containing placebo, 1,100 ppm fluoride, 0.61% green tea extract, 0.12% chlorhexidine or 0.004% chlorhexidine (commercial toothpaste). Between the acid challenges, the specimens were stored in artificial saliva with remineralizing potential until the next treatment. Dentine loss was determined using profilometry. Data were analyzed using one-way ANOVA after log transform (p<0.05). Result...
Gels containing matrix metalloproteinases (MMPs) are able to reduce the progression of dentin ero... more Gels containing matrix metalloproteinases (MMPs) are able to reduce the progression of dentin erosion, but this was not tested for erosive challenges associated to abrasion neither for challenges conducted for more than 5 days in situ. Objectives: To evaluate the effect of gels containing different MMP inhibitors (epigallocatechin gallate-EGCG, chlorhexidine-CHX or ferrous sulphate-FeSO4) on the prevention of dentin erosion associated or not to abrasion in an in situ model conducted up to 10 days. Methods: Twelve adult volunteers wore palatal devices containing 12 bovine dentin blocks randomly allocated to 6 groups, according to the type of gels applied or not (not treated-NT). The gels had identical composition, except for the presence of EGCG (400M), CHX (0.012%), FeSO4 (1mM), fluoride (NaF-1.23%) or not (Placebo-P). Gels were applied or not on specimens once in a thin layer and removed after 1min. Erosion (5min, Coke, pH 2.6) was performed ex vivo, 4 times/day, for 10 days. Each ...
BMC Oral Health, 2014
Background: Dental erosion is caused by frequent exposure to acids without the involvement of mic... more Background: Dental erosion is caused by frequent exposure to acids without the involvement of microorganism. This study analyzed the effect of biguanides (polyhexamethylene biguanide-PHMB and chlorhexidine-CHX) on dentin erosion due to their possible influence on the enzymatic degradation of the demineralized organic matrix. Method: Sixty bovine dentin specimens were prepared. On both sides of their surface, nail varnish was applied to maintain the reference surfaces for the determination of dentin loss. Samples were cyclically de-and remineralized for 6 days. Demineralization was performed with a 0.87 M citric acid solution (6×5 min daily). Thereafter, samples were treated with distilled water (negative control), 0.12% CHX (positive control), 0.07% PHMB, Sanifill Perio Premium™ (0.07% PHMB plus 0.05% NaF), or F solution (0.05% NaF) for 1 min and then subjected to enzymatic challenge for 10 min using a bacterial collagenase (Clostridium hystoliticum, 100 μg/ml). Dentin loss was assessed using profilometry (μm) daily. Data were analyzed using 2-way repeated measures-ANOVA and Bonferroni's test (p < 0.05). Results: Dentin loss progressed significantly for all groups during the 6 days. After the 3 rd day, Sanifill Premium™, CHX, and PHMB significantly reduced dentin erosion compared to control. On the 6 th day, the lowest mean (±SD) dentin loss was observed for Sanifill Perio Premium™ (94.4 ± 3.9 μm). PHMB and CHX led to intermediate dentin loss (129.9 ± 41.2 and 135.3 ± 33.5 μm, respectively) that was significantly lower than those found for negative control (168.2 ± 6.2 μm). F (157.4 ± 6.1 μm) did not significantly differ from negative control. Conclusions: Sanifill Perio Premium™ mouthwash has a good potential to reduce dentin loss, which might be associated with the presence of PHMB.
Journal of Applied Oral Science, 2012
D ental erosion is a multifactorial condition. The consideration of chemical, biological and beha... more D ental erosion is a multifactorial condition. The consideration of chemical, biological and behavioral factors is fundamental for its prevention and therapy. Among the biological factors, saliva is one of the most important parameters in the protection against erosive wear. Objective: This review discusses the role of salivary factors on the development of dental erosion. Material and Methods: A search was undertaken on MeDLINe website for papers from 1969 to 2010. The keywords used in the research were "saliva", "acquired pellicle", "salivary flow", "salivary buffering capacity" and "dental erosion". Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. Results: Several characteristics and properties of saliva play an important role in dental erosion. Salivary clearance gradually eliminates the acids through swallowing and saliva presents buffering capacity causing neutralization and buffering of dietary acids. Salivary flow allows dilution of the acids. In addition, saliva is supersaturated with respect to tooth mineral, providing calcium, phosphate and fluoride necessary for remineralization after an erosive challenge. Furthermore, many proteins present in saliva and acquired pellicle play an important role in dental erosion. Conclusions: Saliva is the most important biological factor affecting the progression of dental erosion. Knowledge of its components and properties involved in this protective role can drive the development of preventive measures targeting to enhance its known beneficial effects.
Journal of Applied Oral Science, 2012
This in vitro study evaluated the effect of calcium glycerophosphate (CaGP) supplemented to soft ... more This in vitro study evaluated the effect of calcium glycerophosphate (CaGP) supplemented to soft drinks on bovine enamel erosion. Material and methods: Four pHcycles were performed, alternating demineralization by the beverage and remineralization LQ DUWL¿FLDO VDOLYD 5HVXOWV 0HDQ ZHDU 6' P ZDV and 5.21±1.08 for Coca-Cola™ without CaGP or containing CaGP at 0.1, 1.0 or 2.0 mM, respectively, while no wear was detected for CaGP at 5.0 and 10.0 mM. Corresponding ¿JXUHV IRU 6SULWH =HUR ZLWKRXW &D*3 RU FRQWDLQLQJ &D*3 DW RU mM were 8.04±1.30, 7.84±0.71, 7.47±0.80, 4.96±0.81, 3.99±0.10 and 1.87±0.12, respectively. Conclusion: Supplementation of both beverages with CaGP seems to be an alternative to reduce their erosive potential.
Journal of Applied Oral Science, 2012
Australian Dental Journal, 2011