Michał Marszałł - Academia.edu (original) (raw)

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Papers by Michał Marszałł

Medical Research Journal, 2016

Medical Research Journal, 2015

Thrombosis and haemostasis, Jan 15, 2016

Aim of this study was assessment of the relationship between concentrations of ticagrelor and its... more Aim of this study was assessment of the relationship between concentrations of ticagrelor and its active metabolite (AR-C124910XX) and results of selected platelet function tests. In a single-centre, cohort study, patients with myocardial infarction underwent blood sampling following a 180 mg ticagrelor loading dose intake (predose, 1, 2, 3, 4, 6, 12, 24 hours postdose) to perform pharmacokinetic and pharmacodynamic assessments. Platelet reactivity was evaluated using the VASP-assay, the VerifyNow device and the Multiplate analyzer. Analysis of 36 patients revealed high negative correlations between ticagrelor concentrations and platelet reactivity evaluated with all three platelet function tests (the VASP-assay: RS=-0.722; p<0.0001; the VerifyNow device: RS=-0.715; p<0.0001; the Multiplate analyzer: RS=-0.722; p<0.0001), with no significant differences between correlation coefficients. Similar results were found for AR-C124910XX. Platelet reactivity values assessed with al...

Journal of Molecular Catalysis B: Enzymatic, 2016

European Journal of Cancer Prevention, 2016

Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen l... more Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.

Journal of Pharmaceutical and Biomedical Analysis, 2016

Superparamagnetic nanoparticles with chemically modified chitosan has been proposed as a potentia... more Superparamagnetic nanoparticles with chemically modified chitosan has been proposed as a potential support for the immobilization of the androgen receptor (AR). The study involved comparison of different AR carriers like commercially available magnetic beads coated with silica (BcMag) and chitosan coated nanoparticles with different amount of amino groups. The immobilization was carried out through covalent immobilization of the AR through the terminal amino group or through available carboxylic acids. The initial characterization of the AR coated magnetic beads was carried out with dihydrotestosterone, a known AR ligand. Subsequently, chitosan modified nanporticles with long-distanced primary amino groups (Fe3O4CS-(NH2)3) (upto 8.34mM/g) were used for further study to isolate known AR ligands (bicalutamide, flutamide, hydroxyflutamide and levonogestrel) from a mixture of tested compounds in ammonium acetate buffer [10mM, pH 7.4]. The results showed that the selected nanoparticles are a promising semi-quantitative tool for the identification of high affinity compounds to AR and might be of special importance in the identification of novel agonists or antiandrogens.

Journal of Pharmaceutical and Biomedical Analysis, Jul 8, 2010

A simple and fast method for the determination of the association constant (K(a)) of ligand to hu... more A simple and fast method for the determination of the association constant (K(a)) of ligand to human serum albumin (HSA) has been developed by using human serum albumin-coated magnetic beads (HSA-MB). To date, magnetic beads (MB) have been increasingly used as a bioseparation tool, especially for DNA, RNA, protein, enzyme and cell isolation or purification. In this study, HSA-MB were used as a new tool to determine the affinity of known ligands to HSA. The K(a) for l-tryptofan, fenoprofen, ketoprofen, tolbutamide and warfarin obtained from Schathard analysis are consistent with previously reported values. The different K(a) values for ketoprofen after the acetylation of HSA-MB by preincubation with acetylosalicylic acid indicate that these beads can be successfully adapted in combined experiment. In addition, the HSA-MB experiment with phenytoin and valproic acid proved to be a simple method to examine drug displacement effect.

Analytical Biochemistry, Feb 15, 2008

J Liq Chromatogr Relat Techno, 2012

The determination of lamotrigine in tablets using high-performance thin-layer chromatography (HPT... more The determination of lamotrigine in tablets using high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC) as well as direct, first, second and third derivative ultraviolet spectrophotometry methods are described. HPTLC method was performed using the silica plates, mobile phase composed of toluene:acetone:ammonia (3:7:0.5, v/v/v) and densitometric detection at 312 nm. Moreover, quantification was achieved in the concentration range of 0.25-10.0 µg/spot and with good precision (RSD = 2.19%) and recovery (97.92%) using non-linear calibration curve by fitting to y = a+blnx. HPLC method was performed using Luna C18 column and isocratic elution mode with a mobile phase composed of methanol:phosphate buffer (25:75, v/v) and delivered at a flow rate 1.2 ml/min. Proposed HPLC method provided good results of precision (RSD = 1.86%) and recovery (101.51%) in the concentration range of 0.25-7.5 µg/ml using linear y = a+bx regression analysis. For UV-derivative spectrophotometry method all derivatives and wavelengths studied proved the accurate linearity, precision (RSD = 0.96-1.87%) and recovery (96.74-101.90%). Moreover, no interference was found from tablet excipients at the selected wavelength and assay procedures. The developed methods were found to be validated and showed good precision and reproducibility.

Http Dx Doi Org 10 1080 10408340601107847, Apr 19, 2007

Journal of Liquid Chromatography Amp Related Technologies, Mar 13, 2014

Jpc J Planar Chromat Mod Tlc, 2003

European Heart Journal, 2015

The currently available data indicate a drug-drug interaction between morphine and oral P2Y12 rec... more The currently available data indicate a drug-drug interaction between morphine and oral P2Y12 receptor inhibitors, when administered together. The aim of this trial was to assess the influence of infused morphine on pharmacokinetics and pharmacodynamics of ticagrelor and its active metabolite (AR-C124910XX) in patients with acute myocardial infarction. In a single-centre, randomized, double-blind trial, patients were assigned in a 1:1 ratio to receive intravenously either morphine (5 mg) or placebo, followed by a 180 mg loading dose of ticagrelor. Pharmacokinetics was determined with liquid chromatography tandem mass spectrometry and ticagrelor antiplatelet effects were measured with up to three different platelet function tests: vasodilator-stimulated phosphoprotein phosphorylation assay, multiple electrode aggregometry and VerifyNow. The pharmacokinetic and pharmacodynamic assessment was performed in 70 patients (35 in each study group). Morphine lowered the total exposure to ticagrelor and its active metabolite by 36% (AUC(0-12): 6307 vs. 9791 ng h/mL; P = 0.003), and 37% (AUC(0-12): 1503 vs. 2388 ng h/mL; P = 0.008), respectively, with a concomitant delay in maximal plasma concentration of ticagrelor (4 vs. 2 h; P = 0.004). Multiple regression analysis showed that lower AUC(0-12) values for ticagrelor were independently associated with the administration of morphine (P = 0.004) and the presence of ST-segment elevation myocardial infarction (P = 0.014). All three methods of platelet reactivity assessment showed a stronger antiplatelet effect in the placebo group and a greater prevalence of high platelet reactivity in patients receiving morphine. Morphine delays and attenuates ticagrelor exposure and action in patients with myocardial infarction. ClinicalTrials.gov Identifier: NCT02217878.

The Delivery of Nanoparticles, 2012

The FASEB Journal

ABSTRACT Meeting Abstract Supplement 756.1

The International Journal of Biological Markers, 2015

Because of the numerous limitations of prostate-specific antigen (PSA), α-methylacyl-CoA racemase... more Because of the numerous limitations of prostate-specific antigen (PSA), α-methylacyl-CoA racemase (AMACR) and hepsin have recently been suggested as potential biomarkers in prostate cancer (PC). This report presents a comparison study of the presence of AMACR and hepsin in urine collected before and after digital rectal examination (DRE) as a previously suggested diagnostic marker for PC. Seventy-six urine samples (38 before and 38 after prostate massage) from patients with benign prostate hyperplasia (BPH) and 66 urine samples (33 before and 33 after prostate massage) from patients with PC were analyzed. PC was confirmed by prostate biopsy. Urinary levels of AMACR and hepsin were determined by ELISA and related to the tumor stage, Gleason score and PSA level. AMACR and hepsin levels in urine collected after prostate massage were higher only in the PC group. There were no correlations between AMACR levels, hepsin levels, tumor stage and Gleason score. AMACR and hepsin did not differentiate between BPH and PC with better true positive and false negative rates than serum PSA. AMACR and hepsin were unable to diagnose PC with better true positive and false negative rates than PSA. An additional procedure combined with other markers should be applied for the reliable diagnosis of PC.

Central European Journal of Medicine, 2012