Merve Becit - Academia.edu (original) (raw)
Papers by Merve Becit
Health Sciences Quarterly
CYP1B1 is a P450 enzyme involved in activating pro-carcinogens to carcinogens as well as estrogen... more CYP1B1 is a P450 enzyme involved in activating pro-carcinogens to carcinogens as well as estrogen metabolism. In order to examine the effect of CYP1B1 on cancer metabolism, it should be compared with healthy individuals and whether the polymorphism between healthy individuals and sick individuals is significant. This study aims to screen the CYP1B1*3 and CYP1B1*4 polymorphisms of a group of individuals who have not been diagnosed with cancer to examine the genetic differences of metabolic enzymes in the Turkish population.This study is a cross-sectional type descriptive study.The study included 295 patients without a cancer diagnosis. The research sample includes patients who applied to Ankara University Medical Faculty Hospital and Afyonkarahisar Health Sciences University Research and Application Hospital. The individuals signed voluntary consent forms before participation, and 3 ml blood samples were taken from each. DNA samples were obtained using a DNA isolation kit, and then p...
Workers working in marble processing plants are exposed to high levels of respirable marble dust ... more Workers working in marble processing plants are exposed to high levels of respirable marble dust (<10 μm) in processes such as cutting, polishing, shaping. Marble dust is mainly composed of calcium carbonate and contains crystalline silica in different proportions. It is thought that both particulate dust and crystalline silica may be responsible for the adverse health effects of marble dust. There are a limited number of studies evaluating the genotoxicity and oxidative stress parameters of workers occupationally exposed to marble dust. In this study, among the workers working in the marble processing plants in Iscehisar (Afyonkarahisar) region; it is aimed to clarify how marble dust affects oxidative stress, genotoxicity and immunotoxicity mechanisms. Oxidative stress and immune system parameters were determined spectrophotometrically using commercial kits. Genotoxicity was evaluated by single cell gel electrophoresis (comet) and micronucleus methods in lymphocytes and buccal e...
Pinus pinaster’den elde edilen piknogenol ve Curcuma longa L.’dan elde edilen kurkumin polifenoli... more Pinus pinaster’den elde edilen piknogenol ve Curcuma longa L.’dan elde edilen kurkumin polifenolik bilesikler olup, yuksek antioksidan ozellikleri nedeniyle geleneksel olarak bircok hastalikta yaygin kullanilmaktadir. Sisplatin, testis, over, mesane, prostat, serviks, akciger kanseri gibi solid tumorlerin tedavisinde siklikla kullanilan antineoplastik bir ajandir. Kanser tedavisinde kemoterapotik ilaclarla birlikte cesitli bitkisel kaynakli fenolik bilesikler kullanilarak antikanser etkinin arttirilmasi, sitotoksisitenin azaltilmasi amaclanmakta ve arastirilmaktadir. Piknogenol ve kurkuminin antikanser ilaclarla etkilesmeleri uzerinde yapilan calismalar yetersiz oldugundan, bu tez calismasinda bu fenolik bilesiklerin Cin hamster akciger fibroblast (V79), insan karaciger kanser (HepG2) ve insan serviks kanser (HeLa) hucrelerinde MTT yontemi ile sitotoksisitesi belirlenerek sisplatin ile kombinasyonlarinda hucre canliliginin degerlendirilmesi ve antikanser etkilerine aciklik getirilme...
Toxicology Letters, 2016
Kanser tedavisinde antikanser etkiyi artırmak ve sitotoksisiteyi azaltmak amacıyla kemoterapötik ... more Kanser tedavisinde antikanser etkiyi artırmak ve sitotoksisiteyi azaltmak amacıyla kemoterapötik ilaçlar ile birlikte çeşitli bitkisel kökenli fenolik bileşiklerin kullanımı hedeflenmektedir. Bir fenolik bileşik olan Piknogenol ® (PYC), birçok çalışmanın konusu olmaktadır. PYC'nin sisplatin ile etkileşme mekanizması tam olarak aydınlatılamadığı için insan serviks kanser hücrelerinde (HeLa) sisplatin sitotoksisitesi üzerine PYC'nin etkilerini belirlemeyi ve PYC'nin sitotoksik olmayan dozlarında PYC'nin genotoksisitesini değerlendirilmeyi hedefledik. Gereç ve Yöntemler: HeLa hücrelerinde, 24 ve 48 saatlik maruziyetlerde, PYC varlığında ve yokluğunda sisplatinin sitotoksisitesi 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) yöntemi ile ölçüldü. Oksidatif DNA hasarına karşı PYC'nin etkisi Comet yöntemi ile değerlendirildi. Bulgular: Sisplatinin IC 50 değeri 24 saat ve 48 saat için sırasıyla 22,4 µM ve 12,3 µM idi. PYC'nin IC 50 değerleri 24 saat ve 48 saat için sırasıyla 261 µM ve 213 µM idi. Yirmi dört saatlik maruziyet için, PYC'nin, seçilen konsantrasyonlarda (15,6-500 µM) sisplatinin IC 50 değerini önemli ölçüde azalttı. Kırk sekiz saat maruziyet için, PYC sisplatinin sitotoksisitesini 15,6-125 µM arasındaki konsantrasyonlarda değiştirmedi, ancak 250 µM ve 500 µM konsantrasyonlarda önemli ölçüde azalttı. PYC tek başına 10 µM ve 25 µM konsantrasyonlarında DNA hasarına neden olmadı, ancak daha yüksek konsantrasyonlarında (50-100 µM) DNA hasarını önemli ölçüde indükledi. Ayrıca, çalışılan tüm konsantrasyonlarında (10-100 µM) 50 µM H 2 O 2 tarafından indüklenen DNA hasarını önemli ölçüde azalttı. Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol ® (PYC), a phenolic compound, has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin need to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa) and to evaluate the genotoxicity of PYC. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC 50 values of cisplatin were 22.4 µM and 12.3 µM for 24 h and 48 h, respectively. The IC 50 values of PYC were 261 µM and 213 µM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly reduced the IC 50 value of cisplatin at the selected concentrations (15.6-500 µM). For 48 h exposure, PYC did not change the cytotoxicity of cisplatin at concentrations between 15.6 and 125 µM, but significantly reduced it at concentrations of 250 µM and 500 µM. PYC alone did not induce DNA damage at concentrations of 10 µM or 25 µM; however, it significantly induced DNA damage at higher concentrations (50-100 µM). It also significantly reduced H 2 O 2-induced DNA damage at all concentrations studied (10-100 µM). Conclusion: Our results suggest that PYC may increase the cisplatin cytotoxicity in HeLa cells at nongenotoxic doses. The results might contribute to the anticancer effect of cisplatin with PYC in cervical carcinoma, but in order to confirm this result further in vitro studies with cancer cell lines and in vivo studies are needed.
Environmental research, 2021
Workers in marble processing plants are at high risk of exposure to high levels of marble dust co... more Workers in marble processing plants are at high risk of exposure to high levels of marble dust containing silica, but there are limited studies evaluating the genotoxicity and oxidative stress parameters of workers occupationally exposed to marble dust. In this study, we aimed to clarify how marble dust affects genotoxicity and immunotoxicity mechanisms alongside oxidative stress in the workers in the marble processing plants of Iscehisar, Turkey. The oxidative stress and immune system parameters were determined spectrophotometrically using commercial kits. Genotoxicity was evaluated by Comet and micronucleus (MN) assays in the lymphocytes and buccal cells, respectively. The enzyme activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and the levels of glutathione, and Clara cell secretory protein CC16 in workers (n=48) were significantly lower than in controls (n=41), whereas the levels of malondialdehyde, 8-oxo-7,8-dihydro-2'-deoxyguanosi...
Objectives: In the treatment of cancer, it is intended to increase anticancer effect and decrease... more Objectives: In the treatment of cancer, it is intended to increase anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic compound has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin needs to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa), as well as the genotoxicity of PYC was also evaluated. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured using 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC50 values of cisplatin were 22.4 μM and 12.3 μM for 24 h and 48 h, respectively. The IC50 values of pycnogenol were 261 μM and 213 μM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly ...
Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decr... more Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic compound, has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin need to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa) and to evaluate the genotoxicity of PYC. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC50 values of cisplatin were 22.4 μM and 12.3 μM for 24 h and 48 h, respectively. The IC50 values of PYC were 261 μM and 213 μM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly reduced the IC50 val...
Turkiye Klinikleri Journal of Pharmacy Sciences
Turkiye Klinikleri Journal of Pharmacy Sciences, 2017
In this article, genotoxic and mutagenic effects of mycotoxins that are produced by various fungu... more In this article, genotoxic and mutagenic effects of mycotoxins that are produced by various fungus species have been reviewed. A total of 259 mycotoxins were found in the literature. Genotoxic effects of 109 of these were investigated. Among the studied mycotoxins, only actinomycin D, aflatoxin, alternariol, chrysazin (dantron), citrinin, fumonisin, mytomycin C, nivalenol, ochratoxin A, patulin, sterigmatocystin, versicolorin A and B, vomitoxin, and zearalenone have sufficient number of studies that present or prove their genotoxic effects. Additional studies are required in order to determine whether other mycotoxins have any genotoxic effects. The current study provides valuable information regarding studied mycotoxins. Therefore, it may lead researchers for designing future mycotoxin-related studies that have never been studied.
İstanbul Journal of Pharmacy
Health Sciences Quarterly
CYP1B1 is a P450 enzyme involved in activating pro-carcinogens to carcinogens as well as estrogen... more CYP1B1 is a P450 enzyme involved in activating pro-carcinogens to carcinogens as well as estrogen metabolism. In order to examine the effect of CYP1B1 on cancer metabolism, it should be compared with healthy individuals and whether the polymorphism between healthy individuals and sick individuals is significant. This study aims to screen the CYP1B1*3 and CYP1B1*4 polymorphisms of a group of individuals who have not been diagnosed with cancer to examine the genetic differences of metabolic enzymes in the Turkish population.This study is a cross-sectional type descriptive study.The study included 295 patients without a cancer diagnosis. The research sample includes patients who applied to Ankara University Medical Faculty Hospital and Afyonkarahisar Health Sciences University Research and Application Hospital. The individuals signed voluntary consent forms before participation, and 3 ml blood samples were taken from each. DNA samples were obtained using a DNA isolation kit, and then p...
Workers working in marble processing plants are exposed to high levels of respirable marble dust ... more Workers working in marble processing plants are exposed to high levels of respirable marble dust (<10 μm) in processes such as cutting, polishing, shaping. Marble dust is mainly composed of calcium carbonate and contains crystalline silica in different proportions. It is thought that both particulate dust and crystalline silica may be responsible for the adverse health effects of marble dust. There are a limited number of studies evaluating the genotoxicity and oxidative stress parameters of workers occupationally exposed to marble dust. In this study, among the workers working in the marble processing plants in Iscehisar (Afyonkarahisar) region; it is aimed to clarify how marble dust affects oxidative stress, genotoxicity and immunotoxicity mechanisms. Oxidative stress and immune system parameters were determined spectrophotometrically using commercial kits. Genotoxicity was evaluated by single cell gel electrophoresis (comet) and micronucleus methods in lymphocytes and buccal e...
Pinus pinaster’den elde edilen piknogenol ve Curcuma longa L.’dan elde edilen kurkumin polifenoli... more Pinus pinaster’den elde edilen piknogenol ve Curcuma longa L.’dan elde edilen kurkumin polifenolik bilesikler olup, yuksek antioksidan ozellikleri nedeniyle geleneksel olarak bircok hastalikta yaygin kullanilmaktadir. Sisplatin, testis, over, mesane, prostat, serviks, akciger kanseri gibi solid tumorlerin tedavisinde siklikla kullanilan antineoplastik bir ajandir. Kanser tedavisinde kemoterapotik ilaclarla birlikte cesitli bitkisel kaynakli fenolik bilesikler kullanilarak antikanser etkinin arttirilmasi, sitotoksisitenin azaltilmasi amaclanmakta ve arastirilmaktadir. Piknogenol ve kurkuminin antikanser ilaclarla etkilesmeleri uzerinde yapilan calismalar yetersiz oldugundan, bu tez calismasinda bu fenolik bilesiklerin Cin hamster akciger fibroblast (V79), insan karaciger kanser (HepG2) ve insan serviks kanser (HeLa) hucrelerinde MTT yontemi ile sitotoksisitesi belirlenerek sisplatin ile kombinasyonlarinda hucre canliliginin degerlendirilmesi ve antikanser etkilerine aciklik getirilme...
Toxicology Letters, 2016
Kanser tedavisinde antikanser etkiyi artırmak ve sitotoksisiteyi azaltmak amacıyla kemoterapötik ... more Kanser tedavisinde antikanser etkiyi artırmak ve sitotoksisiteyi azaltmak amacıyla kemoterapötik ilaçlar ile birlikte çeşitli bitkisel kökenli fenolik bileşiklerin kullanımı hedeflenmektedir. Bir fenolik bileşik olan Piknogenol ® (PYC), birçok çalışmanın konusu olmaktadır. PYC'nin sisplatin ile etkileşme mekanizması tam olarak aydınlatılamadığı için insan serviks kanser hücrelerinde (HeLa) sisplatin sitotoksisitesi üzerine PYC'nin etkilerini belirlemeyi ve PYC'nin sitotoksik olmayan dozlarında PYC'nin genotoksisitesini değerlendirilmeyi hedefledik. Gereç ve Yöntemler: HeLa hücrelerinde, 24 ve 48 saatlik maruziyetlerde, PYC varlığında ve yokluğunda sisplatinin sitotoksisitesi 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) yöntemi ile ölçüldü. Oksidatif DNA hasarına karşı PYC'nin etkisi Comet yöntemi ile değerlendirildi. Bulgular: Sisplatinin IC 50 değeri 24 saat ve 48 saat için sırasıyla 22,4 µM ve 12,3 µM idi. PYC'nin IC 50 değerleri 24 saat ve 48 saat için sırasıyla 261 µM ve 213 µM idi. Yirmi dört saatlik maruziyet için, PYC'nin, seçilen konsantrasyonlarda (15,6-500 µM) sisplatinin IC 50 değerini önemli ölçüde azalttı. Kırk sekiz saat maruziyet için, PYC sisplatinin sitotoksisitesini 15,6-125 µM arasındaki konsantrasyonlarda değiştirmedi, ancak 250 µM ve 500 µM konsantrasyonlarda önemli ölçüde azalttı. PYC tek başına 10 µM ve 25 µM konsantrasyonlarında DNA hasarına neden olmadı, ancak daha yüksek konsantrasyonlarında (50-100 µM) DNA hasarını önemli ölçüde indükledi. Ayrıca, çalışılan tüm konsantrasyonlarında (10-100 µM) 50 µM H 2 O 2 tarafından indüklenen DNA hasarını önemli ölçüde azalttı. Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol ® (PYC), a phenolic compound, has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin need to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa) and to evaluate the genotoxicity of PYC. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC 50 values of cisplatin were 22.4 µM and 12.3 µM for 24 h and 48 h, respectively. The IC 50 values of PYC were 261 µM and 213 µM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly reduced the IC 50 value of cisplatin at the selected concentrations (15.6-500 µM). For 48 h exposure, PYC did not change the cytotoxicity of cisplatin at concentrations between 15.6 and 125 µM, but significantly reduced it at concentrations of 250 µM and 500 µM. PYC alone did not induce DNA damage at concentrations of 10 µM or 25 µM; however, it significantly induced DNA damage at higher concentrations (50-100 µM). It also significantly reduced H 2 O 2-induced DNA damage at all concentrations studied (10-100 µM). Conclusion: Our results suggest that PYC may increase the cisplatin cytotoxicity in HeLa cells at nongenotoxic doses. The results might contribute to the anticancer effect of cisplatin with PYC in cervical carcinoma, but in order to confirm this result further in vitro studies with cancer cell lines and in vivo studies are needed.
Environmental research, 2021
Workers in marble processing plants are at high risk of exposure to high levels of marble dust co... more Workers in marble processing plants are at high risk of exposure to high levels of marble dust containing silica, but there are limited studies evaluating the genotoxicity and oxidative stress parameters of workers occupationally exposed to marble dust. In this study, we aimed to clarify how marble dust affects genotoxicity and immunotoxicity mechanisms alongside oxidative stress in the workers in the marble processing plants of Iscehisar, Turkey. The oxidative stress and immune system parameters were determined spectrophotometrically using commercial kits. Genotoxicity was evaluated by Comet and micronucleus (MN) assays in the lymphocytes and buccal cells, respectively. The enzyme activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and the levels of glutathione, and Clara cell secretory protein CC16 in workers (n=48) were significantly lower than in controls (n=41), whereas the levels of malondialdehyde, 8-oxo-7,8-dihydro-2'-deoxyguanosi...
Objectives: In the treatment of cancer, it is intended to increase anticancer effect and decrease... more Objectives: In the treatment of cancer, it is intended to increase anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic compound has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin needs to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa), as well as the genotoxicity of PYC was also evaluated. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured using 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC50 values of cisplatin were 22.4 μM and 12.3 μM for 24 h and 48 h, respectively. The IC50 values of pycnogenol were 261 μM and 213 μM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly ...
Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decr... more Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic compound, has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin need to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa) and to evaluate the genotoxicity of PYC. Materials and Methods: The cytotoxicity of cisplatin and PYC was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay. Results: The IC50 values of cisplatin were 22.4 μM and 12.3 μM for 24 h and 48 h, respectively. The IC50 values of PYC were 261 μM and 213 μM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly reduced the IC50 val...
Turkiye Klinikleri Journal of Pharmacy Sciences
Turkiye Klinikleri Journal of Pharmacy Sciences, 2017
In this article, genotoxic and mutagenic effects of mycotoxins that are produced by various fungu... more In this article, genotoxic and mutagenic effects of mycotoxins that are produced by various fungus species have been reviewed. A total of 259 mycotoxins were found in the literature. Genotoxic effects of 109 of these were investigated. Among the studied mycotoxins, only actinomycin D, aflatoxin, alternariol, chrysazin (dantron), citrinin, fumonisin, mytomycin C, nivalenol, ochratoxin A, patulin, sterigmatocystin, versicolorin A and B, vomitoxin, and zearalenone have sufficient number of studies that present or prove their genotoxic effects. Additional studies are required in order to determine whether other mycotoxins have any genotoxic effects. The current study provides valuable information regarding studied mycotoxins. Therefore, it may lead researchers for designing future mycotoxin-related studies that have never been studied.
İstanbul Journal of Pharmacy