Michèle Salmain - Academia.edu (original) (raw)

Papers by Michèle Salmain

The Journal of Physical Chemistry B, 2008

CITATIONS 48 READS 75 6 authors, including: Some of the authors of this publication are also work... more CITATIONS 48 READS 75 6 authors, including: Some of the authors of this publication are also working on these related projects: portable high sensitive optical sensor View project Milking ability and milking of sicilosarda dairy ewes in Tunisia View project Souhir Boujday Pierre and Marie Curie University -Paris 6

The Journal of Physical Chemistry B, 2009

In the aim of protecting stainless steel surfaces against protein and/or bacterial adhesion, thin... more In the aim of protecting stainless steel surfaces against protein and/or bacterial adhesion, thin films including the glycosidase hen egg white lysozyme (HEWL) and/or the synthetic polymer poly(ethylene glycol) (PEG) were covalently coated onto flat substrates by wet chemical processes. Chemical grafting of both species was carried out by covalent binding to surfaces pretreated by the polyamine poly(ethylene imine) (PEI). Surfaces were characterized at each step of functionalization by means of reflection-absorption infrared spectroscopy by modulation of polarization (PM-RAIRS) and X-ray photoelectron spectroscopy (XPS) to determine the atomic and molecular composition of the interfaces, respectively. Then, the ability of the so-modified surfaces to prevent protein adsorption and bacterial adhesion together with their biocide properties were demonstrated by three local tests employing bovine serum albumin (BSA), and the bacteria Listeria ivanovii and Micrococcus luteus. A new test was implemented to assess the local enzymatic properties of HEWL. Cografting of PEG and HEWL resulted in a surface with both antiadhesion and antibacterial properties.

Tetrahedron Letters, 2008

The cationic (η6-arene)ruthenium complexes 6–9 containing a chloroacetamide or a maleimide functi... more The cationic (η6-arene)ruthenium complexes 6–9 containing a chloroacetamide or a maleimide functional group on the arene ligand were synthesized and successfully used to introduce ruthenium(II) species to the active site of the cysteine endoproteinase papain in a site-directed and covalent fashion as shown by enzymatic and ESI-MS studies.

Talanta, 2009

This paper describes the elaboration of a model immunosensor monitored by polarization modulation... more This paper describes the elaboration of a model immunosensor monitored by polarization modulation reflection absorption infrared spectroscopy (PM-RAIRS) and quartz crystal microbalance (QCM-D), as well as its reactivity using PM-RAIRS as transduction technique. To prove its relevance, this immunosensor was applied to the detection of benzo[a]pyrene (BaP), a carcinogenic polycylic aromatic hydrocarbon. Very few immunoassays, and even fewer immunosensors, have been described for the assay of BaP, making it an interesting target for analytical device development. The PM-RAIRS immunosensor was constructed on planar gold-coated sensors functionalized with cystamine then glutaraldehyde. Antibodies were immobilized through their affinity to protein G, covalently coupled to the aldehyde layer. In a first stage, a model mouse IgG was utilized to optimize the elaboration parameters; then, a monoclonal anti-PAH antibody was used and detection tests were performed, monitored by PM-RAIRS. The successive functionalization steps were monitored by PM-RAIRS and QCM-D. The binding of the proteins to gold surface, their saturation coverages and association constants, as well as their capture efficiencies were discussed. BaP capture by the antibody layer was evidenced by the appearance of a new (C-H) band at 3039 cm −1 typical of aromatic C-H bonds. The integrated area of this band varied linearly with the BAP concentration within the range of tested concentrations, with a limit of detection close to 3 M. This represents the first example of direct, label-free immunodetection of a low molecular weight molecule by PM-RAIRS transduction. The simplicity and the rapid response of this IR sensor make it already very worthwhile to preliminary on-site measurements.

Surface Science, 2007

Immobilisation of rabbit immunoglobulin G (rIgG) was performed by affinity binding to protein A (... more Immobilisation of rabbit immunoglobulin G (rIgG) was performed by affinity binding to protein A (PrA) covalently bound to three different thiolate self-assembled monolayers (SAMs), (i) a mixed SAM of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) at a molar ratio of 1-3, (ii) a pure SAM of MUA and (iii) a pure SAM of cystamine (CA). A comparative study of anti-rIgG recognition process on these three surfaces was achieved in order to assess the influence of the attachment layer topography and composition upon the sensor quality. Functionalised gold-coated surfaces were characterised by three complementary analytical techniques, namely atomic force microscopy (AFM), polarization modulation-reflection-adsorption infrared spectroscopy (PM-RAIRS) and quartz crystal microbalance (QCM). PM-RAIRS and AFM revealed that the three SAMs were formed on the gold surfaces. AFM observations made it clear that the thiolate and PrA layers were rather homogeneous in the case of pure MUA and CA SAMs, as compared to the MUA/C6OH mixed SAM on which PrA aggregates were observed. Though the highest amount of antibody was bound to the PrA on CA layer, higher anti-rIgG over IgG ratios were measured on the less dense layers of antibody.

Surface and Interface Analysis, 2006

... 11. Pradier CM, Salmain M, Zheng L, Méthivier C. Surf. Interface Anal. 2002; 34: 67. 12. Dunb... more ... 11. Pradier CM, Salmain M, Zheng L, Méthivier C. Surf. Interface Anal. 2002; 34: 67. 12. Dunbar B. J. Agric. ... Chem. 1992; 64: 337. 31. Castner DG, Hinds K, Grainger DW. Langmuir 1996; 12: 5083. 32. Spinke J, Liley M, Guder HJ, Angermaier L, Knoll W. Langmuir 1993; 9: 1821.

Surface and Interface Analysis, 2002

Organometallics, 2013

ABSTRACT Friedel–Crafts acylation of ferrocene with a biotin-derived carboxylic acid having 6-ami... more ABSTRACT Friedel–Crafts acylation of ferrocene with a biotin-derived carboxylic acid having 6-aminohexanoyl linkers attached to the biotin carboxylic group and with desthiobiotin afforded the corresponding ferrocene–biotin bioconjugates. These compounds as well as biotinyl ferrocenyl ketone exhibit high affinity for avidin. Their cytotoxicity against cancer cell lines having various levels of biotin receptors (SMVT) was measured and revealed that lines displaying high levels of SMVT (SW620) were the most susceptible. This suggests that biotin serves as a biological vector delivering cytotoxic ferrocenyl moieties to cancer cells. The crystal structure of the complex of avidin with a conjugate having two linker units between biotin and ferrocene was determined and revealed stabilization of several different conformations of the ligand within the protein binding pocket.

New Journal of Chemistry, 1998

Transition metallo-carbonyl complexes are useful bioprobes to study molecular recognition process... more Transition metallo-carbonyl complexes are useful bioprobes to study molecular recognition processes such as ligand-receptor interactions, owing to their intense absorption bands in the mid-IR spectral range, which enable their detection at the picomole level with FT-IR ...

Langmuir, 2005

A new strategy aiming at the protection of metallic surfaces against the growth of biofilms is pr... more A new strategy aiming at the protection of metallic surfaces against the growth of biofilms is presented here. This work reports the grafting of primary amines by aminosilanization of oxidized stainless steel followed by chemical coupling of the glycosidase lysozyme from hen egg white using glutaraldehyde as homobifunctional cross-linking agent. Controlled characterization of a stainless steel surface by X-ray photoelectron spectroscopy and Fourier transform infrared reflection-absorption spectroscopy at each step enabled the mode of binding, coverage, and orientation of the grafted molecules to be addressed. As a result, the stainless steel samples covered with a covalently immobilized layer of lysozyme showed some lytic activity on a suspension of bacteria Micrococcus lysodeikticus.

Langmuir, 2006

A first example of the solid-phase immunoassay of a high-weight antigen bovine serum albumin (BSA... more A first example of the solid-phase immunoassay of a high-weight antigen bovine serum albumin (BSA) using an (η 5 -cyclopentadienyl)tricarbonylmanganese (cymantrene) redox probe is presented. The electrochemical detection is based on the impedance measurements of a one-electron reversible reduction of the organometallic probe. The microbeadbased immunoassay is discussed for two types of microbeads with different diameters (2.5 and 90 µm) and capabilities to bind the immunoglobulins (2.4 and 10 µg/mg of beads). The use of larger agarose microbeads allows the formation of an antigen-antibody complex at the surface of microbeads directly dispersed in the analyzed solution. No additional separation step is necessary for the electrochemical competitive immunoassay analysis of BSA. The presence of agarose beads in the analyzed solution has no effect on the electrochemical signal from labeled BSA released from the antigen-antibody complex.

Journal of Organometallic Chemistry, 2005

Syntheses of Fischer carbene complexes with various hydrophilic tethers (4, 6a, 6b, 6c, 8a, 8b, 1... more Syntheses of Fischer carbene complexes with various hydrophilic tethers (4, 6a, 6b, 6c, 8a, 8b, 14a, 14b, 15 and 19) are described. The water-soluble carbene complex, 14b, was used to label and pegylate bovine serum albumin (BSA) without affecting its conformation. The red complex, 19, underwent a sharp color change to yellow on reaction with BSA, a feature that is potentially useful for developing assay methods.

Journal of Organometallic Chemistry, 2009

Dialkyl- and diphenyl phosphites react with the (η5-C5H5)M(CO)x(η1-N-maleimidato) (M=Fe, Mo; x=2 ... more Dialkyl- and diphenyl phosphites react with the (η5-C5H5)M(CO)x(η1-N-maleimidato) (M=Fe, Mo; x=2 or 3) complexes giving products of the phospha-Michael addition to the η1-N-maleimidato ligand. One of these complexes (M=Fe, x=2) was characterized by X-ray diffraction. The synthesized metallocarbonyl azaphosphonates and the corresponding iron phosphonic acid act as inhibitors of certain serine hydrolases (AChE and BChE). The kinetic assays were performed

Journal of Molecular Catalysis A: Chemical, 2003

ABSTRACT The synthesis, through a cross-metathesis reaction, of the first chiral peptide nucleic ... more ABSTRACT The synthesis, through a cross-metathesis reaction, of the first chiral peptide nucleic acid (PNA) monomer labelled with a Fischer-type carbene complex of chromium is reported. IR analysis of the new bioconjugate shows that the Cr(CO)4 moiety represents a suitable spectroscopic probe for diagnostic purposes.

Journal of Inorganic Biochemistry, 2009

Recently we have found that the metallocarbonyl complexes (g 5 -C 5 H 5 )M(CO) x (g 1 -N-maleimid... more Recently we have found that the metallocarbonyl complexes (g 5 -C 5 H 5 )M(CO) x (g 1 -N-maleimidato) (M = Fe, Mo, W; x = 2 or 3) bearing a maleimide function were irreversible inhibitors of the enzyme papain. To get further insight into the binding mechanism of these compounds we synthesized the related complexes (g 5 -C 5 H 5 )M(CO) x (g 1 -N-succinimidato) (M = Fe, Mo, W; x = 2 or 3) that lacked the ethylenic bond responsible for alkylation of the cysteine 25 thiol group in the papain's catalytic pocket. We performed kinetic studies of the interaction of the synthesized complexes towards papain. We found that they act as reversible inhibitors of the enzyme with IC 50 values in the range 480-1700 lM. Docking experiments confirmed binding of these complexes to the enzyme's catalytic pocket.

Journal of Colloid and Interface Science, 2010

Two hydrolytic enzymes, namely lysozyme and trypsin, were covalently immobilized onto stainless s... more Two hydrolytic enzymes, namely lysozyme and trypsin, were covalently immobilized onto stainless steel surfaces using wet chemistry processes. The immobilization strategy took advantage of the spontaneous physisorption of the polymer poly(ethylene imine) (PEI) onto stainless steel to yield a firmly attached, thin organic layer containing a high density of primary amine functions. Both enzymes were then covalently grafted to the surface via a glutaraldehyde cross-linker. Alternatively, a thicker underlayer of PEI was chemisorbed by cross-linking two PEI layers by glutaraldehyde. The effective presence of both enzymes on the stainless steel surfaces and their relative amount were assessed by immunochemical assays employing specific anti-enzyme antibodies. Eventually, the hydrolytic activity of the immobilized enzymes was evaluated by local enzymatic tests with suitable substrates. This work demonstrates that, although the amount of enzymes did not vary significantly with the underlayer thickness, their hydrolytic activity could be much improved by increasing the distance from the oxide surface and, likely, by favoring their accessibility. Our data suggest that the immobilization of enzymes on solid oxide surfaces is feasible and efficient, and that the enzymes retain catalytic activity. It may thus provide a promising route towards biofilm-resistant materials.

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Journal of Chemical Crystallography, 1996

... To complete the studies on this family of complexes, we describe herein the X-o) Institut de ... more ... To complete the studies on this family of complexes, we describe herein the X-o) Institut de Chimie, Universit6 de Constantine, Route d'Ain El Bey, 25000 Constantine, Algeria. ... Radiation, graphite monochromator MoK~ (k = 0.71073) MoK, (k = 0.71073) ...

Dalton Transactions, 2010

Covalent embedding of a (eta(6)-arene) ruthenium(II) complex into the protein papain gives rise t... more Covalent embedding of a (eta(6)-arene) ruthenium(II) complex into the protein papain gives rise to a metalloenzyme displaying a catalytic efficiency for a Lewis acid-mediated catalysed Diels-Alder reaction enhanced by two orders of magnitude in water.

Comptes Rendus Chimie, 2005

Tricarbonyl cyclohexa-1,3-diene iron(0) derivatives of two biologically active molecules, N-acety... more Tricarbonyl cyclohexa-1,3-diene iron(0) derivatives of two biologically active molecules, N-acetyl cysteine and N-acetyl histamine and of a primary amine, n-butylamine, have been prepared by reaction of [tricarbonyl (1-4-g-5-N-pyridiniocyclohexa-1,3-diene) iron] tetrafluoroborate , a precursor of the highly reactive cation [Fe(CO) 3 (1-5-g-C 6 H 7 )](+), and characterized spectroscopically. Kinetic studies revealed that the reaction of N-acetyl histamine and n-butylamine was much faster than the reaction of N-acetyl cysteine. Acid/base titration of metal-carbonyl labelled histamine was achieved by IR spectroscopy and revealed that the imidazole ring substituted by the metal-carbonyl unit was slightly more basic than the parent compound. To cite this article: M. Mokhtari et al., C. R. Chimie 8 (2005).

Collection of Czechoslovak Chemical Communications, 2001

ABSTRACT The mechanisms of electrochemical reduction of cymantrene, [Mn(CO)3(η5-Cp)], and its rin... more ABSTRACT The mechanisms of electrochemical reduction of cymantrene, [Mn(CO)3(η5-Cp)], and its ring-substituted methyl carboximidate derivative, [Mn(CO)3(η5-C5H4C(NH)OMe)], were studied by voltammetry, in situ IR spectroelectrochemistry and preparative electrolysis. The product of one-electron reduction undergoes further chemical reactions. Comparison of the data obtained under atmosphere of argon and that of carbon monoxide leads to the conclusion that a ligand substitution reaction and dimerization participate in the overall reaction sequence. FTIR spectra recorded in situ suggest product dimerization, the formation of [Mn(CO)5]- and, to a lesser extent, other unstable species. The dimer formation was not observed in the course of the reduction of the carboximidate.